Abrupt climate changes during Termination III in Southern Europe.

abstract：:Histone methyltransferase G9a has critical roles in promoting cancer-cell growth and gene suppression, but whether it is also associated with the DNA damage response is rarely studied. Here, we report that loss of G9a impairs DNA damage repair and enhances the sensitivity of cancer cells to radiation and chemotherapeu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Q,Zhu Q,Lu X,Du Y,Cao L,Shen C,Hou T,Li M,Li Z,Liu C,Wu D,Xu X,Wang L,Wang H,Zhao Y,Yang Y,Zhu WG

更新日期：2017-07-25 00:00:00

abstract：:The addition of insulin to a mixture of plasma membrane and mitochondrial fractions from rat adipocytes results in a decrease in the phosphorylation of a mitochondrial protein identified as the alpha subunit of pyruvate dehydrogenase [pyruvate:lipoamide oxidoreductase (decarboxylating and acceptor-acetylating), EC 1.2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seals JR,Jarett L

更新日期：1980-01-01 00:00:00

abstract：:When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myers JC,Dobkin C,Spiegelman S

更新日期：1980-03-01 00:00:00

abstract：:In the manufacture of chemical feedstocks and subsequent processing into derivatives and materials, the U.S. chemical industry sets the current standard of excellence for technological competitiveness. This world-class leadership is attributed to the innovation and advancement of chemical engineering process technolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell JW

更新日期：1992-02-01 00:00:00

abstract：:High-resolution maps integrated with the enhanced location data base software (LDB+) give improved estimates of genetic parameters and reveal characteristics of cytogenetic bands. Chiasma interference is intermediate between Kosambi and Carter-Falconer levels, as in Drosophila and the mouse. The autosomal genetic map ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins A,Frezal J,Teague J,Morton NE

更新日期：1996-12-10 00:00:00

abstract：:A hallmark of most neurodegenerative diseases, including those caused by polyglutamine expansion, is the formation of ubiquitin (Ub)-positive protein aggregates in affected neurons. This finding suggests that the Ub system may be involved in common mechanisms underlying these otherwise unrelated diseases. Here we repo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donaldson KM,Li W,Ching KA,Batalov S,Tsai CC,Joazeiro CA

更新日期：2003-07-22 00:00:00

abstract：:Early conformational events during folding of acyl-CoA binding protein (ACBP), an 86-residue alpha-helical protein, were explored by using a continuous-flow mixing apparatus with a dead time of 70 micros to measure changes in intrinsic tryptophan fluorescence and tryptophan-dansyl fluorescence energy transfer. Althoug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teilum K,Maki K,Kragelund BB,Poulsen FM,Roder H

更新日期：2002-07-23 00:00:00

abstract：:Cell fusion was induced by brain extracts containing the scrapie virus and the virus of Creutzfeldt-Jakob disease. The assay involved quantitation of colony-forming ability in a double selection system, strandardized against fusion induced by Sendai virus. Correlation between the logarithm of virus dilution and the hy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kidson C,Moreau MC,Asher DM,Brown PW,Coon HG,Gajdusek DC,Gibbs CJ Jr

更新日期：1978-06-01 00:00:00

abstract：:The physiological effects of nitroglycerin as a potent vasodilator have long been documented. However, the molecular mechanisms by which nitroglycerin exerts its biological functions are still a matter of intense debate. Enzymatic pathways converting nitroglycerin to vasoactive compounds have been identified, but none...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonini MG,Stadler K,Silva SO,Corbett J,Dore M,Petranka J,Fernandes DC,Tanaka LY,Duma D,Laurindo FR,Mason RP

更新日期：2008-06-24 00:00:00

abstract：:The crossed strand-exchange form (Holliday structure, half chiasma) has been predicted as an intermediate in the genetic recombination of eukaryotes. We report here the detection of this form in the yeast plasmid, 2-micron DNA, isolated during meiosis. Physical mapping has previously suggested that two forms of 2-micr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bell L,Byers B

更新日期：1979-07-01 00:00:00

abstract：:We report a signal-on, electronic DNA (E-DNA) sensor that is label-free and achieves a subpicomolar detection limit. The sensor, which is based on a target-induced strand displacement mechanism, is composed of a "capture probe" attached by its 5' terminus to a gold electrode and a 5' methylene blue-modified "signaling...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao Y,Lubin AA,Baker BR,Plaxco KW,Heeger AJ

更新日期：2006-11-07 00:00:00

abstract：:Major histocompatibility complex (MHC) class I multimer technology has become an indispensable immunological assay system to dissect antigen-specific cytotoxic CD8(+) T cell responses by flow cytometry. However, the development of high-throughput assay systems, in which T cell responses against a multitude of epitopes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bakker AH,Hoppes R,Linnemann C,Toebes M,Rodenko B,Berkers CR,Hadrup SR,van Esch WJ,Heemskerk MH,Ovaa H,Schumacher TN

更新日期：2008-03-11 00:00:00

abstract：:In Drosophila, heterochromatin protein 1 (HP1) suppresses the expression of euchromatic genes that are artificially translocated adjacent to heterochromatin by expanding heterochromatin structure into neighboring euchromatin. The purpose of this study was to determine whether HP1 functions as a transcriptional repress...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang KK,Eissenberg JC,Worman HJ

更新日期：2001-09-25 00:00:00

abstract：:M cells located in the follicle-associated epithelium of Peyer's patches (PP) are shown to be the principal sites for the sampling of gut luminal antigens. Thus, PP have long been considered the gatekeepers of the mucosal immune system. Here, we report a distinct gateway for the uptake of gut bacteria: clusters of non...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jang MH,Kweon MN,Iwatani K,Yamamoto M,Terahara K,Sasakawa C,Suzuki T,Nochi T,Yokota Y,Rennert PD,Hiroi T,Tamagawa H,Iijima H,Kunisawa J,Yuki Y,Kiyono H

更新日期：2004-04-20 00:00:00

abstract：:A critique is presented of the Lumsden-Wilson theory [Lumsden, C. J. & Wilson, E. O. (1980) Proc. Natl. Acad. Sci. USA 77, 4382-4386] of the transmission of cultural traits. An analysis of the underlying assumptions and the mathematical nature of the theory clarifies its essentially reductionist and determinist qualit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alper JS,Lange RV

更新日期：1981-06-01 00:00:00

abstract：:The affinity and stoichiometry of DNA binding by Escherichia coli trp repressor were studied by electrophoresis in nondenaturing gels. The ability of trp repressor to retard the electrophoretic mobility of an operator DNA fragment depends on the pH of the gel system. Above the pI of the protein, little retardation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carey J

更新日期：1988-02-01 00:00:00

abstract：:In order to determine the chemical features of human placental lactogen (hPL) necessary for its biologic activity we prepared the following fragments from the plasmin-cleaved hormone: reduced and alkylated 1--134, reduced and alkylated 141--191, and a 1--134 dimer joined through the single cysteinyl residue at positio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russell J,Sherwood LM,Kowalski K,Schneider AB

更新日期：1979-03-01 00:00:00

abstract：:Water oxidation by cyanobacteria, algae, and plants is pivotal in oxygenic photosynthesis, the process that powers life on Earth, and is the paradigm for engineering solar fuel-production systems. Each complete reaction cycle of photosynthetic water oxidation requires the removal of four electrons and four protons fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klauss A,Haumann M,Dau H

更新日期：2012-10-02 00:00:00

abstract：:It is suggested that differentiated embryonic cells have a high specificity of molecular constitution as regards the surface layers surrounding their cellular membranes. Correspondingly, specific interface energies may characterize the early contacts between different cell types. The question is raised whether the mor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jehle H

更新日期：1970-09-01 00:00:00

abstract：:Recent studies indicate that impairments in two cognitive domains characterize the cognitive abnormalities that appear earliest in the course of Alzheimer disease (AD). These cognitive domains pertain to memory and executive function ability; in particular, memory test scores reflecting the difference between immediat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albert MS

更新日期：1996-11-26 00:00:00

abstract：:We have developed a reliable genetic selection strategy for isolating interacting proteins based on the "hitchhiker" mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway. This method, designated FLI-TRAP (functional ligand-binding identification by Tat-based recognition of associating proteins),...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waraho D,DeLisa MP

更新日期：2009-03-10 00:00:00

abstract：:Helminthosporoside is a host-specific toxin produced by Helminthosporium sacchari, both in culture and in infected sugarcane tissue. The susceptibility of any given clone of sugarcane to disease is invariably associated with the presence of a toxin-binding protein. This report presents evidence for the presence of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strobel GA,Hess WM

更新日期：1974-04-01 00:00:00

abstract：:The Escherichia coli cytosolic homotetrameric protein SecB is known to be involved in protein export across the plasma membrane. A currently prevalent view holds that SecB functions exclusively as a chaperone interacting nonspecifically with unfolded proteins, not necessarily exported proteins, whereas a contrary view...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe M,Blobel G

更新日期：1995-10-24 00:00:00

abstract：:Inactivation of the p53 pathway represents the most common molecular defect of human cancer. But in the setting of melanoma, a highly aggressive and invariably fatal malignancy in its advanced disseminated form, mutation/deletion of p53 is relatively rare, whereas its positive regulator ARF is often lost. Here, we sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ha L,Ichikawa T,Anver M,Dickins R,Lowe S,Sharpless NE,Krimpenfort P,Depinho RA,Bennett DC,Sviderskaya EV,Merlino G

更新日期：2007-06-26 00:00:00

abstract：:By affinity chromatography on heparin-Sepharose, two classes of lipoproteins were separated from high density lipoproteins (HDL) isolated from patients with primary or secondary lecithin:cholesterol acyltransferase (LCATase; EC 2.3.1.43) deficiency and from normal subjects. The unretained fraction, HDL(A), was charact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marcel YL,Vezina C,Emond D,Suzue G

更新日期：1980-05-01 00:00:00

abstract：:The assembly of regional biotas and organismal responses to anthropogenic climate change both depend on the capacity of organisms to adapt to novel ecological conditions. Here we demonstrate the concept of evolutionary lag time, the time between when a climatic regime or habitat develops in a region and when it is col...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guerrero PC,Rosas M,Arroyo MT,Wiens JJ

更新日期：2013-07-09 00:00:00

abstract：:The heat shock protein (Hsp)70 family of molecular chaperones interacts with unfolded proteins through a C-terminal substrate-binding domain (SBD) that is controlled by nucleotide binding to the N-terminal domain. The ATPase cycle is regulated by cochaperones, including DnaJ proteins that accelerate ATP hydrolysis to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Awad W,Estrada I,Shen Y,Hendershot LM

更新日期：2008-01-29 00:00:00

abstract：:We previously reconstituted Z rings in tubular multilamellar liposomes with FtsZ-YFP-mts, where mts is a membrane-targeting amphiphilic helix. These reconstituted Z rings generated a constriction force but did not divide the thick-walled liposomes. Here we developed a unique system to observe Z rings in unilamellar li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Osawa M,Erickson HP

更新日期：2013-07-02 00:00:00

abstract：:Maintenance of genomic stability is essential for normal organismal development and is vital to prevent diseases such as cancer. As genetic information is packaged into chromatin, it has become increasingly clear that the chromatin environment plays an important role in DNA damage response. However, how DNA repair is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Liu L,Yang S,Song N,Zhou X,Gao J,Yu N,Shan L,Wang Q,Liang J,Xuan C,Wang Y,Shang Y,Shi L

更新日期：2014-05-13 00:00:00

abstract：:Future nuclear arms reduction efforts will require technologies to verify that warheads slated for dismantlement are authentic without revealing any sensitive weapons design information to international inspectors. Despite several decades of research, no technology has met these requirements simultaneously. Recent wor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vavrek JR,Henderson BS,Danagoulian A

更新日期：2018-04-24 00:00:00