Using the hemoglobin-binding Staphylococcus aureus protein IsdH to enable plasma analysis of hemolyzed blood samples.

abstract：:The commutability of 13 control materials was evaluated by performing parallel measurements on two different analysers: a Synchron CX-5 Delta from Beckman-Coulter and a Vitros 950 from Ortho-Clinical Diagnostics. Twenty three clinical chemistry analytes (substrates, electrolytes and enzymatic activities) were determin...

journal_title：Clinical chemistry and laboratory medicine

authors： Brion E,Lessinger JM,Gould N,Leyendecker J,Férard G

更新日期：2002-06-01 00:00:00

abstract：:An outbreak of the so-called Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2), has been spreading rapidly nationwide in China since December 2019. Wuhan, Hubei Province, is the hardest-hit region, with a rise in confirmed cases and its hospitals overwhelme...

journal_title：Clinical chemistry and laboratory medicine

authors： Yusong L,Duanliang P,Jia Y,Dunyan C,Hongbing J,Siyuan Y,Huanhuan C,Juan Z,Lyurong L

更新日期：2020-06-25 00:00:00

abstract：:Cryoglobulins are proteins that precipitate at temperatures below 37 degrees C. Cold-induced precipitation of proteins may occur in vivo secondary to several important diseases, and lead to pathological manifestations involving different organs. Cryoprecipitation may be observed in vitro by exposing serum samples, sup...

journal_title：Clinical chemistry and laboratory medicine

authors： Di Stasio E,Bizzarri P,Bove M,Casato M,Giardina B,Fiorilli M,Galtieri A,Pucillo LP

更新日期：2003-02-01 00:00:00

abstract：:The variability of the tumor markers cancer antigen (CA) 15.3, carcinoembryonic antigen (CEA) and tissue polypeptide antigen (TPA) during steady state concentrations and the rate of increase during progression is described. One hundred and ninety-two patients were monitored during first-line chemotherapy for metastati...

journal_title：Clinical chemistry and laboratory medicine

authors： Sölétormos G,Petersen PH,Dombernowsky P

更新日期：2000-05-01 00:00:00

abstract：BACKGROUND:In large clinical trials, where RNA cannot be extracted immediately after sampling, preserving RNA in whole blood is a crucial initial step in obtaining robust qPCR data. The current golden standard for RNA preservation is costly and designed for time-consuming column-based RNA-extraction. We investigated th...

journal_title：Clinical chemistry and laboratory medicine

authors： Mærkedahl RB,Frøkiær H,Lauritzen L,Metzdorff SB

更新日期：2015-07-01 00:00:00

abstract：:For several years it has been possible to routinely detect numerous mutations in the human genome by different methods. The most common technique is a standard PCR, but real time fluorescence PCR is increasingly being used. The purpose of this paper is to compare these two different techniques from the point of view o...

journal_title：Clinical chemistry and laboratory medicine

authors： Schroell-Metzger B,Dicato M,Bosseler M,Berchem G

更新日期：2003-04-01 00:00:00

abstract：:Despite substantial evidence indicating the association of hyperhomocysteinemia (hHcys) and end-stage renal disease (ESRD), the pathogenic role of increased plasma homocysteine (Hcys) levels in the progression of ESRD remains unclear. This review will briefly summarize recent findings regarding the role of hHcys in th...

journal_title：Clinical chemistry and laboratory medicine

authors： Pin-Lan L,Fan Y,Ningjun L

更新日期：2007-01-01 00:00:00

abstract：OBJECTIVE:Plasma non-esterified (free) fatty acid concentrations rise as a consequence of stimulated endogenous lipolysis and are inversely related to the plasma magnesium concentration when plasma adrenaline concentration is increased. The aim of the study was to test whether high plasma non-esterified fatty acid conc...

journal_title：Clinical chemistry and laboratory medicine

authors： de Valk HW,Bianchi R,van Rijn HJ,Erkelens DW

更新日期：1998-02-01 00:00:00

abstract：BACKGROUND:Haptoglobin (Hp) is an abundant serum protein which binds extracorpuscular hemoglobin (Hb). Two alleles exist in humans for the Hp gene, denoted 1 and 2. Diabetic individuals with the Hp 2-2 genotype are at increased risk of developing vascular complications including heart attack, stroke, and kidney disease...

journal_title：Clinical chemistry and laboratory medicine

authors： Levy NS,Vardi M,Blum S,Miller-Lotan R,Afinbinder Y,Cleary PA,Paterson AD,Bharaj B,Snell-Bergeon JK,Rewers MJ,Lache O,Levy AP

更新日期：2013-08-01 00:00:00

abstract：:Increased expression of sialyl Lewis X or A antigens on metastatic cancer cells leads to their selectin-mediated extravasation. Profound fucosylation of the serum microenvironment may be a factor that interrupts adhesion and influences the formation of metastases. In this study we quantitatively analyzed fucosylation ...

journal_title：Clinical chemistry and laboratory medicine

authors： Kossowska B,Ferens-Sieczkowska M,Gancarz R,Passowicz-Muszyńska E,Jankowska R

更新日期：2005-01-01 00:00:00

abstract：:Individual risk of toxicity or cancer reflects the amount of exposure to environmental agents, combined with one's underlying genetic predisposition. More than six dozen human ecogenetic polymorphisms have been described; whereas some of these have been demonstrated to be associated with altered risks of toxicity or c...

journal_title：Clinical chemistry and laboratory medicine

authors： Nebert DW

更新日期：2000-09-01 00:00:00

abstract：:Prolidases I and II were highly purified from human erythrocytes. The effects of various amino acids, MnCl2 and mercaptoethanol, on these two enzymes were investigated. Normal prolidase II was very labile in the absence of MnCl2 or mercaptoethanol. The activity of prolidase II was maintained at about 76% by pre-incuba...

journal_title：Clinical chemistry and laboratory medicine

authors： Nakayama K,Awata S,Zhang J,Kaba H,Manabe M,Kodama H

更新日期：2003-10-01 00:00:00

abstract：BACKGROUND:The long noncoding RNAs (lncRNAs) have gradually been reported to be an important class of RNAs with pivotal roles in regulation of gene expression, and thus are involved in multitudinous human complex diseases. However, the biological functions and precise mechanisms of the majority of lncRNAs are still poo...

journal_title：Clinical chemistry and laboratory medicine

authors： Zhuo Y,Zeng Q,Zhang P,Li G,Xie Q,Cheng Y

更新日期：2017-01-01 00:00:00

abstract：:The aim of this multicenter study was to evaluate the technical and clinical performance of the Elecsys S100 electrochemiluminescence immunoassay and to assess its utility as a tumor marker in patients with malignant melanoma. Imprecision studies yielded within-run coefficients of variation (CVs) of 0.7-2.0% and betwe...

journal_title：Clinical chemistry and laboratory medicine

authors： Alber B,Hein R,Garbe C,Caroli U,Luppa PB

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Vitamin B12 deficiency and bone fractures are common in vegetarians. However, a direct relationship between vitamin B12 status and bone metabolism in vegetarians has not been tested sufficiently. METHODS:Our study included 96 vegetarians (23 German vegans, and 54 German and 19 Indian lacto-, lacto-ovo-veget...

journal_title：Clinical chemistry and laboratory medicine

authors： Herrmann W,Obeid R,Schorr H,Hübner U,Geisel J,Sand-Hill M,Ali N,Herrmann M

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Crohn's disease (CD) is an incurable and difficult to diagnose condition. While high sensitive C-reactive protein (CRP) remains the best biochemical marker, we evaluated the diagnostic usefulness of lipid peroxidation indices. METHODS:Malondialdehyde/thiobarbituric acid-reactive substances (MDA/TBARS), pero...

journal_title：Clinical chemistry and laboratory medicine

authors： Boehm D,Krzystek-Korpacka M,Neubauer K,Matusiewicz M,Paradowski L,Gamian A

更新日期：2012-03-03 00:00:00

abstract：:The early diagnosis of rheumatoid arthritis (RA) has become a priority owing to the availability of effective disease-modifying agents that can improve patient wellbeing and influence the clinical outcome. However, this represents a real challenge, as no clinical, radiological or immunological features are pathognomon...

journal_title：Clinical chemistry and laboratory medicine

authors： Bizzaro N

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Extensive application of measurement of urinary iodine concentration (UIC) in several benign and malignant thyroid diseases could profit by the availability of rapid and inexpensive measuring techniques. Aim of this study was to apply a simple and inexpensive commercially available potentiometric method for ...

journal_title：Clinical chemistry and laboratory medicine

authors： Giovacchini G,Giovanella L,Haldemann A,Staub U,Füchsel FG,Koch P

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND:Apolipoprotein E (APOE) and choline acetyltransferase (ChAT) have been suggested as candidate genes for determining the risk of late-onset Alzheimer's disease. The aim of this study was to simultaneously detect polymorphisms in codons 112 and 158 of APOE and codon 2 of ChAT by hybridization probe multiplexin...

journal_title：Clinical chemistry and laboratory medicine

authors： Park HD,Park KU,Kim KW,Song J,Chang HE,Heo SR,Lee HJ,Kim JQ

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The use of laboratory tests has been increasing in recent years due to various factors affecting laboratories, physicians, legal aspects, or patients themselves. METHODS:The efficacy of laboratory tests must be evaluated on the basis of the clinical benefits that they provide in terms of prevention, diagnos...

journal_title：Clinical chemistry and laboratory medicine

authors： Alonso-Cerezo MC,Martín JS,García Montes MA,de la Iglesia VM

更新日期：2009-01-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are genetic multisystemic diseases due to various defects in the biosynthesis or processing of glycoproteins. Our aim is to present our experience in the selective screening of CDG syndrome in a paediatric population (421 patients) with clinical suspicion of the disease, ana...

journal_title：Clinical chemistry and laboratory medicine

authors： Colomé C,Ferrer I,Artuch R,Vilaseca MA,Pineda M,Briones P

更新日期：2000-10-01 00:00:00

abstract：:The results of several recent experimental studies using animal models and clinical trials suggested that obesity is not merely an epiphenomenon or a prominent comorbidity in patients with heart failure (HF). Indeed, recent studies suggest that obesity is intimately involved in the pathogenesis of HF with preserved ej...

journal_title：Clinical chemistry and laboratory medicine

authors： Clerico A,Zaninotto M,Passino C,Plebani M

更新日期：2018-06-27 00:00:00

abstract：:Until recently, the "human genome" programs were mainly directed towards the development of maps to identify disease genes. The genetic map comprises about 8000 highly informative second generation markers of the microsatellite type. The density of markers is now sufficient to localize a gene for a monogenic disease w...

journal_title：Clinical chemistry and laboratory medicine

authors： Weissenbach J

更新日期：1998-08-01 00:00:00

abstract：BACKGROUND:A crucial question when developing reference intervals is whether different subpopulations need their own reference interval or if a single joint reference interval can be used. It is reasonable to use partitioned reference intervals in situations where a single interval results in considerable variation in ...

journal_title：Clinical chemistry and laboratory medicine

authors： Gellerstedt M,Petersen PH

更新日期：2007-01-01 00:00:00

abstract：:Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired disorder of the pluripotent stem cell resulting from the somatic mutation of the X-linked PIG-A gene, involved in the synthesis of the glycosylphosphatidyl-inositol anchor of membrane proteins such as CD55, CD59 and CD14. In the past decade, flow cytometry has b...

journal_title：Clinical chemistry and laboratory medicine

authors： Hevessy Z,Nagy B Jr,Kiss F,Kiss A,Kappelmayer J

更新日期：2005-01-01 00:00:00

abstract：:Although affinity and kinetic measurements on their own provide useful information regarding the suitability of antibodies for various immunoassays, a thermodynamic analysis provices additional information that throws light on the molecular forces at work in the antigen-antibody interaction. It may then be possible to...

journal_title：Clinical chemistry and laboratory medicine

authors： Van Regenmortel MH

更新日期：1998-06-01 00:00:00

abstract：BACKGROUND:Despite remarkable advances in technology and laboratory automation, results of laboratory testing still suffer from a high degree of preanalytical variability. Although there is no definitive evidence, the use of small-gauge needles for venipuncture is usually discouraged to reduce the chance of producing u...

journal_title：Clinical chemistry and laboratory medicine

authors： Lippi G,Salvagno GL,Montagnana M,Brocco G,Cesare Guidi G

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Faecal immunochemical tests (FIT) are becoming widely used in colorectal cancer screening. Estimation of faecal haemoglobin concentration in a large group prompted an observational study on gender and age. METHODS:A single estimate of faecal haemoglobin concentration was made using quantitative automated im...

journal_title：Clinical chemistry and laboratory medicine

authors： McDonald PJ,Strachan JA,Digby J,Steele RJ,Fraser CG

更新日期：2011-12-07 00:00:00

abstract：:The aim of the study was to investigate whether procalcitonin, soluble CD14 and interleukin-6 show advantages in predicting the outcome and specificity for bacterial infection in patients with sepsis in comparison to common C-reactive protein measurement. Laboratory parameters were measured in plasma of patients durin...

journal_title：Clinical chemistry and laboratory medicine

authors： Herrmann W,Ecker D,Quast S,Klieden M,Rose S,Marzi I

更新日期：2000-01-01 00:00:00

abstract：:Experimental and observational studies support a role of plasma homocysteine levels (tHcy) in coronary artery disease (CAD). In the GENICA (Genetic and Environmental factors In Coronary Atherosclerosis) study, we found that high tHcy predicted cardiovascular mortality in hypertensive, but not in normotensive, patients...

journal_title：Clinical chemistry and laboratory medicine

authors： Rossi GP,Seccia TM,Pessina AC

更新日期：2007-01-01 00:00:00