Abstract：
:PAP80-2a, purified from Pholiota adiposa mycelia, is a polysaccharide exhibiting prominent antitumor effects. However, the yield of PAP80-2a was low and its structure has not been characterized, impeding the exploration of its structure-function relationship, thus influencing the development of oral drugs for antitumor therapy and immunomodulation. In order to improve the yield of PAP80-2a, response surface methodology along with Box-Behnken design was applied to optimize the ultrasonic-assisted extraction conditions for polysaccharides. Then, the structure of PAP80-2a exhibiting antitumor activity was determined from different angles. The results showed that the extraction yield of P. adiposa polysaccharides increased by 11.5% under optimized ultrasonic extraction conditions. Structural analysis showed that PAP80-2a was mainly composed of glucose, rhamnose, xylose, and galactose in a ratio of 10.00: 2.09: 4.09: 1.13. The total amino acid content in the sugar chain was 69.92 μg/mL. The sugar chain structure was [α-Rha (1 → 3)-]n, and rhamnose was located at the non-reducing end of the sugar chain, while glucose was located at the non-reducing end or in the sugar chain in 1,2,6- and 1,3,6-linked forms. Our study clearly illuminates the primary structure of PAP80-2a, but 3D structure of PAP80-2a and its structure-function relationship is a future challenge.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zou Y,Du F,Hu Q,Wang Hdoi
10.1038/s41598-018-38251-6subject
Has Abstractpub_date
2019-02-11 00:00:00pages
1724issue
1issn
2045-2322pii
10.1038/s41598-018-38251-6journal_volume
9pub_type
杂志文章abstract：:Distillers grains are co-products of the corn ethanol industry widely used in animal feed. We examined the effects of erythromycin, penicillin, and virginiamycin at low concentrations reflective of those detected in distillers grains on bacterial resistance selection. At 0.1 µg/ml erythromycin, macrolide-resistant mut...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09593-4
更新日期：2017-09-08 00:00:00
abstract：:Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluid...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36440
更新日期：2016-11-07 00:00:00
abstract：:Extracellular vesicles (EVs), such as exosomes, have garnered increasing interest because of their potential clinical applications that range from diagnostics to therapeutics. The development of an automated and reproducible EV purification platform would therefore aid the introduction of EV biomarkers and therapies i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75561-0
更新日期：2020-10-30 00:00:00
abstract：:Healthy neonates exhibit a well-functioning haemostatic system despite peculiarities regarding composition of clotting factors and inhibitors as well as impaired platelet aggregation. Thrombocytopenia and severe bleeding events are feared in sick infants. Recombinant factor VIIa (rFVIIa) is a haemostatic agent used as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44199-y
更新日期：2019-05-29 00:00:00
abstract：:Chronic myeloid leukemia (CML) is characterized in the majority of cases by a t(9;22)(q34;q11) translocation, also called the Philadelphia chromosome, giving rise to the BCR-ABL1 fusion protein. Current treatment with tyrosine kinase inhibitors is directed against the constitutively active ABL1 domain of the fusion pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00755-y
更新日期：2017-04-04 00:00:00
abstract：:Periocular skin warming reportedly improves the objective and subjective sleep quality in adults with mild difficulty in falling asleep. To clarify the effects of periocular warming, we examined the distal skin temperatures (hands and feet), proximal skin temperature (infraclavicular region) and core body temperature ...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-42116-x
更新日期：2019-04-05 00:00:00
abstract：:Refractive surgical treatment of hyperopia still remains a challenge for refractive surgeons. A new nomogram of small incision lenticule extraction (SMILE) procedure has recently been developed for the treatment of hyperopia. In the present study, we aimed to evaluate the wound healing and inflammatory responses of th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29802
更新日期：2016-07-15 00:00:00
abstract：:Sterile liver inflammation and fibrosis are associated with many liver disorders of different etiologies. Both type 1 and type 2 inflammatory responses have been reported to contribute to liver pathology. However, the mechanisms controlling the balance between these responses are largely unknown. Natural killer T (NKT...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78688-2
更新日期：2020-12-11 00:00:00
abstract：:Host-generalist pathogens sporadically infect naive hosts, potentially triggering epizootics. The waterborne fungus Batrachochytrium dendrobatidis (Bd) is linked to declines of hundreds of amphibian species with aquatic larvae. Although several population declines and extinctions attributed to Bd have been reported am...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16425-y
更新日期：2017-11-30 00:00:00
abstract：:Alterations in the composition of commensal bacterial populations, a phenomenon known as dysbiosis, are linked to multiple gastrointestinal disorders, such as inflammatory bowel disease and irritable bowel syndrome, or to infections by diverse enteric pathogens. Blastocystis is one of the most common single-celled euk...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25255
更新日期：2016-05-05 00:00:00
abstract：:The mechanism by which the HIV-1 MPER epitope is recognized by the potent neutralizing antibody 10E8 at membrane interfaces remains poorly understood. To solve this problem, we have optimized a 10E8 peptide epitope and analyzed the structure and binding activities of the antibody in membrane and membrane-like environm...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38177
更新日期：2016-12-01 00:00:00
abstract：:Pulmonary tuberculosis (PTB) results in lung functional impairment and there are no surrogate markers to monitor the extent of lung involvement. We investigated the clinical significance of S100A12 and soluble receptor for advanced glycation end-products (sRAGE) for predicting the extent of lung involvement. We perfor...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep31798
更新日期：2016-08-19 00:00:00
abstract：:The parasitic salmon louse, and its resistance to chemical delousing agents, represents one of the largest challenges to the salmon aquaculture industry. We genotyped lice sampled from wild salmon and sea trout throughout Norway with the recently identified Phe362Tyr mutation that conveys resistance to organophosphate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14681-6
更新日期：2017-10-27 00:00:00
abstract：:Self-organization and pattern formation in network-organized systems emerges from the collective activation and interaction of many interconnected units. A striking feature of these non-equilibrium structures is that they are often localized and robust: only a small subset of the nodes, or cell assembly, is activated....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21360
更新日期：2016-02-17 00:00:00
abstract：:In this study, the effects of different feeding frequencies on the growth and the expression of genes in the GH/IGF axis were assessed in juvenile Chinese sturgeon. The newly hatched Chinese sturgeons were bred for 38 days at three different feeding frequencies groups (feeding frequency of two times a day, TWD; three ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74120-x
更新日期：2020-10-15 00:00:00
abstract：:Hedgehog interacting protein (Hhip) is essential for islet formation and beta-cell proliferation during pancreatic development; abnormally elevated Hhip expression has been linked to human pancreatitis. Here, we investigate the role of Hhip in modulating insulin secretion in adult Hhip mice (Hhip +/- vs. Hhip+/+) fed ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47633-3
更新日期：2019-08-01 00:00:00
abstract：:MRI of hyperpolarized media, such as (129)Xe and (3)He, shows great potential for clinical applications. The optimal use of the available spin polarization requires accurate flip angle calibrations and T1 measurements. Traditional flip angle calibration methods are time-consuming and suffer from polarization losses du...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25854
更新日期：2016-05-12 00:00:00
abstract：:This study investigated the variation characteristics of micro-topography during successive erosive stages of water erosion: splash erosion (SpE), sheet erosion (ShE), and rill erosion (RE). Micro-topography was quantified using surface elevation change, soil roughness (SR) and multifractal model. Results showed that ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21852-6
更新日期：2018-02-22 00:00:00
abstract：:Despite its intrinsic advantages, translation of laser induced breakdown spectroscopy for material identification has been often impeded by the lack of robustness of developed classification models, often due to the presence of spurious correlations. While a number of classifiers exhibiting high discriminatory power h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13169
更新日期：2015-08-19 00:00:00
abstract：:This paper presents the design, simulation and low-cost fabrication of microfluidic channel for biomedical application. Channel is fabricated using soft lithography technique. Printed Circuit Board (PCB) is used to make the master for the channel. Channel pattern is transferred on PCB plate using toner transfer techni...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65995-x
更新日期：2020-06-08 00:00:00
abstract：:The growth in energy devices and the role of supercapacitors are increasingly important in today's world. Designing an electrode material for supercapacitors using metals that have high performance, superior structure, are eco-friendly, inexpensive and highly abundant is essentially required for commercialization. In ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75879-9
更新日期：2020-11-05 00:00:00
abstract：:We studied whether in patients with stable COPD blood gases (BG), especially oxygenated hemoglobin (OxyHem) as a novel biomarker confer information on disease burden and prognosis and how this adds to the information provided by the comorbidity pattern and systemic inflammation. Data from 2137 patients (GOLD grades 1-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67197-x
更新日期：2020-06-29 00:00:00
abstract：:Anti-PD-1 antibodies revolutionized the treatment of advanced melanoma patients. However, one out of three do not respond to this therapy, with an overall poor prognosis. Identification of predictive biomarkers in patients receiving immune-based therapies is necessary for minimizing risk of toxicity and optimizing pat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72711-2
更新日期：2020-10-19 00:00:00
abstract：:A new flash (ultra-rapid) spark plasma sintering method applicable to various materials systems, regardless of their electrical resistivity, is developed. A number of powders ranging from metals to electrically insulative ceramics have been successfully densified resulting in homogeneous microstructures within sinteri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15365-x
更新日期：2017-11-08 00:00:00
abstract：:Stem cell therapy has long been considered a promising mode of treatment for many incurable diseases. Human mesenchymal stem cells (hMSCs) have provided the most promising results to date for regenerative medicine. Nevertheless, due to several obstacles such as difficulty in sourcing and characterizing hMSCs, they rem...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42669-x
更新日期：2019-04-16 00:00:00
abstract：:Identifying how spatially distributed information becomes integrated in the brain is essential to understanding higher cognitive functions. Previous computational and empirical studies suggest a significant influence of brain network structure on brain network function. However, there have been few analytical approach...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46606
更新日期：2017-04-20 00:00:00
abstract：:Although pneumococcal infection is a serious problem worldwide and has a high mortality rate, the molecular mechanisms underlying the lethality caused by pneumococcus remain elusive. Here, we show that BLT2, a G protein-coupled receptor for leukotriene B4 and 12(S)-hydroxyheptadecatrienoic acid (12-HHT), protects mice...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34560
更新日期：2016-10-05 00:00:00
abstract：:Oestrogen is an important regulator in reproduction. To understand the role of oestrogen receptor 1 (ESR1) in Leydig cells, we investigated the expression of ESR1 in mouse Leydig cells during postnatal development and the effects of oestrogen on steroidogenesis and proliferation of progenitor Leydig cells (PLCs). In L...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43459
更新日期：2017-03-07 00:00:00
abstract：:Repeated social defeat stress (R-SDS) induces multiple behavioral changes in mice. However, the relationships between these behavioral changes were not fully understood. In the first experiment, to examine how the social avoidance is related to R-SDS-impaired behavioral flexibility, 10-week-old male C57BL/6N mice rece...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28803-1
更新日期：2018-07-11 00:00:00
abstract：:Acquisition of nutrients during intra-vacuolar growth of L. pneumophila within macrophages or amoebae is poorly understood. Since many genes of L. pneumophila are acquired by inter-kingdom horizontal gene transfer from eukaryotic hosts, we examined the presence of human solute carrier (SLC)-like transporters in the L....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26782-x
更新日期：2018-05-29 00:00:00
