Exhaled breath analysis for early cancer detection: principle and progress in direct mass spectrometry techniques.

abstract：BACKGROUND:Query fever (Q fever) is a zoonotic infection, caused by the intracellular Gram-negative coccobacillus Coxiella burnetii. From 2007 until 2010, a large Q fever outbreak has occurred in the Netherlands. We studied traditional and less common inflammation markers in seronegative and seropositive patients with ...

journal_title：Clinical chemistry and laboratory medicine

authors： Vissers E,de Jager CP,Hilbink M,Kusters R,van Gageldonk-Lafeber AB,Wever PC

更新日期：2012-02-04 00:00:00

abstract：:The methods for detecting and measuring autoantibodies have evolved markedly in recent years, encompassing three generations of analytical technologies. Many different immunoassay methods have been developed and used for research and laboratory practice purposes, from the early conventional (or monoplex) analytical me...

journal_title：Clinical chemistry and laboratory medicine

authors： Tozzoli R,Bonaguri C,Melegari A,Antico A,Bassetti D,Bizzaro N

更新日期：2013-01-01 00:00:00

abstract：:Folate measurements, particularly for whole blood, show wide inter-laboratory and inter-methodology variability. This variability appears to be due in part to the lack of internationally accepted reference materials. A whole blood haemolysate, lyophilised in ampoules and designated 95/528, was therefore evaluated by 1...

journal_title：Clinical chemistry and laboratory medicine

authors： Thorpe SJ,Sands D,Heath AB,Hamilton MS,Blackmore S,Barrowcliffe T

更新日期：2004-05-01 00:00:00

abstract：:The discovery and development of new biomarkers continues to be an exciting and promising field. Improvement in prediction of risk of developing disease is one of the key motivations in these pursuits. Appropriate statistical measures are necessary for drawing meaningful conclusions about the clinical usefulness of th...

journal_title：Clinical chemistry and laboratory medicine

authors： Pencina MJ,D'Agostino RB,Vasan RS

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Cigarette smoking, a cardiovascular risk factor leading to oxygen free radical formation, is involved in the development of serious pathological conditions. On the other hand, a healthy diet and adequate supplementation can help prevent many diseases. The aim of our study was to evaluate in healthy light smo...

journal_title：Clinical chemistry and laboratory medicine

authors： Bamonti F,Novembrino C,Ippolito S,Soresi E,Ciani A,Lonati S,Scurati-Manzoni E,Cighetti G

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Sequencing based mutation screening assays of genes encompassing large numbers of exons could be substantially optimized by multiplex PCR, which enables simultaneous amplification of many targets in one reaction. In the present study, a multiplex PCR protocol originally developed for fragment analysis was ev...

journal_title：Clinical chemistry and laboratory medicine

authors： Horn MP,Mäder-Heinemann G,Andrey G,Largiadèr CR

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Polymorphisms of the gene for C-reactive protein (CRP) alter baseline serum CRP concentrations. The impact of polymorphisms of the CRP gene (genotype) on the normal range for CRP concentrations (phenotype) has not been determined. This study evaluated the median serum CRP concentrations in normal subjects st...

journal_title：Clinical chemistry and laboratory medicine

authors： Jones J,Chen LS,Baudhuin L,Peterson S,Harmsen WS,Zinsmeister AR,McConnell J,Sandborn WJ

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Vitamin B12 deficiency and bone fractures are common in vegetarians. However, a direct relationship between vitamin B12 status and bone metabolism in vegetarians has not been tested sufficiently. METHODS:Our study included 96 vegetarians (23 German vegans, and 54 German and 19 Indian lacto-, lacto-ovo-veget...

journal_title：Clinical chemistry and laboratory medicine

authors： Herrmann W,Obeid R,Schorr H,Hübner U,Geisel J,Sand-Hill M,Ali N,Herrmann M

更新日期：2009-01-01 00:00:00

abstract：:Automation is considered one of the most important breakthroughs in the recent history of laboratory diagnostics. In a model of total laboratory automation (TLA), many analyzers performing different types of tests on different sample matrices are physically integrated as modular systems or physically connected by asse...

journal_title：Clinical chemistry and laboratory medicine

authors： Lippi G,Da Rin G

更新日期：2019-05-27 00:00:00

abstract：BACKGROUND:Antinuclear autoantibody determination relies on an initial screening step using immunofluorescence on HEp2 cells. This may be followed by anti-deoxyribonucleic acid (DNA) determination, if the fluorescence of nuclei is homogeneous. We assessed the validity of a restricted algorithm and compared this to a mo...

journal_title：Clinical chemistry and laboratory medicine

authors： Servais G,Karmali R,Guillaume MP,Badot V,Duchateau J,Corazza F

更新日期：2009-01-01 00:00:00

abstract：:Age-related macular degeneration (AMD) is a sight threatening eye disease that affects millions of humans over the age of 65 years. It is considered to be the major cause of irreversible blindness in the elderly population in the developed world. The disease is prevalent in Europe and the United States, which has a la...

journal_title：Clinical chemistry and laboratory medicine

authors： Kokotas H,Grigoriadou M,Petersen MB

更新日期：2011-04-01 00:00:00

abstract：:Objectives Cerebrospinal fluid (CSF) is a clear, colorless body fluid filling the central nervous system. The determination of the CSF total protein (TP) content represents an important screening test of various pathologies. We aimed to address the effect of sex and age on CSF-TP content and the use of the current upp...

journal_title：Clinical chemistry and laboratory medicine

authors： Castellazzi M,Pizzicotti S,Lombardo I,Alfiero S,Morotti A,Pellegatti P,Negri G,Natali L,Ferri C,Fainardi E,Bellini T,Pugliatti M

更新日期：2020-10-25 00:00:00

abstract：:Laboratory Medicine organizations and their professional members have a goal and responsibility to benefit the health and wellbeing of the patients and communities they serve. Newer genetics and biochemical techniques raise significant issues of community concern, impacting on privacy, informed consent, access to and ...

journal_title：Clinical chemistry and laboratory medicine

authors： Burnett L,McQueen MJ,Jonsson JJ,Torricelli F,IFCC Taskforce on Ethics 13.7.

更新日期：2007-01-01 00:00:00

abstract：:Diabesity, that is to say, obesity-dependent diabetes, has emerged as a major public health problem. Though diabesity is basically explained by insulin resistance and pancreatic beta cell dysfunction, new paradigms have evolved to explain these alterations in the context of the modern epidemics of obesity and diabetes...

journal_title：Clinical chemistry and laboratory medicine

authors： Schmidt MI,Duncan BB

更新日期：2003-09-01 00:00:00

abstract：:Overcrowding of the emergency department (ED) is rapidly becoming a global challenge and a major source of concern for emergency physicians. The evaluation of cardiac biomarkers is critical for confirming diagnoses and expediting treatment decisions to reduce overcrowding, however, physicians currently face the dilemm...

journal_title：Clinical chemistry and laboratory medicine

authors： Di Somma S,Zampini G,Vetrone F,Soto-Ruiz KM,Magrini L,Cardelli P,Ronco C,Maisel A,Peacock FW

更新日期：2014-10-01 00:00:00

abstract：BACKGROUND:The pathogenesis of pre-eclampsia (PE) is incompletely understood. The placenta is considered to play a key role in this disease. Recent research showed that many microRNAs (miRNAs) are expressed in human placenta. Our aim in this study was to determine differential expression of miRNAs in placenta with seve...

journal_title：Clinical chemistry and laboratory medicine

authors： Hu Y,Li P,Hao S,Liu L,Zhao J,Hou Y

更新日期：2009-01-01 00:00:00

abstract：:Background Intravascular hemolysis and in vitro hemolysis are prevalent contributors to failed blood sample analysis in the routine hospital laboratory. Interferences by hemoglobin in spectrophotometric and certain enzyme activity assays is the major causative factor. Methods By exploiting the hemoglobin-binding prope...

journal_title：Clinical chemistry and laboratory medicine

authors： Sæderup KL,Revsholm J,Richardt PB,Christiansen SH,Hennig D,Moestrup SK,Graversen JH

更新日期：2019-10-25 00:00:00

abstract：:Removal of low molecular weight proteins from plasma by kidneys depends on glomerular filtration rate (GFR), protein-glomerular membrane electric charge, steric interactions and a number of functionally active nephrons present in the kidneys. There is a well documented relationship between the concentration of low mol...

journal_title：Clinical chemistry and laboratory medicine

authors： Naskalski J,Anyszek T,Surdacka E,Hoyer B,Sulowicz W

更新日期：2004-03-01 00:00:00

abstract：:Glycosylation is among the most important post-translational modifications for proteins and is of intrinsic complex character compared with DNAs and naked proteins. Indeed, over 50%-70% of proteins in circulation are glycosylated, and the "sweet attachments" have versatile structural and functional implications. Both ...

journal_title：Clinical chemistry and laboratory medicine

authors： Wang M,Zhu J,Lubman DM,Gao C

更新日期：2019-03-26 00:00:00

abstract：:Background For pregnant women with suspected preeclampsia, the soluble fms-like tyrosine-kinase 1 (sFlt-1)/placental growth factor (PlGF) ratio is a biomarker to aid diagnosis. We performed method comparisons between Elecsys® and Kryptor sFlt-1 and PlGF immunoassays and assessed the diagnostic performance for preeclam...

journal_title：Clinical chemistry and laboratory medicine

authors： Stepan H,Hund M,Dilba P,Sillman J,Schlembach D

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:An E670G polymorphism of the exon 12 of the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene was recently found to be associated with increased plasma low-density lipoprotein cholesterol (LDL-C) levels and severity of coronary atherosclerosis. This case-control study tested for a possible link betw...

journal_title：Clinical chemistry and laboratory medicine

authors： Hsu LA,Teng MS,Ko YL,Chang CJ,Wu S,Wang CL,Hu CF

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:CD4+CD25+ forkhead box P3 (FOXP3)+ regulatory T cells (Tregs) accumulate in malignant tumors and negatively regulate antitumor immunity. However, the clinical significance of Tregs in HCC remains unclear. To determine the prognostic value of Tregs, we conducted a retrospective study on 264 patients with Barc...

journal_title：Clinical chemistry and laboratory medicine

authors： Li F,Guo Z,Lizée G,Yu H,Wang H,Si T

更新日期：2014-09-01 00:00:00

abstract：:Background The aim of this study was to evaluate the diagnostic performance of cerebrospinal fluid (CSF) free light chains (FLCs) in the diagnosis of Lyme neuroborreliosis (LNB). Methods Serum and CSF levels of κ- and λ-FLC, albumin and total concentration of immunoglobulin M (IgM) were determined together with CSF ch...

journal_title：Clinical chemistry and laboratory medicine

authors： Tjernberg I,Johansson M,Henningsson AJ

更新日期：2019-11-26 00:00:00

abstract：:The early diagnosis of rheumatoid arthritis (RA) has become a priority owing to the availability of effective disease-modifying agents that can improve patient wellbeing and influence the clinical outcome. However, this represents a real challenge, as no clinical, radiological or immunological features are pathognomon...

journal_title：Clinical chemistry and laboratory medicine

authors： Bizzaro N

更新日期：2007-01-01 00:00:00

abstract：:There is currently no well-established biomarker for Parkinson's disease. The need to better diagnose the condition, define the subtypes of disease, and follow its course independent of any symptomatic drug effects is well-established. In this review, we will begin by reviewing the evidence for biological fluid biomar...

journal_title：Clinical chemistry and laboratory medicine

authors： Breen DP,Michell AW,Barker RA

更新日期：2011-03-01 00:00:00

abstract：:We developed a time-resolved-fluoro-immunoassay to measure cortisol binding globulin (CBG) in serum. It is a microtitre plate, solid phase, reagent excess, sandwich assay in which the same polyclonal antiserum is used as a source of capture and labeled antibodies. The results of this assay were shown to be reliable an...

journal_title：Clinical chemistry and laboratory medicine

authors： Bonte HA,van den Hoven RJ,van der Sluijs Veer G,Vermes I

更新日期：1999-02-01 00:00:00

abstract：BACKGROUND:After the re-introduction of ImmunoCAP® ISAC sIgE 112 on the market, we undertook a study to evaluate the performance of this multiplex-based immunoassay for IgE measurements to allergen components. METHODS:The study was carried out at 22 European and one South African site. Microarrays from different batch...

journal_title：Clinical chemistry and laboratory medicine

authors： van Hage M,Schmid-Grendelmeier P,Skevaki C,Plebani M,Canonica W,Kleine-Tebbe J,Nystrand M,Jafari-Mamaghani M,Jakob T

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Inflammation plays an imperative role in the etiology of cardiovascular diseases (CVD). The role of cytokines in the development and progression of idiopathic dilated cardiomyopathy (IDCM) is still uncertain. The current study was conducted to evaluate the association of tumor necrosis factor-α (TNF-α) -238G...

journal_title：Clinical chemistry and laboratory medicine

authors： Liaquat A,Shauket U,Ahmad W,Javed Q

更新日期：2015-02-01 00:00:00

abstract：:Method comparisons are indispensable tools for the extensive validation of analytic procedures. Laboratories often only want to know whether an established procedure (x-method) can be replaced by another one (y-method) without interfering with diagnostic purposes. Then split patients' samples are analyzed more or less...

journal_title：Clinical chemistry and laboratory medicine

authors： Haeckel R,Wosniok W,Al Shareef N

更新日期：2011-11-01 00:00:00

abstract：BACKGROUND:The study aim was to evaluate and compare the analytical performance of the new chemiluminescent immunoassay for cardiac troponin I (cTnI), called Access hs-TnI using DxI platform, with those of Access AccuTnI+3 method, and high-sensitivity (hs) cTnI method for ARCHITECT platform. METHODS:The limits of blan...

journal_title：Clinical chemistry and laboratory medicine

authors： Masotti S,Prontera C,Musetti V,Storti S,Ndreu R,Zucchelli GC,Passino C,Clerico A

更新日期：2018-02-23 00:00:00