Abstract：
:This review briefly summarizes the single cell application of classical chemical dyes used to visualize cardiomyocyte physiology and their undesirable toxicities which have the potential to confound experimental observations. We will discuss, in detail, the more recent iterative development of fluorescent and bioluminescent protein-based indicators and their emerging application to cardiomyocytes. We will discuss the integration of optical control strategies (optogenetics) to augment the standard imaging approach. This will be done in the context of potential applications, and barriers, of these technologies to disease modelling, drug toxicity, and drug discovery efforts at the single-cell scale.
journal_name
Cellsjournal_title
Cellsauthors
Broyles CN,Robinson P,Daniels MJdoi
10.3390/cells7060051subject
Has Abstractpub_date
2018-05-31 00:00:00issue
6issn
2073-4409pii
cells7060051journal_volume
7pub_type
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