Abstract:
OBJECTIVE:To investigate whether bisphenol A (BPA) exposure is associated with uterine decidualization and embryo implantation failure in mice. DESIGN:Experimental animal study and in vitro study. SETTING:University-based infertility center. ANIMAL(S):ICR mice. INTERVENTION(S):Mice treated with different doses of BPA; Ishikawa cells cultured in medium of different concentrations of BPA. MAIN OUTCOME MEASURE(S):Embryo implantation sites, uterine weight, quantitative real-time reverse transcriptase-polymerase chain reaction, Western blot analysis, hematoxylin and eosin staining, and immunohistochemical, cell proliferation, and statistical analyses. RESULT(S):In the experiment of mouse model, administration of 1-100 μg/kg/day of BPA by gavage led to reduction of the number of embryo implantation sites in a dose-dependent manner; 100 μg/kg/day of BPA statistically significantly reduced the number of implantation sites compared with the control group. The uterine weight change (the wet weight of the decidualized uterine horn divided by the wet weight of the undecidualized uterine horn of the mouse) in groups exposed to BPA (100-10,000 μg/kg/day) were statistically significantly lower compared with the control group. Immunohistochemical analysis demonstrated that administration of 100, 1,000, or 10,000 μg/kg/day of BPA by gavage statistically significantly down-regulated the expression of epithelial Na+ channel α-subunit (ENaCα) in the luminal epithelial cells and desmin in decidual cells of the oil-induced decidualized uterine horns. Administration of 100 μg/kg/day BPA on embryo days 0.5-3.5 by gavage statistically significantly decreased the level of uterine serum and glucocorticoid-regulated kinase 1 (SGK1) protein expression on embryo days 4 and 6. After treatment with 0.001, 0.01, 0.1, or 1.0 μg/mL of BPA for 48 hours, the SGK1, ENaCα, and phospho-SGK1 protein expression of Ishikawa cells was down-regulated, and the effect of BPA on SGK1 could be abrogated by fulvestrant. CONCLUSION(S):Our study provides the first indication that BPA exposure at levels as low as 100 μg/kg/day can impair embryo implantation in mice and BPA can affect decidualization of the uterus in mouse model. Our results suggest that BPA can down-regulate SGK1 and ENaCα protein expression through estrogen receptors in Ishikawa cells.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Yuan M,Hu M,Lou Y,Wang Q,Mao L,Zhan Q,Jin Fdoi
10.1016/j.fertnstert.2017.12.003subject
Has Abstractpub_date
2018-04-01 00:00:00pages
735-744.e1issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(17)32119-2journal_volume
109pub_type
杂志文章abstract::Accurate localization of the source of androgen hypersecretion is critical to the appropriate surgical or medical management of women with virilization. Seven patients with virilization and hyperandrogenism of various causes were evaluated with the use of sequential studies: testosterone stimulation and suppression te...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract:OBJECTIVE:To determine whether a history of prior cesarean delivery (CD) makes ET more difficult and impacts pregnancy outcomes. DESIGN:Prospective cohort study. SETTING:Tertiary care military facility. PATIENT(S):One hundred ninety-four patients with previous delivery undergoing IVF/intracytoplasmic sperm injection...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.03.045
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVE:To describe a patient with uterine rupture in the subsequent pregnancy after hysteroscopic resection of a uterine septum. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 30-year-old nulliparous woman, with a history of a first trimester spontaneous abortion, hysteroscopic uterine septum resec...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.08.075
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of cryopreservation on the survival, cleavage, and morphology of embryos and on the implantation and embryonic loss rates of human zygotes obtained after ICSI compared with frozen-thawed zygotes obtained after traditional IVF. A further objective was to evaluate the same parameters in ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00021-1
更新日期:1998-04-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of paternal body mass index (BMI) on neonatal outcomes of singletons after frozen-thawed embryo transfer (FET) cycles. DESIGN:Retrospective cohort study. SETTING:Tertiary-care academic medical center. PATIENT(S):A total of 7,908 singleton newborns were divided into four categories...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2020.02.100
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To present a case of Marfan syndrome and concurrent congenital obstructive azoospermia. DESIGN:Case report. SETTING:Tertiary-care academic hospital. PATIENT(S):A man with Marfan syndrome and obstructive azoospermia. INTERVENTION(S):The patient was evaluated for azoospermia that was proved to be due to con...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)02868-0
更新日期:2001-12-01 00:00:00
abstract::The effectiveness of Buserelin (Hoechst-Roussel Pharmaceuticals, Inc., Somerville, NJ) (0.2 mg subcutaneously [SC] or 1.2 mg intranasally [IN] per day) and danazol (800 mg per day) in inducing ovarian suppression for the management of endometriosis was compared in a prospective randomized study. During 6 months of tre...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)60543-5
更新日期:1989-03-01 00:00:00
abstract::Ten infertile women 20 to 40 years of age, with a standard HSG diagnosis of unilateral proximal tubal obstruction, underwent a transvaginal catheterization and recanalization of the fallopian tubes. To set a fluoroscopic real-time guidance technique for improving the results of transvaginal catheterization and recanal...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55344-8
更新日期:1992-10-01 00:00:00
abstract:OBJECTIVE:To analyze the expression of estrogen (ER) and progesterone (PR) receptors in the smooth muscle component (SMC) of deep infiltrating endometriosis (DIE). DESIGN:A prospective clinical and pathologic study of 60 cases of DIE. SETTING:University Hospital Department of Gynacology. PATIENT(S):Sixty patients wi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.12.114
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:To describe the case of a Japanese woman with combined 17alpha-hydroxylase/17,20-lyase deficiency (congenital adrenal hyperplasia type V) and to discuss possible therapeutic procedures in such patients. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 26-year-old woman with secondary amenorrhe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00500-8
更新日期:2000-06-01 00:00:00
abstract:OBJECTIVE:To assess sexual function in women with spontaneous 46,XX primary ovarian insufficiency after at least 3 months of a standardized hormone replacement regimen. DESIGN:Cross-sectional cohort, controlled. SETTING:National Institutes of Health Clinical Research Center. PATIENT(S):Women with primary ovarian ins...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2007.08.040
更新日期:2008-11-01 00:00:00
abstract::This study was done to measure the bioactivity of luteinizing hormone (LH) in follicular fluid (FF) and to correlate this and other hormonal values with oocyte maturity in the spontaneous cycle. Twenty follicles (greater than 18 mm) from 18 ovulatory patients were obtained, and FF, according to oocyte maturity, was di...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49213-7
更新日期:1986-03-01 00:00:00
abstract:OBJECTIVE:Report of a rare case of consecutive spontaneous cervical pregnancies. DESIGN:Case study. SETTING:Teaching hospital. PATIENT(S):One healthy nulliparous woman in the early years of her fourth decade. INTERVENTION(S):The first of two cervical pregnancies was treated with two doses of methotrexate and subseq...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2003.06.012
更新日期:2004-01-01 00:00:00
abstract::There has been increasing interest in combining the oral agents clomiphene citrate (CC) and letrozole with gonadotropins in IVF: for poor responders to reduce the amount of gonadotropins used, and in normal responders to reduce the incidence of ovarian hyperstimulation (OHSS). In normal responders, mild stimulation wi...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2017.08.017
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:To study the distribution and progression of terminal hair growth in pregnant women and to determine the feasibility of a simplified scoring system for assessing hirsutism. DESIGN:Prospective follow-up observational study. SETTING:Academic hospital. PATIENT(S):A total of 115 pregnant women (discovery cohor...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.10.036
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:To determine the ectopic pregnancy rate with fresh versus frozen-thawed embryo transfers, and factors associated with ectopic pregnancy in patients undergoing IVF-ET. DESIGN:Retrospective analysis. SETTING:Institutional IVF center. PATIENT(S):A total of 3,183 patients who received 3,340 blastocysts transfe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.11.023
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:To identify the risk factors for ectopic pregnancy (EP) in women using an intrauterine device (IUD). DESIGN:Case-control study. SETTING:Auvergne region (France). PATIENT(S):Women using an IUD and suffering EP (243 cases) or having an intrauterine pregnancy (140 controls). INTERVENTION(S):None. MAIN OUTCO...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)01605-8
更新日期:2000-11-01 00:00:00
abstract:OBJECTIVE:To provide an up-to-date review of studies that have examined the relative role of endometrial development in in vitro fertilization (IVF) and embryo transfer (ET) treatment in relation to the treatment outcome. DATA IDENTIFICATION:The most important published studies and personal communications related to t...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)55008-0
更新日期:1992-05-01 00:00:00
abstract::Fresh and aged (24 hours after ovulation) human oocytes and recently ovulated mouse oocytes may be activated by exposure to acidified Tyrode's solution. No activation of either type of human oocyte was observed after exposure to hyaluronidase or pronase, but significant numbers of fresh mouse oocytes were activated af...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53279-8
更新日期:1990-02-01 00:00:00
abstract::Immunocytochemical, biochemical, and histologic analysis of endometriotic lesions and endometria from rhesus macaques with endometriosis revealed several distinctions between ectopic and eutopic endometrium. In lesions, unlike endometrium, neither the mean percentages of estrogen receptor positive (ER+) cells nor the ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract:OBJECTIVE:To compare the expression of the prostaglandin (PG) E(2) transporter multidrug resistance-associated protein 4 (MRP4) in eutopic and ectopic endometrial tissue from endometriosis patients with that of control subjects and to examine whether MRP4 is regulated by the antiinflammatory lipid lipoxin A(4) (LXA(4))...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.01.146
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVE:To describe the molecular, cytogenetic, immunohistochemical, and endocrinologic characteristics of a young 46,XY female with persistent müllerian structures and germ cell tumors in dysgenetic gonads. DESIGN:Descriptive case study. SETTING:Mackay Memorial Hospital and National Yang-Ming University, Taipei, T...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(99)00169-7
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVE:To see whether testicular sperm extraction could be used to perform intracytoplasmic sperm injection (ICSI) for men with nonobstructive azoospermia caused by maturation arrest. DESIGN:Uncontrolled prospective trial of an attempt to find occasional elongated spermatids or spermatozoa in testes of azoospermic ...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(16)58396-4
更新日期:1996-07-01 00:00:00
abstract::In summary, the luteal phase defect is a deficiency of corpus luteum progesterone steroidogenesis, either in amount or duration, or both. The clinical manifestations include either primary infertility or repeated first trimester abortions. The diagnosis can only be made clinically on the basis of a well-timed endometr...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)41769-3
更新日期:1976-04-01 00:00:00
abstract::Two women with the polycystic ovary syndrome were treated with bromocriptine (15 to 20 mg/day) in combination with spironolactone (100 mg/day). In the first woman the combined therapy induced a marked reduction of hirsutism, with ensuing ovulation and pregnancy. In the second woman, who had hyperthecosis and had been ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45554-8
更新日期:1981-06-01 00:00:00
abstract:OBJECTIVE:To investigate methods of fertility preservation in younger women exposed to adjuvant chemotherapy for breast cancer. DESIGN:Systematic review of literature. SETTING:Academic Department of Medical Oncology. PATIENT(S):Premenopausal women exposed to adjuvant chemotherapy for breast cancer. MAIN OUTCOME MEA...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2009.02.055
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To determine the effect of sperm manipulation before intracytoplasmic sperm injection (ICSI) on fertilization rates. DESIGN:Three methods of sperm manipulation before direct ICSI were compared in two sibling oocyte trials. SETTING:In vitro fertilization unit within a teaching hospital. PATIENTS:Patients un...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57988-6
更新日期:1995-12-01 00:00:00
abstract:OBJECTIVE:To study the in vivo mechanisms of action of ulipristal acetate (UPA) on uterine myomas. DESIGN:Retrospective histologic and immunohistochemical (IHC) study of myomas. SETTING:Academic research unit. PATIENT(S):Among 59 women with symptomatic myomas who underwent myomectomy, 42 were treated preoperatively ...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2015.04.025
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:The present study investigates the effect of prenatal hyperandrogenization on lipid metabolism and oxidant/antioxidant balance. DESIGN:Experimental study. SETTING:Research institute. ANIMAL(S):Pregnant Sprague Dawley rats were subcutaneously injected with 2 mg free T between days 16 and 19 of pregnancy, an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.10.017
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of tamoxifen treatment on sperm density, motility, vitality, and morphology. DESIGN:Placebo-controlled, randomized study. SETTING:Outpatient department. INTERVENTIONS:Patients were assigned to two comparable groups: group A (n = 122) was treated with 10 mg tamoxifen twice daily for ...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)56705-3
更新日期:1994-05-01 00:00:00