Abstract:
:Unfolded protein response (UPR) and endoplasmic reticulum (ER)-phagy are essential for cell homeostasis. Quantum dots (QDs), which have been widely used for biomedical applications, can accumulate in the kidney tissues and may cause renal dysfunction. However, the molecular mechanism of QDs-induced nephrotoxicity is still obscure. The present study was aimed to elucidate the role and mechanism of UPR and ER-phagy in QDs-induced nephrotoxicity. Herein, human embyronic kidney (HEK) cells were exposed to 15, 30, 45, and 60 nM cadmium telluride (CdTe)-QDs for 12 and 24 h. And CdTe-QDs (30-60 nM) inhibited the HEK cell viability. The clathrin-dependent endocytosis was determined as the main pathway of CdTe-QDs cellular uptake. Within cells, CdTe-QDs disrupted ER ultrastructure and induced UPR and FAM134B-dependent ER-phagy. Blocking UPR with inhibitors or siRNA rescued the FAM134B-dependent ER-phagy, which was triggered by CdTe-QDs. Moreover, suppression of UPR or FAM134B-dependent ER-phagy restored the cell vability. In vivo, mice were intravenously injected with 8 and 16 nmol/kg body weight CdTe-QDs for 24 h. Kidney was shown as one of highest distributed organs of CdTe-QDs, resulting in renal dysfunction, as well as UPR and FAM134B-dependent ER-phagy in it. Thus, for the first time, we demonstrated that ER-phagy can be triggered by nanomaterials both in vitro and in vivo. In addition, blocking of UPR and ER-phagy showed protective effects against CdTe-QDs-induced toxicity in kideny cells. Notably, a secreted alkaline phosphatase reporter gene system has been developed as a sensitive and rapid method for evaluating the ER quality under the exposure of nanomaterials.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Jiang S,Lin Y,Yao H,Yang C,Zhang L,Luo B,Lei Z,Cao L,Lin N,Liu X,Lin Z,He Cdoi
10.1007/s00204-018-2169-0subject
Has Abstractpub_date
2018-04-01 00:00:00pages
1421-1434issue
4eissn
0340-5761issn
1432-0738pii
10.1007/s00204-018-2169-0journal_volume
92pub_type
杂志文章abstract::The effect of various hepatotoxicants on urinary taurine and urinary creatine has been studied in the rat. Several hepatotoxic agents, carbon tetrachloride, thioacetamide, galactosamine and allyl alcohol which all caused hepatic necrosis (sometimes accompanied by steatosis), resulted in a rise in urinary taurine and i...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01974343
更新日期:1993-01-01 00:00:00
abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most potent tumor promoter ever tested in rodents. Although it is known that most of the effects of TCDD are mediated by binding to the aryl hydrocarbon receptor (AhR), the mechanisms leading to tumor promotion still remain to be elucidated. Loss of contact inhibition ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-004-0601-0
更新日期:2005-01-01 00:00:00
abstract::The first withdrawal of certain polybrominated diphenyl ethers flame retardants from the US market occurred in 2004. Since then, use of brominated non-PBDE compounds such as bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (BEH-TEBP) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB) in commercial formulations has incr...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-015-1489-6
更新日期:2016-03-01 00:00:00
abstract::Aluminum phosphide (AlP), one of the most commonly used pesticides worldwide, has been the leading cause of self-poisoning mortalities among many Asian countries. The heart is the main organ affected in AlP poisoning. Melatonin has been previously shown to be beneficial in reversing toxic changes in the heart. The pre...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-017-1998-6
更新日期:2017-09-01 00:00:00
abstract::Metabolic activation of the heterocyclic amine 3-amino -1,4-dimethyl-5 H-pyrido[4,3-b]indole (Trp-P-1) and 7-ethoxyresorufin O-deethylase (EROD) activity were examined in the chorioallantoic membrane (CAM) of 15-day-old chicken and 18-day-old eider duck embryos. The embryos were pretreated with an Ah receptor agonist,...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040000128
更新日期:2000-08-01 00:00:00
abstract::Neurotoxic properties of acrylamide and seven related compounds in rats were studied with regard to the effects on rotarod performance, morphology of nerves and neurotubulin. Compounds used in the present study were acrylamide, N-hydroxymethylacrylamide, N-isopropylacrylamide, methacrylamide, N-methylacrylamide, croto...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01239204
更新日期:1983-11-01 00:00:00
abstract::Chondrotoxicity is a rare toxicological finding which is observed in dogs after administration of quinolone antibacterials. To study this effect chondrocytes from articular cartilage of dogs were isolated, and incubated with quinolone derivatives. The effects on cell viability, mitochondrial dehydrogenase, and proteog...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01977402
更新日期:1993-01-01 00:00:00
abstract::To cope with the rapid onset of the life-threatening cholinergic crisis after poisoning with organophosphorus compounds, atropine-oxime preparations should be available in autoinjectors allowing i.m. administration also in the absence of a physician. Such a scenario is conceivable in the battlefield, when nerve agents...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050276
更新日期:1996-01-01 00:00:00
abstract::To explain the mechanism of renal injury caused by liver ischemia-reperfusion, we investigated biochemical and morphological changes in the liver and kidney in rats. After reperfusion following 60 min of liver ischemia, numerous changes were found. The level of serum transaminases and lipid peroxide formation in the l...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01969922
更新日期:1993-01-01 00:00:00
abstract::Morphological changes in mitochondria are observed early in the course of acetaminophen (AA)-induced hepatotoxicity, and mitochondrial dysfunction has been observed both in vivo and in vitro following exposure to AA. This study examined the early effects of AA exposure in vivo on mitochondrial respiration and evaluate...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050043
更新日期:1994-01-01 00:00:00
abstract::2,2-Dichloro-1,1,1-trifluoroethane (HCFC-123) has been developed as a substitute for ozone-depleting chlorofluorocarbons (CFCs). It is a structural analogue of halothane and similarities in the metabolic pathways and liver toxicity of both compounds have been described. The present study was initiated after an acciden...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040100237
更新日期:2001-07-01 00:00:00
abstract::4, 4'-Methylenedianiline (MDA) is used extensively as a curing agent in the production of elastomers and is classified as reasonably anticipated to be a human carcinogen based on sufficient evidence in animal experiments. Human N-acetyltransferase 1 (NAT1) and 2 (NAT2) catalyze the N-acetylation of aromatic amines and...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-019-02516-4
更新日期:2019-08-01 00:00:00
abstract::Pregnant C3H mice were exposed to a teratogenic dose of cyclophosphamide (CP, 20 mg/kg body weight) on the 10th day after copulation. Embryos or fetuses were examined 2-9 days later for gross abnormalities, weighed, and assayed for cephalic acetylcholinesterase (EC 3.1.1.7, AChE) activity, and haemoglobin content. In ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00570147
更新日期:1988-01-01 00:00:00
abstract::The release and availability of the carcinogenic component of the soluble azo dye Direct Red 46 and the insoluble azo pigment Pigment Yellow 17 were analyzed in Wistar rats using hemoglobin adducts as a dosimeter. The levels of hemoglobin adducts were found to be very low. Intestinal cleavage and release of 3,3'-dichl...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050024
更新日期:1994-01-01 00:00:00
abstract::A series of experiments was set up to elucidate the effects of pollution on marine and estuarine fish health, since the European flounder (Platichthys flesus) has shown a relatively high prevalence of (pre)neoplastic liver lesions and lymphocystis virus disease in Dutch coastal and estuarine waters. The hypothesis of ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040100213
更新日期:2001-04-01 00:00:00
abstract::As Drosophila melanogaster occupies an important position within the test battery for mutagens and carcinogens, it is of interest to study the xenobiotics metabolism of this insect. Likewise, the genetic control of these important enzyme systems falls within this interest. Our attempt was to get new strains, which sho...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00316345
更新日期:1984-11-01 00:00:00
abstract::Treatment of tuberculosis (TB) has been a therapeutic challenge because of not only the naturally high resistance level of Mycobacterium tuberculosis to antibiotics but also the newly acquired mutations that confer further resistance. Currently standardized regimens require patients to daily ingest up to four drugs un...
journal_title:Archives of toxicology
pub_type: 杂志文章,评审
doi:10.1007/s00204-016-1727-6
更新日期:2016-07-01 00:00:00
abstract::Possible induction of chromosomal aberrations and/or sister chromatid exchanges by astemizole was studied in vitro on human lymphocytes. In vivo chromosomal damage was assessed by a micronucleus test on rats and a dominant lethal test on both male and female mice. All these tests yielded negative results for astemizol...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00373399
更新日期:1982-07-01 00:00:00
abstract::With the development of nanotechnology, myriad types of novel materials have been discovered at the nanoscale, among which the most interesting material is graphene. However, the toxicity data available on graphene are extremely limited. In this study, we explored toxic response of commercially available graphene nano...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-014-1303-x
更新日期:2015-09-01 00:00:00
abstract::The efficacy of oxime (HI-6, toxogonin or PAM Cl) therapy against GF (cyclohexyl methylphosphonofluoridate) poisoning was assessed in mice. It was found that the combinations of atropine and either toxogonin or HI-6 were effective therapies against GF poisoning. PAM therapy was ineffective. HI-6 was the only oxime whi...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF02342509
更新日期:1992-01-01 00:00:00
abstract::Sodium diethyldithiocarbamate (DETC) is the main metabolite of disulfiram. Recently, we reported that mechanism of disulfiram cytotoxicity in V79 cells might be partially connected with thiol redox-state imbalance. Here, we examined the effect of DETC on the level of intracellular glutathione (GSH), protein oxidation ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-012-0909-0
更新日期:2012-12-01 00:00:00
abstract::Ethylenediaminetetraacetic acid (EDTA) is widely used in food and other industries to sequester metal ions and to prevent their disadvantageous effects. The objective of the current study was to evaluate the protective effect of Panax ginseng extract standardized with ginsenoside Rg3 (ginsenoside Rg3 content was 3.6% ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-007-0237-y
更新日期:2008-03-01 00:00:00
abstract::Concerning molecular and cellular mechanisms of aluminum toxicity, recent studies support the hypothesis that interactions of aluminum ions with elements of signal transduction pathways are apparently primary events in cells. In the case of the phosphoinositide-associated signalling pathway of neuroblastoma cells, gua...
journal_title:Archives of toxicology
pub_type: 杂志文章,评审
doi:10.1007/s002040050023
更新日期:1994-01-01 00:00:00
abstract::The effect of lindane and heptachlor on haem metabolism was studied with the aim to elucidate the mechanism of their porphyrinogenic action. The effects of these compounds on delta-aminolaevulinate synthase (ALA-S) and ferrochelatase were evaluated and the mechanism of increase of ALA-S activity was especially studied...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050566
更新日期:1998-11-01 00:00:00
abstract::The aim of our study was to determine the period of maximum sensitivity for the induction of characteristic malformations with thalidomide (TH) in Himalayan rabbits. TH was administered orally in different doses (50, 100, 150 and 200 mg/kg) four times at 24-h intervals starting at 192 h of gestation. The malformations...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00295758
更新日期:1987-07-01 00:00:00
abstract::The responsiveness of the hepatic supernatant NAD+-dependent aldehyde dehydrogenase with a high Km value (high Km-AldDH) to phenobarbital (PB) and 3-methylcholanthrene (3-MC) treatment was studied in male rats of three strains; Wistar, Long-Evans, and Sprague-Dawley. A remarkable strain difference in the response of t...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00333620
更新日期:1979-12-01 00:00:00
abstract::Inhabitants of a private home suffered from symptoms possibly due to dichloroacetylene intoxication. Subsequent anamnesis revealed that abundant amounts of trichloroethylene had been used to remove a wax coating from a concrete-lined stone floor. This prompted us to examine whether dichloroacetylene could have been fo...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00349074
更新日期:1984-12-01 00:00:00
abstract::The pharmacokinetics of styrene were investigated in male Sprague-Dawley rats and male B6C3F1 mice using the closed chamber technique. Animals were exposed to styrene vapors of initial concentrations ranging from 550 to 5000 ppm, or received intraperitoneal (i.p.) doses of styrene from 20 to 340 mg/kg or oral (p.o.) d...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01969264
更新日期:1993-01-01 00:00:00
abstract::Chronic ciprofibrate administration resulted in distinct differences in hepatic responses between the two species examined. In the rat, hepatomegaly was observed with the coordinate induction of carnitine acetyltransferase, peroxisomal beta-oxidation and cytochrome P450IVA1 activities. The latter induction of cytochro...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF02034935
更新日期:1991-01-01 00:00:00
abstract::The effect of intracerebroventricular (i.c.v.) administration of cadmium or magnesium on central calcium-dependent blood pressure regulation was investigated. The systolic blood pressure of spontaneously hypertensive rats (SHR; male, 13 weeks of age) decreased following i.c.v. administration of cadmium chloride (20 nm...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050644
更新日期:2000-03-01 00:00:00