Abstract:
OBJECTIVE:The aim of this study was to analyze the biological effects of MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells (hPDLSCs) after exposure to acidic and neutral environments. MATERIALS AND METHODS:Discs of each material (n = 30) were exposed to phosphate buffered saline (pH = 7.4) or butyric acid (pH = 5.2) for 7 days, and biological testing was carried out in vitro on hPDLSCs. Cell viability and apoptosis assays were performed using eluates of each root-end filling material. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the root-end filling materials was determined by energy-dispersive x-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by ANOVA and Tukey test (p < 0.05). RESULTS:Under an acidic environment, both materials displayed similar ion release abilities, with the increased release of Si and Ca ions. Substantial changes in microstructure were observed for both materials after exposure to acidic pH. In addition, material exposure to an acidic environment showed a similar degree of cell adherence, and, surprisingly, MTA Repair HP exhibited higher cell viability rates at pH 5.2 than ProRoot MTA. CONCLUSIONS:Exposure to an acidic environment promoted Si and Ca ion release from ProRoot MTA and MTA Repair HP. Moreover, we observed optimal biological properties of ProRoot MTA and MTA Repair HP in terms of cell viability, cell death, and cell attachment in both environments. CLINICAL RELEVANCE:These results may suggest that MTA Repair HP and ProRoot exhibited optimal biological properties in terms of cell viability, cell death and cell attachment in acidic environment, being considered as materials for root-end filling and perforations.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Collado-González M,López-García S,García-Bernal D,Oñate-Sánchez RE,Tomás-Catalá CJ,Moraleda JM,Lozano A,Forner L,Rodríguez-Lozano FJdoi
10.1007/s00784-019-02822-2subject
Has Abstractpub_date
2019-10-01 00:00:00pages
3915-3924issue
10eissn
1432-6981issn
1436-3771pii
10.1007/s00784-019-02822-2journal_volume
23pub_type
杂志文章abstract:BACKGROUND:Few studies regarding the distribution of pediatric oral diseases are available. Thus, the aim of this study was to investigate the incidence and demographic profile of neoplasms and non-neoplastic lesions among children and adolescents (0-19 years old). METHODS:A retrospective descriptive cross-sectional s...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2581-0
更新日期:2019-04-01 00:00:00
abstract::This study compared shear bond strengths of six self-adhesive resin cements to zirconium oxide ceramic with and without air-particle abrasion. One hundred twenty zirconia samples were air-abraded (group SB; n = 60) or left untreated (group NO). Composite cylinders were bonded to the zirconia samples with either BisCem...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0278-0
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVES:To assess dimensional changes following alveolar ridge preservation using bovine-derived xenograft with 10% collagen and collagen membrane compared to ridge preservation by means of bovine-derived xenograft particles and collagen membrane or spontaneous healing in posterior sites. MATERIALS AND METHODS:Fort...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-019-02979-w
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:Information about the spectrum of microorganisms in the intraimplant cavities of two-piece dental implants is scarce. The purpose of this study was to assess the intraimplant microflora of two-piece dental implants by conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0805-2
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVES:The aims of this study were to determine the quality of life of periodontally compromised patients after implant treatment (primary aim) and their satisfaction with the restoration and treatment approach (secondary aim). MATERIAL AND METHODS:In this study, 61 adult subjects were evaluated following non-surg...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1561-x
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:Aggregatibacter actinomycetemcomitans, specially its highly leucotoxic strain (JP2 clone), represents an etiological factor for the onset and progression of aggressive types of periodontitis. The aims of this investigation were to investigate the most relevant periodontal pathogens in the subgingival microbio...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1653-7
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:C-shaped root canal configuration is an unusual root canal morphology and one of the most often anatomical variations in root canal systems of second mandibular molars. The purpose of this study was to assess the prevalence, symmetry and configuration of C-shaped canals in first and second mandibular molars ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1993-y
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:Reconstruction of large and complex bone segments is a challenging problem facing maxillofacial surgery. The majority of current regenerative approaches rely on extrinsic vascularization, which is deficient after cancer ablation and irradiation. The aim of the study was to investigate the efficacy of intrinsi...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1143-8
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the effects of periodontal treatment on the inflammatory markers in gingival crevicular fluid and the concentration of salivary cortisol between non-pregnant and pregnant women with gingivitis. MATERIALS AND METHODS:This study included 30 non-pregnant women (mean age 27....
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2569-9
更新日期:2018-07-31 00:00:00
abstract:: ...
journal_title:Clinical oral investigations
pub_type: 评论,信件
doi:10.1007/s00784-018-2788-0
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:This work aimed to comparatively evaluate the three modalities of vestibuloplasty around implants in the posterior mandible. MATERIALS AND METHODS:Forty patients who presented with a shallow vestibule and insufficient keratinized gingiva were divided into three groups: apically positioned flap only (APF gro...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2320-y
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the association between salivary and plaque arginine levels/ADS activities with dental caries. MATERIALS AND METHODS:A systematic search was performed as per PRISMA statement using PubMed, Scopus, Cochrane Library, and Web of Science. Published studies that invest...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00784-017-2322-9
更新日期:2018-03-01 00:00:00
abstract::This study evaluated the colour parameters of composite and ceramic shade guides determined using a colorimeter and digital imaging method with illuminants at different colour temperatures. Two different resin composite shade guides, namely Charisma (Heraeus Kulzer) and Premise (Kerr Corporation), and two different ce...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0329-6
更新日期:2010-12-01 00:00:00
abstract::The aim of this study was to investigate the different variables in mesio-vertically positioned impacted or erupted third molars in a group of volunteers in Turkey to determine the angular changes and levels of eruption radiographically using standardised panoramic radiographs. There were 213 volunteers with impacted ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-001-0144-1
更新日期:2002-06-01 00:00:00
abstract::The aim of the study was to monitor changes in oral health and oral biofilm composition in vivo during an experiment simulating prehistoric lifestyle and diet and poor oral hygiene. Thirteen subjects lived for a period of 8 weeks under Neolithic conditions. The following clinical parameters were recorded before and af...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0318-9
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVES:This systematic review (SR) aimed to summarise and critically appraise available evidence about the association between the synergistic consumption of alcohol and tobacco and the occurrence of oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS:Observational studies investigating the association betw...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-019-02958-1
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP). MATERIALS AND METHODS:We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxill...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1166-1
更新日期:2014-11-01 00:00:00
abstract::The aim of the study was the morphological analysis of coronal dentin caries and the modifications induced by different pretreatments with phosphoric acid or sodium hypochlorite. Carious dentin specimens were obtained from human molars affected by carious lesions. Specimens were divided in four groups and submitted to...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00784-003-0221-8
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to assess the effects of three different fluoride-releasing agents on preventing white spot lesions that occur during treatment with full coverage rapid maxillary expanders. MATERIALS AND METHODS:A total of 48 patients with transverse maxillary deficiency were randomly assigned to f...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2749-7
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:The aim of this retrospective cephalometric study was to compare treatment outcomes with "bite jumping appliance" (BJA) or Andresen-Häupl type activator. It especially focused on skeletal and dental structures in patients with class II malocclusion. The study hypothesis was that differences in treatment-rela...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03115-4
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Candida albicans is a common fungal infection and is commensal in 40-65 % of healthy adults. The development and pharmacokinetics of a novel sustained release clotrimazole varnish (Clot-SRV) for topical oral use have been reported. The aim of this study was to compare the efficacy of this varnish with clotri...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1259-5
更新日期:2015-03-01 00:00:00
abstract::The aim of this study was to evaluate the clinical long-term performance of a visible light-cured resin (VLCR) denture base material and to compare it to a well-established polymethylmethacrylate (PMMA)-based denture acrylic in a randomized split-mouth clinical long-term study. One hundred removable partial dentures i...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-011-0572-5
更新日期:2012-06-01 00:00:00
abstract::Aims of the study were (1) to establish a method for quantification of chlorhexidine (CHX) in small volumes and (2) to determine CHX release from differently concentrated CHX-containing preparations, varnishes, and a CHX gel applied on artificial fissures. CHX determination was conducted in a microplate reader using p...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-007-0166-4
更新日期:2008-09-01 00:00:00
abstract::The aim of the current study was to evaluate the prevalence of psychopathological symptoms in patients who self-reported different forms of bruxism by means of clinical and anamnestic diagnostic criteria. Eighty-five participants were divided into four groups as sleep bruxers (12), awake bruxers (24), sleep-awake brux...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-010-0492-9
更新日期:2012-02-01 00:00:00
abstract::The present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (PCR). Twenty patients with untreated chronic periodontitis were randomized into two groups. Before subgingival instrumentati...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0333-x
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:Intrapapillary injection (IPI) has been suggested to improve pulpal anesthesia of mandibular teeth and to avoid complications from inferior alveolar nerve block (IANB). This study aimed to determine and compare clinical efficacies and prostaglandin E2 (PGE2) levels between IPI and IANB. MATERIALS AND METHODS...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-019-02954-5
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was the qualitative and quantitative evaluation of osseous graft consolidation using allogeneic bone blocks for vertical bone augmentation in an animal model. MATERIAL AND METHODS:Standardised allogeneic and autologous bone blocks were fixed on the frontal skull of 20 adult fema...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03151-0
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:An optimized oral health-related section and a video training were developed and validated for the interRAI suite of instruments. The latter is completed by professional non-dental caregivers and used in more than 40 countries to assess care needs of older adults. METHODS:The optimized oral health-related s...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03669-8
更新日期:2020-11-16 00:00:00
abstract:OBJECTIVES:Aberrant growth of the maxillomandibular complex in patients with cleft lip and/or palate (CL/P) can be managed with dentofacial orthopaedics. However, no consensus has been reached regarding timing and evolution of the maturational stages. Therefore, the aim of this study is to determine if patients with CL...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-021-03790-2
更新日期:2021-01-23 00:00:00
abstract:OBJECTIVES:We aimed to determine the immunolocalization patterns of the interleukin (IL)-6 signaling complex in epithelialized and non-epithelialized apical lesions of endodontic origin (ALEOs). MATERIALS AND METHODS:Epithelialized (n = 8) and non-epithelialized (n = 7) ALEOs were obtained from teeth with indication o...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03738-y
更新日期:2021-01-07 00:00:00