Diagnostic performance of cerebrospinal fluid free light chains in Lyme neuroborreliosis - a pilot study.

Abstract:

:Background The aim of this study was to evaluate the diagnostic performance of cerebrospinal fluid (CSF) free light chains (FLCs) in the diagnosis of Lyme neuroborreliosis (LNB). Methods Serum and CSF levels of κ- and λ-FLC, albumin and total concentration of immunoglobulin M (IgM) were determined together with CSF chemokine CXCL13 in 23 patients with definite LNB, 35 inflammatory neurological disease control (INDC) and 18 non-inflammatory control (NIC) patients. Indices and intrathecal fractions (IFs) of FLC and IgM were calculated. Results Significant differences in FLC indices and IFs were found between the LNB group and both control groups, p ≤ 0.007. Sensitivity of intrathecal κ- and λ-FLC synthesis reached 78%-87% in LNB patients with a specificity of 94%-100% in NIC patients, whereas specificity in INDC patients was 69%. The corresponding frequencies of positive results for IF and index of IgM and CSF CXCL13 in these three diagnostic groups were 74%-96% in LNB patients, 0% in NIC patients and 3%-6% in INDC patients at the chosen cut-off levels. Conclusions The findings of this study show a moderate to high sensitivity of CSF κ- and λ-FLC in LNB patients with a high specificity in NIC patients. However, overlap in CSF κ- and λ-FLC levels between LNB and INDC patients calls for caution in the interpretation and limits the diagnostic usefulness in the LNB diagnosis. CSF CXCL13 appears to be the most valuable additional biomarker of LNB aside from routine parameters such as CSF pleocytosis and anti-Borrelia antibody index.

journal_name

Clin Chem Lab Med

authors

Tjernberg I,Johansson M,Henningsson AJ

doi

10.1515/cclm-2019-0315

subject

Has Abstract

pub_date

2019-11-26 00:00:00

pages

2008-2018

issue

12

eissn

1434-6621

issn

1437-4331

pii

/j/cclm.ahead-of-print/cclm-2019-0315/cclm-2019-03

journal_volume

57

pub_type

杂志文章
  • Plasma thiols and androgen levels in polycystic ovary syndrome.

    abstract::Homocysteine is a risk factor for ischemic heart disease; similarly as is hyperlipidemia or insulin resistance, which frequently occur in women with polycystic ovary syndrome. We examined the relationships between thiols and hormonal status or insulin resistance in 40 women (aged 25.8 +/- 7 years) with polycystic ovar...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2003.035

    authors: Vrbíková J,Tallová J,Biciková M,Dvoráková K,Hill M,Stárka L

    更新日期:2003-02-01 00:00:00

  • PATHFAST NT-proBNP (N-terminal-pro B type natriuretic peptide): a multicenter evaluation of a new point-of-care assay.

    abstract:BACKGROUND:The biochemical determination of cardiac natriuretic peptides, primarily brain natriuretic peptide (BNP) and the amino-terminal fragment of its pro-hormone proBNP (NT-proBNP), are reliable tools for diagnosing cardiac disease, establishing prognosis and evaluating the effectiveness of treatment. These biomar...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1515/CCLM.2010.222

    authors: Zaninotto M,Mion MM,Di Serio F,Caputo M,Ottomano C,Plebani M

    更新日期:2010-07-01 00:00:00

  • Drugs and brain death diagnostics: determination of drugs capable of inducing EEG zero line.

    abstract:BACKGROUND:Several drugs may affect the diagnosis of brain death by depressing the electroencephalographic signal. Serum levels of these drugs must be below their respective therapeutic ranges. METHODS:A high performance liquid chromatography-based fast and simple method was developed for determination of thiopentone,...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2008.335

    authors: Meinitzer A,Kalcher K,Gartner G,Halwachs-Baumann G,März W,Stettin M

    更新日期:2008-01-01 00:00:00

  • Evaluation of the first fully automated immunoassay method for the measurement of stimulating TSH receptor autoantibodies in Graves' disease.

    abstract:BACKGROUND:Thyroid stimulating hormone (TSH) receptor autoantibodies (TRAb) are pathogenetic and diagnostic hallmarks of Graves' disease (GD). Three varieties of TRAb have been described: stimulating (S-TRAb), blocking (B-TRAb), and apoptotic (A-TRAb) autoantibodies. Very recently, the first immunoassay method (Immulit...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2016-0197

    authors: Tozzoli R,D'Aurizio F,Villalta D,Giovanella L

    更新日期:2017-01-01 00:00:00

  • Comparison of functional fibrinogen (FF/CFF) and FIBTEM in surgical patients - a retrospective study.

    abstract:BACKGROUND:Fibrinogen-based clot firmness is reported as the maximum amplitude (MA) when using the citrated functional fibrinogen (CFF) assay in thrombelastography (TEG), and as the maximum clot firmness (MCF) together with several clot amplitude parameters when using the FIBTEM assay in thromboelastometry (ROTEM). Con...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2015-0345

    authors: Prüller F,Münch A,Preininger A,Raggam RB,Grinschgl Y,Krumnikl J,Toller W,Metzler H,Mahla E,Mangge H

    更新日期:2016-03-01 00:00:00

  • The integration of the detection of systemic sclerosis-associated antibodies in a routine laboratory setting: comparison of different strategies.

    abstract:BACKGROUND:Detection of systemic sclerosis-associated autoantibodies (SSc-Ab) is mostly restricted to anti-centromere and anti-topoisomerase-I. However, anti-RNA-polymerase-III and anti-PM/Scl are also important diagnostic markers for the disease supporting their incorporation in the laboratory repertoire. The aim of t...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2013-0211

    authors: Bonroy C,Smith V,Van Steendam K,Van Praet J,Deforce D,Devreese K,De Keyser F

    更新日期:2013-11-01 00:00:00

  • The Greek way to the Register: the establishment and operation of the Register for Clinical Chemists-Clinical Biochemists in Greece.

    abstract::In Greece, there is no officially organized training in clinical chemistry for scientists. The Greek Society of Clinical Chemistry-Clinical Biochemistry (GSCC-CB), following the encouragement of the EC4/RC decided to organize a voluntary Register for specialists in clinical chemistry. The following criteria for regist...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2011.687

    authors: Rizos D,Karababa P,Nikolou C,Sarandakou A,Panagiotakis O,Ferderigou A,Haliassos A,Makris K,Psarra K,Bairaktari E,Spyropoulou P,Galiatsatos N,Trakas N,Seferiadis K,Greek National Clinical Chemistry Registration Commission.

    更新日期:2011-08-30 00:00:00

  • Fucosylation of serum glycoproteins in lung cancer patients.

    abstract::Increased expression of sialyl Lewis X or A antigens on metastatic cancer cells leads to their selectin-mediated extravasation. Profound fucosylation of the serum microenvironment may be a factor that interrupts adhesion and influences the formation of metastases. In this study we quantitatively analyzed fucosylation ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2005.066

    authors: Kossowska B,Ferens-Sieczkowska M,Gancarz R,Passowicz-Muszyńska E,Jankowska R

    更新日期:2005-01-01 00:00:00

  • Impact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples.

    abstract:BACKGROUND:Blood biomarkers are subject to pre-analytical variability. In many cases, the stability of important new tissue biomarkers during freeze cycles and storage has not been studied sufficiently. METHODS:To test the stability of matrix metalloproteinases-7 (MMP-7) and their tissue inhibitors (TIMP-1), vascular ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2011.049

    authors: Kisand K,Kerna I,Kumm J,Jonsson H,Tamm A

    更新日期:2011-02-01 00:00:00

  • A combined index of cardiac biomarkers as a risk factor for early cardiovascular mortality in hemodialysis patients.

    abstract:BACKGROUND:Cardiac biomarkers, including cardiac troponin-I (cTn-I) and N-terminal pro brain natriuretic peptide (NT-proBNP) have been associated with poor outcome in hemodialysis (HD) patients. The present study was designed to evaluate these biomarkers as biological risk factors for early and late mortality in HD pat...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2012-0664

    authors: Bargnoux AS,Morena M,Jaussent I,Maurice F,Chalabi L,Leray-Moragues H,Terrier N,Dupuy AM,Badiou S,Canaud B,Cristol JP

    更新日期:2013-09-01 00:00:00

  • Is accuracy of serum free light chain measurement achievable?

    abstract::The serum free light chain (FLC) assay has proven to be an important complementary test in the management of patients with monoclonal gammopathies. The serum FLC assay has value for patients with plasma cell disorders in the context of screening and diagnosis, prognostic stratification, and quantitative monitoring. No...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 社论

    doi:10.1515/cclm-2015-0879

    authors: Jacobs JF,Tate JR,Merlini G

    更新日期:2016-06-01 00:00:00

  • Faecal haemoglobin concentrations vary with sex and age, but data are not transferable across geography for colorectal cancer screening.

    abstract:BACKGROUND:Faecal immunochemical tests (FIT) are becoming widely used in colorectal cancer (CRC) screening. Availability of data on faecal haemoglobin concentrations (f-Hb) in three countries prompted an observational study on sex and age and the transferability of data across geography. METHODS:Single estimates of f-...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2014-0115

    authors: Fraser CG,Rubeca T,Rapi S,Chen LS,Chen HH

    更新日期:2014-08-01 00:00:00

  • An association study of sodium-lithium countertransport activity with glutathione S transferase (GST) T1 and GST M1 null polymorphisms in Greek dyslipidaemic patients and controls.

    abstract:BACKGROUND:Previous genomic linkage studies have produced evidence linking sodium-lithium countertransport activity (Na/Li CT) with various chromosomal regions including loci harbouring glutathione S transferase (GST) genes. The aim of this study was to examine the putative association of erythrocyte Na/Li CT activity ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2008.065

    authors: Goulas A,Kosmidou M,Hatzitolios AI,Raikos N,Molyva D,Savopoulos C,Giannopoulos S,Fidani L

    更新日期:2008-01-01 00:00:00

  • Reference change values for lens culinaris agglutinin-reactive α-fetoprotein and des-γ-carboxy prothrombin in patients with chronic hepatitis C.

    abstract:BACKGROUND:Lens culinaris agglutinin-reactive α-fetoprotein (AFP-L3) and des-γ-carboxy prothrombin (DCP) have been routinely used as serological tumor markers of hepatocellular carcinoma (HCC) for surveillance. The aims of this study were: (i) to determine the biological variation of AFP-L3 and DCP in patients with chr...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2011-0912

    authors: Kanke F,Kumada T,Toyoda H,Satomura S

    更新日期:2012-03-06 00:00:00

  • Assessment of CA 15.3, CEA and TPA concentrations during monitoring of breast cancer.

    abstract::The variability of the tumor markers cancer antigen (CA) 15.3, carcinoembryonic antigen (CEA) and tissue polypeptide antigen (TPA) during steady state concentrations and the rate of increase during progression is described. One hundred and ninety-two patients were monitored during first-line chemotherapy for metastati...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2000.066

    authors: Sölétormos G,Petersen PH,Dombernowsky P

    更新日期:2000-05-01 00:00:00

  • Pediatric reference intervals for 29 Ortho VITROS 5600 immunoassays using the CALIPER cohort of healthy children and adolescents.

    abstract:BACKGROUND:Accurate reference intervals (RIs) based on a healthy pediatric population are essential for pediatric test result interpretation. The CALIPER project has recruited a large healthy cohort and completed a series of a priori studies to address gaps in pediatric RIs. As immunoassays from different manufacturers...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2017-0349

    authors: Higgins V,Fung AWS,Chan MK,Macri J,Adeli K

    更新日期:2018-01-26 00:00:00

  • Determination of the fatty acid profile of neutral lipids, free fatty acids and phospholipids in human plasma.

    abstract:BACKGROUND:Knowledge of the fatty acid composition of lipid classes in human plasma is an important factor in the investigation of human metabolism. Therefore, a method for the analysis of neutral lipid (NL), phospholipid (PL) and free fatty acids (FFA) in human plasma has been developed and validated. METHODS:Separat...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2012-0203

    authors: Firl N,Kienberger H,Hauser T,Rychlik M

    更新日期:2013-04-01 00:00:00

  • The PCSK9 gene E670G polymorphism affects low-density lipoprotein cholesterol levels but is not a risk factor for coronary artery disease in ethnic Chinese in Taiwan.

    abstract:BACKGROUND:An E670G polymorphism of the exon 12 of the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene was recently found to be associated with increased plasma low-density lipoprotein cholesterol (LDL-C) levels and severity of coronary atherosclerosis. This case-control study tested for a possible link betw...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2009.032

    authors: Hsu LA,Teng MS,Ko YL,Chang CJ,Wu S,Wang CL,Hu CF

    更新日期:2009-01-01 00:00:00

  • Distributions and ranges of values of blood and urinary biomarker of inflammation and oxidative stress in the workers engaged in office machine manufactures: evaluation of reference values.

    abstract:BACKGROUND:Interleukins, interferons and oxidative DNA products are important biomarkers assessing the inflammations and tissue damages caused by toxic materials in the body. We tried to evaluate distributions, reference values and age related changes of blood levels of inflammatory cytokines, C-reactive protein (CRP),...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2012-0330

    authors: Murase T,Kitamura H,Kochi T,Terunuma N,Kurosaki S,Hata K,Yanagi N,Uchino B,Kitahara K,Morimoto Y,Kasai H,Sasaki T,Ogami A,Higashi T

    更新日期:2013-02-01 00:00:00

  • The correlation between glucose fluctuation from self-monitored blood glucose and the major adverse cardiac events in diabetic patients with acute coronary syndrome during a 6-month follow-up by WeChat application.

    abstract::Background This study aimed to investigate the correlation between glucose fluctuation from self-monitored blood glucose (SMBG) and the major adverse cardiac events (MACE) in diabetic patients with acute coronary syndrome (ACS) during a 6-month follow-up period using the WeChat application. Methods From November 2016 ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2018-0220

    authors: Xia J,Hu S,Xu J,Hao H,Yin C,Xu D

    更新日期:2018-11-27 00:00:00

  • Hyperhomocysteinemia: association with renal transsulfuration and redox signaling in rats.

    abstract::Despite substantial evidence indicating the association of hyperhomocysteinemia (hHcys) and end-stage renal disease (ESRD), the pathogenic role of increased plasma homocysteine (Hcys) levels in the progression of ESRD remains unclear. This review will briefly summarize recent findings regarding the role of hHcys in th...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1515/CCLM.2007.344

    authors: Pin-Lan L,Fan Y,Ningjun L

    更新日期:2007-01-01 00:00:00

  • Lipoprotein lipase gene polymorphisms in Croatian patients with coronary artery disease.

    abstract::Modifications in lipoprotein lipase levels lead to elevated triglycerides and reduced high density lipoprotein (HDL), both of which are risk factors for coronary artery disease (CAD). Hence, we examined the influence of the -93T/G, D9N, N291S, and S447X polymorphisms in the lipoprotein lipase (LPL) gene on CAD risk an...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2003.082

    authors: Ferencak G,Pasalić D,Grsković B,Cheng S,Fijal B,Sesto M,Skodlar J,Rukavina AS

    更新日期:2003-04-01 00:00:00

  • Investigation on the ability of first trimester glycodelin and angiopoietin-2 to predict small-for-gestational age pregnancies at delivery.

    abstract:BACKGROUND:The aim was to investigate whether first trimester glycodelin and angiopoietin-2 can predict small-for-gestational age (SGA) at delivery, individually or in combination. METHODS:In this case-control study we measured glycodelin and angiopoietin-2 on serum from 170 singleton pregnant women delivering SGA neo...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2015-0989

    authors: Hansen YB,Myrhøj V,Jørgensen FS,Sørensen S

    更新日期:2016-09-01 00:00:00

  • Elevated levels of plasma homocysteine in postmenopausal women in Burkina Faso.

    abstract:BACKGROUND:Low levels of plasma homocysteine have been found in children and adult populations living in Burkina Faso in association with a low prevalence of coronary heart disease. METHODS:Based on this finding, the levels of plasma homocysteine and other thiols (cysteine, cysteinylglycine, glutathione) in postmenopa...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2005.131

    authors: Chillemi R,Simpore J,Persichilli S,Minucci A,D'Agata A,Musumeci S

    更新日期:2005-01-01 00:00:00

  • Soluble transferrin receptor and transferrin receptor-ferritin index in iron deficiency anemia and anemia in rheumatoid arthritis.

    abstract::The aim of the study was to evaluate the clinical efficiency of soluble transferrin receptor and transferrin receptor-ferritin index (sTfR/logF) in the diagnosis of iron deficiency anemia, as well as the differential diagnosis of iron deficiency anemia and anemia in rheumatoid arthritis. The study included 96 patients...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 临床试验,杂志文章

    doi:10.1515/CCLM.2005.056

    authors: Margetic S,Topic E,Ruzic DF,Kvaternik M

    更新日期:2005-01-01 00:00:00

  • Erythrocyte membrane acetylcholinesterase activity in subjects with MTHFR 677C-->T genotype.

    abstract:BACKGROUND:Increased homocysteine (Hcy) blood levels are correlated with vascular and neurological problems. AIM:The aim of the present study was to investigate erythrocyte membrane acetylcholinesterase (AChE) activity in subjects with the MTHFR C677T genotype in relation to Hcy. METHODS:Blood was obtained from 22 in...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2006.005

    authors: Tsakiris S,Schulpis KH,Papaconstantinou ED,Tsakiris T,Tjamouranis I,Giannoulia-Karantana A

    更新日期:2006-01-01 00:00:00

  • Comparison of Improvacuter™ tubes with BD Vacutainer™ tubes for various hormones in the aspects of stability and influence of gel separators.

    abstract:BACKGROUND:Validation of blood collection tubes are important to determine the role of different collection tubes which influence the assurance of laboratory results. We compared two different tubes (Improvacuter™ and Becton Dickinson [BD] Vacutainer™) and investigated the effect of gel and storage time in comparison w...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2014-0583

    authors: Zungun C,Yılmaz FM,Boru EG,Topcuoglu C

    更新日期:2015-02-01 00:00:00

  • Diagnostic testing for a high-grade inflammation: parameter dynamics and novel markers.

    abstract:BACKGROUND:The timely detection of severe inflammatory conditions is of great importance for early therapy initiation and the patient's prognosis. The complex humoral and cellular processes involved in inflammation offer many opportunities for diagnostic testing, which are still unused in clinical practice. We investig...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2014-0482

    authors: Hoffmann C,Hoffmann P,Zimmermann M

    更新日期:2015-03-01 00:00:00

  • The high-sensitivity cardiac troponin T assay is superior to its previous assay generation for prediction of 90-day clinical outcome in ischemic stroke.

    abstract:BACKGROUND:Cardiac troponin T (cTnT) has been reported to be a predictor of stroke outcome. We hypothesized that the high-sensitivity cTnT (hs-cTnT) assay is superior to its previous assay generation for stroke outcome prediction. METHODS:cTnT was measured on emergency department admission in 60 consecutive patients (...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/cclm-2012-0176

    authors: Furtner M,Ploner T,Hammerer-Lercher A,Pechlaner R,Mair J

    更新日期:2012-11-01 00:00:00

  • IFCC handbook on Master Program in clinical laboratory sciences.

    abstract::This document summarizes the steps in development of a new IFCC Master Program and outlines some of the ways the IFCC would assist in setting up such a regional Program. This is intended to be of assistance to those who wish to embark on such an endeavour. ...

    journal_title:Clinical chemistry and laboratory medicine

    pub_type: 杂志文章

    doi:10.1515/CCLM.2003.212

    authors: Fink NE,Allen LC,International Federation of Clinical Chemistry and Laboratory Medicine.,IFCC Committee on Education and Curriculum Development, Education and Management Division.

    更新日期:2003-10-01 00:00:00