Abstract:
:Prediction of microRNA-mRNA interaction typically relies on bioinformatic methods, but these methods only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. A major obstacle to the miRNA research has been the lack of experimental procedures for the identification of miRNA-mRNA interactions. Recently, a few studies have attempted to explore experimental methods to isolate and identify miRNA targets or miRNAs targeting a single gene. Here, we developed an more convenient experimental approach for the isolation and identification of miRNAs targeting a single gene by applying short biotinylated DNA anti-sense oligonucleotides mix to enhanced green fluorescent protein (EGFP) mRNA which was fused to target gene mRNA. This method does not require a design of different anti-sense oligonucleotides to any mRNA. This is a simple and an efficient method to potentially identify miRNAs targeting specific gene mRNA combined with chip screen.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wei K,Yan F,Xiao H,Yang X,Xie G,Xiao Y,Wang T,Xun Y,Huang Z,Han M,Zhang J,Xiang Sdoi
10.3390/ijms150814753subject
Has Abstractpub_date
2014-08-22 00:00:00pages
14753-65issue
8issn
1422-0067pii
ijms150814753journal_volume
15pub_type
杂志文章abstract::Gelam honey exerts anti-inflammatory and antioxidant activities and is thought to have potent effects in reducing infections and healing wounds. The aim of this study was to investigate the effects of intravenously-injected Gelam honey in protecting organs from lethal doses of lipopolysaccharide (LPS). Six groups of r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13056370
更新日期:2012-01-01 00:00:00
abstract::It is an important but also a challenging analytical problem to understand the chemical composition and structure of prebiotic organic matter that is present in extraterrestrial materials. Its formation, evolution and content in the building blocks ("seeds") for more complex molecules, such as proteins and DNA, are ke...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17040439
更新日期:2016-03-24 00:00:00
abstract::Amino acid transporters alanine-serine-cysteine transporter 2 (ASCT2) and L-Type Amino Acid Transporter 1 (LAT1) are coordinately enhanced in human cancers where among other roles, they are thought to drive mechanistic target-of-rapamycin (mTOR) growth signaling. To assess ASCT2 and LAT1 as therapeutic targets, nine u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072093
更新日期:2018-07-19 00:00:00
abstract::Diabetic nephropathy (DN) is the leading cause of end-stage renal disease in diabetes mellitus. Oxidative stress, insulin resistance and pro-inflammatory cytokines have been shown to play an important role in pathogeneses of renal damage on type 2 diabetes mellitus (DM). Inonotus obliquus (IO) is a white rot fungus th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17091535
更新日期:2016-09-13 00:00:00
abstract::Growing evidence shows a close association of transposable elements (TE) with non-coding RNAs (ncRNA), and a significant number of small ncRNAs originate from TEs. Further, ncRNAs linked with TE sequences participate in a wide-range of regulatory functions. Alu elements in particular are critical players in gene regul...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140713307
更新日期:2013-06-26 00:00:00
abstract::The spleen plays an important role in coordinating both adaptive and innate immune responses. Here, the transcriptional response to T. gondii infection in the murine spleen was characterized concerning inflammasome sensors (two different models: seven days after oral or four weeks after intraperitoneal infection). Add...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061245
更新日期:2017-06-10 00:00:00
abstract::Chlorotoxin (CTX) is a 36⁻amino acid peptide with eight Cys residues that forms four disulfide bonds. It has high affinity for the glioma-specific chloride channel and matrix metalloprotease-2. Structural and binding properties of CTX analogs with various Cys residue substitutions with l-α-aminobutyric acid (Abu) have...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061261
更新日期:2019-03-13 00:00:00
abstract::Staphylococcus aureus is able to rapidly develop mechanisms of resistance to various drugs and to form strong biofilms, which makes it necessary to develop new antibacterial drugs. The essential oil of Melaleuca alternifolia is used as an antibacterial, a property believed to be mainly due to the presence of terpinen-...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124531
更新日期:2020-06-25 00:00:00
abstract::Apolipoprotein E (apoE) is mainly secreted by hepatocytes and incorporated into most plasma lipoproteins. Macrophages, which accumulate cholesterol and are critical for the development of the atherosclerotic plaque, are also an important, albeit smaller, apoE source. Distal regulatory elements control cell-specific ac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061471
更新日期:2019-03-23 00:00:00
abstract::Chitosan is a versatile compound with multiple biotechnological applications. This polymer inhibits clinically important human fungal pathogens under the same carbon and nitrogen status as in blood. Chitosan permeabilises their high-fluidity plasma membrane and increases production of intracellular oxygen species (ROS...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20020332
更新日期:2019-01-15 00:00:00
abstract::Ion channels included in the family of Connexins (Cx) help to control cell proliferation and differentiation of neuronal progenitors. Here we explored the role of Connexin 50 (Cx50) in cell fate modulation of adult spinal cord derived neural precursors located in the ependymal canal (epSPC). epSPC from non-injured ani...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161125981
更新日期:2015-11-06 00:00:00
abstract::Heart failure (HF) is a rapidly growing global public health problem. Since HF results in high mortality and re-hospitalization, new effective treatments are desired. Although it remains controversial, omega 3 polyunsaturated fatty acids (n-3 PUFAs), such as the eicosapentaenoic acid and docosahexaenoic acid, have bee...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20164025
更新日期:2019-08-18 00:00:00
abstract::WRKY transcription factors constitute one of the largest transcription factor families in plants, and play crucial roles in plant growth and development, defense regulation and stress responses. However, knowledge about this family in maize is limited. In the present study, we identified a drought-induced WRKY gene, Z...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19103046
更新日期:2018-10-06 00:00:00
abstract::In recent decades, serious contamination of soils by heavy metals has been reported. It is therefore a matter of urgency to develop a new and efficient technology for removing contaminants from soil. Another aspect to this problem is that environmental pollution decreases the biological quality of soil, which is why p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12117760
更新日期:2011-01-01 00:00:00
abstract::Glioblastoma is the most common malignant primary brain tumor, and it is one of the causes of cancer fatality in both adult and pediatric populations. Patients with glioblastoma require chemotherapy after surgical resection and radiotherapy. Therefore, chemotherapy constitutes a viable approach for the eradication of ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18091945
更新日期:2017-09-11 00:00:00
abstract::It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of the mechanisms underl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms130911895
更新日期:2012-01-01 00:00:00
abstract::Mathematical optimization framework allows the identification of certain nodes within a signaling network. In this work, we analyzed the complex extracellular-signal-regulated kinase 1 and 2 (ERK1/2) cascade in cardiomyocytes using the framework to find efficient adjustment screws for this cascade that is important fo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20092179
更新日期:2019-05-02 00:00:00
abstract::Diabetic cardiomyopathy (DCM) has emerged as a relevant cause of heart failure among the diabetic population. Defined as a cardiac dysfunction that develops in diabetic patients independently of other major cardiovascular risks factors, such as high blood pressure and coronary artery disease, the underlying cause of D...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20112833
更新日期:2019-06-11 00:00:00
abstract::The interactions of plants with environment and insects are bi-directional and dynamic. Consequently, a myriad of mechanisms has evolved to engage organisms in different types of interactions. These interactions can be mediated by allelochemicals known as volatile organic compounds (VOCs) which include volatile terpen...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197382
更新日期:2020-10-06 00:00:00
abstract::Genetically modified vaccinia viruses (VACVs) have been shown to possess profound oncolytic capabilities. However, tumor cell resistance to VACVs may endanger broad clinical success. Using cell mass assays, viral replication studies, and fluorescence microscopy, we investigated primary resistance phenomena of cell lin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207618
更新日期:2020-10-15 00:00:00
abstract::This review summarizes available data on argan fruit botany, geographical distribution, traditional uses, environmental interest, socioeconomic role, phytochemistry, as well as health beneficial effects and examination of future prospects. In particular, ethnomedical uses of argan fruits are carried out throughout Mor...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18112277
更新日期:2017-10-30 00:00:00
abstract::Osteoporosis is characterized by a reduction of the bone mineral density (BMD) and microarchitectural deterioration of the bone, which lead to bone fragility and susceptibility to fracture. Astaxanthin (AST) has a variety of biological activities, such as a protective effect against asthma or neuroinflammation, antiox...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030912
更新日期:2018-03-19 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is characterized by the presence of steatosis, inflammation and hepatocyte injury and constitutes hepatic manifestation of the metabolic syndrome. The pathogenesis of NASH is complex and implicates cross-talk between different metabolically active sites, such as liver and adipose t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms141019867
更新日期:2013-10-01 00:00:00
abstract::Coffee is a popular drink consumed all over the world. Besides its long-recognized stimulant effect, it has important nutritional and health effects. However, the type of bean processing modifies the composition of brewed coffee and possibly its bioactivity. In this study, extracts obtained from green and roasted bean...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113407
更新日期:2018-10-31 00:00:00
abstract::Grass carp (Ctenopharyngodon idellus) is an important worldwide commercial freshwater culture species. However, grass carp reovirus (GCRV) causes serious hemorrhagic disease in fingerlings and yearlings of fishes. To understand the molecular pathogenesis of host cells during GCRV infection, intensive proteomic quantif...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112419
更新日期:2017-11-14 00:00:00
abstract::Exceptionally long-lived individuals (ELLI) who are the focus of many healthy longevity studies around the globe are now being studied in Israel. The Israeli Multi-Ethnic Centenarian Study (IMECS) cohort is utilized here for assessment of various DNA methylation clocks. Thorough phenotypic characterization and whole b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020615
更新日期:2020-01-17 00:00:00
abstract::Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20133135
更新日期:2019-06-27 00:00:00
abstract::Excess energy is stored primarily as triglycerides, which are mobilized when demand for energy arises. Dysfunction of energy balance by excess food intake leads to metabolic diseases, such as obesity and diabetes. Free fatty acids (FFAs) provided by dietary fat are not only important nutrients, but also contribute key...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17040450
更新日期:2016-03-25 00:00:00
abstract::The introduction of Clearfield technology allows the use of imidazolinone (IMI) herbicides to control weedy rice. Imidazolinone herbicides stop the acetolactate synthase (ALS) enzyme from synthesizing branched-chain amino acids, resulting in the death of the plant. Since the launch of Clearfield technology in Malaysia...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22030982
更新日期:2021-01-20 00:00:00
abstract::Human cytomegalovirus (HCMV) is a ubiquitous betaherpesvirus that frequently causes morbidity and mortality in individuals with insufficient immunity, such as transplant recipients, AIDS patients, and congenitally infected newborns. Several antiviral drugs are approved to treat HCMV infections. However, resistant HCMV...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20071636
更新日期:2019-04-02 00:00:00