Abstract：
:Optical measurement of membrane potentials enables fast, direct and simultaneous detection of membrane potentials from a population of neurons, providing a desirable approach for functional analysis of neuronal circuits. Here, we applied recently developed genetically encoded voltage indicators, ASAP1 (Accelerated Sensor of Action Potentials 1) and QuasAr2 (Quality superior to Arch 2), to zebrafish, an ideal model system for studying neurogenesis. To achieve this, we established transgenic lines which express the voltage sensors, and showed that ASAP1 is expressed in zebrafish neurons. To examine whether neuronal activity could be detected by ASAP1, we performed whole-cerebellum imaging, showing that depolarization was detected widely in the cerebellum and optic tectum upon electrical stimulation. Spontaneous activity in the spinal cord was also detected by ASAP1 imaging at single-cell resolution as well as at the neuronal population level. These responses mostly disappeared following treatment with tetrodotoxin, indicating that ASAP1 enabled optical measurement of neuronal activity in the zebrafish brain. Combining this method with other approaches, such as optogenetics and behavioural analysis may facilitate a deeper understanding of the functional organization of brain circuitry and its development.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Miyazawa H,Okumura K,Hiyoshi K,Maruyama K,Kakinuma H,Amo R,Okamoto H,Yamasu K,Tsuda Sdoi
10.1038/s41598-018-23906-1subject
Has Abstractpub_date
2018-04-16 00:00:00pages
6048issue
1issn
2045-2322pii
10.1038/s41598-018-23906-1journal_volume
8pub_type
杂志文章abstract：:Steep-slope transistors allow to scale down the supply voltage and the energy per computed bit of information as compared to conventional field-effect transistors (FETs), due to their sub-60 mV/decade subthreshold swing at room temperature. Currently pursued approaches to achieve such a subthermionic subthreshold swin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00359-6
更新日期：2017-03-23 00:00:00
abstract：:Respiratory syncytial virus (RSV) is a major cause of diseases of the respiratory tract in young children and babies, being mainly associated with bronchiolitis. RSV infection occurs primarily in pulmonary epithelial cells and, once infection is established, an immune response is triggered and neutrophils are recruite...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32576-y
更新日期：2018-09-21 00:00:00
abstract：:We compared the gut microbial populations in 100 women, from rural Ghana and urban US [50% lean (BMI < 25 kg/m2) and 50% obese (BMI ≥ 30 kg/m2)] to examine the ecological co-occurrence network topology of the gut microbiota as well as the relationship of short chain fatty acids (SCFAs) with obesity. Ghanaians consumed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35230-9
更新日期：2018-11-20 00:00:00
abstract：:Muscle specific receptor tyrosine kinase (MuSK) is an essential postsynaptic transmembrane molecule that mediates clustering of acetylcholine receptors (AChR). MUSK exon 10 is alternatively skipped in human, but not in mouse. Skipping of this exon disrupts a cysteine-rich region (Fz-CRD), which is essential for Wnt-me...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06841
更新日期：2014-10-30 00:00:00
abstract：:We propose to use Rabi oscillation as a probe to view the fractional Josepshon relation (FJR) associated with Majorana bound states (MBSs) expected in one-dimensional topological superconductors. The system consists of a quantum dot (QD) and an rf-SQUID with MBSs at the Josephson junction. Rabi oscillations between en...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11686
更新日期：2015-07-08 00:00:00
abstract：:High quality cadmium-free Zn-In-S:Ag doped-nanocrystals (d-NCs) were synthesized via a simple one-step noninjection route using silver nitrate, indium acetate, zinc acetate, oleylamine, S powder and 1-dodecanethiol as starting materials in an organic phase. The size and optical properties can be effectively tailored b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24459
更新日期：2016-04-14 00:00:00
abstract：:The aim is to analyse the clinical presentation, treatment and outcomes in patients with Paget's disease with invasive ductal carcinoma (PD-IDC), with special emphasis on the role of surgical treatment. Using data obtained by the Surveillance, Epidemiology, and End Results (SEER) program from 2010-2013, we investigate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45510
更新日期：2017-04-19 00:00:00
abstract：:We present a model on the simulation of the measurement-device independent quantum key distribution (MDI-QKD) with phase randomized general sources. It can be used to predict experimental observations of a MDI-QKD with linear channel loss, simulating corresponding values for the gains, the error rates in different bas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04612
更新日期：2014-04-14 00:00:00
abstract：:Sea buckthorn (Hippophae Rhamnoides L.) is an important source of bioactive compounds such as: antioxidants, vitamins, fatty acids, amino acids, and minerals. Sea buckthorn leaves extracts have been proved to have anti-microbial, antioxidant, anti-inflammatory, and anti-viral properties. In this paper, the extraction ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48610-6
更新日期：2019-08-19 00:00:00
abstract：:A Maxwell fisheye lens using parallel plate index grading is presented in this study to develop a passive retrodirective antenna array. As a proof-of-concept a design frequency of 10 GHz was selected for fabrication and experiment. The design principals of the lens are discussed, which enables 85% energy flow at the d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52779-1
更新日期：2019-11-07 00:00:00
abstract：:Human platelet antigens (HPAs) are membranous glycoproteins considered as alloantigens due to their polymorphisms. HPA-incompatibility in multiple pregnancies or blood transfusion can induce the development of alloantibodies leading to thrombocytopenia. The frequency of HPAs varies among populations, so that deep know...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64469-4
更新日期：2020-05-04 00:00:00
abstract：:Symbiodinium is best-known as the photosynthetic symbiont of corals, but some clades are symbiotic in other organisms or include free-living forms. Identifying similarities and differences among these clades can help us understand their relationship with corals, and thereby inform on measures to manage coral reefs in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15029-w
更新日期：2017-11-03 00:00:00
abstract：:Rab27 is an essential molecule of vesicle fusion and trafficking in exosome secretion process, which plays important roles in cancer progression and metastasis. Recent studies reported that Rab27 expression is also associated with cancer prognosis. Therefore, we performed a meta-analysis to reveal the prognostic signi...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-020-71104-9
更新日期：2020-08-24 00:00:00
abstract：:Targeting epigenetic reader proteins by small molecule inhibitors represents a new therapeutic concept in autoimmune diseases such as rheumatoid arthritis (RA). Although inhibitors targeting bromodomain protein 1 (BRD1) are in development, the function of BRD1 has hardly been studied. We investigated the therapeutic p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29127-w
更新日期：2018-07-24 00:00:00
abstract：:Mitochondrial genomes exhibit diverse features among eukaryotes in the aspect of gene content, genome structure, and the mobile genetic elements such as introns and plasmids. Although the number of published mitochondrial genomes is increasing at tremendous speed, those of several lineages remain unexplored. Here, we ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41238-6
更新日期：2019-03-19 00:00:00
abstract：:AKT is an important signal transduction protein that plays a crucial role in cancer development. Therefore, we evaluated associations between single nucleotide polymorphisms (SNPs) in the AKT promoter region and gastric cancer (GCa) risk in a case-control study of 1,110 GCa patients and 1,114 matched cancer-free contr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20008
更新日期：2016-01-28 00:00:00
abstract：:The serotonin transporter (SERT) is an integral membrane protein that exploits preexisting sodium-, chloride-, and potassium ion gradients to catalyze the thermodynamically unfavorable movement of synaptic serotonin into the presynaptic neuron. SERT has garnered significant clinical attention partly because it is the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23789
更新日期：2016-04-01 00:00:00
abstract：:Multi-domain voltage-gated ion channels appear to have evolved through sequential rounds of intragenic duplication from a primordial one-domain precursor. Whereas modularity within one-domain symmetrical channels is established, little is known about the roles of individual regions within more complex asymmetrical cha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38426
更新日期：2016-12-12 00:00:00
abstract：:Mechanistic exploration of the origins of the unparalleled soil microbial biodiversity represents a vast and uncharted scientific frontier. Quantification of candidate mechanisms that promote and sustain such diversity must be linked with microbial functions and measurable biophysical interactions at appropriate scale...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00881
更新日期：2012-01-01 00:00:00
abstract：:Cover crops (plants grown in an agricultural rotation between cash crops) can significantly improve soil quality via sequestering carbon, retaining nutrients, decreasing soil erosion, and maintaining belowground biodiversity. However, little is known about the effects of such plants upon soil structure. The aim of the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43937-6
更新日期：2019-05-16 00:00:00
abstract：:Depressive disorder (DD) is a mental illness affecting more than 300 million people worldwide, whereas social stigma and subtle, variant symptoms impede diagnosis. Psychomotor retardation is a common component of DD with a negative impact on motor function, usually reflected on patients' routine activities, including,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50002-9
更新日期：2019-09-16 00:00:00
abstract：:The tumor microenvironment (TME) plays critical roles in tumor growth and progression, however key regulators of gene expression in the TME of cutaneous malignant peripheral nerve sheath tumor (C-MPNST) and spindle cell melanoma (SCM) have not been well elucidated. Herein, we investigate the epigenetic regulation of p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69787-1
更新日期：2020-08-03 00:00:00
abstract：:Chondrichthyes - sharks, rays, skates, and chimeras, are among the most threatened and data deficient vertebrate species. Global demand for shark and ray derived products, drives unregulated and exploitative fishing practices, which are in turn facilitated by the lack of ecological data required for effective conserva...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40940-9
更新日期：2019-03-14 00:00:00
abstract：:Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25030
更新日期：2016-04-29 00:00:00
abstract：:We investigated whether dogs remember their spontaneous past actions relying on episodic-like memory. Dogs were trained to repeat a small set of actions upon request. Then we tested them on their ability to repeat other actions produced by themselves, including actions performed spontaneously in everyday situations. D...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67302-0
更新日期：2020-06-26 00:00:00
abstract：:Temporal precision is a determinant of performance in various motor activities. Although the accuracy and precision of timing in activities have been previously measured and quantified, temporal dynamics with flexible precision have not been considered. Here, we examined the temporal dynamics in timed motor activities...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76953-y
更新日期：2020-11-18 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) plays an important role in aggravating invasiveness and metastatic behavior of colorectal cancer (CRC). Identification of EMT is important for structuring treatment strategy, but has not yet been studied by using noninvasive imaging modality. Diffusion kurtosis imaging (DKI) is ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11808-7
更新日期：2017-09-12 00:00:00
abstract：:Plant derived biogenic synthesis of nanoparticles (NP) has been the recent trend in material science as featured sustainable catalysts. A great deal of the current nanocatalytic research has been oriented on the bio-inspired green catalysts based on their wide applicability. In this context, CuO NPs are synthesized fo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81320-6
更新日期：2021-01-21 00:00:00
abstract：:The triethylamine promoted three-component reaction of N-(4-nitrobenzyl), N-ethoxycarbonylmethylisoquinolinium bromide, isatins and malononitrile in ethanol afforded spiro[indoline-3,2'-pyrrolo[2,1-a]isoquinolines] in good yields and with high diastereoselectivity. The similar reaction of N-cyanomethylisoquinolinium c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41024
更新日期：2017-01-20 00:00:00
abstract：:Circular RNAs (circRNAs) are abundantly expressed in the haematopoietic compartment, but knowledge on their diversity among blood cell types is still limited. Nevertheless, emerging data indicate an array of circRNA functions exerted through interactions with other RNAs and proteins, by translation into peptides, and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50864-z
更新日期：2019-10-11 00:00:00
