Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase, an enzyme in ethylene biosynthesis.

abstract：:We performed a genome-wide analysis of gene expression in primary human CD15(+) myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee S,Zhou G,Clark T,Chen J,Rowley JD,Wang SM

更新日期：2001-03-13 00:00:00

abstract：:Malaria infection triggers vigorous host immune responses; however, the parasite ligands, host receptors, and the signaling pathways responsible for these reactions remain unknown or controversial. Malaria parasites primarily reside within RBCs, thereby hiding themselves from direct contact and recognition by host imm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Tian L,Yu X,Pattaradilokrat S,Li J,Wang M,Yu W,Qi Y,Zeituni AE,Nair SC,Crampton SP,Orandle MS,Bolland SM,Qi CF,Long CA,Myers TG,Coligan JE,Wang R,Su XZ

更新日期：2014-01-28 00:00:00

abstract：:The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)H]4-(N-maleimido)-benzyltrimethylammon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin A,Cowburn D

更新日期：1973-12-01 00:00:00

abstract：:Degeneracy, the ability of elements that are structurally different to perform the same function or yield the same output, is a well known characteristic of the genetic code and immune systems. Here, we point out that degeneracy is a ubiquitous biological property and argue that it is a feature of complexity at geneti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edelman GM,Gally JA

更新日期：2001-11-20 00:00:00

abstract：:The ribonucleotide reductase system of Escherichia coli B participates in the biosynthesis of DNA by reducing ribonucleoside diphosphates to the corresponding deoxyribonucleotides. The enzyme is regulated in a complicated way by allosteric modifiers. We now find that infection of E. coli with the bacteriophage T4 resu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berglund O,Karlström O,Reichard P

更新日期：1969-03-01 00:00:00

abstract：:Brood parasitism as an alternative female breeding tactic is particularly common in ducks, where hosts often receive eggs laid by parasitic females of the same species and raise their offspring. Herein, we test several aspects of a kin selection explanation for this phenomenon in goldeneye ducks (Bucephala clangula) b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andersson M,Ahlund M

更新日期：2000-11-21 00:00:00

abstract：:Covariance analysis of protein sequence alignments uses coevolving pairs of sequence positions to predict features of protein structure and function. However, current methods ignore the phylogenetic relationships between sequences, potentially corrupting the identification of covarying positions. Here, we use random m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin C,Colwell LJ

更新日期：2018-01-23 00:00:00

abstract：:We utilized the presence of hepatic receptors for galactose-terminal glycoproteins to achieve specific rescue of differentiated hepatocytes from methotrexate toxicity. This was accomplished by administration of a conjugate formed by covalent coupling of the methotrexate antagonist, folinic acid (leucovorin), to galact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu GY,Wu CH,Stockert RJ

更新日期：1983-05-01 00:00:00

abstract：:The endothelins (ETs) form a group of three vasoactive peptides (ET-1, ET-2, and ET-3) for which two types of cellular receptors have been identified, types A and B ET receptors (ETA and ETB receptors, respectively). To address possible targets for ETs within the liver, we isolated the four principal liver cell popula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Housset C,Rockey DC,Bissell DM

更新日期：1993-10-15 00:00:00

abstract：:Rett syndrome is a severe neurodevelopmental disorder caused by mutations in the methyl-CpG binding protein gene (MECP2). MeCP2 is a methyl-cytosine binding protein that is proposed to function as a transcriptional repressor. However, multiple gene expression studies comparing wild-type and MeCP2-deficient neurons hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kinde B,Wu DY,Greenberg ME,Gabel HW

更新日期：2016-12-27 00:00:00

abstract：:When the proximal and distal elements of wire-frame cubes are conflated, observers perceive illusory structures that no longer behave veridically. These phenomena suggest that what we normally see depends on visual associations generated by experience. The necessity of such learning may explain why the mammalian visua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Purves D,Andrews TJ

更新日期：1997-06-10 00:00:00

abstract：:Transarterial chemoembolization (TACE) is the main treatment for intermediate stage hepatocellular carcinoma (HCC) with Barcelona Clinic Liver Cancer classification because of its exclusive arterial blood supply. Although TACE achieves substantial necrosis of the tumor, complete tumor necrosis is uncommon, and the res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin WH,Yeh SH,Yeh KH,Chen KW,Cheng YW,Su TH,Jao P,Ni LC,Chen PJ,Chen DS

更新日期：2016-10-18 00:00:00

abstract：:Early passages of NIH 3T3 cells yield about 10 transformed foci for every 10(5) cells seeded after the cells multiply to confluence in a standardized 2-week assay. The question arose whether more cells would give rise to foci if given more time for their development. This question could not be answered simply by exten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chow M,Yao A,Rubin H

更新日期：1994-01-18 00:00:00

abstract：:Class I major histocompatibility complex (MHC) antigens are confronted with an apparently insurmountable dilemma. Each should show a binding preference to a common enough variety of nonapeptides, so that one relevant nonapeptide can be found in at least every other viral protein to provoke a cytotoxic T-cell response....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohno S

更新日期：1992-05-15 00:00:00

abstract：:Reproductive success is widely used as a measure of fitness. However, offspring quantity may not reflect the genetic contribution to subsequent generations if there is nonrandom variation in offspring quality. Offspring quality is likely to be an important component of human fitness, and tradeoffs between offspring qu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zietsch BP,Kuja-Halkola R,Walum H,Verweij KJ

更新日期：2014-01-21 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champeil P,Rigaud JL,Gary-Bobo CM

更新日期：1980-05-01 00:00:00

abstract：:Two deletion-substitution mutations of phage lambda, spi-113 and spi-274, are shown to remove about half of the rightward operator OR. Physiological studies show that spi-113 is still repressible. Thus, the 50 or so nucleotides of OR deleted in this mutant are not absolutely essential for repression. prm-116, which in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith GR,Eisen H,Reichardt L,Hedgepeth J

更新日期：1976-03-01 00:00:00

abstract：:The coordinated redistribution of sugars from mature "source" leaves to developing "sink" leaves requires tight regulation of sugar transport between cells via plasmodesmata (PD). Although fundamental to plant physiology, the mechanisms that control PD transport and thereby support development of new leaves have remai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brunkard JO,Xu M,Scarpin MR,Chatterjee S,Shemyakina EA,Goodman HM,Zambryski P

更新日期：2020-03-03 00:00:00

abstract：:Formamide is one of the important compounds from which prebiotic molecules can be synthesized, provided that its concentration is sufficiently high. For nucleotides and short DNA strands, it has been shown that a high degree of accumulation in hydrothermal pores occurs, so that temperature gradients might play a role ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niether D,Afanasenkau D,Dhont JK,Wiegand S

更新日期：2016-04-19 00:00:00

abstract：:Coherent anti-Stokes Raman scattering (CARS) spectra are reported for flavin adenine dinucleotide (FAD) and glucose oxidase (beta-D-glucose:oxygen 1-oxidoreductase; EC 1.1.3.4), in resonance with the 450 nm flavin absorption band. Several isoalloxazine ring modes were observed (1635, 1584, 1507, 1416, and 1359 cm(-1))...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dutta PK,Nestor JR,Spiro TG

更新日期：1977-10-01 00:00:00

abstract：:Growing knowledge of the key molecular components involved in biological processes such as endocytosis, exocytosis, and motility has enabled direct testing of proposed mechanistic models by reconstitution. However, current techniques for building increasingly complex cellular structures and functions from purified com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richmond DL,Schmid EM,Martens S,Stachowiak JC,Liska N,Fletcher DA

更新日期：2011-06-07 00:00:00

abstract：:In everyday listening, sound reaches our ears directly from a source as well as indirectly via reflections known as reverberation. Reverberation profoundly distorts the sound from a source, yet humans can both identify sound sources and distinguish environments from the resulting sound, via mechanisms that remain uncl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Traer J,McDermott JH

更新日期：2016-11-29 00:00:00

abstract：:Theory predicts that double-core-hole (DCH) spectroscopy can provide a new powerful means of differentiating between similar chemical systems with a sensitivity not hitherto possible. Although DCH ionization on a single site in molecules was recently measured with double- and single-photon absorption, double-core hole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berrah N,Fang L,Murphy B,Osipov T,Ueda K,Kukk E,Feifel R,van der Meulen P,Salen P,Schmidt HT,Thomas RD,Larsson M,Richter R,Prince KC,Bozek JD,Bostedt C,Wada S,Piancastelli MN,Tashiro M,Ehara M

更新日期：2011-10-11 00:00:00

abstract：:The location of renin (EC 3.4.99.19) in rat pituitary was determined by the peroxidase-antiperoxidase immunohistochemical technique. By using antisera prepared with purified rat renal renin, an immunoreactive substance was localized within ovoid cells scattered throughout the anterior pituitary. These cells were shown...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Naruse K,Takii Y,Inagami T

更新日期：1981-12-01 00:00:00

abstract：:The crystal structure of Escherichia coli ornithine transcarbamoylase (OTCase, EC 2.1.3.3) complexed with the bisubstrate analog N-(phosphonacetyl)-L-ornithine (PALO) has been determined at 2.8-A resolution. This research on the structure of a transcarbamoylase catalytic trimer with a substrate analog bound provides n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ha Y,McCann MT,Tuchman M,Allewell NM

更新日期：1997-09-02 00:00:00

abstract：:EF-hand Ca(II) binding sites share a conserved architecture and are prevalent in Ca(II) signaling pathways. The ion binding kinetics of these sites are carefully tuned to provide the physiologically appropriate activation and inactivation time scales. Here we examine kinetic tuning by the side chain at the ninth posit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renner M,Danielson MA,Falke JJ

更新日期：1993-07-15 00:00:00

abstract：:Leukocytes normally marginate toward the vascular wall in large vessels and within the microvasculature. Reversal of this process, leukocyte demargination, leads to substantial increases in the clinical white blood cell and granulocyte count and is a well-documented effect of glucocorticoid and catecholamine hormones,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fay ME,Myers DR,Kumar A,Turbyfield CT,Byler R,Crawford K,Mannino RG,Laohapant A,Tyburski EA,Sakurai Y,Rosenbluth MJ,Switz NA,Sulchek TA,Graham MD,Lam WA

更新日期：2016-02-23 00:00:00

abstract：:After more than two decades of study, many fundamental questions remain unanswered about the dynamics of glass-forming materials confined to thin films. Experiments and simulations indicate that free interfaces enhance dynamics over length scales larger than molecular sizes, and this effect strengthens at lower temper...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivancic RJS,Riggleman RA

更新日期：2020-10-13 00:00:00

abstract：:We report a machine-learning strategy for design of organic structure directing agents (OSDAs) for zeolite beta. We use machine learning to replace a computationally expensive molecular dynamics evaluation of the stabilization energy of the OSDA inside zeolite beta with a neural network prediction. We train the neural...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daeyaert F,Ye F,Deem MW

更新日期：2019-02-26 00:00:00

abstract：:Very-long-baseline interferometry images of the nuclear region of the radio galaxy Cygnus A reveal a pronounced "core" and a knotty jet and counterjet. The knots are moving away from the core at apparent speeds which are subluminal for h = 1 [h = H0/100 km.s-1.Mpc-1;1 parsec (pc) = 3.09 x 10(16)m] and about c for h = ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartel N,Sorathia B,Bietenholz MF,Carilli CL,Diamond P

更新日期：1995-12-05 00:00:00