当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF PHARMACEUTICS期刊下所有文献 > Design of PEI-conjugated bio-reducible polymer for efficient gene delivery.

Design of PEI-conjugated bio-reducible polymer for efficient gene delivery.

Abstract：

:The poly(cystaminebis(acrylamide)-diaminohexane) (poly(CBA-DAH)) was designed previously as a bio-reducible efficient gene delivery carrier. However, the high weight ratio required to form the polyplexes between poly(CBA-DAH) with pDNA is still a problem that needs to be addressed. To solve this problem and increase the transfection efficiency, poly(ethylenimine) (PEI, 1.8 kDa) was conjugated to poly(CBA-DAH) via disulfide bond. The PEI conjugated poly(CBA-DAH) (PCDP) can bind with pDNA at a very low weight ratio of 0.5 and above, like PEI 25 kDa, and form the polyplexes with nano-size (102-128 nm) and positive surface charge (27-34 mV). PCDP and PCDP polyplexes had negligible cytotoxicity and indicated similar or better cellular uptake than the comparison groups such as PEI 25 kDa and Lipofectamine® polyplexes. To confirm the transfection efficiency, the plasmid DNA (pDNA) encoded with the luciferase reporter gene (gWiz-Luc) and green fluorescent protein reporter gene (GFP) were used and treated with PCDP into the A549, Huh-7, and Mia PaCa-2 cells. PCDP/pDNA polyplexes showed highest transfection efficiency in all tested cell lines. In the luciferase assay, PCDP polyplexes showed 10.2 times higher gene transfection efficiency than Lipofectamine® polyplexes in mimic in vivo conditions (30% FBS, A549 cells). The VEGF siRNA expressing plasmid (pshVEGF), which is constructed as a therapeutic gene by our previous work, was delivered by PCDP into the cancer cells. The VEGF gene expression of PCDP/pshVEGF polyplexes was dramatically lower than control and the VEGF gene silencing efficiencies of PCDP/pshVEGF (w/w; 10/1) polyplexes were 54% (A549 cells), 77% (Huh-7 cells), and 66% (Mia PaCa-2 cells). In addition, PCDP/pshVEGF had reduced cell viability rates of about 31% (A549 cells), 39% (Huh-7 cells), and 42% (Mia PaCa-2 cells) and showed better results than all comparison groups. In the transfection efficiency and VEGF silencing assay, PCDP polyplexes showed better results than poly(CBA-DAH) at 4-fold lower weight ratio. The data of all experiments demonstrate that the synthesized PCDP could be used for efficient gene delivery and could be widely applied.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Nam JP,Kim S,Kim SW

doi

10.1016/j.ijpharm.2018.04.051

subject

Has Abstract

pub_date

2018-07-10 00:00:00

pages

295-305

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378-5173(18)30279-5

journal_volume

545

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF PHARMACEUTICS文献大全
  • Preparation and investigation of core-shell nanoparticles containing human interferon-α.

    abstract：:Sustained release of active interferon-α (IFN-α) has been achieved from core-shell nanoparticles (NPs) prepared by aqueous precipitation of IFN-α-enriched human serum albumin (HSA-IFN-α) and layer-by-layer (L-b-L) by coating of the IFN-α NPs with poly(sodium-4-styrene) sulphonate (PSS) and chitosan (Chit). The concent...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.118825

    authors： Kristó K,Szekeres M,Makai Z,Márki Á,Kelemen A,Bali L,Pallai Z,Dékány I,Csóka I

    更新日期：2020-01-05 00:00:00

  • Study of branched cationic beta-cyclodextrin polymer/indomethacin complex and its release profile from alginate hydrogel.

    abstract：:A series of branched cationic beta-cyclodextrin polymers (CPbetaCDs) with designed chemical structures were synthesized from beta-cyclodextrin (beta-CD), epichlorohydrin (EP) and choline chloride (CC). Indomethacin (IDM), an anionic drug, was chosen as a model drug to evaluate the drug loading capacities of CPbetaCDs....

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.11.024

    authors： Xin J,Guo Z,Chen X,Jiang W,Li J,Li M

    更新日期：2010-02-15 00:00:00

  • The stimulated acoustic relaxation emission of maize starch tablets.

    abstract：:Stimulated acoustic relaxation emissions from maize starch tablets after compression were detected and recorded in the audible region. Stimulation was found to enhance the detected acoustic emission and to maintain recordable acoustic emission level longer. Based on the fact, that the stimulation by a halogen lamp and...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(01)00648-2

    authors： Mellin V,Salonen J,Laine E

    更新日期：2001-06-04 00:00:00

  • An intravenous application of magnetic nanoparticles for osteomyelitis treatment: An efficient alternative.

    abstract：:The infection of bone and bone marrow is called osteomyelitis. Treatment is difficult since antibiotics can not reach with enough concentration to the infected area. For the first time in this study, we have developed gentamicin-loaded magnetic gelatin nanoparticles (GMGNPs) for nanocarrier-mediated and magnetically t...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.119999

    authors： Ak G,Bozkaya ÜF,Yılmaz H,Sarı Turgut Ö,Bilgin İ,Tomruk C,Uyanıkgil Y,Hamarat Şanlıer Ş

    更新日期：2021-01-05 00:00:00

  • Tramadol sensing in non-invasive biological fluids using a voltammetric electronic tongue and an electrochemical sensor based on biomimetic recognition.

    abstract：:Tramadol (TRA) is a weak opioid analgesic, prescribed to relieve mild to moderately severe pain. However, side effects of TRA overdoses, including vomiting, depression, tachycardia, convulsions, morbidity and mortality are often reported. In this study, an electrochemical sensor based on molecularly imprinted conducti...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2020.120114

    authors： Diouf A,Aghoutane Y,Burhan H,Sen F,Bouchikhi B,El Bari N

    更新日期：2021-01-25 00:00:00

  • Critical evaluation of cadexomer-iodine ointment and povidone-iodine sugar ointment.

    abstract：:Topical iodine forms are used for infected and necrotic pressure ulcers. Despite antimicrobial advantages several potential disadvantages were observed with controversial results. To clarify the controversy, the reactivity of povidone-iodine (PI) sugar ointment and cadexomer-iodine (CI) ointment toward biological comp...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.01.007

    authors： Noda Y,Fujii K,Fujii S

    更新日期：2009-05-08 00:00:00

  • Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system.

    abstract：:Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer with two intramolecular disulphide bonds essential for its biological activity. A mutated form of murine IL-10 was successfully expressed in E. coli, recovered and purified from inclusion bodies. Its ability to reduc...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.08.040

    authors： Carvalho V,Castanheira P,Faria TQ,Gonçalves C,Madureira P,Faro C,Domingues L,Brito RM,Vilanova M,Gama M

    更新日期：2010-11-15 00:00:00

  • Ex vivo evaluation of degradation rates of metronidazole and olsalazine in distal ileum and in cecum: The impact of prandial state.

    abstract：PURPOSE:Evaluate ex vivo the bacterial metabolism induced degradation rates of mesalamine (negative control), metronidazole and olsalazine in distal ileum and in cecum. METHODS:The contents of distal ileum and cecum were collected during colonoscopy under anaerobic conditions from twelve healthy adults in the fasted a...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.10.015

    authors： Karatza E,Goumas C,Muenster U,Reppas C,Vertzoni M

    更新日期：2017-12-20 00:00:00

  • Study of Verapamil hydrochloride release from compressed hydrophilic Polyox-Wsr tablets.

    abstract：:This study deals with Verapamil hydrochloride release from tablets based on high molecular weight poly(ethylene oxide) (PEO). The drug release proceeds as a controlled diffusion (n=0.44-0.47), which rate is dependent on the molecular weight of PEO. Independent from it, under the conditions of the Half-change test, the...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00242-2

    authors： Dimitrov M,Lambov N

    更新日期：1999-10-28 00:00:00

  • Development of new polymer-based particulate systems for anti-glioma vaccination.

    abstract：:Biodegradable and biocompatible microspheres represent a promising alternative to conventional adjuvants for anti-tumour vaccination. Focusing on glioma, we developed two poly(D,L-lactide-co-glycolide) (PLGA)-based particulate systems presenting tumour antigens associated with plasma membranes or with cell lysates. Gl...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2005.10.046

    authors： Sapin A,Garcion E,Clavreul A,Lagarce F,Benoit JP,Menei P

    更新日期：2006-02-17 00:00:00

  • Synbiotic loaded chitosan-Ca-alginate microparticles reduces inflammation in the TNBS model of rat colitis.

    abstract：:New therapeutic strategies against inflammatory bowel disease (IBD) consider the usage of probiotics, prebiotics and synbiotics as beneficial for the intestinal microbial balance. Limitations of such an approach are addressed into difference in survival, persistence, colonization and variable effects among different p...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.05.049

    authors： Ivanovska TP,Mladenovska K,Zhivikj Z,Pavlova MJ,Gjurovski I,Ristoski T,Petrushevska-Tozi L

    更新日期：2017-07-15 00:00:00

  • A systematic study of captopril-loaded polyester fiber mats prepared by electrospinning.

    abstract：:In this study, drug-loaded nanofibers were prepared by electrospinning captopril (CPL) with aliphatic biodegradable polyesters. Poly(L-lactic acid) (PLLA), poly(lactic-co-glycolic acid) (PLGA), and poly(lactic-co-ε-caprolactone) (PLCL) were used as filament-forming matrix polymers, and the concentration of CPL in each...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.09.055

    authors： Zhang H,Lou S,Williams GR,Branford-White C,Nie H,Quan J,Zhu LM

    更新日期：2012-12-15 00:00:00

  • Alginate/chitosan microparticles for tamoxifen delivery to the lymphatic system.

    abstract：:Oral administration of the nonsteroidal anti-estrogen tamoxifen (TMX) is the treatment of choice for metastatic estrogen receptor-positive breast cancer. With the aim to improve TMX oral bioavailability and decrease its side effects, crosslinked alginate microparticles for the targeting to the lymphatic system by Peye...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.09.040

    authors： Coppi G,Iannuccelli V

    更新日期：2009-02-09 00:00:00

  • Entrapment and release of saquinavir using novel cationic solid lipid nanoparticles.

    abstract：:Cationic solid lipid nanoparticles (CSLNs) with entrapped saquinavir (SQV) were fabricated by microemulsion method. Here, CSLNs were stabilized by polysorbate 80, and the lipid phase contained cationic stearylamine (SA) and dioctadecyldimethyl ammonium bromide (DODAB) and nonionic Compritol 888 ATO (CA) and cacao butt...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.08.050

    authors： Kuo YC,Chen HH

    更新日期：2009-01-05 00:00:00

  • The influence of carbohydrate nature and drying methods on the compaction properties and pore structure of new methyl methacrylate copolymers.

    abstract：:Methyl methacrylate (MMA) copolymers have recently been proposed as an alternative in controlled-release matrix tablets. The aims of this study were to assess the potential value of these copolymers as direct compression excipients and to investigate relationships between the physical and structural properties of the ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(02)00432-5

    authors： Ferrero C,Jiménez-Castellanos MR

    更新日期：2002-11-06 00:00:00

  • Ionic liquids as ingredients in topical drug delivery systems.

    abstract：:Because of their properties, ionic liquids (ILs) (Ranke et al.) offer many advantages in topical drug delivery systems. For example, ionic liquids can be used to increase the solubility of sparingly soluble drugs and to enhance their topical and transdermal delivery. Furthermore, ILs can be used either to synthesize a...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.10.035

    authors： Dobler D,Schmidts T,Klingenhöfer I,Runkel F

    更新日期：2013-01-30 00:00:00

  • A new topical formulation for psoriasis: development of methotrexate-loaded nanostructured lipid carriers.

    abstract：:The aim of the present work was to develop and assess the potential of nanostructured lipid carriers (NLCs) loaded with methotrexate as a new approach for topical therapy of psoriasis. Methotrexate-loaded NLCs were prepared via a modified hot homogenization combined with ultrasonication techniques using either polysor...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2014.10.067

    authors： Pinto MF,Moura CC,Nunes C,Segundo MA,Costa Lima SA,Reis S

    更新日期：2014-12-30 00:00:00

  • Controllable encapsulation of α-mangostin with quaternized β-cyclodextrin grafted chitosan using high shear mixing.

    abstract：:In this study, the inclusion complex formation between α-mangostin and water-soluble quaternized β-CD grafted-chitosan (QCD-g-CS) was investigated. Inclusion complex formation with encapsulation efficiency (%EE) of 5, 15 and 75% can be varied using high speed homogenizer. Tuning %EE plays a role on physicochemical and...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.12.016

    authors： Phunpee S,Suktham K,Surassmo S,Jarussophon S,Rungnim C,Soottitantawat A,Puttipipatkhachorn S,Ruktanonchai UR

    更新日期：2018-03-01 00:00:00

  • Dissolution of fine particle fraction from truncated Anderson cascade impactor with an enhancer cell.

    abstract：:Dissolution testing for inhalers were previously conducted either on unfractionated drug-carrier powders or drug of specific aerodynamic particle size. In this study, the collection of the full fine particle fraction (FPF) was attempted on a single stage. Capsules containing 30 mg of 2% salbutamol sulfate (SS) was tes...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.04.048

    authors： Tay JYS,Liew CV,Heng PWS

    更新日期：2018-07-10 00:00:00

  • Enhanced oral delivery of alendronate by sucrose fatty acids esters in rats and their absorption-enhancing mechanisms.

    abstract：:Oral delivery is the most fascinating route for interminable drug remedy. However, the intestinal absorption of alendronate (ALN), a bisphosphonate drug after oral administration is very poor. Absorption enhancers, which help to achieve the efficiency-safety balance, are considered one of the most promising agents for...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2016.10.046

    authors： Alama T,Katayama H,Hirai S,Ono S,Kajiyama A,Kusamori K,Katsumi H,Sakane T,Yamamoto A

    更新日期：2016-12-30 00:00:00

  • Roll compaction process modeling: transfer between equipment and impact of process parameters.

    abstract：:In this study, the roll compaction of an intermediate drug load formulation was performed using horizontally and vertically force fed roll compactors. The horizontally fed roll compactor was equipped with an instrumented roll technology allowing the direct measurement of normal stress at the roll surface, while the ve...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.02.042

    authors： Souihi N,Reynolds G,Tajarobi P,Wikström H,Haeffler G,Josefson M,Trygg J

    更新日期：2015-04-30 00:00:00

  • Deoxycholate-hydrogels: novel drug carrier systems for topical use.

    abstract：:Na-deoxycholate (Na-DOC) forms a viscous thixotropic gel when in contact with excess buffer systems. The resulting gels have been tested as novel drug carrier systems for topical use. The influence of differing amounts of mannitol, glycerol and xylitol on the viscous modulus (G"/Pa) was evaluated by oscillatory measur...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(99)00170-2

    authors： Valenta C,Nowack E,Bernkop-Schnürch A

    更新日期：1999-08-05 00:00:00

  • Solid lipid nanoparticles as potential tools for gene therapy: in vivo protein expression after intravenous administration.

    abstract：:Naked plasmid DNA is a powerful tool for gene therapy, but it is rapidly eliminated from the circulation after intravenous administration. Therefore, the development of optimized DNA delivery systems is necessary for its successful clinical use. Solid lipid nanoparticles (SLNs) have demonstrated transfection capacity ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.10.020

    authors： del Pozo-Rodríguez A,Delgado D,Solinís MA,Pedraz JL,Echevarría E,Rodríguez JM,Gascón AR

    更新日期：2010-01-29 00:00:00

  • Nanoparticles of glycol chitosan and its thiolated derivative significantly improved the pulmonary delivery of calcitonin.

    abstract：:A novel thiomer derivative of glycol chitosan (GCS) was synthesized by coupling with thioglycolic acid (TGA) and evaluated for the pulmonary delivery of peptides. Nanoparticles (NPs) based on GCS and GCS-TGA were obtained by the ionic gelation method and demonstrated a particle size in the range of 0.23-0.33 microm wi...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.07.001

    authors： Makhlof A,Werle M,Tozuka Y,Takeuchi H

    更新日期：2010-09-15 00:00:00

  • Optimizing surfactant content to improve oral bioavailability of ibuprofen in microemulsions: just enough or more than enough?

    abstract：:Microemulsions show excellent potential as drug delivery systems, but the surfactants used to prepare them can cause side effects. Researchers have explored various strategies to expand microemulsion area and thereby reduce the surfactant content necessary, but how these strategies affect drug oral bioavailability has...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2014.05.031

    authors： You X,Xing Q,Tuo J,Song W,Zeng Y,Hu H

    更新日期：2014-08-25 00:00:00

  • Combination of time-dependent and pH-dependent polymethacrylates as a single coating formulation for colonic delivery of indomethacin pellets.

    abstract：:The objective of this study was to evaluate the combination of pH-dependent and time-dependent polymers as a single coating for design of colon delivery system of indomethacin pellets. Eudragit S100 and Eudragit L100 were used as pH-dependent polymers and Eudragit RS was used as a time-dependent polymer. A statistical...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2006.05.011

    authors： Akhgari A,Sadeghi F,Garekani HA

    更新日期：2006-08-31 00:00:00

  • An application of deep learning to detect process upset during pharmaceutical manufacturing using passive acoustic emissions.

    abstract：:The multivariate nature of a fluidized bed system creates process complexity that increases the risk of production upset. This research explores the use of passive acoustic emissions monitoring paired with an artificial neural network to detect fluidized bed distributor plate blockage. In many cases, early process fai...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.08.052

    authors： Carter A,Briens L

    更新日期：2018-12-01 00:00:00

  • Cyclodextrin-containing poly(ethyleneoxide) tablets for the delivery of poorly soluble drugs: potential as buccal delivery system.

    abstract：:The aim of this work was to develop a tablet for the buccal delivery of the poorly soluble drug carvedilol (CAR), based on poly(ethyleneoxide) (PEO) as bioadhesive sustained-release platform and hydroxypropyl-beta-cyclodextrin (HPbetaCD) as modulator of drug release. As first, PEO tablets loaded with CAR/HPbetaCD bina...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2006.03.031

    authors： Cappello B,De Rosa G,Giannini L,La Rotonda MI,Mensitieri G,Miro A,Quaglia F,Russo R

    更新日期：2006-08-17 00:00:00

  • Pharmacokinetics of Huperzine A after transdermal and oral administration in beagle dogs.

    abstract：:Comparison of single and multiple dose pharmacokinetics between patches and conventional tablets of Huperzine A (Hup-A) was performed in beagle dogs to evaluate the patches' controlled drug release characteristics in vivo, a newly developed transdermal system for treatment of Alzheimer disease. Results showed that tra...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.01.007

    authors： Ye JC,Zeng S,Zheng GL,Chen GS

    更新日期：2008-05-22 00:00:00

  • Determining the simultaneous presence of drug nanocrystals in drug-loaded polymeric nanocapsule aqueous suspensions: a relation between light scattering and drug content.

    abstract：:The encapsulation of lipophilic drugs in polymeric nanoparticles can form simultaneously both polymeric nanoparticles and drug nanocrystals. The objective was to detect the presence of nanocrystals in the nanoparticle suspensions using a simple methodology, and to determine if the nanocrystals are formed during prepar...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2008.04.007

    authors： Pohlmann AR,Mezzalira G,Venturini Cde G,Cruz L,Bernardi A,Jäger E,Battastini AM,da Silveira NP,Guterres SS

    更新日期：2008-07-09 00:00:00