Abstract:
:Optogenetics enables manipulation of biological processes with light at high spatio-temporal resolution to control the behavior of cells, networks, or even whole animals. In contrast to the performance of excitatory rhodopsins, the effectiveness of inhibitory optogenetic tools is still insufficient. Here we report a two-component optical silencer system comprising photoactivated adenylyl cyclases (PACs) and the small cyclic nucleotide-gated potassium channel SthK. Activation of this 'PAC-K' silencer by brief pulses of low-intensity blue light causes robust and reversible silencing of cardiomyocyte excitation and neuronal firing. In vivo expression of PAC-K in mouse and zebrafish neurons is well tolerated, where blue light inhibits neuronal activity and blocks motor responses. In combination with red-light absorbing channelrhodopsins, the distinct action spectra of PACs allow independent bimodal control of neuronal activity. PAC-K represents a reliable optogenetic silencer with intrinsic amplification for sustained potassium-mediated hyperpolarization, conferring high operational light sensitivity to the cells of interest.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Bernal Sierra YA,Rost BR,Pofahl M,Fernandes AM,Kopton RA,Moser S,Holtkamp D,Masala N,Beed P,Tukker JJ,Oldani S,Bönigk W,Kohl P,Baier H,Schneider-Warme F,Hegemann P,Beck H,Seifert R,Schmitz Ddoi
10.1038/s41467-018-07038-8subject
Has Abstractpub_date
2018-11-05 00:00:00pages
4611issue
1issn
2041-1723pii
10.1038/s41467-018-07038-8journal_volume
9pub_type
杂志文章abstract::Generation and recombination of electrons and holes in organic solar cells occurs via charge transfer states located at the donor/acceptor interface. The energy of these charge transfer states is a crucial factor for the attainable open-circuit voltage and its correct determination is thus of utmost importance for a d...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05987-8
更新日期:2018-09-07 00:00:00
abstract::The causes of impaired skeletal muscle mass and strength during aging are well-studied in healthy populations. Less is known on pathological age-related muscle wasting and weakness termed sarcopenia, which directly impacts physical autonomy and survival. Here, we compare genome-wide transcriptional changes of sarcopen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13694-1
更新日期:2019-12-20 00:00:00
abstract::Homologous recombination-mediated gene targeting has greatly contributed to genetic analysis in a wide range of species, but is highly inefficient in human cells because of overwhelmingly frequent random integration events, whose molecular mechanism remains elusive. Here we show that DNA polymerase θ, despite its mino...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms16112
更新日期:2017-07-11 00:00:00
abstract::While intron retention (IR) is considered a widely conserved and distinct mechanism of gene expression control, its regulation is poorly understood. Here we show that DNA methylation directly regulates IR. We also find reduced occupancy of MeCP2 near the splice junctions of retained introns, mirroring the reduced DNA ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15134
更新日期:2017-05-08 00:00:00
abstract::Despite the lack of a layered neocortex and fundamental differences in endbrain organization in birds compared with mammals, intelligent species evolved from both vertebrate classes. Among birds, corvids show exceptional cognitive flexibility. Here we explore the neuronal foundation of corvid cognition by recording si...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3878
更新日期:2013-01-01 00:00:00
abstract::Marine particles transport organic matter through the water column to the sediment where the organic matter can be buried. This pathway is one of the few natural removal mechanisms of CO2 from the atmosphere over geological time. Picophytoplankton, major primary producers in the ocean, have until recently been thought...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5143
更新日期:2014-06-12 00:00:00
abstract::Primary effusion lymphoma (PEL) is caused by Kaposi's sarcoma-associated herpesvirus. Our understanding of PEL is poor and therefore treatment strategies are lacking. To address this need, we conducted genome-wide CRISPR/Cas9 knockout screens in eight PEL cell lines. Integration with data from unrelated cancers identi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05506-9
更新日期:2018-08-15 00:00:00
abstract::Pulses of tree mortality caused by drought have been reported recently in forests around the globe, but large-scale quantitative evidence is lacking for Europe. Analyzing high-resolution annual satellite-based canopy mortality maps from 1987 to 2016 we here show that excess forest mortality (i.e., canopy mortality exc...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19924-1
更新日期:2020-12-03 00:00:00
abstract::Observational studies have reported inconsistent associations between circulating lipids and breast cancer risk. Using results from >400,000 participants in two-sample Mendelian randomization, we show that genetically raised LDL-cholesterol is associated with higher risk of breast cancer (odds ratio, OR, per standard ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06467-9
更新日期:2018-09-27 00:00:00
abstract::FR171456 is a natural product with cholesterol-lowering properties in animal models, but its molecular target is unknown, which hinders further drug development. Here we show that FR171456 specifically targets the sterol-4-alpha-carboxylate-3-dehydrogenase (Saccharomyces cerevisiae--Erg26p, Homo sapiens--NSDHL (NAD(P)...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9613
更新日期:2015-10-12 00:00:00
abstract::No method exists to measure large-scale translation of genes in uncultured organisms in microbiomes. To overcome this limitation, we develop MetaRibo-Seq, a method for simultaneous ribosome profiling of tens to hundreds of organisms in microbiome samples. MetaRibo-Seq was benchmarked against gold-standard Ribo-Seq in ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17081-z
更新日期:2020-06-29 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-019-11205-w
更新日期:2019-07-12 00:00:00
abstract::The Dead Sea is one of the most hypersaline habitats on Earth. The fungus Eurotium rubrum (Eurotiomycetes) is among the few species able to survive there. Here we highlight its adaptive strategies, based on genome analysis and transcriptome profiling. The 26.2 Mb genome of E. rubrum shows, for example, gains in gene f...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4745
更新日期:2014-05-09 00:00:00
abstract::Postsynaptic density (PSD) proteins have been implicated in the pathophysiology of neurodevelopmental and psychiatric disorders. Here, we present detailed clinical and genetic data for 20 patients with likely gene-disrupting mutations in TANC2-whose protein product interacts with multiple PSD proteins. Pediatric patie...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12435-8
更新日期:2019-10-15 00:00:00
abstract::Optical super-resolution microscopy allows nanoscale imaging of protein molecules in intact biological tissues. However, it is still challenging to perform large volume super-resolution imaging for entire animal organs. Here we develop a single-wavelength Bessel lightsheet method, optimized for refractive-index matchi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12715-3
更新日期:2019-10-18 00:00:00
abstract::Proton conduction is a fundamental process in biology and in devices such as proton exchange membrane fuel cells. To maximize proton conduction, three-dimensional conduction pathways are preferred over one-dimensional pathways, which prevent conduction in two dimensions. Many crystalline porous solids to date show one...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12750
更新日期:2016-09-13 00:00:00
abstract::Biogenic furans are currently discussed as highly attractive alternative feedstock in a post-fossil society; thus, also the creation of sustainable furan valorization pathways appears of great importance. Here an artificial Achmatowicz monooxygenase activity for the aerobic ring expansion of furans is achieved by the ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6278
更新日期:2014-10-22 00:00:00
abstract::Considerable interest has been devoted to converting mechanical energy into electricity using polymer nanofibres. In particular, piezoelectric nanofibres produced by electrospinning have shown remarkable mechanical energy-to-electricity conversion ability. However, there is little data for the acoustic-to-electric con...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11108
更新日期:2016-03-23 00:00:00
abstract::In the bottom-up synthesis of graphene nanoribbons (GNRs) from self-assembled linear polymer intermediates, surface-assisted cyclodehydrogenations usually take place on catalytic metal surfaces. Here we demonstrate the formation of GNRs from quasi-freestanding polymers assisted by hole injections from a scanning tunne...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14815
更新日期:2017-03-13 00:00:00
abstract::Ever since the discovery of dye self-assemblies in nature, there have been tremendous efforts to exploit biomimetic supramolecular assemblies for tailored artificial photon processing materials. This feature necessarily has resulted in an increasing demand for understanding exciton dynamics in the dye self-assemblies....
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9646
更新日期:2015-10-23 00:00:00
abstract::In human cancers, oncogenic mutations commonly occur in the RAS genes KRAS, NRAS, or HRAS, but there are no clinical RAS inhibitors. Mutations are more prevalent in KRAS, possibly suggesting a unique oncogenic activity mediated by KRAS-specific interaction partners, which might be targeted. Here, we determine the spec...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05692-6
更新日期:2018-09-07 00:00:00
abstract::Somatic mutations in the calreticulin (CALR) gene are associated with approximately 30% of essential thrombocythemia (ET) and primary myelofibrosis (PMF). CALR mutations, including the two most frequent 52 bp deletion (del52) and 5 bp insertion (ins5), induce a frameshift to the same alternative reading frame generati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18691-3
更新日期:2020-09-28 00:00:00
abstract::Crystal structures of G protein-coupled receptor (GPCR) ligand complexes allow a rational design of novel molecular probes and drugs. Here we report the structure-guided design, chemical synthesis and biological investigations of bivalent ligands for dopamine D2 receptor/neurotensin NTS1 receptor (D2R/NTS1R) heterodim...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12298
更新日期:2016-07-26 00:00:00
abstract::Metagenomic sequencing provides a window into microbial community structure and metabolic potential; however, linking these data to exogenous metabolites that microorganisms process and produce (the exometabolome) remains challenging. Previously, we observed strong exometabolite niche partitioning among bacterial isol...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02356-9
更新日期:2018-01-02 00:00:00
abstract::During plant radial growth typically seen in trees, procambial and cambial cells act as meristematic cells in the vascular system to self-proliferate and differentiate into xylem cells. These two processes are regulated by a signalling pathway composed of a peptide ligand and its receptor; tracheary element differenti...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4504
更新日期:2014-03-24 00:00:00
abstract::Suspension-feeding fishes such as goldfish and whale sharks retain prey without clogging their oral filters, whereas clogging is a major expense in industrial crossflow filtration of beer, dairy foods and biotechnology products. Fishes' abilities to retain particles that are smaller than the pore size of the gill-rake...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11092
更新日期:2016-03-29 00:00:00
abstract::Mechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, osmoregulation, and morphogenesis. The mechanosensitive channels of small conductance (MscS) constitute a remarkably diverse su...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17538-1
更新日期:2020-07-23 00:00:00
abstract::Historical records and genetic analyses indicate that Latin Americans trace their ancestry mainly to the intermixing (admixture) of Native Americans, Europeans and Sub-Saharan Africans. Using novel haplotype-based methods, here we infer sub-continental ancestry in over 6,500 Latin Americans and evaluate the impact of ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07748-z
更新日期:2018-12-19 00:00:00
abstract::Nitrate is both an important nutrient and a signalling molecule for plants. Although several components of the nitrate signalling pathway have been identified, their hierarchical organization remains unclear. Here we show that the localization of NLP7, a member of the RWP-RK transcription factor family, is regulated b...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2650
更新日期:2013-01-01 00:00:00
abstract::The Cdc45-MCM-GINS (CMG) helicase unwinds DNA during the elongation step of eukaryotic genome duplication and this process depends on the MCM ATPase function. Whether CMG translocation occurs on single- or double-stranded DNA and how ATP hydrolysis drives DNA unwinding remain open questions. Here we use cryo-electron ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10708
更新日期:2016-02-18 00:00:00