Abstract：
:Transient permeabilisation of the cell membrane is a critical step to introduce drugs or DNA into living cells, yet challenging for both biological research and therapeutic applications. To achieve this, electroporation (or electropermeabilisation) has become a widely used method due to its simplicity to deliver almost any biomolecule to any cell type. Although this method demonstrates promise in the field of drug/gene delivery, the underlying physical mechanisms of the response of the heterogeneous cell membrane to strong electric pulses is still unknown. In this study, we have investigated the role of gel-phase lipids in the electroporation of binary giant unilamellar vesicles (GUVs), composed from DPPC (gel-phase) and DPhPC (fluid-phase) lipids (molar ratio 8:2 and 2:8). We have observed that the exposure to electric pulses leads to expel of fluid-phase lipids and concomitant decrease in GUV size, whereas the gel-phase domains become buckled. Based on experiments on pure fluid-phase and gel-phase GUVs, we have found that fluid-phase lipids can be expelled by electrical forces and the highly viscous gel-phase lipids cannot. Moreover, our analyses suggest that pore formation occurs primarily in fluid-phase domains and that the pore size is similar in all GUVs containing fluid-phase lipids, irrespective of the gel-phase percentage.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Perrier DL,Rems L,Kreutzer MT,Boukany PEdoi
10.1038/s41598-018-23097-9subject
Has Abstractpub_date
2018-03-19 00:00:00pages
4758issue
1issn
2045-2322pii
10.1038/s41598-018-23097-9journal_volume
8pub_type
杂志文章abstract：:Misfolding and aggregation of prion protein (PrP) causes neurodegenerative diseases like Creutzfeldt-Jakob disease (CJD) and scrapie. Besides the consensus that spontaneous conversion of normal cellular PrPC into misfolded and aggregating PrPSc is the central event in prion disease, an alternative hypothesis suggests ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39213-2
更新日期：2019-02-21 00:00:00
abstract：:To explore a simple and easy-to-learn procedure for the isolation of human quiescent hepatic stellate cells (HSCs) that requires no advanced training. Thus reducing costs and increasing efficiency. This protocol will provide sufficient primary cells with minimal contaminants for future basic research on diseases assoc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49287-7
更新日期：2019-09-04 00:00:00
abstract：:Aldehyde dehydrogenase (ALDH) is a key enzyme for the catalytic oxidation of acetaldehyde to acetic acid. Genetic polymorphisms of ALDH2 have been associated with a wide range of diseases and cancers. However, little information is found about the association between ALDH2 polymorphisms and lumbar disc herniation (LDH...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31491-6
更新日期：2018-08-30 00:00:00
abstract：:The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61334-2
更新日期：2020-03-09 00:00:00
abstract：:In a myriad of engineering situations, we often hope to establish a model which can acquire load conditions around structures through flow features detection. A data-driven method is developed to predict the pressure on a cylinder from velocity distributions in its wake flow. The proposed deep learning neural network ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61450-z
更新日期：2020-03-10 00:00:00
abstract：:Rejuvenation is the configurational excitation of amorphous materials and is one of the more promising approaches for improving the deformability of amorphous metals that usually exhibit macroscopic brittle fracture modes. Here, we propose a method to control the level of rejuvenation through systematic thermal proces...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10545
更新日期：2015-05-26 00:00:00
abstract：:We propose a scheme for generating an entangled state for two atoms trapped in two separate cavities coupled to each other. The scheme is based on the competition between the unitary dynamics induced by the classical fields and the collective decays induced by the dissipation of two non-local bosonic modes. In this sc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17933-7
更新日期：2017-12-15 00:00:00
abstract：:A major translational challenge in the fields of therapeutic angiogenesis and regenerative medicine is the need to create functional microvasculature. The purpose of this study was to assess whether a potentially autologous endothelial cell (EC) source derived from human induced pluripotent stem cells (iPSC-ECs) can f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20966-1
更新日期：2018-02-08 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-67918-2
更新日期：2020-07-09 00:00:00
abstract：:Two embedded sulfur atoms in a novel [2 + 2] Schiff-base macrocyclic dinuclear Zn(II) complex were found to be easily autoxidized to the sulfone units on air exposure, and the resultant sulfone-functionalized macrocyclic complex was obtained by the post-modification strategy exhibiting enhanced antimicrobial activitie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15898-1
更新日期：2017-11-21 00:00:00
abstract：:This study was aimed at elucidating the changes of mandible symmetry in a rabbit model with low estrogen levels induced by ovariectomy (OVX) combined with temporomandibular joint (TMJ) anterior disc displacement without reduction (ADDWoR). 32 growing rabbits were randomly allocated into 4 groups; OVX group, ADDWoR gro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34023-4
更新日期：2018-10-23 00:00:00
abstract：:Ubiquitylation is a protein modification implicated in several cellular processes. This process is reversible by the action of deubiquinating enzymes (DUBs). USP45 is a ubiquitin specific protease about which little is known, aside from roles in DNA damage repair and differentiation of the vertebrate retina. Here, by ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32685-8
更新日期：2018-09-26 00:00:00
abstract：:Tumor protein D52 (TPD52) is amplified and overexpressed in breast and prostate cancers which are frequently characterised by dysregulated lipid storage and metabolism. TPD52 expression increases lipid storage in mouse 3T3 fibroblasts, and co-distributes with the Golgi marker GM130 and lipid droplets (LDs). We examine...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46156-1
更新日期：2019-07-05 00:00:00
abstract：:Fine particulate matter 2.5 (PM2.5) induces free radicals and oxidative stress in animals, leading to a range of illnesses. In this study, Ganoderma Microsporum immunomodulatory (GMI) proteins were administered to alleviate PM2.5-induced inflammatory responses in mother rats, and PM2.5-induced inflammatory responses a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38810-5
更新日期：2019-05-02 00:00:00
abstract：:Carbon fiber-synthetic foam core sandwich composites are widely used for many structural applications due to their superior mechanical performance and low weight. Unfortunately these structures typically have very poor acoustic performance. There is increasingly growing demand in mitigating this noise issue in sandwic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00403
更新日期：2012-01-01 00:00:00
abstract：:Enhancing endogenous cannabinoid (eCB) signaling has been considered as a potential strategy for the treatment of stress-related conditions. Fatty acid amide hydrolase (FAAH) represents the primary degradation enzyme of the eCB anandamide (AEA), oleoylethanolamide (OEA) and palmitoylethanolamide (PEA). This study desc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20895-z
更新日期：2018-02-05 00:00:00
abstract：:The moist savanna zone covers 0.5 × 106 km2 in West Africa and is characterized by very low soil N levels limiting primary production, but the ecology of nitrifiers in these (agro)ecosystems is largely unknown. We compared the effects of six agricultural practices on nitrifier activity, abundance and diversity at nine...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10185-5
更新日期：2017-08-31 00:00:00
abstract：:We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49445-x
更新日期：2019-09-10 00:00:00
abstract：:Better risk prediction and new molecular targets are key priorities in type 2 diabetes (T2D) research. Little is known about the role of the urine metabolome in predicting the risk of T2D. We aimed to use non-targeted urine metabolomics to discover biomarkers and improve risk prediction for T2D. Urine samples from two...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72456-y
更新日期：2020-10-05 00:00:00
abstract：:Flavonoids, due to their pharmacological attributes, have recently become target molecules for metabolic engineering in commonly consumed food crops. Strategies including expression of single genes and gene pyramiding have provided only limited success, due principally to the highly branched and complex biosynthetic p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05018
更新日期：2014-05-21 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-53160-y
更新日期：2019-11-05 00:00:00
abstract：:Clinical observations suggest neuronal control of bone remodeling. Sensory nerve fibers innervating bone, bone marrow and periosteum signal via neurotransmitters including substance P (SP). In previous studies we observed impaired biomechanical and structural bone parameters in tachykinin (Tac) 1-deficient mice lackin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27432-y
更新日期：2018-06-15 00:00:00
abstract：:This study investigates the association between an index of mesozooplankton biomass, derived from the Continuous Plankton Recorder survey and satellite-derived productivity fronts in the North Atlantic. While chlorophyll-a content (CHL) is commonly described as a proxy for phytoplankton biomass, the size of productivi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41212-2
更新日期：2019-03-18 00:00:00
abstract：:Tandem solar cells are suited for space applications due to their high performance, but also have to be designed in such a way to minimize influence of degradation by the high energy particle flux in space. The analysis of the subcell performance is crucial to understand the device physics and achieve optimized design...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02141-0
更新日期：2017-05-16 00:00:00
abstract：:The precise clinicopathologic significance of myeloid differentiation primary response gene (MYD88) L265P mutation in diffuse large B-cell lymphomas (DLBCLs) remains elusive. To investigate the frequency and clinicopathologic significance of the MYD88 L265P mutation in DLBCLs, we conducted a meta-analysis of 40 publis...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-017-01998-5
更新日期：2017-05-11 00:00:00
abstract：:The bronchoscopy, though usually safe, is occasionally associated with complications, such as pneumonia. However, the use of prophylactic antibiotics is not recommended by the guidelines of the British Thoracic Society. Thus far there are few reports of the risk factors for post-bronchoscopy pneumonia; the purpose of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76998-z
更新日期：2020-11-17 00:00:00
abstract：:The G protein-coupled pituitary adenylate cyclase-activating polypeptide receptor (PAC1R) is a potential therapeutic target for endocrine, metabolic and stress-related disorders. However, many questions regarding the protein structure and dynamics of PAC1R remain largely unanswered. Using microsecond-long simulations,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05815-x
更新日期：2017-07-14 00:00:00
abstract：:We present a method by which the spectral intensity of an ultrafast laser pulse can be accumulated at selected frequencies by a controllable amount. Using a 4-f pulse shaper we modulate the phase of the frequency components of a femtosecond laser. By inducing femtosecond filamentation with the modulated pulse, we can ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43367
更新日期：2017-03-07 00:00:00
abstract：:In situ reduction of selenite to elemental selenium (Se(0)), by microorganisms in sediments and soils is an important process and greatly affects the environmental distribution and the biological effects of selenium. However, the mechanism behind such a biological process remains unrevealed yet. Here we use Shewanella...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03735
更新日期：2014-01-17 00:00:00
abstract：:Abnormalities in actin cytoskeleton have been linked to Friedreich's ataxia (FRDA), an inherited peripheral neuropathy characterised by an early loss of neurons in dorsal root ganglia (DRG) among other clinical symptoms. Despite all efforts to date, we still do not fully understand the molecular events that contribute...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62050-7
更新日期：2020-03-23 00:00:00
