Abstract：
:Tumor microenvironment (TME) is the cellular environment in which tumor exists, and it contributes to tumor formation and progression. The TME is composed of tumor cells, stromal cells, cytokines, and chemotactic factors of which fibroblasts are the main cellular components. In our present study, we found that colorectal cancer (CRC) cells expressing integrin αvβ6 clearly could induce morphological changes in inactive fibroblasts and increased the expression of activated fibroblast markers such as α-smooth muscle actin (α-SMA) and fibroblast-activating protein (FAP). Those activated fibroblasts in the TME are called cancer-associated fibroblasts (CAFs). In order to investigate the mechanism by which CRC cells expressing integrin αvβ6 activated CAFs, a series of assays have been carried out in the follow-up. We found that CRC cells could secrete inactive transforming growth factor β (TGF-β); however, integrin αvβ6 activated TGF-β, which subsequently activated fibroblasts. This process was disrupted by knockdown of integrin αvβ6. In contrast, activated fibroblasts could promote CRC cell invasion. In particular, the strengthening effect on expression of integrin αvβ6 in colon cancer cells was obvious. Additionally, we found that CAFs could secrete stromal cell-derived factor-1 (SDF-1) and promote CRC cell metastasis in distant organs via the SDF-1/C-X-C chemokine receptor type 4 (CXCR4) axis. Taken together, we assumed that CRC cells and CAFs activated one another and worked together to promote cancer progression, with integrin αvβ6 playing a role in the bi-directional regulation of these cells. Hence, integrin αvβ6 may serve as a therapeutic target for the future CRC treatment.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Peng C,Zou X,Xia W,Gao H,Li Z,Liu N,Xu Z,Gao C,He Z,Niu W,Fang R,Biswas S,Agrez M,Zhi X,Niu Jdoi
10.1042/BSR20180243subject
Has Abstractpub_date
2018-12-07 00:00:00issue
6eissn
0144-8463issn
1573-4935pii
BSR20180243journal_volume
38pub_type
杂志文章abstract：:Ric c1, an allergenic protein from Ricinus communis , is an insect α-amylase inhibitor that has become an occupational allergen. Ric c1 can cross-react with allergens from wheat, soybean, peanut, shrimp, fish, gluten, house dust, tobacco, and air fungus, thereby amplifying the concern and risks caused by Ricinus aller...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20171245
更新日期：2018-02-14 00:00:00
abstract：OBJECTIVE:The current study aimed to investigate the functional roles and clinical significance of microRNA-148a (miR-148a) in the progression of oral squamous cell carcinoma (OSCC). METHODS:Relative expression of miR-148a in OSCC cells and tissues were detected using quantitative real-time polymerase chain reaction (...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20182458
更新日期：2020-04-30 00:00:00
abstract：:Extracts from uterine cervical and body cancers, but not from benign tumor or intact tissues tested, were found to contain a growth-promoting activity which induced the proliferation of human endometrial fibroblasts. Exposure of cultured fibroblasts to the cancer extracts increased the rate of [3H]thymidine incorporat...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116850
更新日期：1990-02-01 00:00:00
abstract：: Background/aims: Studies have demonstrated that osteopontin (OPN) was associated with the severity and development of knee osteoarthritis (OA). Methods: The purpose of this case-control study was to investigate the association ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20182023
更新日期：2019-02-27 00:00:00
abstract：:Myelin Basic Protein, one of the major membrane protein component of the central nervous system, was used to probe the molecular mechanism of cellular activation by phytohaemagglutinin. Pre-treatment of human lymphocytes with myelin basic protein results in a lower rising of cytosolic concentration of free calcium aft...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116851
更新日期：1990-02-01 00:00:00
abstract：:Esophageal cancer is seen with increasing incidence, but the underlying mechanism of esophageal cancer is still unknown. Transient receptor potential melastatin (TRPM) is non-selective cation channels. It has been verified that TRPM channels play crucial roles in development and progression of multiple tumors. Increas...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20191878
更新日期：2019-10-30 00:00:00
abstract：:Chagas disease remains a serious health problem for countries where the most common mode of transmission is infection contracted from the feces of a Triatominae insect vector. In México, 32 species of Triatoma have been identified; amongst them, Triatoma (Meccus) pallidipennis is an endemic species reported to have hi...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180988
更新日期：2018-09-28 00:00:00
abstract：:Amphiphilic anionic peptides have been used to enhance the efficiency of transfection by helping plasmids to escape from endosomes to the cytosol. It has been shown that efficiency of an eicosamers containing five glutamyl residues (E5), can be considerably enhanced either by transforming it into a dimer or by adding ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1010382001654
更新日期：2000-10-01 00:00:00
abstract：:The molecular mechanism of the occurrence and development of papillary thyroid carcinoma (PTC) has been widely explored, but has not been completely elucidated. The present study aimed to identify and analyze genes associated with PTC by bioinformatics methods. Two independent datasets were downloaded from Gene Expres...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190083
更新日期：2019-04-02 00:00:00
abstract：BACKGROUND:Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas. METHODS:Quantitative ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200767
更新日期：2021-01-04 00:00:00
abstract：:High-affinity binding of 3H-folate in Triton X-100 solubilized membranes of human liver displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. Ultrogel AcA 44 chromatography of solubilized membranes saturated with 3H-folate revealed a major peak of 100 kDa and a ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01182482
更新日期：1991-06-01 00:00:00
abstract：:Acidic airway microenvironment is one of the representative pathophysiological features of chronic inflammatory respiratory diseases. Epithelial barrier function is maintained by TJs (tight junctions), which act as the first physical barrier against the inhaled substances and pathogens of airway. As previous studies d...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20130087
更新日期：2013-10-31 00:00:00
abstract：OBJECTIVE:To observe the restraining effect of IL-38 on inflammatory response in collagen-induced arthritis rats (CIA), and to explore the regulatory mechanism of SIRT1/HIF-1α signaling pathway. METHODS:40 SD rats were randomly divided into Control group, CIA group, CLL group and CLH group, with 10 rats in each group;...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20182431
更新日期：2020-05-29 00:00:00
abstract：:LncRNA PACER is a chondrocyte inflammation-associated long non-coding RNA (lncRNA), and chondrocyte inflammation is involved in osteoarthritis (OA). We observed that plasma PACER was down-regulated, while plasma HOTAIR was up-regulated in OA patients. Altered plasma levels of PACER and HOTAIR distinguished OA patients...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190404
更新日期：2019-06-07 00:00:00
abstract：:We aimed to confirm the role of miR-1296-5p in gastric cancer and to identify its target genes. The expression of miR-1296-5p was measured in gastric cancer tissues and cell lines. The function of miR-1296-5p was examined by the overexpression and inhibition of its expression in typical gastric cell lines as well as S...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181556
更新日期：2019-01-08 00:00:00
abstract：:Poly(A)+RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322. Immunological screening of 1,500 colonies with an affinity-purified antiserum against the uncoupling protein yielded five positive clones, pUCPr...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01145183
更新日期：1986-01-01 00:00:00
abstract：:The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01115252
更新日期：1982-07-01 00:00:00
abstract：:Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reporte...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20192061
更新日期：2020-01-31 00:00:00
abstract：:Whole-genome analyses become more and more necessary for pharmaceutical research. DNA chip hybridizations are an important tool for monitoring gene expression profiles during diseases or medical treatment. However, drug target identification and validation as well as an increasing number of antibodies and other polype...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1007/s10540-005-2848-y
更新日期：2005-02-01 00:00:00
abstract：:In India, Sorghum plant allergenicity was reported to be approximately 54.9%. Sorghum bicolor Polcalcin (Sorb PC) was identified as the panallergen but the specificity of this allergen is yet to be characterized. The present study was aimed to characterize the antigenic determinants of Sorb PC that are responsible for...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20191835
更新日期：2019-11-29 00:00:00
abstract：:Clustered Regularly Interspaced Short Palindromic Repeat activation-synergistic activation mediator system (CRISPRa-SAM) has been efficiently used to up-regulate the targeted genes in human and mouse. But it is not known whether the CRISPRa-SAM system can be used against porcine disease because its two important trans...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20191496
更新日期：2019-08-19 00:00:00
abstract：:Conflicting opinions were recently expressed concerning the possible effect of alpha 2-adrenergic agonists upon cyclic AMP production in pancreatic islets. In the present study, clonidine inhibited glucose-induced insulin release from rat pancreatic islets, this inhibitory effect being abolished by idazoxan. Clonidine...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122227
更新日期：1984-06-01 00:00:00
abstract：:Type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD) is difficult to treat. The present study explored the efficacy of (liraglutide) Lira in treating T2DM complicated with NAFLD. A total of 127 patients suffering from T2DM complicated with NAFLD were enrolled in the present study,...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181304
更新日期：2018-12-21 00:00:00
abstract：:We have shown previously that Escherichia coli K92 produces two different capsular polymers known as CA (colanic acid) and PA (polysialic acid) in a thermoregulated manner. The complex Rcs phosphorelay is largely related to the regulation of CA synthesis. Through deletion of rscA and rscB genes, we show that the Rcs s...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20130018
更新日期：2013-05-24 00:00:00
abstract：:Recent experimental evidence increasingly shows that the dysregulation of cellular bioenergetics is associated with a wide array of common human diseases, including cancer, neurological diseases and diabetes. Respiration provides a vital source of cellular energy for most eukaryotic cells, particularly high energy dem...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20160083
更新日期：2016-06-30 00:00:00
abstract：:The secretory process is a coordinated cellular response, initiated by occupation of surface receptors and comprising an ordered sequence of biochemical steps subject to multiple controls. Conceptually we can divide the sequence into two main sections comprising early, receptor-mediated events leading to generation of...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1007/BF01362501
更新日期：1987-05-01 00:00:00
abstract：:Peptide sequencing of the complement system regulatory protein, factor H, permitted the synthesis of a mixed sequence oligonucleotide probe. Human liver cDNA libraries were screened and factor H-specific clones selected. No full-length clone was obtained, but the largest available clone, R2a, was found to encode the C...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01124790
更新日期：1987-03-01 00:00:00
abstract：:A consensus about the prognostic role of NIMA-related kinase 2 (NEK2) expression in various solid tumors has not been made yet. Thus, this meta-analysis aimed to systematically assess the prognostic role of NEK2 expression in patients with solid tumors. The eligible studies were identified through searching PubMed, We...
journal_title：Bioscience reports
pub_type： 杂志文章,meta分析
doi：10.1042/BSR20180618
更新日期：2019-01-18 00:00:00
abstract：:This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana [Pa-2 (P. americana lectin-2)] can also be intrinsically unordered, based on the results obtained by CD, tryptophan fluorescence, ANS (8-anilinonaphthalene-1-sulfonic acid) binding, acrylamide quenching, DLS (dynamic li...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20090035
更新日期：2009-11-23 00:00:00
abstract：:Previous studies demonstrated that the adaptive response to stressors and inflammatory signals involves the activation of the autonomous [corrected] nervous system. Catecholamines have been shown to modulate the activity of various immune effector cells directly via membrane adrenergic receptors. Here, we investigated...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1014768909442
更新日期：2001-10-01 00:00:00
