当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Involvement of AMP-activated protein kinase and Death Receptor 5 in TRAIL-Berberine-induced apoptosis of cancer cells.

Involvement of AMP-activated protein kinase and Death Receptor 5 in TRAIL-Berberine-induced apoptosis of cancer cells.

Abstract：

:Our previous studies indicated that combination of Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and PPARγ ligand Troglitazone (TZD), can induce significant apoptosis in various TRAIL-resistant prostate and hepatocellular carcinoma (HCC) cells. These also suggested serine/threonine kinase AMP-activated protein kinase (AMPK) to be a mediator of TRAIL-TZD-induced apoptosis. To further validate AMPK's role in TRAIL sensitization, we determined the apoptotic potential of TRAIL in combination with the natural compound Berberine (BBR), the latter being a potent activator of AMPK. These demonstrated a significant reduction of cell viability and induction of apoptosis (increased cleavage of caspase 3, 8, 9) when treated with TRAIL-BBR combination. This apoptosis is attenuated in cells overexpressing AMPKα-dominant negative (DN) or following AMPKα knockdown, confirming involvement of AMPK. To identify potential downstream mediators involved, an apoptosis RT2 PCR array analysis was performed. These showed induction of several genes including TNFRSF10B (expresses DR5) and Harakiri following BBR treatment, which were further validated by qPCR analysis. Furthermore, knocking down DR5 expression significantly attenuated TRAIL-BBR-induced apoptosis, suggesting DR5 to be a mediator of this apoptosis. Our studies indicate that combination of TRAIL and AMPK activator BBR might be an effective means of ameliorating TRAIL-resistance involving DR5 in advanced cancer.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ke R,Vishnoi K,Viswakarma N,Santha S,Das S,Rana A,Rana B

doi

10.1038/s41598-018-23780-x

subject

Has Abstract

pub_date

2018-04-03 00:00:00

pages

5521

issue

1

issn

2045-2322

pii

10.1038/s41598-018-23780-x

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Author Correction: Ultra high dose rate (35 Gy/sec) radiation does not spare the normal tissue in cardiac and splenic models of lymphopenia and gastrointestinal syndrome.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-67913-7

    authors： Venkatesulu BP,Sharma A,Pollard-Larkin JM,Sadagopan R,Symons J,Neri S,Singh PK,Tailor R,Lin SH,Krishnan S

    更新日期：2020-06-30 00:00:00

  • Ultrasonography of wallaby prenatal development shows that the climb to the pouch begins in utero.

    abstract：:Marsupials have a functional placenta for a shorter period of time compared to that of eutherian species, and their altricial young reach the teats without any help from the mother. We have monitored the short intrauterine development of one marsupial, the tammar wallaby, with high-resolution ultrasound from reactivat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01458

    authors： Drews B,Roellig K,Menzies BR,Shaw G,Buentjen I,Herbert CA,Hildebrandt TB,Renfree MB

    更新日期：2013-01-01 00:00:00

  • Mito-oncology agent: fermented extract suppresses the Warburg effect, restores oxidative mitochondrial activity, and inhibits in vivo tumor growth.

    abstract：:Mitochondrial dysfunction and significant changes in metabolic pathways accompany cancer development and are responsible for maintaining the tumor microenvironment. Normal mitochondria can trigger intrinsic apoptosis by releasing cytochrome c into the cytosol. The survival of malignant cells highly depends on the supp...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71118-3

    authors： Bencze G,Bencze S,Rivera KD,Watson JD,Hidvegi M,Orfi L,Tonks NK,Pappin DJ

    更新日期：2020-08-25 00:00:00

  • Determining correlates of the average number of cigarette smoking among college students using count regression models.

    abstract：:College students, as a large part of young adults, are a vulnerable group to several risky behaviors including smoking and drug abuse. This study aimed to utilize and to compare count regression models to identify correlates of cigarette smoking among college students. This was a cross-sectional study conducted on stu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-65813-4

    authors： Sharareh P,Leili T,Abbas M,Jalal P,Ali G

    更新日期：2020-06-01 00:00:00

  • SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner.

    abstract：:Neurotransmitter release is mediated by the SNARE complex, but the role of its phosphorylation has scarcely been elucidated. Although PKC activators are known to facilitate synaptic transmission, there has been a heated debate on whether PKC mediates facilitation of neurotransmitter release through phosphorylation. On...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08237-x

    authors： Katayama N,Yamamori S,Fukaya M,Kobayashi S,Watanabe M,Takahashi M,Manabe T

    更新日期：2017-08-11 00:00:00

  • Physical Activity, Energy Expenditure, and Defense of Body Weight in Melanocortin 4 Receptor-Deficient Male Rats.

    abstract：:Melanocortin 4 receptor (MC4R) variants contribute to human obesity, and rats lacking functional MC4R (Mc4rK314X/K314X) are obese. We investigated the hypothesis that low energy expenditure (EE) and physical activity contribute to this obese phenotype in male rats, and determined whether lack of functional MC4R confer...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37435

    authors： Almundarij TI,Smyers ME,Spriggs A,Heemstra LA,Beltz L,Dyne E,Ridenour C,Novak CM

    更新日期：2016-11-25 00:00:00

  • Machine Learning Approach to find the relation between Endometriosis, benign breast disease, cystitis and non-toxic goiter.

    abstract：:The exact mechanism of endometriosis is unknown. The recommendation system (RS) based on item similarities of machine learning has never been applied to the relationship between diseases. The study aim was to identify diseases associated with endometriosis by applying RS based on item similarities to insurance data in...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41973-w

    authors： Lee JH,Kwon SY,Chang J,Yuk JS

    更新日期：2019-04-01 00:00:00

  • Study on dose-dependent, frequency-dependent, and accumulative effects of 1.5 GHz and 2.856 GHz microwave on cognitive functions in Wistar rats.

    abstract：:Many studies have revealed the cognitive decline induced by microwave radiation. However, the systematic study on dose-dependent, frequency-dependent and accumulative effects of microwave exposure at different frequencies was lacking. Here, we studied the relationship between the effects and the power and frequency of...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-11420-9

    authors： Tan S,Wang H,Xu X,Zhao L,Zhang J,Dong J,Yao B,Wang H,Zhou H,Gao Y,Peng R

    更新日期：2017-09-07 00:00:00

  • Functional Genome Screening to Elucidate the Colistin Resistance Mechanism.

    abstract：:Antibiogram profile of 1590 clinical bacterial isolates based on thirteen different antimicrobial compounds showed that 1.6% of the bacterial isolates are multidrug resistant. Distribution pattern based on 16S rRNA sequence analysis showed that Pseudomonas aeruginosa constituted the largest group (83.6%) followed by B...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep23156

    authors： Kumar M,Gupta A,Sahoo RK,Jena J,Debata NK,Subudhi E

    更新日期：2016-03-18 00:00:00

  • Mitogenomics of historical type specimens of Australasian turtles: clarification of taxonomic confusion and old mitochondrial introgression.

    abstract：:Diagnosability is central to taxonomy as are type specimens which define taxa. New advances in technologies and the discovery of new informative traits must be matched with previous taxonomic decisions based on name-bearing type specimens. Consequently, the challenge of sequencing highly degraded DNA from historical t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-42310-x

    authors： Kehlmaier C,Zhang X,Georges A,Campbell PD,Thomson S,Fritz U

    更新日期：2019-04-09 00:00:00

  • Sensations from a single M-cone depend on the activity of surrounding S-cones.

    abstract：:Color vision requires the activity of cone photoreceptors to be compared in post-receptoral circuitry. Decades of psychophysical measurements have quantified the nature of these comparative interactions on a coarse scale. How such findings generalize to a cellular scale remains unclear. To answer that question, we qua...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-26754-1

    authors： Schmidt BP,Sabesan R,Tuten WS,Neitz J,Roorda A

    更新日期：2018-06-04 00:00:00

  • Pattern Discovery in Brain Imaging Genetics via SCCA Modeling with a Generic Non-convex Penalty.

    abstract：:Brain imaging genetics intends to uncover associations between genetic markers and neuroimaging quantitative traits. Sparse canonical correlation analysis (SCCA) can discover bi-multivariate associations and select relevant features, and is becoming popular in imaging genetic studies. The L1-norm function is not only ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-13930-y

    authors： Du L,Liu K,Yao X,Yan J,Risacher SL,Han J,Guo L,Saykin AJ,Shen L,Alzheimer’s Disease Neuroimaging Initiative.

    更新日期：2017-10-25 00:00:00

  • A national assessment of the epidemiology of severe fever with thrombocytopenia syndrome, China.

    abstract：:First discovered in rural areas of middle-eastern China in 2009, severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne zoonosis affecting hundreds of cases reported in China each year. Using the national surveillance data from 2010 to 2013, we conducted this retrospective epidemiological study a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep09679

    authors： Liu K,Zhou H,Sun RX,Yao HW,Li Y,Wang LP,Di Mu,Li XL,Yang Y,Gray GC,Cui N,Yin WW,Fang LQ,Yu HJ,Cao WC

    更新日期：2015-04-23 00:00:00

  • Identification and characterisation of lamprey protein kinase C delta-like gene.

    abstract：:Protein kinase C-δ (PKC-δ), a member of the lipid-regulated serine/threonine PKC family, has been implicated in a wide range of important cellular processes, such as cell growth, differentiation, and apoptosis. Lampreys belong to the most primitive class of vertebrates, and there is little information on PKC-δ in thes...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-12526-w

    authors： Xu Y,Zhu S,Zhao H,Li Q

    更新日期：2017-09-22 00:00:00

  • New insights on Pseudoalteromonas haloplanktis TAC125 genome organization and benchmarks of genome assembly applications using next and third generation sequencing technologies.

    abstract：:Pseudoalteromonas haloplanktis TAC125 is among the most commonly studied bacteria adapted to cold environments. Aside from its ecological relevance, P. haloplanktis has a potential use for biotechnological applications. Due to its importance, we decided to take advantage of next generation sequencing (Illumina) and th...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52832-z

    authors： Qi W,Colarusso A,Olombrada M,Parrilli E,Patrignani A,Tutino ML,Toll-Riera M

    更新日期：2019-11-11 00:00:00

  • Smoothelin-like 1 deletion enhances myogenic reactivity of mesenteric arteries with alterations in PKC and myosin phosphatase signaling.

    abstract：:The role of the smoothelin-like 1 (SMTNL1) protein in mediating vascular smooth muscle contractile responses to intraluminal pressure was examined in resistance vessels. Mesenteric arterioles from wild type (WT) and SMTNL1 global knock-out (KO) mice were examined with pressure myography. SMTNL1 deletion was associated...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36564-0

    authors： Turner SR,Chappellaz M,Popowich B,Wooldridge AA,Haystead TAJ,Cole WC,MacDonald JA

    更新日期：2019-01-24 00:00:00

  • Room Temperature Characteristics of Polymer-Based Low Ice Adhesion Surfaces.

    abstract：:Ice adhesion is mainly dictated by surface properties, and water wettability is frequently correlated with ice adhesion strength. However, these established correlations are limited to high ice adhesion and become invalid when the ice adhesion strength is low. Here we carried out an experimental study to explore the r...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42181

    authors： He Z,Vågenes ET,Delabahan C,He J,Zhang Z

    更新日期：2017-02-07 00:00:00

  • Author Correction: The endocannabinoid hydrolase FAAH is an allosteric enzyme.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-62514-w

    authors： Dainese E,Oddi S,Simonetti M,Sabatucci A,Angelucci CB,Ballone A,Dufrusine B,Fezza F,De Fabritiis G,Maccarrone M

    更新日期：2020-03-31 00:00:00

  • Functional association of cellular microtubules with viral capsid assembly supports efficient hepatitis B virus replication.

    abstract：:Viruses exploit host factors and environment for their efficient replication. The virus-host interaction mechanisms for achieving an optimal hepatitis B virus (HBV) replication have been largely unknown. Here, a single cell cloning revealed that HepAD38 cells, a widely-used HBV-inducible cell line, contain cell clones...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-11015-4

    authors： Iwamoto M,Cai D,Sugiyama M,Suzuki R,Aizaki H,Ryo A,Ohtani N,Tanaka Y,Mizokami M,Wakita T,Guo H,Watashi K

    更新日期：2017-09-06 00:00:00

  • Mode of renal replacement therapy determines endotoxemia and neutrophil dysfunction in chronic kidney disease.

    abstract：:Bacterial infection and sepsis are common complications of chronic kidney disease (CKD). A vicious cycle of increased gut permeability, endotoxemia, inadequate activation of the innate immune system and resulting innate immune dysfunction is hypothesized. We assessed endotoxemia, neutrophil function and its relation t...

    journal_title：Scientific reports

    pub_type： 临床试验,杂志文章

    doi：10.1038/srep34534

    authors： Lemesch S,Ribitsch W,Schilcher G,Spindelböck W,Hafner-Gießauf H,Marsche G,Pasterk L,Payerl D,Schmerböck B,Tawdrous M,Rosenkranz AR,Stiegler P,Kager G,Hallström S,Oettl K,Eberhard K,Horvath A,Leber B,Stadlbauer V

    更新日期：2016-10-04 00:00:00

  • Energetics of Endotoxin Recognition in the Toll-Like Receptor 4 Innate Immune Response.

    abstract：:Bacterial outer membrane lipopolysaccharide (LPS) potently stimulates the mammalian innate immune system, and can lead to sepsis, the primary cause of death from infections. LPS is sensed by Toll-like receptor 4 (TLR4) in complex with its lipid-binding coreceptor MD-2, but subtle structural variations in LPS can profo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep17997

    authors： Paramo T,Tomasio SM,Irvine KL,Bryant CE,Bond PJ

    更新日期：2015-12-09 00:00:00

  • Spectrally-selective all-inorganic scattering luminophores for solar energy-harvesting clear glass windows.

    abstract：:All-inorganic visibly-transparent energy-harvesting clear laminated glass windows are the most practical solution to boosting building-integrated photovoltaics (BIPV) energy outputs significantly while reducing cooling- and heating-related energy consumption in buildings. By incorporating luminophore materials into la...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep06632

    authors： Alghamedi R,Vasiliev M,Nur-E-Alam M,Alameh K

    更新日期：2014-10-16 00:00:00

  • Structural Modeling of Chromatin Integrates Genome Features and Reveals Chromosome Folding Principle.

    abstract：:How chromosomes fold into 3D structures and how genome functions are affected or even controlled by their spatial organization remain challenging questions. Hi-C experiment has provided important structural insights for chromosome, and Hi-C data are used here to construct the 3D chromatin structure which are character...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02923-6

    authors： Xie WJ,Meng L,Liu S,Zhang L,Cai X,Gao YQ

    更新日期：2017-06-06 00:00:00

  • An understanding of third-party friendships in a tolerant macaque.

    abstract：:Complex societies are shaped by social relationships between multiple individuals. The pressure to track these relationships has driven the evolution of social cognition in primates. Importantly, it can be adaptive to track not only personal relationships, but also those established between third-parties. Primates hav...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66407-w

    authors： Whitehouse J,Meunier H

    更新日期：2020-06-17 00:00:00

  • Multidecade Mortality and a Homolog of Hepatitis C Virus in Bald Eagles (Haliaeetus leucocephalus), the National Bird of the USA.

    abstract：:The bald eagle (Haliaeetus leucocephalus) once experienced near-extinction but has since rebounded. For decades, bald eagles near the Wisconsin River, USA, have experienced a lethal syndrome with characteristic clinical and pathological features but unknown etiology. Here, we describe a novel hepacivirus-like virus (F...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-50580-8

    authors： Goldberg TL,Sibley SD,Pinkerton ME,Dunn CD,Long LJ,White LC,Strom SM

    更新日期：2019-10-18 00:00:00

  • Casimir forces exerted by epsilon-near-zero hyperbolic materials.

    abstract：:The Casimir force exerted on a gold dipolar nanoparticle by a finite-thickness slab of the natural hyperbolic material namely, the ortorhombic crystalline modification of boron nitride, is investigated. The main contribution to the force originates from the TM-polarized waves, for frequencies at which the parallel and...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73995-0

    authors： Nefedov IS,Rubi JM

    更新日期：2020-10-08 00:00:00

  • The role of industrial nitrogen in the global nitrogen biogeochemical cycle.

    abstract：:Haber-Bosch nitrogen (N) has been increasingly used in industrial products, e.g., nylon, besides fertilizer. Massive numbers of species of industrial reactive N (Nr) have emerged and produced definite consequences but receive little notice. Based on a comprehensive inventory, we show that (1) the industrial N flux has...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02579

    authors： Gu B,Chang J,Min Y,Ge Y,Zhu Q,Galloway JN,Peng C

    更新日期：2013-01-01 00:00:00

  • Scaffold-Hopping from Synthetic Drugs by Holistic Molecular Representation.

    abstract：:The discovery of novel ligand chemotypes allows to explore uncharted regions in chemical space, thereby potentially improving synthetic accessibility, potency, and the drug-likeness of molecules. Here, we demonstrate the scaffold-hopping ability of the new Weighted Holistic Atom Localization and Entity Shape (WHALES) ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-34677-0

    authors： Grisoni F,Merk D,Byrne R,Schneider G

    更新日期：2018-11-07 00:00:00

  • Immune cell type, cell activation, and single cell heterogeneity revealed by label-free optical methods.

    abstract：:Measurement techniques that allow the global analysis of cellular responses while retaining single-cell sensitivity are increasingly needed in order to understand complex and dynamic biological processes. In this context, compromises between sensitivity, degree of multiplexing, throughput, and invasiveness are often u...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-53428-3

    authors： Pavillon N,Smith NI

    更新日期：2019-11-19 00:00:00

  • Increased pollinator service and reduced pollen limitation in the fixed dune populations of a desert shrub.

    abstract：:Evaluations of restoration success usually focus on the structural aspects of ecosystems. Pollination, as an important functional aspect, is often overlooked. Here, the shifts in pollinator assemblage and pollen limitation in the desert shrub Caragana microphylla were examined along a restoration gradient in Horqin Sa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17253-w

    authors： Pan CC,Qu H,Feng Q,Liu LD,Zhao HL,Li YL,Li YQ,Zhang TH,Liu XP

    更新日期：2017-12-04 00:00:00