Abstract：
:The transient silencing effects currently demonstrated by nonviral siRNA delivery systems limit the therapeutic utility of RNAi, but it remains a technical challenge to prolong duration of gene silencing. We have developed a T7 autogene-based hybrid mRNA/DNA system to enable long-term expression of shRNA in cytoplasm in vitro and in vivo. This hybrid mRNA/DNA system consists of T7 polymerase (T7pol) mRNA, pT7/shRNA-encoding DNA fragment and T7 autogene plasmid, and it can generate higher levels of T7pol proteins, compared to pCMV-triggering T7 autogene system, especially without the need of nuclear entry of any gene. A large amount of T7pol proteins produced are used to induce pT7-driven expression of shRNA in cytoplasm, and through cellular processing of RNA hairpins, mature siRNAs are generated for more than 13 days. We here demonstrate that a single liposomal delivery of this hybrid system leads to the long-term silencing effects in vitro and in vivo, in contrast to the conventional siRNA methods relying on the repeated administrations every 2 or 3 days. These sustainable shRNA expression properties in cytoplasm can provide an efficient strategy to address the limitations caused by shRNA-encoding plasmid DNA systems such as low nuclear entry efficiency and short-term silencing effect. The development of long-term shRNA expression system in vivo could scale down administration frequency of RNAi therapeutics in the treatment of chronic diseases, thereby increasing its clinical utility.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kwak SY,Han HD,Ahn HJdoi
10.1038/s41598-019-39407-8subject
Has Abstractpub_date
2019-02-28 00:00:00pages
2993issue
1issn
2045-2322pii
10.1038/s41598-019-39407-8journal_volume
9pub_type
杂志文章abstract：:Systemic lipopolysaccharide (LPS) is implicated in increasing mortality in patients with alcoholic hepatitis but the underlying mechanisms are not well characterised. The objective of this study was to characterise neutrophil function, LPS and cytokine concentrations within the splanchnic circulation of alcoholic cirr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40157
更新日期：2017-01-04 00:00:00
abstract：:The charge transfer from the main catalyst to the cocatalyst is a key factor to enhance catalytic activity for photocatalytic nanocomposite materials. In order to enhance the charge transfer between Bi2WO6 and graphene, we inlet MoS2 as a "stepping-stone" into Bi2WO6 and graphene. Here, we report an effective strategy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03911-6
更新日期：2017-06-16 00:00:00
abstract：:Because of the lack of cell wall, Micoplasma species require a fine control of membrane fluidity and integrity. mg517 is an essential gene of Mycoplasma genitalium responsible for the biosynthesis of membrane glycoglycerolipids. It encodes for a unique glycosyltransferase (MG517) with processive activity, transferring...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42970-9
更新日期：2019-05-08 00:00:00
abstract：:Adult neurogenesis occurs in the dentate gyrus of the hippocampus during adulthood and contributes to sustaining the hippocampal formation. To investigate whether neurogenesis-related pathways are associated with hippocampal volume, we performed gene-set enrichment analysis using summary statistics from a large-scale ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50507-3
更新日期：2019-10-10 00:00:00
abstract：:This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80764-6
更新日期：2021-01-14 00:00:00
abstract：:Neonatal mice exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). Cardiac biomechanics intricately affect long-term heart function, but whether regenerated cardiac muscle is biomechanically similar to native myocardium remains u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63324-w
更新日期：2020-04-30 00:00:00
abstract：:Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing (+B) individuals of F. pratensis. 61% of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56383-1
更新日期：2019-12-27 00:00:00
abstract：:Silver nanobelts (AgNBs) have attracted a great interest due to their excellent electrical conductivity and mechanical strength, leading a facile synthesis of these AgNBs in great demand. In here, we are reporting a simple, aqueous phase, size tunable synthesis of smooth surfaced 1D-silver nanobelts using a Polyanilin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44796
更新日期：2017-03-20 00:00:00
abstract：:The triethylamine promoted three-component reaction of N-(4-nitrobenzyl), N-ethoxycarbonylmethylisoquinolinium bromide, isatins and malononitrile in ethanol afforded spiro[indoline-3,2'-pyrrolo[2,1-a]isoquinolines] in good yields and with high diastereoselectivity. The similar reaction of N-cyanomethylisoquinolinium c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41024
更新日期：2017-01-20 00:00:00
abstract：:In the present study, we obtained population genetic data and forensic parameters of 30 InDel loci in Chinese Xibe ethnic group from northwestern China and studied the genetic relationships between the studied Xibe group and other reference groups. The observed heterozygosities ranged from 0.1704 at HLD118 locus to 0....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08260
更新日期：2015-02-05 00:00:00
abstract：:Gradient Mn-La-Pt catalysts with three-layered structure of manganese dioxide (MnO2), lanthanum oxide (La2O3), and Platinum (Pt) for Li-O2 battery are prepared in this study. The mass ratio of the catalysts is respectively 5:2:3, 4:2:4, and 3:2:5 (MnO2: La2O3: Pt) which is start from the side of the electrolyte. The r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34950
更新日期：2016-10-12 00:00:00
abstract：:Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen colonization using traditiona...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25434-4
更新日期：2018-05-09 00:00:00
abstract：:Duchenne muscular dystrophy, one of the most common lethal genetic disorders, is caused by mutations in the DMD gene and a lack of dystrophin protein. In most DMD patients and animal models, sporadic dystrophin-positive muscle fibres, called revertant fibres (RFs), are observed in otherwise dystrophin-negative backgro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38371
更新日期：2016-12-07 00:00:00
abstract：:Detailed measurements of physical, chemical and biological dynamics in groundwater are key to understanding the important processes in place and their influence on water quality - particularly when used for drinking water. Measuring temporal bacterial dynamics at high frequency is challenging due to the limitations in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38462
更新日期：2016-12-07 00:00:00
abstract：:A major translational challenge in the fields of therapeutic angiogenesis and regenerative medicine is the need to create functional microvasculature. The purpose of this study was to assess whether a potentially autologous endothelial cell (EC) source derived from human induced pluripotent stem cells (iPSC-ECs) can f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20966-1
更新日期：2018-02-08 00:00:00
abstract：:Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as biofilms, are associated with several infectious diseases. One of the main components of these biofilms is alginate, a homo- and hetero-polysaccharide that consists of β-D-mannuronate (M) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66293-2
更新日期：2020-06-10 00:00:00
abstract：:Pro-inflammatory and amyloidogenic S100A9 protein is an important contributor to Alzheimer's disease (AD) pathology. Traumatic brain injury (TBI) is viewed as a precursor state for AD. Here we have shown that S100A9-driven amyloid-neuroinflammatory cascade was initiated in TBI and may serve as a mechanistic link betwe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31141-x
更新日期：2018-08-27 00:00:00
abstract：:The study was aimed to investigate the pathogens distribution and risk factors for PICC-related bloodstream infection in intensive care unit (ICU) patients. 402 patients placed with PICC in ICU were recruited in the study. The microbiological characteristics of PICC-related infection were investigated by Vitek 2 Compa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10037-2
更新日期：2017-11-08 00:00:00
abstract：:The volcanic eruption of Santorini in the Bronze Age left detectable debris across the Mediterranean, serving as an anchor in time for the region, synchronizing chronologies of different sites. However, dating the eruption has been elusive for decades, as radiocarbon indicates a date about a century earlier than archa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79024-4
更新日期：2021-01-12 00:00:00
abstract：:We evaluated regression of iris neovascularization (INV) using en-face anterior-segment optical coherence tomography angiography (AS-OCTA) after anti-vascular endothelial growth factor (VEGF) therapy. Seven consecutive eyes with INV were examined before and after anti-VEGF therapy, and all AS-OCTA scans were obtained ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46514-z
更新日期：2019-07-16 00:00:00
abstract：:Interference of two combined white light beams produces Newton colors if one of the beams is retarded relative to the other by from 400 nm to 2000 nm. In this case the corresponding interfering spectral components are added as two scalars at the beam combination. If the retardance is below 400 nm the two-beam interfer...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17340
更新日期：2015-11-27 00:00:00
abstract：:Precise mitotic spindle orientation is essential for both cell fate and tissue organization while defects in this process are associated with tumorigenesis and other diseases. In most animal cell types, the dynein motor complex is anchored at the cell cortex and exerts pulling forces on astral microtubules to position...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24682-8
更新日期：2018-04-20 00:00:00
abstract：:Despite the efficacy of synthetic fungicides in controlling postharvest diseases, public concerns regarding chemical residues in food and an increase in drug-resistant strains of pathogens have led to a need for new agents to control postharvest diseases. The current study was performed to find control agents of micro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49779-6
更新日期：2019-09-19 00:00:00
abstract：:Constrained by the diffraction limit, a lens can only resolve features larger than half of the incident wavelength owing to the decaying nature of evanescent waves. Several novel devices have been proposed, for example, superlenses and hyperlenses to break this limit. In this work, we present a flat hyperlens composed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62243-0
更新日期：2020-03-24 00:00:00
abstract：:We describe a new species of an ornithischian dinosaur, Isaberrysaura mollensis gen. et sp. nov. The specimen, consisting in an almost complete skull and incomplete postcranium was collected from the marine-deltaic deposits of the Los Molles Formation (Toarcian-Bajocian), being the first reported dinosaur for this uni...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42778
更新日期：2017-02-16 00:00:00
abstract：:RT-qPCR offers high sensitivity, for accurate interpretations of qPCR results however, normalisation using suitable reference genes is fundamental. Androgens can regulate transcriptional expression including reference gene expression in prostate cancer. In this study, we evaluated ten mRNA and six non-protein coding R...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19458-z
更新日期：2018-01-31 00:00:00
abstract：:P-type doping in high Al-content AlGaN alloys is a main challenge for realizing AlGaN-based deep ultraviolet optoelectronics devices. According to the first-principles calculations, Mg activation energy may be reduced so that a high hole concentration can be obtained by introducing nanoscale (AlN)5/(GaN)1 superlattice...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44223
更新日期：2017-03-14 00:00:00
abstract：:Blueberry juice is a healthy and nutritious food that has become increasingly popular worldwide. However, little is known about the microbial groups of this juice that can cause its spoilage. This study aimed to identify the main spoilage microorganisms in blueberry juice and explore whether a microchip pulsed electri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26513-2
更新日期：2018-05-25 00:00:00
abstract：:Plant defensins (PDFs) are cysteine-rich peptides that have a range of biological functions, including defence against fungal pathogens. However, little is known about their role in defence against bacteria. In this study, we showed that the protein encoded by ARABIDOPSIS THALIANA PLANT DEFENSIN TYPE 1.1 (AtPDF1.1) is...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08497-7
更新日期：2017-08-23 00:00:00
abstract：:Although an increasing interest in vision training for sport performance, whether it may have a transfer to sport-specific skills and whether such transfer could be mediated by cognition remain open issues. To enlighten this point, we tested the effect of 6-weeks sport vision training programmes (requiring generic or ...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-55252-1
更新日期：2019-12-10 00:00:00
