Development of a new biochip array for APOE4 classification from plasma samples using immunoassay-based methods.

abstract：:Venous blood sampling (phlebotomy) is the most common invasive procedure performed in patient care. Guidelines on the correct practice of phlebotomy are available, including the H3-A6 guideline issued by the Clinical Laboratory Standards Institute (CLSI). As the quality of practices and procedures related to venous bl...

journal_title：Clinical chemistry and laboratory medicine

authors： van Dongen-Lases EC,Cornes MP,Grankvist K,Ibarz M,Kristensen GB,Lippi G,Nybo M,Simundic AM,Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM).

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:Established general risk score models for intensive care patients incorporate several clinical and laboratory data. However, the collection, documentation and classification of clinical data are time-consuming, incur labor-related costs, and are dependent on the experience of the examiner. Therefore, in the ...

journal_title：Clinical chemistry and laboratory medicine

authors： Stachon A,Segbers E,Hering S,Kempf R,Holland-Letz T,Krieg M

更新日期：2008-01-01 00:00:00

abstract：:The annual inter- and intra-individual biological variation, including the circannual rhythmic variation, of the serum concentrations of magnesium and ionized calcium has been investigated in a group of 51 apparently healthy volunteers. Venous blood specimens were collected on intervals of once a month within a one-ye...

journal_title：Clinical chemistry and laboratory medicine

authors： Valero-Politi J,Ginard-Salvá M,González-Alba JM

更新日期：2001-01-01 00:00:00

abstract：:Classical genetic studies involving the analysis of pedigrees and recurrence risk within families have defined the extent to which genetic factors contribute to the aetiology of multiple sclerosis. Limited progress has been made in identifying the number and topography of genetic loci contributing to susceptibility th...

journal_title：Clinical chemistry and laboratory medicine

authors： Compston A

更新日期：2000-02-01 00:00:00

abstract：:The commutability of 13 control materials was evaluated by performing parallel measurements on two different analysers: a Synchron CX-5 Delta from Beckman-Coulter and a Vitros 950 from Ortho-Clinical Diagnostics. Twenty three clinical chemistry analytes (substrates, electrolytes and enzymatic activities) were determin...

journal_title：Clinical chemistry and laboratory medicine

authors： Brion E,Lessinger JM,Gould N,Leyendecker J,Férard G

更新日期：2002-06-01 00:00:00

abstract：BACKGROUND:The discovery of mutations in the epidermal growth factor receptor gene (EGFR) related to the clinical response to tyrosine kinase inhibitors, has transformed the management of non-small cell lung cancer (NSCLC). Several methods have been developed for determination of mutations in EGFR, with different sensi...

journal_title：Clinical chemistry and laboratory medicine

authors： Martínez-Carretero C,Pascual FI,Rus A,Bernardo I

更新日期：2017-10-26 00:00:00

abstract：BACKGROUND:Despite remarkable advances in technology and laboratory automation, results of laboratory testing still suffer from a high degree of preanalytical variability. Although there is no definitive evidence, the use of small-gauge needles for venipuncture is usually discouraged to reduce the chance of producing u...

journal_title：Clinical chemistry and laboratory medicine

authors： Lippi G,Salvagno GL,Montagnana M,Brocco G,Cesare Guidi G

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:The biochemical determination of cardiac natriuretic peptides, primarily brain natriuretic peptide (BNP) and the amino-terminal fragment of its pro-hormone proBNP (NT-proBNP), are reliable tools for diagnosing cardiac disease, establishing prognosis and evaluating the effectiveness of treatment. These biomar...

journal_title：Clinical chemistry and laboratory medicine

authors： Zaninotto M,Mion MM,Di Serio F,Caputo M,Ottomano C,Plebani M

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND:Anti-cyclic citrullinated peptide (CCP) antibody is emerging as an important diagnostic marker for rheumatoid arthritis (RA). We evaluated the analytical and diagnostic performance of the ARCHITECT anti-CCP (Abbott Diagnostics), a new fully automated chemiluminescent microparticle immunoassay. METHODS:Serum...

journal_title：Clinical chemistry and laboratory medicine

authors： Hwang SM,Kim JO,Yoo YM,Shin S,Kim JQ,Park MH,Song EY

更新日期：2010-02-01 00:00:00

abstract：:Background uPA and PAI-1 are breast cancer biomarkers that evaluate the benefit of chemotherapy (CT) for HER2-negative, estrogen receptor-positive, low or intermediate grade patients. Our objectives were to observe clinical routine use of uPA/PAI-1 and to build a new therapeutic decision tree integrating uPA/PAI-1. Me...

journal_title：Clinical chemistry and laboratory medicine

authors： Reix N,Lodi M,Jankowski S,Molière S,Luporsi E,Leblanc S,Scheer L,Ibnouhsein I,Benabu JC,Gabriele V,Guggiola A,Lessinger JM,Chenard MP,Alpy F,Bellocq JP,Neuberger K,Tomasetto C,Mathelin C

更新日期：2019-05-27 00:00:00

abstract：:Carbohydrate-deficient transferrin (CDT) is a descriptive term used for a temporary change in the transferrin glycosylation profile caused by alcohol, and used as a biomarker of chronic high alcohol consumption. The use of an array of methods for measurement of CDT in various absolute or relative amounts, and sometime...

journal_title：Clinical chemistry and laboratory medicine

authors： Helander A,Wielders JP,Jeppsson JO,Weykamp C,Siebelder C,Anton RF,Schellenberg F,Whitfield JB,IFCC Working Group on Standardization of Carbohydrate-Deficient Transferrin (WG-CDT).

更新日期：2010-11-01 00:00:00

abstract：:The malondialdehyde-thiobarbituric acid assay is widely used to study lipid peroxidation. Among the various methods used to perform the assay, the most widely accepted is the quantification of malondialdehyde using the thiobarbituric acid reaction, followed by reversed-phase chromatography. However, unacceptable resul...

journal_title：Clinical chemistry and laboratory medicine

authors： Gonzalo R,Vives-Bauza C,Andreu AL,García-Arumí E

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:The usefulness of serum antibodies to common food antigens (immunoglobulin G4; IgG4) assay in management of patients suffering from food intolerance was assessed. METHODS:A total of 22 asymptomatic healthy subjects and 68 patients with symptoms referred for suspected food intolerance were studied. Serum IgG...

journal_title：Clinical chemistry and laboratory medicine

authors： Bernardi D,Borghesan F,Faggian D,Bianchi FC,Favero E,Billeri L,Plebani M

更新日期：2008-01-01 00:00:00

abstract：:Volatile biomarker analysis in exhaled breath is becoming one of the desirable strategies for cancer detection because it may offer a relatively inexpensive, rapid, and non-invasive screening method for early diagnosis. Breath analysis has attracted a considerable amount of scientific and clinical interest over the pa...

journal_title：Clinical chemistry and laboratory medicine

authors： Badjagbo K

更新日期：2012-11-01 00:00:00

abstract：:Betaine serves as a methyl donor in a reaction converting homocysteine to methionine, catalysed by the enzyme betaine-homocysteine methyltransferase. It has been used for years to lower the concentration of plasma total homocysteine (tHcy) in patients with homocystinuria, and has recently been shown to reduce fasting ...

journal_title：Clinical chemistry and laboratory medicine

authors： Ueland PM,Holm PI,Hustad S

更新日期：2005-01-01 00:00:00

abstract：:There is some evidence from recent observational studies that hyperhomocysteinemia is a risk factor for cognitive dysfunction, including Alzheimer's disease and vascular dementia. There are only a few intervention studies, and the results are disappointing for such a frequent disease. Prospective double-blind and plac...

journal_title：Clinical chemistry and laboratory medicine

authors： Joosten E

更新日期：2001-08-01 00:00:00

abstract：BACKGROUND:Post-menopausal osteoporosis (PO) affecting a large fraction of elderly women, is triggered by the decline in 17β-estradiol (E2) level. Experimental studies in animal models and cell cultures have suggested that the fall in E2 might contribute to developing oxidative stress (OS) which in turn is believed to ...

journal_title：Clinical chemistry and laboratory medicine

authors： Cervellati C,Bonaccorsi G,Cremonini E,Bergamini CM,Patella A,Castaldini C,Ferrazzini S,Capatti A,Picarelli V,Pansini FS,Massari L

更新日期：2013-02-01 00:00:00

abstract：:Over the past decades, the randomized controlled trial has entered an era of continuous improvement and has gradually become accepted as the most effective way of determining the relative efficacy and toxicity of new therapies because it controls for placebo and time effects. However, even sensitive and properly desig...

journal_title：Clinical chemistry and laboratory medicine

authors： Cleophas TJ

更新日期：1999-11-01 00:00:00

abstract：:The availability of so-called high-sensitivity troponin assays (hsTn) has scored a compelling goal for laboratory medicine, allowing the safe clinical application of international recommendations for the definition of acute myocardial infarction (AMI). However, the introduction of hsTn has not been welcomed by clinici...

journal_title：Clinical chemistry and laboratory medicine

authors： Ferraro S,Panteghini M

更新日期：2015-04-01 00:00:00

abstract：:Elevated plasma levels of angiotensin converting enzyme (ACE) are associated with granulomatous diseases. However, several families of autosomal dominant hyper-ACE-emia without disease association have already been reported. Recently, the ACE mutation c.3705C>T (Pro1199Leu) was identified as the genetic correlate in E...

journal_title：Clinical chemistry and laboratory medicine

authors： Semmler A,Stein RW,Caplan L,Danilov SM,Klockgether T,Linnebank M

更新日期：2006-01-01 00:00:00

abstract：:We estimated the frequencies of phenotype (isoelectric focusing; IEF) vs. genotype (PCR/Hhal) discordance in a sample of an aged population (> 65 years). Both phenotype and genotype techniques have been used in the study of apolipoprotein E (apoE) polymorphism in 125 elderly subjects. The discordance between phenotype...

journal_title：Clinical chemistry and laboratory medicine

authors： Dupuy AM,Badiou S,Ritchie K,Mas E,Descomps B,Cristol JP,Touchon J

更新日期：2001-05-01 00:00:00

abstract：:Primary biliary cholangitis (PBC), also known as primary biliary cirrhosis, is an autoimmune disease of the liver characterized by anti-mitochondrial antibodies (AMA) in 90%-95% of patients. The aim of this study was to evaluate the diagnostic value of several serum biomarkers in patients with PBC but negative for AMA...

journal_title：Clinical chemistry and laboratory medicine

authors： de Liso F,Matinato C,Ronchi M,Maiavacca R

更新日期：2017-11-27 00:00:00

abstract：BACKGROUND:Query fever (Q fever) is a zoonotic infection, caused by the intracellular Gram-negative coccobacillus Coxiella burnetii. From 2007 until 2010, a large Q fever outbreak has occurred in the Netherlands. We studied traditional and less common inflammation markers in seronegative and seropositive patients with ...

journal_title：Clinical chemistry and laboratory medicine

authors： Vissers E,de Jager CP,Hilbink M,Kusters R,van Gageldonk-Lafeber AB,Wever PC

更新日期：2012-02-04 00:00:00

abstract：:In our efforts to develop diagnostic tests for complex multifactorial disorders, and to assist the research community in evaluating genetic markers for predisposition to cardiovascular disease, we have developed a prototype assay to genotype up to 35 variable sites among 15 genes. The candidate markers in this panel w...

journal_title：Clinical chemistry and laboratory medicine

authors： Cheng S,Pallaud C,Grow MA,Scharf SJ,Erlich HA,Klitz W,Pullinger CR,Malloy MJ,Kane JP,Siest G,Visvikis S

更新日期：1998-08-01 00:00:00

abstract：:Glutathione S-transferase (GST) isoenzymes are involved in the detoxification of major carcinogens present in tobacco smoke. It is thus conceivable that deficiency in GST activity due to homozygous deletions of the GSTM1 and GSTT1 genes (the null genotypes) may modulate susceptibility to smoking-induced cancers. The i...

journal_title：Clinical chemistry and laboratory medicine

authors： Hong YJ,Lee JK,Lee GH,Hong SI

更新日期：2000-09-01 00:00:00

abstract：BACKGROUND:Low back pain (LBP) is a very frequent condition, affecting most people at some point throughout their life. This cross-sectional study was aimed to investigate a selected panel of cytokines and inflammatory biomarkers in patients with or without LBP. METHODS:The study population consisted of 104 patients d...

journal_title：Clinical chemistry and laboratory medicine

authors： Lippi G,Dagostino C,Buonocore R,Aloe R,Bonaguri C,Fanelli G,Allegri M

更新日期：2017-08-28 00:00:00

abstract：:Background Patients with cystic fibrosis (CF) have a reduced intestinal absorption of cholesterol and in a preliminary study we observed differences in plasma sterol profile between patients with pancreatic sufficiency (PS) and those with pancreatic insufficiency (PI). Therefore, we hypothesized that the sterol analys...

journal_title：Clinical chemistry and laboratory medicine

authors： Gelzo M,Iacotucci P,Sica C,Liguori R,Comegna M,Carnovale V,Dello Russo A,Corso G,Castaldo G

更新日期：2020-09-25 00:00:00

abstract：BACKGROUND:The use of laboratory tests has been increasing in recent years due to various factors affecting laboratories, physicians, legal aspects, or patients themselves. METHODS:The efficacy of laboratory tests must be evaluated on the basis of the clinical benefits that they provide in terms of prevention, diagnos...

journal_title：Clinical chemistry and laboratory medicine

authors： Alonso-Cerezo MC,Martín JS,García Montes MA,de la Iglesia VM

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Inflammation plays an imperative role in the etiology of cardiovascular diseases (CVD). The role of cytokines in the development and progression of idiopathic dilated cardiomyopathy (IDCM) is still uncertain. The current study was conducted to evaluate the association of tumor necrosis factor-α (TNF-α) -238G...

journal_title：Clinical chemistry and laboratory medicine

authors： Liaquat A,Shauket U,Ahmad W,Javed Q

更新日期：2015-02-01 00:00:00

abstract：BACKGROUND:Serum immunoglobulin free light chains (FLC) are secreted into circulation by plasma cells as a by-product of immunoglobulin production. In a healthy individual the population of FLC is polyclonal as no single cell is secreting more FLC than the total immunoglobulin secreting cell population. In a person wit...

journal_title：Clinical chemistry and laboratory medicine

authors： Barnidge DR,Dispenzieri A,Merlini G,Katzmann JA,Murray DL

更新日期：2016-06-01 00:00:00