当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF PHARMACEUTICS期刊下所有文献 > Solid lipid nanoparticles as potential tools for gene therapy: in vivo protein expression after intravenous administration.

Solid lipid nanoparticles as potential tools for gene therapy: in vivo protein expression after intravenous administration.

Abstract：

:Naked plasmid DNA is a powerful tool for gene therapy, but it is rapidly eliminated from the circulation after intravenous administration. Therefore, the development of optimized DNA delivery systems is necessary for its successful clinical use. Solid lipid nanoparticles (SLNs) have demonstrated transfection capacity in vitro, but their application for gene delivery has not been conveniently investigated in vivo. We aimed to evaluate the capacity of SLN-DNA vectors to transfect in vivo after intravenous administration to mice. The SLNs, composed of Precirol ATO 5, DOTAP and Tween 80 were complexed with the plasmid pCMS-EGFP which encodes the enhanced green fluorescent protein (EGFP). The resulting systems were characterized in vitro showing a mean particle size of 276 nm, superficial charge of +28 mV, the ability to protect the plasmid and transfection capacity in culture cells. The intravenous administration in mice led to transfection in hepatic tissue and spleen. Protein expression was detected from the third day after administration, and it was maintained for at least 1 week. This work shows for the first time the capacity of SLN-DNA vectors to induce the expression of a foreign protein after intravenous administration, supporting the potential of SLNs for gene therapy.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

del Pozo-Rodríguez A,Delgado D,Solinís MA,Pedraz JL,Echevarría E,Rodríguez JM,Gascón AR

doi

10.1016/j.ijpharm.2009.10.020

subject

Has Abstract

pub_date

2010-01-29 00:00:00

pages

157-62

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378-5173(09)00729-7

journal_volume

385

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF PHARMACEUTICS文献大全
  • A pharmaceutical study of doxorubicin-loaded PEGylated nanoparticles for magnetic drug targeting.

    abstract：:One of the new strategies to improve cancer chemotherapy is based on new drug delivery systems, like the polyethylene glycol-coated superparamagnetic iron oxide nanoparticles (PEG-SPION, thereafter called PS). In this study, PS are loaded with doxorubicin (DOX) anticancer drug, using a pre-formed DOX-Fe(2+) complex re...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.06.010

    authors： Gautier J,Munnier E,Paillard A,Hervé K,Douziech-Eyrolles L,Soucé M,Dubois P,Chourpa I

    更新日期：2012-02-14 00:00:00

  • Magnetic nanoparticles: In vivo cancer diagnosis and therapy.

    abstract：:Recently, significant research efforts have been devoted to the finding of efficient approaches in order to reduce the side effects of traditional cancer therapy and diagnosis. In this context, magnetic nanoparticles have attracted much attention because of their unique physical properties, magnetic susceptibility, bi...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijpharm.2015.07.059

    authors： Lima-Tenório MK,Pineda EA,Ahmad NM,Fessi H,Elaissari A

    更新日期：2015-09-30 00:00:00

  • Evaluation of collagen and methylated collagen as gene carriers.

    abstract：:This study explores the potential of DNA complexes prepared with methylated collagen (MC) and unmodified native collagen (NC) to deliver genes into cells. The physicochemical properties and transfection abilities of these two types of complexes are studied in parallel. MC was prepared by methylation of the carboxyl gr...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2004.04.014

    authors： Wang J,Lee IL,Lim WS,Chia SM,Yu H,Leong KW,Mao HQ

    更新日期：2004-07-26 00:00:00

  • Evaluation of childhood exposure to di(2-ethylhexyl) phthalate from perfusion kits during long-term parenteral nutrition.

    abstract：:Leachability of the plasticizer di(2-ethylhexyl) phthalate (DEHP) from administration sets into intravenous parenteral emulsions containing fat was investigated. DEHP is added to polyvinyl chloride (PVC) to impart flexibility. However, DEHP is a lipid-soluble suspected carcinogen that is hepatotoxic and teratogenic in...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(03)00335-1

    authors： Kambia K,Dine T,Gressier B,Bah S,Germe AF,Luyckx M,Brunet C,Michaud L,Gottrand F

    更新日期：2003-08-27 00:00:00

  • Chemical enhancer solubility in human stratum corneum lipids and enhancer mechanism of action on stratum corneum lipid domain.

    abstract：:Previously, chemical enhancer-induced permeation enhancement on human stratum corneum (SC) lipoidal pathway at enhancer thermodynamic activities approaching unity in the absence of cosolvents (defined as Emax) was determined and hypothesized to be related to the enhancer solubilities in the SC lipid domain. The object...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.09.014

    authors： Ibrahim SA,Li SK

    更新日期：2010-01-04 00:00:00

  • Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity.

    abstract：:The intranuclear disposition of plasmid DNA is highly important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on transgene expression were examined in COS-7 cells with two kinds of carriers (Lipofectamine Plus and TransIT-LT1). Three plasmids containing the curved ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.04.032

    authors： Kamiya H,Fukunaga S,Ohyama T,Harashima H

    更新日期：2009-07-06 00:00:00

  • In vitro and in vivo evaluation of a melamine dendrimer as a vehicle for drug delivery.

    abstract：:Cell-based and acute and subchronic in vivo toxicity profiles of a dendrimer based on melamine reveal that this class of molecules warrants additional study as vehicles for drug delivery. In cell culture, a substantial decrease in viability was observed at 0.1 mg/mL. For the acute studies, mice were administered 2.5, ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijpharm.2004.04.023

    authors： Neerman MF,Zhang W,Parrish AR,Simanek EE

    更新日期：2004-08-20 00:00:00

  • Interaction of gatifloxacin with efflux transporters: a possible mechanism for drug resistance.

    abstract：:The purpose of the study is to screen the interactions of fourth generation fluoroquinolone-gatifloxacin with efflux pumps, i.e., P-gp, MRP2 and BCRP. Mechanism of gatifloxacin interaction with efflux transporters may explain its acquired resistance. Such clarification may lead to the development of strategies to over...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.05.027

    authors： Kwatra D,Vadlapatla RK,Vadlapudi AD,Pal D,Mitra AK

    更新日期：2010-08-16 00:00:00

  • Dehydration behavior of nedocromil magnesium pentahydrate.

    abstract：:The dehydration of nedocromil magnesium (NM) pentahydrate proceeds in two steps, corresponding to the loss of four water molecules in the first step and one water molecule in the second step. The effects of temperature, particle size, sample weight, water vapor pressure and dehydration-rehydration cycle on both the ki...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(00)00700-6

    authors： Zhu H,Grant DJ

    更新日期：2001-03-14 00:00:00

  • Application of DoE approach in the development of mini-capsules, based on biopolymers and manuka honey polar fraction, as powder formulation for the treatment of skin ulcers.

    abstract：:The aim of the present work was the development of a powder formulation for the delivery of manuka honey (MH) bioactive components in the treatment of chronic skin ulcers. In particular pectin (PEC)/chitosan glutamate (CS)/hyaluronic acid (HA) mini-capsules were obtained by inverse ionotropic gelation in presence of c...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2016.10.050

    authors： Tenci M,Rossi S,Bonferoni MC,Sandri G,Mentori I,Boselli C,Cornaglia AI,Daglia M,Marchese A,Caramella C,Ferrari F

    更新日期：2017-01-10 00:00:00

  • Development of a PAT tool for monitoring the Wurster coater performance.

    abstract：:Real-time process analytical technology (PAT) is proposed as an effective approach for monitoring the performance of a Wurster coater. The coater was used for coating of 0.78 mm pharmaceutical pellets. The coating solution consisted of Hydroxypropyl methylcellulose/Poly ethylene glycol. During the coating process, sma...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.02.023

    authors： Foroughi-Dahr M,Sotudeh-Gharebagh R,Mostoufi N

    更新日期：2019-04-20 00:00:00

  • Formulation and in-vitro evaluation of directly compressed controlled release matrices of Losartan Potassium using Ethocel Grade 100 as rate retarding agent.

    abstract：:Current study was aimed to develop 200mg controlled release matrix tablets of Losartan Potassium using Ethocel 100 Premium and Ethocel 100 FP Premium as rate controlling polymer. In-vitro studies were performed according to USP Method-I in phosphate buffer (PH 6.8) using pharma test dissolution apparatus. The temperat...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.10.051

    authors： Khan KA,Khan GM,Zeeshan Danish M,Akhlaq,Khan H,Rehman F,Mehsud S

    更新日期：2015-12-30 00:00:00

  • Evaluation of the vibratory feeder method for assessment of powder flow properties.

    abstract：:The flow properties of pharmaceutical powders and blends used in solid oral dosage forms are an important consideration during dosage form development. The vibratory feeder method, a flow measurement technique that quantifies avalanche flow, has been adapted for measurement of the flow properties of common pharmaceuti...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2003.09.024

    authors： Bhattachar SN,Hedden DB,Olsofsky AM,Qu X,Hsieh WY,Canter KG

    更新日期：2004-01-28 00:00:00

  • Mechanical testing methods for drug-releasing vaginal rings.

    abstract：:Vaginal rings (VRs) are currently marketed for contraceptive or hormone regulation purposes, and investigationally, have been widely reported for delivery of antiretrovirals to reduce HIV transmission. To date, there is no national or international standard for the mechanical testing and minimum performance characteri...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2019.01.026

    authors： McCoy CF,Millar BG,Murphy DJ,Blanda W,Hansraj B,Devlin B,Malcolm RK,Boyd P

    更新日期：2019-03-25 00:00:00

  • Optimization of PLG microspheres for tailored drug release.

    abstract：:Here we explore the opportunity to design and then produce tailored release of therapeutic drugs from microcapsules. By use of "building blocks," formed from well characterized microcapsule populations, an inverse design algorithm has been developed that provides an optimal (in a least squares sense) combination of bu...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2009.09.010

    authors： Berchane NS,Jebrail FF,Andrews MJ

    更新日期：2010-01-04 00:00:00

  • Mechanical particle coating using ethylcellulose nanoparticle agglomerates for preparing controlled release fine particles; effect of coating temperature on coating performance.

    abstract：:This study describes the effect of coating temperature on the performance of mechanical particle coating using ethylcellulose, which was done to produce controlled-release particles (diameters less than 100 μm) with different release rates. First, theophylline crystals were spheronized using a mechanical powder proces...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.11.061

    authors： Kondo K,Ando C,Niwa T

    更新日期：2019-01-10 00:00:00

  • Slow-release of famotidine from tablets consisting of chitosan/sulfobutyl ether β-cyclodextrin composites.

    abstract：:An intermolecular complex formed from a 1:1 weight ratio of chitosan (CS, molecular weight 30 kDa) and sulfobutyl ether β-cyclodextrin (SBE-β-CyD, degree of substitution 7) was less soluble than either of the original components. The release of famotidine from tablets composed of a simple mixture of CS and SBE-β-CyD i...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2015.04.022

    authors： Anraku M,Hiraga A,Iohara D,Pipkin JD,Uekama K,Hirayama F

    更新日期：2015-06-20 00:00:00

  • Effect of divalent anions on photodegradation kinetics and pathways of riboflavin in aqueous solution.

    abstract：:The present investigation is based on a study of the effect of buffer and non-buffer divalent anions (phosphate, sulphate, tartrate, succinate, malonate) on the kinetics, product distribution and photodegradation pathways of riboflavin (RF) at pH 6.0-8.0. RF solutions (5x10(-5)M) were photodegraded in the presence of ...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2010.01.042

    authors： Ahmad I,Ahmed S,Sheraz MA,Vaid FH,Ansari IA

    更新日期：2010-05-10 00:00:00

  • Identification of molecular-interaction sites between lowly hydrolyzed polyvinyl alcohols and indomethacin by NMR spectroscopy.

    abstract：:To evaluate the potency of hydrolyzed polyvinyl alcohols (PVAs) as matrices for solid dispersion (SD) formulations, we examined their inhibitory effects on precipitation of a poorly water-soluble drug, indomethacin (IND). Additionally, to clarify their mechanism, we investigated the intermolecular interaction of IND w...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.08.018

    authors： Mori Y,Takahashi M,Ohno Y,Okura R,Ishida M,Higashi T,Motoyama K,Arima H

    更新日期：2018-10-05 00:00:00

  • α-Glucosyl hesperidin induced an improvement in the bioavailability of pranlukast hemihydrate using high-pressure homogenization.

    abstract：:The α-glucosyl hesperidin (Hsp-G)-induced improvement of both the dissolution and absorption properties of pranlukast hemihydrate (PLH) was achieved by means of a high-pressure homogenization (HPH) processing. The average particle size in the HPH-processed suspension was decreased significantly after 50 cycles of proc...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2011.03.017

    authors： Uchiyama H,Tozuka Y,Asamoto F,Takeuchi H

    更新日期：2011-05-30 00:00:00

  • Evolution of Cyclodextrin Nanosponges.

    abstract：:Cyclodextrin-based nanosponges (CD-NSs) are insoluble, highly cross-linked 3D network polymers used in several scientific and technological fields, the main area of investigation concerns the pharmaceutical applications, in which CD-NSs have been mostly employed as drug delivery systems. CD-NSs can be generally groupe...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijpharm.2017.06.072

    authors： Caldera F,Tannous M,Cavalli R,Zanetti M,Trotta F

    更新日期：2017-10-15 00:00:00

  • Discrete particle modeling and micromechanical characterization of bilayer tablet compaction.

    abstract：:A mechanistic particle scale model is proposed for bilayer tablet compaction. Making bilayer tablets involves the application of first layer compaction pressure on the first layer powder and a second layer compaction pressure on entire powder bed. The bonding formed between the first layer and the second layer particl...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2017.07.032

    authors： Yohannes B,Gonzalez M,Abebe A,Sprockel O,Nikfar F,Kiang S,Cuitiño AM

    更新日期：2017-08-30 00:00:00

  • Effects of carriers and storage of formulation on the lung deposition of a hydrophobic and hydrophilic drug from a DPI.

    abstract：:The effects of carriers, the drug:carrier ratio and a 1 month storage period of a formulation in permeable polystyrene tube at 40 degrees C/75% RH on the in vitro pulmonary deposition of model drugs from dry powder inhaler (DPI) were evaluated. Budesonide (hydrophobic) and salbutamol sulphate (hydrophilic) were used a...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/s0378-5173(03)00357-0

    authors： Harjunen P,Lankinen T,Salonen H,Lehto VP,Järvinen K

    更新日期：2003-09-16 00:00:00

  • Formulation optimization and topical delivery of quercetin from solid lipid based nanosystems.

    abstract：:The presence of large amounts of reactive oxygen species (ROS) leads to oxidative stress that can damage cell membranes, lead to DNA breakage and cause inactivation of free radical scavenger enzymes, eventually resulting in skin damage. Quercetin is a natural flavonoid that has been shown to have the highest anti-radi...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2012.12.013

    authors： Bose S,Du Y,Takhistov P,Michniak-Kohn B

    更新日期：2013-01-30 00:00:00

  • Gelatin-based membrane containing usnic acid-loaded liposome improves dermal burn healing in a porcine model.

    abstract：:There are a range of products available which claim to accelerate the healing of burns; these include topical agents, interactive dressings and biomembranes. The aim of this study was to assess the effect of a gelatin-based membrane containing usnic acid/liposomes on the healing of burns in comparison to silver sulfad...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2016.09.040

    authors： Nunes PS,Rabelo AS,Souza JC,Santana BV,da Silva TM,Serafini MR,Dos Passos Menezes P,Dos Santos Lima B,Cardoso JC,Alves JC,Frank LA,Guterres SS,Pohlmann AR,Pinheiro MS,de Albuquerque RL Júnior,Araújo AA

    更新日期：2016-11-20 00:00:00

  • Dissolution of fine particle fraction from truncated Anderson cascade impactor with an enhancer cell.

    abstract：:Dissolution testing for inhalers were previously conducted either on unfractionated drug-carrier powders or drug of specific aerodynamic particle size. In this study, the collection of the full fine particle fraction (FPF) was attempted on a single stage. Capsules containing 30 mg of 2% salbutamol sulfate (SS) was tes...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.04.048

    authors： Tay JYS,Liew CV,Heng PWS

    更新日期：2018-07-10 00:00:00

  • Manufacturing and characterization of long-acting levonorgestrel intrauterine systems.

    abstract：:Mirena® is long-acting (5 years) contraceptive intrauterine device. It is composed of a hollow cylindrical drug reservoir (containing Levonorgestrel and polydimethylsiloxane), which is covered with a release rate controlling silicone membrane. This structure presents a manufacturing challenge and to date, there have b...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.09.004

    authors： Bao Q,Gu B,Price CF,Zou Y,Wang Y,Kozak D,Choi S,Burgess DJ

    更新日期：2018-10-25 00:00:00

  • In-line quantification of two active ingredients in a batch blending process by near-infrared spectroscopy: influence of physical presentation of the sample.

    abstract：:The aim of this study was to apply near-infrared (NIR) spectroscopy to the simultaneous in-line monitoring of two active pharmaceutical ingredients (APIs) in a pharmaceutical batch blending process. The formulation under study consisted of a high load API (A1), one polymer, a second API (A2) and one lubricant. Additio...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2013.04.078

    authors： Martínez L,Peinado A,Liesum L

    更新日期：2013-07-15 00:00:00

  • A new topical formulation for psoriasis: development of methotrexate-loaded nanostructured lipid carriers.

    abstract：:The aim of the present work was to develop and assess the potential of nanostructured lipid carriers (NLCs) loaded with methotrexate as a new approach for topical therapy of psoriasis. Methotrexate-loaded NLCs were prepared via a modified hot homogenization combined with ultrasonication techniques using either polysor...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2014.10.067

    authors： Pinto MF,Moura CC,Nunes C,Segundo MA,Costa Lima SA,Reis S

    更新日期：2014-12-30 00:00:00

  • Impact of blend properties on die filling during tableting.

    abstract：:Based on characterization of a wide range of fillers and APIs, thirty divergent blends were composed and subsequently compressed on a rotary tablet press, varying paddle speed and turret speed. The tablet weight variability was determined of 20 grab samples consisting of each 20 tablets. Additionally, the bulk residen...

    journal_title：International journal of pharmaceutics

    pub_type： 杂志文章

    doi：10.1016/j.ijpharm.2018.08.015

    authors： Van Snick B,Grymonpré W,Dhondt J,Pandelaere K,Di Pretoro G,Remon JP,De Beer T,Vervaet C,Vanhoorne V

    更新日期：2018-10-05 00:00:00