Abstract:
:We performed a genome-wide analysis of gene expression in primary human CD15(+) myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. The correct genes were confirmed by application of the generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) technique for high-copy tags with multiple matches. A set of genes known to be important in myeloid differentiation were expressed at various levels and used different spliced forms. This study provides a normal baseline for comparison of gene expression in myeloid diseases. The strategy of using SAGE and GLGI techniques in this study has broad applications to the genome-wide identification of expressed genes.
journal_name
Proc Natl Acad Sci U S Aauthors
Lee S,Zhou G,Clark T,Chen J,Rowley JD,Wang SMdoi
10.1073/pnas.051013798keywords:
subject
Has Abstractpub_date
2001-03-13 00:00:00pages
3340-5issue
6eissn
0027-8424issn
1091-6490pii
98/6/3340journal_volume
98pub_type
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