Abstract:
:Single-cell technologies are powerful tools to evaluate cell characteristics. In particular, Atomic Force Microscopy (AFM) nanoindentation experiments have been widely used to study single cell mechanical properties. One important aspect related to single cell techniques is the need for sufficient statistical power to obtain reliable results. This aspect is often overlooked in AFM experiments were sample sizes are arbitrarily set. The aim of the present work was to propose a tool for sample size estimation in the context of AFM nanoindentation experiments of single cell. To this aim, a retrospective approach was used by acquiring a large dataset of experimental measurements on four bone cell types and by building saturation curves for increasing sample sizes with a bootstrap resampling method. It was observed that the coefficient of variation (CV%) decayed with a function of the form y = axb with similar parameters for all samples tested and that sample sizes of 21 and 83 cells were needed for the specific cells and protocol employed if setting a maximum threshold on CV% of 10% or 5%, respectively. The developed tool is made available as an open-source repository and guidelines are provided for its use for AFM nanoindentation experimental design.
journal_name
J Mech Behav Biomed Materauthors
Marcotti S,Reilly GC,Lacroix Ddoi
10.1016/j.jmbbm.2019.03.018subject
Has Abstractpub_date
2019-06-01 00:00:00pages
259-266eissn
1751-6161issn
1878-0180pii
S1751-6161(18)30838-5journal_volume
94pub_type
杂志文章abstract::New processing methods for shape-memory polymers allow for tailoring material properties for numerous applications. Shape-memory nonwovens have been previously electrospun, but melt blow processing has yet to be evaluated. In order to determine the process parameters affecting shape-memory behavior, this study examine...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.05.038
更新日期:2016-09-01 00:00:00
abstract::In the present work, mechano-geometrical characterisations of skeletal muscle fibres in two different deformation states, namely, axial tension and axial compression, were realised. In both cases, cyclic and relaxation tests were performed. Additionally, the changes in the volume of the fibres during deformation were ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.104001
更新日期:2020-10-01 00:00:00
abstract::Main objective of this study is to investigate the thermal behavior of ceramic laminate veneer restorations of the maxillary central incisor with different incisal preparations such as butt joint and palatinal chamfer using finite element method. In addition, it is also aimed to understand the effect of different ther...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.07.039
更新日期:2017-11-01 00:00:00
abstract::In the present study, inflation tests with free axial extension of 15 human vena saphena magna were conducted ex vivo to obtain data suitable for multi-axial constitutive modeling at overloading conditions (pressures up to approximately 15kPa). Subsequently the data were fitted with a hyperelastic, nonlinear and aniso...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.01.023
更新日期:2015-05-01 00:00:00
abstract::The AFM combined nanoindentation was performed to observe the ultrastructure of enamel rod from various section plans and positions while probing their mechanical and tribological properties of the area. The nanohardness and the elastic modulus of the head region of the enamel rods are significantly higher than that o...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2010.12.002
更新日期:2011-05-01 00:00:00
abstract::The scaled skin of fish is an intricate system that provides mechanical protection against hard and sharp puncture, while maintaining the high flexural compliance required for unhindered locomotion. This unusual combination of local hardness and global compliance makes fish skin an interesting model for bioinspired pr...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.06.014
更新日期:2017-12-01 00:00:00
abstract::The aim of the presented study was to estimate the material properties of human patellar ligaments from the elderly population by means of tensile tests. The experimental part was conducted on a custom tensile-testing device, with a built-in enclosure to simulate in-vivo conditions, using 25 (15 female, 10 male) bone-...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.103994
更新日期:2020-10-01 00:00:00
abstract::The wear properties of oxidized and non-oxidized gamma-TiAl (a potential biomaterial) as well as Ti-6Al-4V and CP-Ti disks were studied and characterized by means of standard wear tests using a custom made bone pin arrangement. The Ti-based disks were oxidized in air at 500 and 800 degrees C for one hour. The tribolog...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2010.06.006
更新日期:2010-11-01 00:00:00
abstract::A facile procedure has been devised to develop a novel dentin bonding system containing poly (acrylic acid)-grafted-silanized fumed silica particles as reinforcing filler, with high stability of nanoparticle dispersion and enhanced bond strength and mechanical properties. In the first step, the silanization of fumed s...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.103904
更新日期:2020-10-01 00:00:00
abstract::Capsules consist of droplets enclosed by a membrane with shear resistant properties especially when fabricated by interfacial cross-linking. In many applications, the protection and release of the internal medium need to be strictly controlled. It is possible to tune the membrane mechanical properties by changing the ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.09.008
更新日期:2016-05-01 00:00:00
abstract::During an underbody blast (UBB) event, mounted occupants are exposed to high rate loading of the spine via the pelvis. The objective of this study was to simulate UBB loading conditions and examine mechanisms of injury in the thoracic, lumbar and sacral spine. Fourteen instrumented, whole-body, postmortem human subjec...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.104271
更新日期:2020-12-24 00:00:00
abstract::We have an interest in the cellular response to mechanical stimuli, and here describe an in-vitro method to examine the response of cells cultured in a three-dimensional matrix to mechanical compressive and tensile stress. Synthetic aliphatic polyester scaffolds coated with 45S5 bioactive glass were seeded with human ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2018.08.006
更新日期:2018-12-01 00:00:00
abstract::For three decades, low level laser therapy (LLLT) has been used for treatment of tendinitis as well as other musculoskeletal diseases. Nevertheless, the biological mechanisms involved remain not completely understood. In this work, the effects of LLLT and of the widely used nonsteroidal anti-inflammatory drug, diclofe...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2013.08.028
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:To compare osseointegration and implant stability of two types of laser-etched (LE) Ti implants with a chemically-modified, sandblasted, large-grit and acid-etched (SLA) Ti implant (SLActive(®), Straumann, Basel, Switzerland), by evaluating removal torque and resonance frequency between the implant surface and ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.03.034
更新日期:2016-08-01 00:00:00
abstract::The current study deals with the preparation and the characterization of the PVA-CMT-GO films. The PVA-CMT film was translucent in nature and smooth to touch. The addition of GO resulted in the formation of agglomerated structures. XRD studies suggested that the incorporation of GO increased the average crystallite si...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2018.02.021
更新日期:2018-05-01 00:00:00
abstract::Mechanical assessment of biological materials and tissue-engineered scaffolds is increasingly focusing at lower length scale levels. Amongst other techniques, atomic force microscopy (AFM) has gained popularity as an instrument to interrogate material properties, such as the indentation modulus, at the microscale via ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2018.05.026
更新日期:2018-09-01 00:00:00
abstract::Many treatments for cardiovascular diseases include an endovascular insertion of stents or stent grafts into arteries, a procedure which may cause high tissue stresses and even damage in the arterial wall. In order to study such problems by using finite element methods, both appropriate constitutive models and experim...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2012.03.012
更新日期:2012-08-01 00:00:00
abstract::Bone derives its mechanical strength from the complex arrangement of collagen fibrils (type-I primarily) reinforced with hydroxy-apatite (HAp) mineral crystals in extra- and intra-fibrillar compartments. This study demonstrates a novel approach to obtain organic matrix of bone through its demineralization as well as m...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2018.12.031
更新日期:2019-03-01 00:00:00
abstract::High brittleness and lack osteogenesis are two major limitations of calcium phosphate cement (CPC) in application in bone defect reconstruction. Here we prepared a composite calcium phosphate cement by mixing N-acetyl cysteine loaded silk fibroin solution with α-tricalcium phosphate. In vitro cytology experiment revea...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2019.103451
更新日期:2020-01-01 00:00:00
abstract::Considering the interest of the research community in the fatigue behavior of all-ceramic restorations and the time consumed in low-frequency cyclic fatigue tests, this study aimed to investigate the influence of the loading frequency on the zirconia fatigue strength. The biaxial flexural fatigue strength of Y-TZP dis...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.01.008
更新日期:2016-08-01 00:00:00
abstract::The shape memory effect (SME) has long been the focus of interest of many research groups that have studied many facets of it, yet to the authors' knowledge some molecular parameters, such as the molecular weight, have been skipped. Thus, the aim of this work is to offer further insight into the shape memory effect, b...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2016.04.027
更新日期:2016-08-01 00:00:00
abstract::Orthopedic interfaces such as the tendon-bone junction (TBJ) present unique challenges for biomaterials development. Here we describe a multi-compartment collagen-GAG scaffold fabricated via lyophilization that contains discrete mineralized (CGCaP) and non-mineralized (CG) regions joined by a continuous interface. Mod...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2013.07.016
更新日期:2013-12-01 00:00:00
abstract::Osteoporosis can lead to bone compressive fractures in the lower lumbar vertebrae. In order to assess the recovery of vertebral strength during drug treatment for osteoporosis, it is necessary not only to measure the bone mass but also to perform patient-specific mechanical analyses, since the strength of osteoporotic...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2009.03.001
更新日期:2010-01-01 00:00:00
abstract::Proper statistical analysis is essential in the research studies. In particular, as regards the in vitro testing of the lumbar spine, the criteria for the standardization have been extensively discussed but the use of statistics has not been reviewed. Unfortunately, cadaveric testing is a very difficult and complex ex...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2020.103862
更新日期:2020-10-01 00:00:00
abstract::One of the most important alterations that occur in man and experimental animals during spaceflight concerns the skeletal system, and entails important bone loss and degradation of mechanical properties. In the present work we investigate ex vivo the long-term effects of weightlessness (simulated microgravity) on bone...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2015.01.002
更新日期:2015-04-01 00:00:00
abstract::Dentin restoration produces weak interfaces because of the effects of bacterial microflora, biofilms, and mechanical, thermal, and shrinkage stresses. This results in secondary caries. Therefore, hydrophobic elastic polyurethane (PU) containing different concentrations of triclosan derivatives was synthesized and appl...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2019.103471
更新日期:2020-02-01 00:00:00
abstract::Hydrogels are more and more attractive in biomedical fields, since they can be used as injectable scaffolds, drugs and gene carriers and smart sensors. The highly hydrated hydrogels, however, generally have low mechanical strength. In this work, a composite chitosan hydrogel was prepared by blending water soluble and ...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2008.02.001
更新日期:2008-10-01 00:00:00
abstract::The present study has two major purposes: firstly, to investigate whether the analytical model proposed by Jedwab and Clerc for assessing the mechanical behaviour of an open ends metallic braided stent is applicable to the looped ends stent design and secondly, to compare the response of the two stent designs subjecte...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2017.08.025
更新日期:2017-11-01 00:00:00
abstract::A series of computational studies were performed to investigate the response of the lower extremities of mounted soldiers under landmine detonation. A numerical human body model newly developed at Wayne State University was used to simulate two types of experimental studies and the model predictions were validated aga...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2013.07.010
更新日期:2013-12-01 00:00:00
abstract::Skeletal abnormalities reported in humans and laboratory animals after spaceflight, include cancellous osteopenia, decreased cortical and cancellous bone formation, aberrant matrix ultrastructure, decreased mineralization and reduced bone strength. Although considerable effort has been made up to now to understand the...
journal_title:Journal of the mechanical behavior of biomedical materials
pub_type: 杂志文章
doi:10.1016/j.jmbbm.2008.06.004
更新日期:2009-04-01 00:00:00