Distinct quaternary structures of the AAA+ Lon protease control substrate degradation.

abstract：:An effective metabolism is essential to all living organisms, including the important human pathogen Staphylococcus aureus. To establish successful infection, S. aureus must scavenge nutrients and coordinate its metabolism for proliferation. Meanwhile, it also must produce an array of virulence factors to interfere wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ding Y,Liu X,Chen F,Di H,Xu B,Zhou L,Deng X,Wu M,Yang CG,Lan L

更新日期：2014-11-18 00:00:00

abstract：:The mammalian RNase A superfamily comprises a diverse array of ribonucleolytic proteins that have a variety of biochemical activities and physiological functions. Two rapidly evolving RNases of higher primates are of particular interest as they are major secretory proteins of eosinophilic leukocytes and have been foun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang J,Dyer KD,Rosenberg HF

更新日期：2000-04-25 00:00:00

abstract：:A near-full-length casbene synthase cDNA clone, pCS7, was isolated by using a partial cDNA clone, pCS4, to probe a lambda gt10 library constructed from poly(A)+ RNA from elicited castor bean seedlings. The cDNA insert had a length of 1983 bases with a polyadenylate tail of 19 bases. Translation of the cDNA sequence re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mau CJ,West CA

更新日期：1994-08-30 00:00:00

abstract：:Preparations of the hemin-controlled repressor (HCR) from rabbit reticulocytes contain 3':5'-cyclic-AMP-independent protein kinase activity for the smallest subunit of the peptide initiation factor eIF-2 and for proteins of reticulocyte 40S ribosomal subunits. Binding of the ternary complex formed between Met-tRNAf, G...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kramer G,Henderson AB,Pinphanichakarn P,Wallis MH,Hardesty B

更新日期：1977-04-01 00:00:00

abstract：:Rising sea levels and increased storminess are expected to accelerate the erosion of soft-cliff coastlines, threatening coastal infrastructure and livelihoods. To develop predictive models of future coastal change we need fundamentally to know how rapidly coasts have been eroding in the past, and to understand the dri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hurst MD,Rood DH,Ellis MA,Anderson RS,Dornbusch U

更新日期：2016-11-22 00:00:00

abstract：:Protein misfolding and the formation of aggregates are increasingly recognized components of the pathology of human genetic disease and hallmarks of many neurodegenerative disorders. As exemplified by polyglutamine diseases, the propensity for protein misfolding is associated with the length of polyglutamine expansion...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nollen EA,Garcia SM,van Haaften G,Kim S,Chavez A,Morimoto RI,Plasterk RH

更新日期：2004-04-27 00:00:00

abstract：:The day we understand the time evolution of subcellular events at a level of detail comparable to physical systems governed by Newton's laws of motion seems far away. Even so, quantitative approaches to cellular dynamics add to our understanding of cell biology. With data-guided frameworks we can develop better predic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ronquist S,Patterson G,Muir LA,Lindsly S,Chen H,Brown M,Wicha MS,Bloch A,Brockett R,Rajapakse I

更新日期：2017-11-07 00:00:00

abstract：:Despite the recognized role of tumor necrosis factor (TNF) in inflammation and neuronal degeneration, anti-TNF therapeutics failed to treat neurodegenerative diseases. Animal disease models had revealed the antithetic effects of the two TNF receptors (TNFR) in the central nervous system, whereby TNFR1 has been associa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong Y,Fischer R,Naudé PJ,Maier O,Nyakas C,Duffey M,Van der Zee EA,Dekens D,Douwenga W,Herrmann A,Guenzi E,Kontermann RE,Pfizenmaier K,Eisel UL

更新日期：2016-10-25 00:00:00

abstract：:A central question in developmental biology has been the initiation of cell-specific gene expression and its temporal relationship to morphogenesis. We have coupled embryo microdissection with the exquisite sensitivity of the polymerase chain reaction to define the onset of cell-specific gene expression during pancrea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gittes GK,Rutter WJ

更新日期：1992-02-01 00:00:00

abstract：:Gut microbial composition depends on different dietary habits just as health depends on microbial metabolism, but the association of microbiota with different diets in human populations has not yet been shown. In this work, we compared the fecal microbiota of European children (EU) and that of children from a rural Af...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Filippo C,Cavalieri D,Di Paola M,Ramazzotti M,Poullet JB,Massart S,Collini S,Pieraccini G,Lionetti P

更新日期：2010-08-17 00:00:00

abstract：:The "false thumb" of pandas is a carpal bone, the radial sesamoid, which has been enlarged and functions as an opposable thumb. If the giant panda (Ailuropoda melanoleuca) and the red panda (Ailurus fulgens) are not closely related, their sharing of this adaptation implies a remarkable convergence. The discovery of pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salesa MJ,Antón M,Peigné S,Morales J

更新日期：2006-01-10 00:00:00

abstract：:The local field at large distances from a charged or dipolar impurity in a nonpolar polarizable fluid is related to the dielectric constant of the medium. The predicted local field agrees accurately with molecular dynamics results. In contrast, the usual continuum theory prediction is only accurate to first order in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pollock EL,Alder BJ,Pratt LR

更新日期：1980-01-01 00:00:00

abstract：:The DNA-binding domain of c-Myb consists of three homologous tandem repeats of 52 amino acids. The structure of the third (C-terminal) repeat obtained by NMR analysis has a conformation related to the helix-turn-helix motif. To identify the role of each repeat in the sequence recognition of DNA, we analyzed specific i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanikawa J,Yasukawa T,Enari M,Ogata K,Nishimura Y,Ishii S,Sarai A

更新日期：1993-10-15 00:00:00

abstract：:In vitro DNA synthesis was studied in HeLa cell nuclei, with emphasis on the question of whether newly replicated DNA is associated with nucleosomes. The newly replicated DNA was twice as sensitive to digestion by micrococcal nuclease as mature chromatin DNA, reaching a limit digest at 20-25% acid-insoluble product. E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seale RL

更新日期：1978-06-01 00:00:00

abstract：:Patients with hematological malignancies can be successfully treated with HLA-matched T cell-depleted allogeneic stem cell transplantation (alloSCT) and subsequent donor lymphocyte infusions (DLIs). The efficacy of DLI is mediated by donor T cells recognizing minor histocompatibility antigens (mHags) on malignant reci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffioen M,van der Meijden ED,Slager EH,Honders MW,Rutten CE,van Luxemburg-Heijs SA,von dem Borne PA,van Rood JJ,Willemze R,Falkenburg JH

更新日期：2008-03-11 00:00:00

abstract：:CTCF sites are abundant in the genomes of diverse species but their function is enigmatic. We used chromosome conformation capture to determine long-range interactions among CTCF/cohesin sites over 2 Mb on human chromosome 11 encompassing the beta-globin locus and flanking olfactory receptor genes. Although CTCF occup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou C,Dale R,Dean A

更新日期：2010-02-23 00:00:00

abstract：:We identified cell surface markers associated with repression of p16(INK4a)/cyclin-dependent kinase inhibitor 2A(CDKN2A), a critical determinant in the acquisition of a plastic state. These cell surface markers allowed direct isolation of rare cells from healthy human breast tissue that exhibit extensive lineage plast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy S,Gascard P,Dumont N,Zhao J,Pan D,Petrie S,Margeta M,Tlsty TD

更新日期：2013-03-19 00:00:00

abstract：:By examining many alternative arrangements of cortical areas, we have found that the arrangement actually present in the brain minimizes the volume of the axons required for interconnecting the areas. Our observations support the notion that the organization of cortical areas has evolved to optimize interareal connect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klyachko VA,Stevens CF

更新日期：2003-06-24 00:00:00

abstract：:By using both double-colony hybridization and an in situ immunostaining assay for transformants, 39 cDNA clones (clone p-16 alpha) encoding mouse liver microsomal testosterone 16 alpha-hydroxylase (cytochrome P-450(16) alpha) were isolated from a cDNA library constructed in the cloning vector pUC-9 with poly(A)+ RNA i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harada N,Negishi M

更新日期：1985-04-01 00:00:00

abstract：:Diabetes is now the most common cause of progressive kidney failure leading to dialysis or transplantation. The central pathological feature of diabetic nephropathy is accumulation of extracellular matrix within the glomeruli. The factors in the diabetic milieu responsible for extracellular matrix accumulation are not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto T,Nakamura T,Noble NA,Ruoslahti E,Border WA

更新日期：1993-03-01 00:00:00

abstract：:To optimize the use of modified adenoviruses as vectors for gene delivery to the myocardium, we have characterized infection of cultured fetal and adult rat cardiac myocytes in vitro and of adult cardiac myocytes in vivo by using a replication-defective adenovirus carrying the chloramphenicol acetyltransferase (CAT) r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kass-Eisler A,Falck-Pedersen E,Alvira M,Rivera J,Buttrick PM,Wittenberg BA,Cipriani L,Leinwand LA

更新日期：1993-12-15 00:00:00

abstract：:Temporal dynamics of urban warming have been extensively studied at the diurnal scale, but the impact of background climate on the observed seasonality of surface urban heat islands (SUHIs) remains largely unexplored. On seasonal time scales, the intensity of urban-rural surface temperature differences ([Formula: see ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manoli G,Fatichi S,Bou-Zeid E,Katul GG

更新日期：2020-03-31 00:00:00

abstract：:In the last 60,000 y humans have expanded across the globe and now occupy a wider range than any other terrestrial species. Our ability to successfully adapt to such a diverse range of habitats is often explained in terms of our cognitive ability. Humans have relatively bigger brains and more computing power than othe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyd R,Richerson PJ,Henrich J

更新日期：2011-06-28 00:00:00

abstract：:A DNA-dependent RNA polymerase has been purified from disrupted virions of the Escherichia coli bacteriophage N4. The RNA polymerase is phage-coded and is required for class I N4 RNA synthesis, which is defined as RNA synthesized in vivo in the absence of post-infection protein synthesis. A polypeptide of molecular we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Falco SC,Zivin R,Rothman-Denes LB

更新日期：1978-07-01 00:00:00

abstract：:The his4 region of yeast contains the information necessary for the catalysis of three steps in the histidine biosynthetic pathway. The three activities specified by this region remain physically associated during gel filtration and ultracentrifugation and after extensive purification. Nonsense mutations in the "opera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaffer B,Rytka J,Fink GR

更新日期：1969-08-01 00:00:00

abstract：:Strong, short-ranged positional correlations involving counterions can induce a net attractive force between negatively charged strands of DNA and lead to the formation of ion pairs in dilute ionic solutions. However, the long range of the Coulomb interactions impedes the development of a simple local picture. We addr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YG,Weeks JD

更新日期：2006-05-16 00:00:00

abstract：:The endogenous cannabinoid anandamide is removed from the synaptic space by a high-affinity transport system present in neurons and astrocytes, which is inhibited by N-(4-hydroxyphenyl)-arachidonamide (AM404). After internalization, anandamide is hydrolyzed by fatty-acid amide hydrolase (FAAH), an intracellular membra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fegley D,Kathuria S,Mercier R,Li C,Goutopoulos A,Makriyannis A,Piomelli D

更新日期：2004-06-08 00:00:00

abstract：:Recently, several proteins have been identified that are related in their sequence to the p53 tumor-suppressor protein. One of these proteins, which is termed p73, exhibits sequence homology to the p53 transcriptional activation, DNA binding, and oligomerization domains. The adenovirus E1B 55-kDa protein, the adenovir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higashino F,Pipas JM,Shenk T

更新日期：1998-12-22 00:00:00

abstract：:Under anesthesia with sodium pentobarbital, the sciatic nerves of rats were transected bilaterally, and a catheter was inserted into the central end of the left renal artery. After an initial flush, an Alzet pump was attached to the catheter, containing various concentrations of glycyl-L-glutamine (Gly-Gln), methylpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koelle GB,Han MS

更新日期：1990-12-01 00:00:00

abstract：:Bone mineralization is an essential step during the embryonic development of vertebrates, and bone serves vital functions in human physiology. To systematically identify unique gene functions essential for osteogenesis, we performed a forward genetic screen in zebrafish and isolated a mutant, no bone (nob), that does ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huitema LF,Apschner A,Logister I,Spoorendonk KM,Bussmann J,Hammond CL,Schulte-Merker S

更新日期：2012-12-26 00:00:00