Abstract:
BACKGROUND:Anti-cyclic citrullinated peptide (CCP) antibody is emerging as an important diagnostic marker for rheumatoid arthritis (RA). We evaluated the analytical and diagnostic performance of the ARCHITECT anti-CCP (Abbott Diagnostics), a new fully automated chemiluminescent microparticle immunoassay. METHODS:Serum samples from 69 patients with RA and 86 non-RA patients were used to evaluate the performance of the ARCHITECT anti-CCP assay, and the results were compared with those of EliA CCP (Phadia). The optimal cut-off value was calculated using receiver operating characteristic (ROC) curve analysis. RESULTS:Within-run and total imprecision (%CV) of the ARCHITECT anti-CCP were <6% and good linearity was observed over the claimed range. The areas under the ROC curves for the ARCHITECT anti-CCP and EliA CCP were 0.90 and 0.89, respectively. The sensitivity and specificity were 76.8% and 95.3% for the ARCHITECT anti-CCP and 78.3% and 95.3% for EliA CCP using the manufacturer's cut-off thresholds. Both assays showed sensitivity of 84.1% and specificity of 94.2% using the optimal cut-off values. CONCLUSIONS:The analytical performance of the ARCHITECT anti-CCP was satisfactory and diagnostic performance was comparable to that of EliA CCP. The use of optimal cut-off thresholds can yield higher sensitivity with minimal loss of specificity.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Hwang SM,Kim JO,Yoo YM,Shin S,Kim JQ,Park MH,Song EYdoi
10.1515/CCLM.2010.046subject
Has Abstractpub_date
2010-02-01 00:00:00pages
225-30issue
2eissn
1434-6621issn
1437-4331journal_volume
48pub_type
杂志文章abstract:BACKGROUND:An E670G polymorphism of the exon 12 of the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene was recently found to be associated with increased plasma low-density lipoprotein cholesterol (LDL-C) levels and severity of coronary atherosclerosis. This case-control study tested for a possible link betw...
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doi:10.1515/CCLM.2003.040
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,多中心研究
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更新日期:2014-11-01 00:00:00
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更新日期:2005-01-01 00:00:00
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更新日期:2008-01-01 00:00:00