Abstract：
:Glioblastoma multiforme is one of the most malignant types of cancer. This is mainly due to a cell subpopulation with an extremely aggressive potential, called glioblastoma stem-like cells (GSCs). These cells produce high levels of extracellular adenosine which has been associated with increased chemoresistance, migration, and invasion in glioblastoma. In this study, we attempted to elucidate the mechanisms that control extracellular adenosine levels in GSC subtypes. By using primary and U87MG-derived GSCs, we associated increased extracellular adenosine with the mesenchymal phenotype. [3H]-adenosine uptake occurred mainly through the equilibrative nucleoside transporters (ENTs) in GSCs, but mesenchymal GSCs have lower expression and ENT1-mediated uptake activity than proneural GSCs. By analyzing expression and enzymatic activity, we determined that ecto-5'-nucleotidase (CD73) is predominantly expressed in proneural GSCs, driving AMPase activity. While in mesenchymal GSCs, both CD73 and Prostatic Acid Phosphatase (PAP) contribute to the AMP (adenosine monophosphate) hydrolysis. We did not observe significant differences between the expression of proteins involved in the metabolization of adenosine among the GCSs subtypes. In conclusion, the lower expression and activity of the ENT1 transporter in mesenchymal GSCs contributes to the high level of extracellular adenosine that these GSCs present.
journal_name
Cellsjournal_title
Cellsauthors
Alarcón S,Toro MLÁ,Villarreal C,Melo R,Fernández R,Ayuso Sacido A,Uribe D,San Martín R,Quezada Cdoi
10.3390/cells9081914subject
Has Abstractpub_date
2020-08-18 00:00:00issue
8issn
2073-4409pii
cells9081914journal_volume
9pub_type
杂志文章相关文献
Cells文献大全abstract：:ELISpot is one of the most commonly used immune monitoring assays, which allows the functional assessment of the immune system at the single cell level. With its outstanding sensitivity and ease of performance, the assay has recently advanced from the mere single function cell analysis to multifunctional analysis by i...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells3041102
更新日期：2014-11-27 00:00:00
abstract：:Myeloid cells, including macrophages and dendritic cells, represent an important first line of defense against infections. Upon recognition of pathogens, these cells undergo a metabolic reprogramming that supports their activation and ability to respond to the invading pathogens. An important metabolic regulator of th...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9010161
更新日期：2020-01-09 00:00:00
abstract：:Glioblastoma (GBM) is one of the most intransigent and aggressive brain tumors, and its treatment is extremely challenging and ineffective. To improve patients' expectancy and quality of life, new therapeutic approaches were investigated. Melatonin is an endogenous indoleamine with an incredible variety of properties....
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9030599
更新日期：2020-03-03 00:00:00
abstract：:Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural decellularized allo- or...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells1041010
更新日期：2012-11-05 00:00:00
abstract：:CCN1 and CCN2 are members of the CCN family and play essential roles in the regulation of multiple female reproductive functions, including ovulation. Cyclooxygenase-2 (COX2) is a critical mediator of ovulation and can be induced by sphingosine-1-phosphate (S1P) through the S1P1/3-mediated Yes-associated protein (YAP)...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8111445
更新日期：2019-11-15 00:00:00
abstract：:While females are less affected by non-diabetic kidney diseases compared to males, available data on sex differences in diabetic nephropathy (DN) are controversial. Although there is evidence for an imbalance of sex hormones in diabetes and hormone-dependent mechanisms in transforming growth factor β1 (TGF-β1) signali...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9102236
更新日期：2020-10-03 00:00:00
abstract：:Preeclampsia (PE) is a pregnancy-specific disorder associated with hypertension and proteinuria. Since there is no proven method to treat PE, early prediction and accurate diagnosis are essential for appropriate management of the disease. Thus, reliable biomarkers for diagnosing PE need to be identified and evaluated....
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9092003
更新日期：2020-08-31 00:00:00
abstract：:Parkinson's disease (PD) is currently the world's fastest-growing neurological disorder. It is characterized by motor and non-motor symptoms which progressively lead to significant clinical impairment, causing a high burden of disease. In addition to pharmacological therapies, various non-pharmacological treatment opt...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8020151
更新日期：2019-02-11 00:00:00
abstract：:We present a hiPSC-based 3D in vitro system suitable to test neurotoxicity (NT). Human iPSCs-derived 3D neurospheres grown in 96-well plate format were characterized timewise for 6-weeks. Changes in complexity and homogeneity were followed by immunocytochemistry and transmission electron microscopy. Transcriptional ac...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9051122
更新日期：2020-05-01 00:00:00
abstract：:Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have histor...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9040974
更新日期：2020-04-15 00:00:00
abstract：:The bioactive lipid sphingosine-1-phosphate (S1P), along with its receptors, modulates lymphocyte trafficking and immune responses to regulate skin inflammation. Macrophages are important in the pathogenesis of psoriasiform skin inflammation and express various S1P receptors. How they respond to S1P in skin inflammati...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8080785
更新日期：2019-07-28 00:00:00
abstract：:Aging is characterized by the gradual loss of tissue function and integrity. Activation of inflammatory responses accelerates the deterioration of cells and tissues. Many studies have shown that alteration of the components of the nuclear lamina is associated with inflammation, both in vivo and in vitro. However, the ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9030718
更新日期：2020-03-14 00:00:00
abstract：:Skin models with efficient skin barrier function are required for percutaneous absorption studies. The contribution of media supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) to the development of the skin barrier function of in vitro skin models remains incompletely understood. To investigate wheth...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8101142
更新日期：2019-09-25 00:00:00
abstract：:Despite major advances in the last 10 years, whether in terms of prevention or treatment, the 5 year survival rate remains relatively low for a large number of cancers. These therapeutic failures can be the consequence of several factors associated with the cellular modifications or with the host by itself, especially...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9020362
更新日期：2020-02-04 00:00:00
abstract：:Dysregulation of receptor tyrosine kinase-induced pathways is a critical step driving the oncogenic potential of brain cancer. In this study, we investigated the role of two members of the Sprouty (Spry) family in brain cancer-derived cell lines. Using immunoblot analyses we found essential differences in the pattern ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8080808
更新日期：2019-08-01 00:00:00
abstract：:More than 50% of human cancers harbor TP53 mutations and increased expression of Mouse double minute 2 homolog(MDM2), which contribute to cancer progression and drug resistance. Renal cell carcinoma (RCC) has an unusually high incidence of wild-type p53, with a mutation rate of less than 4%. MDM2 is master regulator o...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9061475
更新日期：2020-06-16 00:00:00
abstract：:Aquaporins (AQPs) facilitate transmembrane water and solute transport, and in addition to contributing to transepithelial water transport, they safeguard cell volume homeostasis. This study examined the expression and localization of AQP1 and AQP3 in the gills of Japanese medaka (Oryzias latipes) in response to osmoti...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8050422
更新日期：2019-05-08 00:00:00
abstract：:Retinal neurons, particularly retinal ganglion cells (RGCs), are susceptible to the degenerative damage caused by different inherited conditions and environmental insults, leading to irreversible vision loss and, ultimately, blindness. Numerous strategies are being tested in different models of degeneration to restore...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9081759
更新日期：2020-07-22 00:00:00
abstract：:Sustained endoplasmic reticular stress (ERS) is implicated in aggressive metastasis of cancer cells and increased tumor cell proliferation. Cancer cells activate the unfolded protein response (UPR), which aids in cellular survival and adaptation to harsh conditions. Inhibition of apoptosis, in contrast, is a mechanism...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells7100171
更新日期：2018-10-15 00:00:00
abstract：:Angiopoietins 1-4 (Ang1-4) represent an important family of growth factors, whose activities are mediated through the tyrosine kinase receptors, Tie1 and Tie2. The best characterized are angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2). Ang1 is a potent angiogenic growth factor signaling through Tie2, whereas Ang2 was ...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8050471
更新日期：2019-05-17 00:00:00
abstract：:Many receptors for neurotransmitters, such as dopamine, norepinephrine, acetylcholine, and neuropeptides, belong to the superfamily of G protein-coupled receptors (GPCRs). A general model posits that GPCRs undergo two-step homologous desensitization: the active receptor is phosphorylated by kinases of the G protein-co...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells10010052
更新日期：2020-12-31 00:00:00
abstract：:Metastasis is the leading cause of cancer death worldwide. Circulating tumor cells (CTCs) are a critical step in the metastatic cascade and a good tool to study this process. We isolated CTCs from a syngeneic mouse model of hepatocellular carcinoma (HCC) and a human xenograft mouse model of castration-resistant prosta...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8060618
更新日期：2019-06-20 00:00:00
abstract：:MicroRNAs (miRNAs) have emerged as key players in tumor angiogenesis. Interleukin-17C (IL-17C) was identified to promote colorectal cancer (CRC) progression. Therefore, we aimed to investigate the effect of IL-17C on tumor angiogenesis, the involvement of miR-23a-3p in IL-17C signaling, and the direct target gene of m...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9061363
更新日期：2020-06-01 00:00:00
abstract：:Tumors lack a well-regulated vascular supply of O2 and often fail to balance O2 supply and demand. Net O2 tension within many tumors may not only depend on O2 delivery but also depend strongly on O2 demand. Thus, tumor O2 consumption rates may influence tumor hypoxia up to true anoxia. Recent reports have shown that m...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121487
更新日期：2019-11-21 00:00:00
abstract：:Lysyl oxidase (LOX) proteins comprise a family of five copper-dependent enzymes (LOX and four LOX-like isoenzymes (LOXL1-4)) critical for extracellular matrix (ECM) homeostasis and remodeling. The primary role of LOX enzymes is to oxidize lysyl and hydroxylysyl residues from collagen and elastin chains into highly rea...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells8121483
更新日期：2019-11-21 00:00:00
abstract：:Nuclear lamins (NLs) are essential components of the animal cell nucleus involved in the regulation of a plethora of molecular and cellular processes. These include the nuclear envelope assembly and stability, mechanotransduction and chromatin organization, transcription, DNA replication, damage repair, and genomic in...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9061340
更新日期：2020-05-27 00:00:00
abstract：:T-cell acute lymphoblastic leukemia (T-ALL) is a rare, aggressive disease arising from T-cell precursors. NOTCH1 plays an important role both in T-cell development and leukemia progression, and more than 60% of human T-ALLs harbor mutations in components of the NOTCH1 signaling pathway, leading to deregulated cell gro...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9071726
更新日期：2020-07-18 00:00:00
abstract：:Differentiation therapy is an alternative strategy used to induce the differentiation of blast cells toward mature cells and to inhibit tumor cell proliferation for cancer treatment. Nobiletin (NOB), a polymethoxyflavone phytochemical, is present abundantly in citrus peels and has been reported to possess anti-cancer ...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells9040877
更新日期：2020-04-03 00:00:00
abstract：:(1) Background: Hyperglycemia leads to several biochemical and physiological consequences, such as the generation of advanced glycation end products (AGEs) and reactive oxygen species (ROS), which are involved in the development of several human diseases. Intestinal cells are continuously exposed to pro-oxidants and l...
journal_title：Cells
pub_type： 杂志文章
doi：10.3390/cells8121616
更新日期：2019-12-11 00:00:00
abstract：:Ferritin, the principal intracellular iron-storage protein localized in the cytoplasm, nucleus, and mitochondria, plays a major role in iron metabolism. The encoding ferritin genes are members of a multigene family that includes some pseudogenes. Even though pseudogenes have been initially considered as relics of anci...
journal_title：Cells
pub_type： 杂志文章,评审
doi：10.3390/cells9122554
更新日期：2020-11-28 00:00:00
