Identification and order of sequential mutations in beta-actin genes isolated from increasingly tumorigenic human fibroblast strains.

abstract：:Clinical and epidemiological synergy exists between the globally important sexually transmitted infections, gonorrhea and HIV. Neisseria gonorrhoeae, which causes gonorrhea, is particularly adept at driving HIV-1 expression, but the molecular determinants of this relationship remain undefined. N. gonorrhoeae liberates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malott RJ,Keller BO,Gaudet RG,McCaw SE,Lai CC,Dobson-Belaire WN,Hobbs JL,St Michael F,Cox AD,Moraes TF,Gray-Owen SD

更新日期：2013-06-18 00:00:00

abstract：:Schaftoside and isoschaftoside are bioactive natural products widely distributed in higher plants including cereal crops and medicinal herbs. Their biosynthesis may be related with plant defense. However, little is known on the glycosylation biosynthetic pathway of these flavonoid di-C-glycosides with different sugar ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang ZL,Gao HM,Wang S,Zhang M,Chen K,Zhang YQ,Wang HD,Han BY,Xu LL,Song TQ,Yun CH,Qiao X,Ye M

更新日期：2020-12-01 00:00:00

abstract：:Natural exposure to prion disease is likely to occur throughout successive challenges, yet most experiments focus on single large doses of infectious material. We analyze the results from an experiment in which rodents were exposed to multiple doses of feed contaminated with the scrapie agent. We formally define hypot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gravenor MB,Stallard N,Curnow R,McLean AR

更新日期：2003-09-16 00:00:00

abstract：:Animal epithelial tissue becomes reproducibly colonized by specific environmental bacteria. The bacteria (microbiota) perform critical functions for the host's tissue development, immune system development, and nutrition; yet the processes by which bacterial diversity in the environment is selected to assemble the cor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brooks JF 2nd,Gyllborg MC,Cronin DC,Quillin SJ,Mallama CA,Foxall R,Whistler C,Goodman AL,Mandel MJ

更新日期：2014-12-02 00:00:00

abstract：:Intermediate filaments-cytoplasmic filaments with a diameter of 8-9 nm-have been described in a large variety of cell types. In this study, the subunit protein of the neurofilament and the presumptive subunit of the astroglial filament are compared by immunological and biochemical methods and are found to be very simi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yen SH,Dahl D,Schachner M,Shelanski ML

更新日期：1976-02-01 00:00:00

abstract：:One of the main differences between bacteria and archaea concerns their membrane composition. Whereas bacterial membranes are made up of glycerol-3-phosphate ester lipids, archaeal membranes are composed of glycerol-1-phosphate ether lipids. Here, we report the construction of a stable hybrid heterochiral membrane thr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caforio A,Siliakus MF,Exterkate M,Jain S,Jumde VR,Andringa RLH,Kengen SWM,Minnaard AJ,Driessen AJM,van der Oost J

更新日期：2018-04-03 00:00:00

abstract：:The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted. Dynamin is a GTPase shown to play an important role in the control clathrin mediated endocytosis of EGFR and other cell surface receptors. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sousa LP,Lax I,Shen H,Ferguson SM,De Camilli P,Schlessinger J

更新日期：2012-03-20 00:00:00

abstract：:Insertion of trocars, needles, and catheters into unintended tissues or tissue compartments results in hundreds of thousands of complications annually. Current methods for blood vessel cannulation or epidural, chest tube, and initial trocar placement often involve the blind pass of a needle through several layers of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bassett EK,Slocum AH,Masiakos PT,Pryor HI 2nd,Farokhzad OC,Karp JM

更新日期：2009-04-07 00:00:00

abstract：:Unintended block of HERG K+ channels is a side effect of many common medications and is the most common cause of acquired long QT syndrome associated with increased risk of life-threatening arrhythmias. The molecular mechanism of high-affinity HERG block by structurally diverse compounds has been attributed to pi-stac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen J,Seebohm G,Sanguinetti MC

更新日期：2002-09-17 00:00:00

abstract：:Three classes of heterogeneous nuclear RNA (HnRNA) alpha, beta, and gamma, with different chemical and physical properties, can be identified in the early sea urchin embryo by hybridization with poly(U). The relative amounts of these classes vary as a function of embryonic development. It is demonstrated here that the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dubroff LM

更新日期：1977-06-01 00:00:00

abstract：:Understanding the ecological impacts of climate change is a crucial challenge of the twenty-first century. There is a clear lack of general rules regarding the impacts of global warming on biota. Here, we present a metaanalysis of the effect of climate change on body size of ectothermic aquatic organisms (bacteria, ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daufresne M,Lengfellner K,Sommer U

更新日期：2009-08-04 00:00:00

abstract：:The results of electron-nuclear double resonance and electron paramagnetic resonance (EPR) studies on the hydrogen peroxide compound of yeast cytochrome c peroxidase are inconsistent with previous proposals for the source of the EPR signal in this compound, in particular with its identification with an aromatic amino ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman BM,Roberts JE,Brown TG,Kang CH,Margoliash E

更新日期：1979-12-01 00:00:00

abstract：:Many and diverse modifications of the myosin subfragment 1 (S-1) increase (modulate) its ATPase activity, including interaction of this particle with actin; a recent addition to these modifications is the extensive lysine modification of S-1 that seems prerequisite to crystallizing it for structure analysis. In this s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bivin DB,Ue K,Khoroshev M,Morales M

更新日期：1994-08-30 00:00:00

abstract：:Retroviral entry into cells depends on envelope glycoproteins, whereby receptor binding to the surface-exposed subunit triggers membrane fusion by the transmembrane protein (TM) subunit. We determined the crystal structure at 2.5-A resolution of the ectodomain of gp21, the TM from human T cell leukemia virus type 1. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobe B,Center RJ,Kemp BE,Poumbourios P

更新日期：1999-04-13 00:00:00

abstract：:A series of small peptides corresponding to the amino termini of the fibrin alpha- and beta-chains has been synthesized. The peptides glycyl-L-prolyl-L-arginyl-L-proline and glycyl-L-prolyl-L-arginylsarcosine are potent inhibitors of fibrin polymerization. Moreover, these peptides have a natural stability stemming fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laudano AP,Doolittle RF

更新日期：1978-07-01 00:00:00

abstract：:The microvillus core-filament bundle from intestinal epithelial cells is a highly ordered structure containing actin and four major associated proteins. Two of these, villin and calmodulin, bind calcium ions (Kd approximately 10(-6) M) in the physiologically important range. Because ethylene glycol bis(beta-aminoethyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glenney JR Jr,Bretscher A,Weber K

更新日期：1980-11-01 00:00:00

abstract：:Light microscopy autoradiography has been used to visualize neuroanatomical patterns of brain opiate receptor upregulation in response to chronic naltrexone administration. Slide-mounted brain sections of frozen rat brain were labeled in vitro with dihydro[3H]morphine, a relatively selective mu opioid ligand. The grea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tempel A,Gardner EL,Zukin RS

更新日期：1984-06-01 00:00:00

abstract：:Metabolomic markers associated with incident central adiposity gain were investigated in young adults. In a 9-mo prospective study of university freshmen (n = 264). Blood samples and anthropometry measurements were collected in the first 3 d on campus and at the end of the year. Plasma from individuals was pooled by p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hootman KC,Trezzi JP,Kraemer L,Burwell LS,Dong X,Guertin KA,Jaeger C,Stover PJ,Hiller K,Cassano PA

更新日期：2017-05-23 00:00:00

abstract：:In this paper we extend the well-known Vitali-Hahn-Saks and Nikodým theorems for measures to finitely additive vector-valued set functions. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brooks JK,Jewett RS

更新日期：1970-11-01 00:00:00

abstract：:1-Aminocyclopropane-1-carboxylate (ACC) synthase (EC 4.4.1.14), extracted from tomato pericarp tissue, was purified 6500-fold by conventional and high-performance liquid chromatography. Two-dimensional gel electrophoresis of this preparation indicated that ACC synthase activity was associated with a protein band at 50...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bleecker AB,Kenyon WH,Somerville SC,Kende H

更新日期：1986-10-01 00:00:00

abstract：:Particles composed of secondary organic material (SOM) are abundant in the lower troposphere. The viscosity of these particles is a fundamental property that is presently poorly quantified yet required for accurate modeling of their formation, growth, evaporation, and environmental impacts. Using two unique techniques...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renbaum-Wolff L,Grayson JW,Bateman AP,Kuwata M,Sellier M,Murray BJ,Shilling JE,Martin ST,Bertram AK

更新日期：2013-05-14 00:00:00

abstract：:Increased cardiovascular mortality occurs in diabetic patients with or without coronary artery disease and is attributed to the presence of diabetic cardiomyopathy. One potential mechanism is hyperglycemia that has been reported to activate protein kinase C (PKC), preferentially the beta isoform, which has been associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakasaki H,Koya D,Schoen FJ,Jirousek MR,Ways DK,Hoit BD,Walsh RA,King GL

更新日期：1997-08-19 00:00:00

abstract：:A codon-optimized mouse LINE-1 element, ORFeus, exhibits dramatically higher retrotransposition frequencies compared with its native long interspersed element 1 counterpart. To establish a retrotransposon-mediated mouse model with regulatable and potent mutagenic capabilities, we generated a tetracycline (tet)-regulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Donnell KA,An W,Schrum CT,Wheelan SJ,Boeke JD

更新日期：2013-07-16 00:00:00

abstract：:Memory stretches over a lifetime. In controlled laboratory settings, the hippocampus and other medial temporal lobe brain structures have been shown to represent space and time on the scale of meters and seconds. It remains unclear whether the hippocampus also represents space and time over the longer scales necessary...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nielson DM,Smith TA,Sreekumar V,Dennis S,Sederberg PB

更新日期：2015-09-01 00:00:00

abstract：:The calcium-dependent phosphatase calcineurin and its downstream transcriptional effector nuclear factor of activated T cells (NFAT) are important regulators of inducible gene expression in multiple cell types. In T cells, calcineurin-NFAT signaling represents a critical event for mediating cellular activation and the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bueno OF,Brandt EB,Rothenberg ME,Molkentin JD

更新日期：2002-07-09 00:00:00

abstract：:Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 X D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the sam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miskimins R,Ebato H,Seyfried TN,Yu RK

更新日期：1986-03-01 00:00:00

abstract：:Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) was found to increase the adherence of purified peripheral blood monocytes to plastic surfaces and to monolayers of human umbilical vein endothelial cells. With plastic surfaces as a model 9-hr culture with GM-CSF was necessary for enhancement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gamble JR,Elliott MJ,Jaipargas E,Lopez AF,Vadas MA

更新日期：1989-09-01 00:00:00

abstract：:Tissue factor (TF) plays an important role in hemostasis, inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer. In this article we uncover a mechanism in which protein S, which is well known as the cofactor of activated protein C, specifically inhibits TF activity by promoting the interact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hackeng TM,Seré KM,Tans G,Rosing J

更新日期：2006-02-28 00:00:00

abstract：:Health care is a crucial factor in US economic growth, because growing health care costs have made US corporations less competitive than their counterparts in countries where central governments assume most of those costs. In this paper we illustrate a second, possibly more powerful, effect of health care expenditures...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manton KG,Gu XL,Ullian A,Tolley HD,Headen AE Jr,Lowrimore G

更新日期：2009-12-15 00:00:00

abstract：:RNA-dependent RNA polymerases (RdRps) of the Nidovirales (Coronaviridae, Arteriviridae, and 12 other families) are linked to an amino-terminal (N-terminal) domain, called NiRAN, in a nonstructural protein (nsp) that is released from polyprotein 1ab by the viral main protease (Mpro). Previously, self-GMPylation/UMPylat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slanina H,Madhugiri R,Bylapudi G,Schultheiß K,Karl N,Gulyaeva A,Gorbalenya AE,Linne U,Ziebuhr J

更新日期：2021-02-09 00:00:00