Abstract:
:Vitamin D is known to have immunomodulatory effects, is involved in osteo-cartilaginous metabolism, and may have a role in human intervertebral disc pathophysiology. Although a link between vitamin D receptor (VDR) gene variants and disc degeneration-related pathologies has been observed, its functional contribution to pathologic processes has not been assessed yet. The aim of this study was to investigate the response of disc cells to vitamin D in terms of the regulation of proliferation, metabolism, and inflammatory processes, with a particular focus on the FokI VDR genotype. However, although it was found that vitamin D had a pro-apoptotic effect regardless of genotype, an up-regulation of IL-1Ra and downregulation of IL-6 was found to be evident only in Ff cells. Regarding the metabolic effects, in Ff cells, vitamin D promoted an upregulation of the aggrecan in inflammatory conditions but did not have an effect on the expression of collagen-related markers. Moreover, cells bearing the Ff genotype were the most responsive to vitamin D in the upregulation of catabolic markers. In addition, in contrast to the FF genotype, vitamin D downregulated the vitamin D-dependent signaling pathway in inflamed Ff cells, counteracting the inflammation-mediated catabolic effects. In conclusion, Ff cells were found to be more responsive to the anti-inflammatory and catabolic effects of vitamin D, which is likely to be related to matrix remodeling.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
De Luca P,de Girolamo L,Perucca Orfei C,Viganò M,Cecchinato R,Brayda-Bruno M,Colombini Adoi
10.3390/ijms19072002subject
Has Abstractpub_date
2018-07-09 00:00:00issue
7issn
1422-0067pii
ijms19072002journal_volume
19pub_type
杂志文章abstract::Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signaling pathways. However, whether it can inhibit human pancreatic carcinoma growth and what are the underlying mechanisms is still not well elucidated....
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12084861
更新日期:2011-01-01 00:00:00
abstract::Molecular testing offers more objective information in the diagnosis and personalized decision making for thyroid nodules. In Korea, as the BRAF V600E mutation is detected in 70-80% of thyroid cancer specimens, its testing in fine-needle aspiration (FNA) cytology specimens alone has been used for the differential diag...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165629
更新日期:2020-08-06 00:00:00
abstract::Förster resonance energy transfer (FRET) is a powerful tool used for many problems in membrane biophysics, including characterization of the lateral distribution of lipid components and other species of interest. However, quantitative analysis of FRET data with a topological model requires adequate choices for the val...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131114545
更新日期:2012-11-08 00:00:00
abstract::Prediction of microRNA-mRNA interaction typically relies on bioinformatic methods, but these methods only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. A major obstacle to the miRNA research has been the lack of experimental procedures for the identi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150814753
更新日期:2014-08-22 00:00:00
abstract::Fractalkine/CX3C chemokine ligand 1 (CX3CL1) is a chemokine involved in the anticancer function of lymphocytes-mainly NK cells, T cells and dendritic cells. Its increased levels in tumors improve the prognosis for cancer patients, although it is also associated with a poorer prognosis in some types of cancers, such as...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21103723
更新日期:2020-05-25 00:00:00
abstract::AMP-activated protein kinase (AMPK) is an important metabolic regulator that mediates cellular adaptation to diverse stresses. One of the AMPK substrates, tuberous sclerosis complex 2 (TSC2), was suggested to mediate AMPK-induced silencing of mTOR complex 1 (mTORC1) signaling that is critical for cell growth. However,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16047015
更新日期:2015-03-27 00:00:00
abstract::Understanding the kinetics of peptide self-assembly is important because of the involvement of peptide amyloid fibrils in several neurodegenerative diseases. In this paper, we have studied the dissolution kinetics of self-assembled model peptide fibrils after a dilution quench. Due to the low concentrations involved, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207671
更新日期:2020-10-16 00:00:00
abstract:BACKGROUND:P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was sugg...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184373
更新日期:2019-09-06 00:00:00
abstract::This paper aims at analysing the synthesis of flavonoids, their import and export in plant cell compartments, as well as their involvement in the response to stress, with particular reference to grapevine (Vitis vinifera L.). A multidrug and toxic compound extrusion (MATE) as well as ABC transporters have been demonst...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140714950
更新日期:2013-07-17 00:00:00
abstract::Autocrine signaling is defined as the production and secretion of an extracellular mediator by a cell followed by the binding of that mediator to receptors on the same cell to initiate signaling. Autocrine stimulation often operates in autocrine loops, a type of interaction, in which a cell produces a mediator, for wh...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020977
更新日期:2021-01-19 00:00:00
abstract::Phosphatidylinositol 3-kinase (PI3K) is a key enzyme that phosphorylates phosphatidylinositol at 3'-hydroxyl position of the inositol head group initiating the generation of several phosphorylated phosphatidylinositols, collectively referred to as phosphoinositides. The function of PI3K in plant senescence and ethylen...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18071533
更新日期:2017-07-15 00:00:00
abstract::Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophage...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161226145
更新日期:2015-12-04 00:00:00
abstract::Previously, we showed that lipocalin2 (LCN2) serum levels increased after liver irradiation and during acute-phase conditions. Here, we evaluate LCN2 expression and serum levels after single-dose lung irradiation with 25 Gy, percutaneously administered to the lung of randomly-paired male Wistar rats. Due to the concav...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17050637
更新日期:2016-04-28 00:00:00
abstract::Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells. Extracellular polysaccharides play an important role in matrix setup and maintenance. A water-insoluble polysaccharide was isolated and p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051702
更新日期:2020-03-02 00:00:00
abstract::The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cispl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12063489
更新日期:2011-01-01 00:00:00
abstract::Cerium dioxide nanoparticles (C(e)O₂ ENPs) are on the priority list of nanomaterials requiring evaluation. We performed in vitro assays on mature mouse oocytes incubated with C(e)O₂ ENPs to study (1) physicochemical biotransformation of ENPs in culture medium; (2) ultrastructural interactions with follicular cells and...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141121613
更新日期:2013-10-31 00:00:00
abstract::In this study we used a laser ablation technique for preparation of silver nanoparticles. The fabrication process was carried out by ablation of a silver plate immersed in palm oil. A pulsed Nd:YAG laser at a wavelength of 1064 nm was used for ablation of the plate at different times. The palm coconut oil allowed form...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11114764
更新日期:2010-11-22 00:00:00
abstract::Vitamin D receptor (VDR) is present in multiple blood cells, and the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D) is essential for the proper functioning of the immune system. The role of retinoic acid receptor α (RARα) in hematopoiesis is very important, as the fusion of RARα gene with PML gene initiat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061323
更新日期:2017-06-21 00:00:00
abstract::The overexpressing ABCB1 transporter is one of the key factors leading to multidrug resistance (MDR). Thus, many ABCB1 inhibitors have been found to be able to overcome ABCB1-mediated MDR. However, some inhibitors also work as a substrate of ABCB1, which indicates that in order to achieve an effective reversal dosage,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041387
更新日期:2020-02-19 00:00:00
abstract::Calcific aortic valve disease (CAVD) is the result of maladaptive fibrocalcific processes leading to a progressive thickening and stiffening of aortic valve (AV) leaflets. CAVD is the most common cause of aortic stenosis (AS). At present, there is no effective pharmacotherapy in reducing CAVD progression; when CAVD be...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21238917
更新日期:2020-11-24 00:00:00
abstract::Carotid atherosclerotic plaque rupture can lead to cerebrovascular accident (CVA). By comparing RNA-Seq data from vascular smooth muscle cells (VSMC) extracted from carotid atheroma surgically excised from a group of asymptomatic and symptomatic subjects, we identified more than 700 genomic variants associated with sy...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249387
更新日期:2020-12-09 00:00:00
abstract::Atherosclerosis, as a chronic inflammatory disease, is the major underlying cause of death worldwide. However, the mechanisms that underlie the inflammatory process are not completely understood. Prolyl-isomerase-1 (Pin1), as a unique peptidyl-prolyl isomerase, plays an important role in inflammation and endothelial d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18030644
更新日期:2017-03-16 00:00:00
abstract::A thorough characterization of the early time sub-100 fs relaxation dynamics of biologically relevant chromophores is of crucial importance for a complete understanding of the mechanisms regulating the ultrafast dynamics of the relaxation processes in more complex multichromophoric light-harvesting systems. While chlo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082836
更新日期:2020-04-18 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is known to regulate mitochondrial biogenesis. Resveratrol is present in a variety of plants, including the skin of grapes, blueberries, raspberries, mulberries, and peanuts. It has been shown to offer protective effects against a number of ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20040998
更新日期:2019-02-25 00:00:00
abstract::Pompe disease is a glycogen storage disease caused by a deficiency in acid α-glucosidase (GAA), a hydrolase necessary for the degradation of lysosomal glycogen. This deficiency in GAA results in muscle and neuronal glycogen accumulation, which causes respiratory insufficiency. Pompe disease mouse models provide a mean...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21062256
更新日期:2020-03-24 00:00:00
abstract::The treatment of metastatic breast cancer remained a challenge despite the recent breakthrough in the immunotherapy regimens. Here, we addressed the multidimensional immunophenotyping of 4T1 metastatic breast cancer by the state-of-the-art single cell mass cytometry (CyTOF). We determined the dose and time dependent c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010170
更新日期:2019-12-25 00:00:00
abstract::Jatropha curcas is a promising renewable feedstock for biodiesel and bio-jet fuel production. To study gene expression in Jatropha in different tissues throughout development and under stress conditions, we examined a total of 11 typical candidate reference genes using real-time quantitative polymerase chain reaction ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141224338
更新日期:2013-12-13 00:00:00
abstract::The design, construction and initial tests on a combined laser light scattering and synchrotron X-ray scattering instrument can cover studies of length scales from atomic sizes in Angstroms to microns and dynamics from microseconds to seconds are presented. In addition to static light scattering (SLS), dynamic light s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160510016
更新日期:2015-05-04 00:00:00
abstract::In the present study, IL-1β cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length IL-1β mRNA was obtained using Rapid Amplification of cDNA Ends (RACE), which contains 78 bp 3'-UTR, a 455 bp 5'-UTR, and an open reading frame (ORF) of 702 bp coding for 233 amino acid residues. The m...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101670
更新日期:2016-10-01 00:00:00
abstract::Reptiles are a rare model object for space research. However, some reptile species demonstrate effective adaptation to spaceflight conditions. The main scope of this review is a comparative analysis of reptile experimental exposure in weightlessness, demonstrating the advantages and shortcomings of this model. The des...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20123019
更新日期:2019-06-20 00:00:00