Abstract：
:Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Schwarz J,Bierbaum V,Merrin J,Frank T,Hauschild R,Bollenbach T,Tay S,Sixt M,Mehling Mdoi
10.1038/srep36440subject
Has Abstractpub_date
2016-11-07 00:00:00pages
36440issn
2045-2322pii
srep36440journal_volume
6pub_type
杂志文章abstract：:Magneto-optical Faraday effect is widely applied in optical devices and is indispensable for optical communications and advanced information technology. However, the bismuth garnet Bi-YIG is only the Faraday material since 1972. Here we introduce (Fe, FeCo)-(Al-,Y-fluoride) nanogranular films exhibiting giant Faraday ...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-03-21 00:00:00
abstract：:Quantum key distribution involving decoy-states is a significant application of quantum information. By using three-intensity decoy-states of single-photon-added coherent sources, we propose a practically realizable scheme on quantum key distribution which approaches very closely the ideal asymptotic case of an infini...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15130
更新日期：2015-10-14 00:00:00
abstract：:Bacterial artificial chromosomes (BACs) offer a means of manipulating gene expression and tagging gene products in the mammalian genome without the need to alter endogenous gene structure and risk deleterious phenotypic consequences. However, for a BAC clone to be useful for such purposes it must be shown to contain a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35458-5
更新日期：2018-11-23 00:00:00
abstract：:The activation and regulation of coagulation Factor XIII (FXIII) protein has been the subject of active research for the past three decades. Although discrete evidence exists on various aspects of FXIII activation and regulation a combinatorial structure/functional view in this regard is lacking. In this study, we pre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30105
更新日期：2016-07-25 00:00:00
abstract：:Aging is accompanied by a decline in memory and other brain functions. Physical exercise may mitigate this decline through the modulation of factors participating in the crosstalk between skeletal muscle and the brain, such as neurotrophins and oxidative stress parameters. We aimed to determine whether long term exerc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40040-8
更新日期：2019-03-04 00:00:00
abstract：:We developed hybrid slot antenna structures for microbial sensing in the THz frequency range, where silver nanowires (AgNWs) were employed to increase the sensitivity. In order to fabricate the hybrid devices, we partially etched the AgNW in the slot antenna region, where we can expect the field enhancement effect at ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33617-2
更新日期：2018-10-19 00:00:00
abstract：:A central challenge in designing and administering effective anticoagulants is achieving the proper therapeutic window and dosage for each patient. The Hill coefficient, nH, which measures the steepness of a dose-response relationship, may be a useful gauge of this therapeutic window. We sought to measure the Hill coe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29387
更新日期：2016-07-21 00:00:00
abstract：:Specialized processing in the brain is performed by multiple groups of brain regions organized as functional modules. Although, in vivo studies of brain functional modules involve multiple functional Magnetic Resonance Imaging (fMRI) scans, the methods used to derive functional modules from functional networks of the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63552-0
更新日期：2020-05-05 00:00:00
abstract：:Accurate alignment of an intraocular lens (IOL) is indispensable for achieving accurate postoperative refractive outcomes. Thus, we evaluated decentration and tilt of single- and three-piece IOLs, as well as anterior chamber depth (ACD), at 3 hours, 24 hours, 2 weeks, and 4 weeks after cataract surgery, using swept-so...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
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更新日期：2018-07-06 00:00:00
abstract：:The Fabry-Pérot interferometer is used in a variety of high-precision optical interferometry applications, such as gravitational wave detection. It is also used in various types of laser resonators to act as a narrow band filter. In addition, ultra-compact Fabry-Pérot interferometers are used in the optical resonators...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-10-12 00:00:00
abstract：:Epidemiological investigation have suggested that there is a significantly inverse association between circulating 25-hydroxyvitamin D (25(OH)D) and the risk for developing colorectal cancer (CRC) in humans. However, little is known about the role of vitamin D binding protein (VDBP) in colorectal carcinogenesis. Blood...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-01-22 00:00:00
abstract：:Nanospheres of lead (Pb) have recently been identified in zircon (ZrSiO4) with the potential to compromise the veracity of U-Pb age determinations. The key assumption that the determined age is robust against the effects of Pb mobility, as long as Pb is not lost from the zircon during subsequent geological events, is ...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-09-23 00:00:00
abstract：:RNA virus-based episomal vector (REVec) is an emerging viral vector system that mediates long-term stable gene expression in variety of cell types in vitro. However, little is known about its tissue tropism and persistence of gene expression in vivo. Here, to evaluate the feasibility of REVec for in vivo gene delivery...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62630-7
更新日期：2020-04-03 00:00:00
abstract：:Urbanization-driven landscape changes are harmful to many species. Negative effects can be mitigated through habitat preservation and restoration, but it is often difficult to prioritize these conservation actions. This is due, in part, to the scarcity of species response data, which limit the predictive accuracy of m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79258-2
更新日期：2021-01-12 00:00:00
abstract：:Lung cancer is the leading cause of death among all malignancies due to rapid tumor progression and relapse; however, the underlying molecular mechanisms of tumor progression are unclear. In the present study, we identified ANKRD22 as a novel tumor-associated gene in non-small cell lung cancer (NSCLC). According to th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04818-y
更新日期：2017-06-30 00:00:00
abstract：:Plant extracts contain many active compounds, which are tremendously fruitful for plant defence against several insect pests. The prime objectives of the present study were to calculate the extraction yield and to evaluate the leaf extracts of Citrullus colocynthis (L.), Cannabis indica (L.) and Artemisia argyi (L.) a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57092-5
更新日期：2020-01-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,收录出版
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更新日期：2019-10-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09518
更新日期：2015-04-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-03-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-03-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-02-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-08-03 00:00:00
abstract：:Archaeologically defined Upper Palaeolithic (UP, 45,000-10,000 years ago) "cultures" are often used as proxies to designate fossil populations. While recent genomic studies have partly clarified the complex relationship between European UP "cultures" and past population dynamics, they leave open numerous questions reg...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-12-14 00:00:00
abstract：:Mammalian parental genomes contribute differently to early embryonic development. Before activation of the zygotic genome, the maternal genome provides all transcripts and proteins required for the transition from a highly specialized oocyte to a pluripotent embryo. Depletion of these maternally-encoded transcripts fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44667
更新日期：2017-03-20 00:00:00
abstract：:Transient receptor potential melastatin 8 (TRPM8), a nonselective cation channel, is the predominant mammalian cold temperature thermosensor and it is activated by cold temperatures and cooling compounds, such as menthol and icilin. Because of its role in cold allodynia, cold hyperalgesia and painful syndromes TRPM8 a...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-09-08 00:00:00
abstract：:For infants with acute progressive hydrocephalus, invasive drainage of cerebrospinal fluid (CSF) is performed until a ventriculo-peritoneal shunt can be inserted. Surrogate markers of intracranial pressure (ICP) may help optimise the timing of invasive procedures. To assess whether RI with/without fontanel compression...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30274-3
更新日期：2018-08-07 00:00:00
abstract：:Obesity affects male fertility and maternal diabetes affects the offspring sperm epigenome. However, the effects of in utero exposure to maternal glucose intolerance in combination with postnatal high fat, high sucrose (HFHS) diet consumption on offspring spermatogenesis is not clear. The present study was designed to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25950-3
更新日期：2018-05-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20241-3
更新日期：2018-02-01 00:00:00
abstract：:The modern Asian monsoonal systems are currently believed to have originated around the end of the Oligocene following a crucial step of uplift of the Tibetan-Himalayan highlands. Although monsoon possibly drove the evolution of many mammal lineages during the Neogene, no evidence thereof has been provided so far. We ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09008
更新日期：2015-03-11 00:00:00
abstract：:Frailty is defined as a decline in an organism's physiological reserves resulting in increased vulnerability to stressors. In humans, a single continuous variable, the so-called Frailty Index (FI), can be obtained by multidimensionally assessing the biological complexity of an ageing organism. Here, we evaluate this v...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52585-9
更新日期：2019-11-14 00:00:00
