Diagnosis of enterovirus 70 infection by demonstration of IgM antibodies.

Abstract:

:Since outbreaks of severe acute hemorrhagic conjunctivitis occur worldwide [Hierholzer and Hatch, 1985] and the majority of the epidemics are caused by enterovirus 70 (EV-70), we developed an EV-70 IgM ELISA to simplify the diagnosis of these outbreaks. The test is based on the capture antibody technique and the use of monoclonal antibodies to EV-70. We detected EV-70 IgM antibodies in 55% of 76 convalescent-phase sera from an outbreak of acute hemorrhagic conjunctivitis in a Brazilian community. Among the 71 acute- and convalescent-phase serum pairs from this outbreak, 49 (69%) demonstrated a 4-fold or greater rise in neutralizing antibody-titer. The titer of IgM antibody began to drop by the fifth week after onset of illness. EV-70 IgM antibodies were not detected in 53 serum pairs with a 4-fold or greater rise in antibodies to other picornaviruses. The EV-70 ELISA proved to simple and relatively rapid to perform, appeared to be specific, and should be sensitive enough to diagnose outbreaks of EV-70 when multiple serum specimens can be tested.

journal_name

J Med Virol

authors

Wulff H,Anderson LJ,Pallansch MA,de Souza Carvalho RP

doi

10.1002/jmv.1890210404

subject

Has Abstract

pub_date

1987-04-01 00:00:00

pages

321-7

issue

4

eissn

0146-6615

issn

1096-9071

journal_volume

21

pub_type

杂志文章
  • Unconventional pathogens causing spongiform encephalopathies absent in blood products.

    abstract::To determine whether unconventional pathogens causing subacute spongiform encephalopathy may be present in blood products, a newly developed hepatitis B vaccine and a widely used blood product were injected into mice and rats. As only a few aged mice in the test and the control groups showed spongiform encephalopathic...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890160103

    authors: Tateishi J,Tsuji S

    更新日期:1985-05-01 00:00:00

  • Respiratory infections associated with enterovirus D68 from 2011 to 2015 in Beijing, China.

    abstract::Enterovirus D68 (EV-D68) is an emergent viral pathogen associated with mild to severe respiratory infections. In this study, we describe respiratory infections associated with EV-D68 in Beijing over a 4 year period. Total nucleic acid was extracted from 7,945 clinical specimens collected between January 5, 2011 and Ju...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24505

    authors: Zhang T,Li A,Chen M,Wu J,Huang F

    更新日期:2016-09-01 00:00:00

  • Prevalence of sapovirus infection among infants and children with acute gastroenteritis in Dhaka City, Bangladesh during 2004-2005.

    abstract::Sapovirus, a member of the family Caliciviridae is one of the major causative agents of viral gastroenteritis affecting all age group. Sapovirus was detected in 25 of 917 stool specimens from infants and children with acute gastroenteritis in a Children Hospital in Dhaka City, Bangladesh during 2004-2005. All fecal sp...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20859

    authors: Dey SK,Phan TG,Nguyen TA,Nishio O,Salim AF,Yagyu F,Okitsu S,Ushijima H

    更新日期:2007-05-01 00:00:00

  • HCV and HIV co-infection in pregnant women attending St. Camille Medical Centre in Ouagadougou (Burkina Faso).

    abstract::Five hundred and forty-seven pregnant women with less than 32 weeks of amenorrhoea, attending an antenatal clinic of St. Camille Medical Centre (SCMC) of Ouagadougou were enrolled for a hepatitis C virus (HCV) and HIV co-infection study. Fifty-eight (10.6%) were HIV positive and 18 (3.3%) were anti-HCV positive. Only ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20258

    authors: Simpore J,Ilboudo D,Samandoulougou A,Guardo P,Castronovo P,Musumeci S

    更新日期:2005-02-01 00:00:00

  • Significant impact of non-B HIV-1 variants genetic diversity in Gabon on plasma HIV-1 RNA quantitation.

    abstract::Evaluations of HIV-1 RNA viral load assays are lacking in Central Africa. The main objective of our study was to assess the reliability of HIV-1 RNA results obtained with three different assays for samples collected in Gabon. A total of 137 plasma specimens were assessed for HIV-1 RNA using the Abbott RealTime HIV-1® ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.23770

    authors: Mouinga-Ondémé A,Mabika-Mabika A,Alalade P,Mongo AD,Sica J,Liégeois F,Rouet F

    更新日期:2014-01-01 00:00:00

  • Polymerase chain reaction for prenatal diagnosis of congenital human cytomegalovirus infection.

    abstract::The reliability of the polymerase chain reaction (PCR) for prenatal diagnosis of human cytomegalovirus (HCMV) infection was determined by retrospective testing of 35 amniotic fluids identified previously as positive or negative for HCMV by virus isolation. Amniocentesis was performed in 26 pregnant women with primary ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890470428

    authors: Revello MG,Baldanti F,Furione M,Sarasini A,Percivalle E,Zavattoni M,Gerna G

    更新日期:1995-12-01 00:00:00

  • Molecular epidemiology of South African strains of hepatitis A virus: 1982-1996.

    abstract::Isolates of hepatitis A virus (HAV) are of a single serotype, with human isolates being categorised within four genotypes. In addition, there are three genotypes exclusively associated with Old World monkeys. In some geographical regions, related isolates cluster suggesting endemic spread of the virus, while in other ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9071(199704)51:4<273::aid-jmv3>

    authors: Taylor MB

    更新日期:1997-04-01 00:00:00

  • Clustering patterns of type-type combination in multiple genotypes infections of human papillomavirus in cervical adenocarcinoma.

    abstract::Coinfections with multiple types of human papillomavirus (HPV) occur in cervical adenocarcinoma (ADC). However, it remains unclear the clustering patterns of multiple types in HPV coinfections and relevant factors in ADC. A total of 718 paraffin-embedded ADC specimens were collected in China and tested for HPV genotyp...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25553

    authors: Dong L,Li T,Li L,Wang MZ,Wu Z,Cui J,Liu B,Zhang X,Qiao Y,Chen W

    更新日期:2019-11-01 00:00:00

  • Rotavirus prevalence and relationships with climatological factors in Gabon, Africa.

    abstract::A one-year study of rotavirus prevalence was carried out using electron microscopy in Gabon, equatorial Africa. One hundred fifty-six nonhospitalized diarrheic children up to 10 years of age and 115 age-matched controls were investigated together with diarrheic adults and children over 10 years of age. Rotaviruses wer...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890160210

    authors: Sitbon M,Lecerf A,Garin Y,Ivanoff B

    更新日期:1985-06-01 00:00:00

  • High seroprevalence of human herpesvirus 8 and herpes simplex virus 2 infections in men who have sex with men in Shanghai, China.

    abstract::Men who have sex with men (MSM) are an important risk group for human immunodeficiency virus (HIV) and at high-risk of herpesvirus infection. However, limited information on epidemiologic patterns of HHV8 and HSV2 among MSM is available in mainland China. A cross-sectional study of 486 participants was performed in Sh...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.24718

    authors: Liu Z,Fang Q,Zuo J,Wang J,Chen Y,Minhas V,Wood C,He N,Zhang T

    更新日期:2017-05-01 00:00:00

  • Rhinovirus frequently detected in elderly adults attending an emergency department.

    abstract::The general aim was to investigate the burden of respiratory virus illness in a hospital emergency department, during two different epidemic seasons. Consecutive patients attending an emergency department during two study periods (February/March 2009 and 2010) were enrolled using broad inclusion criteria (fever/preced...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22205

    authors: Pierangeli A,Scagnolari C,Selvaggi C,Verzaro S,Spina MT,Bresciani E,Antonelli G,Bertazzoni G

    更新日期:2011-11-01 00:00:00

  • Dynamic changes of HBV quasispecies and deletion patterns in a chronic hepatitis B patient.

    abstract::Hepatitis B virus (HBV) infection is a dynamic process during which molecular variants are selected continuously to adapt to changes. In addition to drug resistant mutations, sequential antiviral therapy may also lead to the selection of deleted mutants. To investigate this process, the following samples were collecte...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21595

    authors: Ji F,Zhou L,Ma S,Li F,Ding H,Zeng C

    更新日期:2009-09-01 00:00:00

  • Hepatitis B virus clearance from serum and liver after acute hepatitis delta virus superinfection in chronic HBsAg carriers.

    abstract::Clinical, virological, and histological findings in four HBsAg chronic carriers who cleared HBV markers from both serum and liver following HDV superinfection are described. The patients were long-term HBsAg carriers and all were HBV-DNA/HBeAg positive. Liver biopsy, obtained from three of the patients between 5 and 1...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890310408

    authors: Pastore G,Monno L,Santantonio T,Angarano G,Milella M,Giannelli A,Fiore JR

    更新日期:1990-08-01 00:00:00

  • Hepatitis B virus-DNA in the serum of patients followed-up longitudinally with acute and chronic hepatitis B.

    abstract::Sera from 79 patients with acute self-limiting hepatitis, 17 patients with acute hepatitis B evolving into chronic HBsAg carriership, and 43 chronic HBsAg carriers without a history of acute hepatitis were analyzed for presence of hepatitis B virus (HBV)-DNA by a molecular hybridization technique. In acute self-limiti...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.1890170406

    authors: Moestrup T,Hansson BG,Widell A,Blomberg J,Nordenfelt E

    更新日期:1985-12-01 00:00:00

  • Quantification of infectious duck hepatitis B virus by radioimmunofocus assay.

    abstract::A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with liquid overlay mediu...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Anderson DA,Grgacic EV,Luscombe CA,Gu X,Dixon R

    更新日期:1997-08-01 00:00:00

  • Profile of pre-S deletions in the natural history of chronic hepatitis B infection.

    abstract::It have been suggested that hepatitis B virus (HBV) pre-S deletions may play a role in hepatocarcinogenesis. The aim of the study was to determine the prevalence of pre-S deletions in chronic hepatitis B patients in Hong Kong, the factors associated with the deletions and its relationship with hepatitis B e antigen (H...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21901

    authors: Yeung P,Wong DK,Lai CL,Fung J,Seto WK,Yuen MF

    更新日期:2010-11-01 00:00:00

  • Viral genotypes and response to interferon in patients with acute prolonged hepatitis B virus infection of adulthood in Japan.

    abstract::Acute hepatitis B virus (HBV) infection was diagnosed in 57 adults admitted to Toranomon Hospital in Tokyo, Japan. Genotypes of HBV were determined by a serological method and compared to those in 1,077 patients with chronic hepatitis B. The distribution of genotypes were: genotype A (acute, 22.8% vs. chronic, 1.9%; P...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.10239

    authors: Kobayashi M,Arase Y,Ikeda K,Tsubota A,Suzuki Y,Saitoh S,Kobayashi M,Suzuki F,Akuta N,Someya T,Matsuda M,Sato J,Takagi K,Miyakawa Y,Kumada H

    更新日期:2002-12-01 00:00:00

  • Differential effect of amantadine hydrochloride on the systemic and local immune response to influenza A.

    abstract::Anti-influenza serum and nasal antibody titers were followed during a double-blind, placebo-controlled, randomized study assessing the prophylactic efficacy of 50, 100, and 200 mg/day of amantadine hydrochloride against experimental challenge with influenza A/Beth/1/85. The geometric mean titers (GMT) of serum hemaggl...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/jmv.1890270213

    authors: Reuman PD,Bernstein DI,Keely SP,Young EC,Sherwood JR,Schiff GM

    更新日期:1989-02-01 00:00:00

  • Reconnaissance of the candidate genes involved in the pathogenesis of human immunodeficiency virus and targeted by antiretroviral therapy.

    abstract::The expression levels of many genes change after treatment of human immunodeficiency virus (HIV)-infected subjects by antiretroviral drugs. High-throughput analysis of tremendous datasets led to the discovery of genes that are implicated in the treatment pathways. In this study, we performed a gene-enrichment analysis...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.25549

    authors: Mozhgani SH,Zarei Ghobadi M,Behnam Rad M,Farzanehpour M,Behzadian F

    更新日期:2019-12-01 00:00:00

  • Localization of CD8+ cells specific for hepatitis B virus surface protein in the liver of immunized mice.

    abstract::DNA plasmids are potent inducers of long-lasting antigen-specific CTL responses. Little is known about the distribution of antigen-specific CD8+ T cells in the lymphoid tissue and the non-lymphoid tissue after DNA immunization. HBsAg-specific CD8+ T cells in peripheral blood mononuclear cells, spleen, lymph nodes, and...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.21039

    authors: Qu D,Lanier G,Yuan ZH,Wen YM,Howard CR,Ahmed R

    更新日期:2008-02-01 00:00:00

  • Human T-cell lymphotropic virus antibody prevalence in HIV-1-infected individuals attending a sexual health clinic in South-East London.

    abstract::Human immunodeficiency virus (HIV) and human T-cell lymphotropic virus (HTLV) are both retroviruses with similar routes of transmission. A number of reports suggest variable clinical outcomes in HIV and HTLV co-infected individuals. There is no published information regarding the prevalence of HIV and HTLV co-infectio...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20336

    authors: Cooke FJ,Geretti AM,Zuckerman M

    更新日期:2005-06-01 00:00:00

  • Emergence of G3 and G9 rotavirus and increased incidence of mixed infections in the southern region of Ireland 2001-2004.

    abstract::Two hundred and thirty fecal specimens were collected from children (up to 5 years of age) admitted with suspected rotaviral gastroenteritis at four Irish hospitals (Cork University Hospital, Mercy Hospital, Cork, Waterford Regional Hospital, and Kerry General Hospital) in the southern region of Ireland, between 2001 ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20494

    authors: Reidy N,O'Halloran F,Fanning S,Cryan B,O'Shea H

    更新日期:2005-12-01 00:00:00

  • Screening for vaginal shedding of cytomegalovirus in healthy pregnant women using real-time PCR: correlation of CMV in the vagina and adverse outcome of pregnancy.

    abstract::The impact of cytomegalovirus (CMV) infection of the genital tract during pregnancy on adverse pregnancy outcomes is not understood fully. A real-time PCR assay was used to determine vaginal shedding of CMV in 993 healthy pregnant Japanese women and the results were compared with the outcome of pregnancy. CMV DNA was ...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20619

    authors: Tanaka K,Yamada H,Minami M,Kataoka S,Numazaki K,Minakami H,Tsutsumi H

    更新日期:2006-06-01 00:00:00

  • Antibody persistence and immune memory in adults, 15 years after a three-dose schedule of a combined hepatitis A and B vaccine.

    abstract::A combined hepatitis A and B vaccine is available since 1996. Two separate open-label primary studies evaluated the immunogenicity and safety of this hepatitis A and B vaccine (720 EI.U of HAV and 20 µg of HBsAg) in 306 healthy subjects aged 17-43 years who received three doses of the vaccine following a 0, 1, and 6 m...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.22264

    authors: Van Damme P,Leroux-Roels G,Crasta P,Messier M,Jacquet JM,Van Herck K

    更新日期:2012-01-01 00:00:00

  • Prevalence of unusual human rotavirus strains in Ghanaian children.

    abstract::Sixty-seven rotavirus-positive fecal samples, collected between January and April 1999, from children with diarrhea in the Upper East Region of Ghana were examined for rotavirus VP7 and VP4 types. Sufficient viral RNA could be obtained from 46 (68.7%) of the samples and all the isolates had short electrophoretic patte...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:

    authors: Armah GE,Pager CT,Asmah RH,Anto FR,Oduro AR,Binka F,Steele D

    更新日期:2001-01-01 00:00:00

  • Changes in the hemagglutinins and neuraminidases of human influenza B viruses isolated in Italy during the 2001-02, 2002-03, and 2003-04 seasons.

    abstract::Throughout most of the last decade, B/Yamagata/16/88-lineage influenza viruses were predominant among the B isolates circulating worldwide, whereas B/Victoria/2/87-lineage viruses were isolated infrequently and restricted geographically to eastern Asia. During the 2001-02 influenza season, B/Victoria/2/87-lineage viru...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20225

    authors: Puzelli S,Frezza F,Fabiani C,Ansaldi F,Campitelli L,Lin YP,Gregory V,Bennett M,D'Agaro P,Campello C,Crovari P,Hay A,Donatelli I

    更新日期:2004-12-01 00:00:00

  • Switching to tenofovir disoproxil fumarate vs continuing treatment in patients with chronic hepatitis B who maintain long-term virological response to entecavir therapy: A randomized trial.

    abstract::No controlled trial in patients with chronic hepatitis B virus (HBV) infection on long-term entecavir (ETV) treatment, comparing switching to tenofovir disoproxil fumarate (TDF) with continuing the therapy, has been reported. Twenty-seven nucleos(t)ide-naïve patients with chronic HBV who underwent ETV therapy for ≥5 y...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/jmv.25442

    authors: Iida-Ueno A,Enomoto M,Kozuka R,Tamori A,Kawada N

    更新日期:2019-07-01 00:00:00

  • Scoring system for risk stratification of viral reactivation during prophylactic antiviral treatment in Korean patients with hepatitis B undergoing anticancer chemotherapy: A multicenter study.

    abstract::Prophylactic antiviral therapy is recommended for hepatitis B virus (HBV)-infected patients with malignancies who are undergoing systemic chemotherapy. In the current study, we aimed to develop a risk scoring system to guide the selection of prophylactic antiviral agents. In this retrospective analysis, we included co...

    journal_title:Journal of medical virology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/jmv.25241

    authors: Kim HY,Yoo JJ,Oh S,Yu SJ,Kim YJ,Yoon JH,Kim W,Jung YJ,Kim BH,Kim CM,Park JW,Lee JH

    更新日期:2018-10-01 00:00:00

  • Effects of drug resistance on viral load in patients failing antiretroviral therapy.

    abstract::Previous studies on patients who develop drug resistant HIV-1 variants have shown that continued use of failing regimens might provide clinical benefit. However, the effect of long-term exposure to drug resistant variants may lead to emergence of compensatory mutations that may jeopardize this effect. In this study, w...

    journal_title:Journal of medical virology

    pub_type: 杂志文章

    doi:10.1002/jmv.20582

    authors: Machouf N,Thomas R,Nguyen VK,Trottier B,Boulassel MR,Wainberg MA,Routy JP

    更新日期:2006-05-01 00:00:00

  • Effects of ribavirin on intrahepatic and extrahepatic expression of hepatitis C virus in interferon nonresponsive patients.

    abstract::Response to ribavirin therapy (1,000-1,200 mg/day for 6 months) was evaluated in nine patients with chronic hepatitis C virus (HCV) infections who had previously failed to respond to a 6-month course of alpha-interferon. All had chronic active hepatitis with elevated serum aminotransferase activities (mean +/- SD = 13...

    journal_title:Journal of medical virology

    pub_type: 临床试验,杂志文章

    doi:10.1002/jmv.1890450106

    authors: Koskinas J,Tibbs C,Saleh MG,Pereira LM,McFarlane IG,Williams R

    更新日期:1995-01-01 00:00:00