Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilation.

abstract：:Tetrahymena ribozymes hold promise for repairing genetic disorders but are largely limited by their modest splicing efficiency and low production of final therapeutic proteins. Ribozyme evolution in intact living mammalian cells would greatly facilitate the discovery of new ribozyme variants with high in vivo activity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasegawa S,Jackson WC,Tsien RY,Rao J

更新日期：2003-12-09 00:00:00

abstract：:Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the gene encoding MeCP2. By binding to methylated CpG dinucleotide promoter regions, MeCP2 acts as a transcriptional repressor, predicting that its absence might result in widespread aberrant gene transcription, leading to the RTT phenotype. C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matarazzo V,Ronnett GV

更新日期：2004-05-18 00:00:00

abstract：:The brain continuously influences and perceives the physiological condition of the body. Related cortical representations have been proposed to shape emotional experience and guide behavior. Although previous studies have identified brain regions recruited during autonomic processing, neurological lesion studies have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo CC,Sturm VE,Zhou J,Gennatas ED,Trujillo AJ,Hua AY,Crawford R,Stables L,Kramer JH,Rankin K,Levenson RW,Rosen HJ,Miller BL,Seeley WW

更新日期：2016-04-26 00:00:00

abstract：:The human beta(2)-adrenergic receptor gene has multiple single-nucleotide polymorphisms (SNPs), but the relevance of chromosomally phased SNPs (haplotypes) is not known. The phylogeny and the in vitro and in vivo consequences of variations in the 5' upstream and ORF were delineated in a multiethnic reference populatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drysdale CM,McGraw DW,Stack CB,Stephens JC,Judson RS,Nandabalan K,Arnold K,Ruano G,Liggett SB

更新日期：2000-09-12 00:00:00

abstract：:Strongylocentrotus intermedius (A. Agassiz, 1863) is an economically important sea urchin inhabiting the northwest Pacific region of Asia. The northern Primorye (Sea of Japan) populations of S. intermedius consist of two sympatric morphological forms, "usual" (U) and "gray" (G). The two forms are significantly differe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balakirev ES,Pavlyuchkov VA,Ayala FJ

更新日期：2008-10-21 00:00:00

abstract：:Natural killer (NK) cells provide in vivo control of orthopoxvirus infections in association with their expansion in the draining lymph node (LN), where they are normally very rare. The mechanism of this expansion is unclear. Herein, we determined that NK-cell depletion results in enhanced infection following footpad ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pak-Wittel MA,Yang L,Sojka DK,Rivenbark JG,Yokoyama WM

更新日期：2013-01-02 00:00:00

abstract：:Neonates are highly susceptible to infectious diseases and, in general, respond poorly to conventional vaccines due to immaturity of the immune system. In the present study, we hypothesized that the anti-tetanus toxoid (TT) vaccine response of neonatal mice could be enhanced by retinoic acid (RA), a bioactive retinoid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Y,Ross AC

更新日期：2005-09-20 00:00:00

abstract：:Host-seeking behavior by parasitic nematodes relies heavily on chemical cues emanating from potential hosts. Nonspecific cues for Strongyloides stercoralis, a nematode that infects humans and a few other mammals, include carbon dioxide and sodium chloride; however, the characteristic species specificity of this parasi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Safer D,Brenes M,Dunipace S,Schad G

更新日期：2007-01-30 00:00:00

abstract：:Electron transfer dissociation (ETD) is a recently introduced mass spectrometric technique that provides a more comprehensive coverage of peptide sequences and posttranslational modifications. Here, we evaluated the use of ETD for a global phosphoproteome analysis. In all, we identified a total of 1,435 phosphorylatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Molina H,Horn DM,Tang N,Mathivanan S,Pandey A

更新日期：2007-02-13 00:00:00

abstract：:Nitrogen metabolism in bacteria and archaea is regulated by a ubiquitous class of proteins belonging to the P(II)family. P(II) proteins act as sensors of cellular nitrogen, carbon, and energy levels, and they control the activities of a wide range of target proteins by protein-protein interaction. The sensing mechanis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rajendran C,Gerhardt EC,Bjelic S,Gasperina A,Scarduelli M,Pedrosa FO,Chubatsu LS,Merrick M,Souza EM,Winkler FK,Huergo LF,Li XD

更新日期：2011-11-22 00:00:00

abstract：:Four synthetic peptides that together constitute the cell attachment domain of fibronectin [Pierschbacher, M.D., Ruoslahti, E., Sundelin, J., Lind, P. & Peterson, P. (1982) J. Biol. Chem. 257, 9593-9597] were constructed and tested for their ability to induce cell attachment and spreading. One of these peptides, consi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pierschbacher M,Hayman EG,Ruoslahti E

更新日期：1983-03-01 00:00:00

abstract：:The up-regulation of the 25-hydroxyvitamin D(3)-24-hydroxylase by 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] is well established and occurs at the transcriptional level through two vitamin D response elements in the promoter of the gene. However, the mechanism of down-regulation of the 24-hydroxylase by parathyroid ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zierold C,Mings JA,DeLuca HF

更新日期：2001-11-20 00:00:00

abstract：:A direct method for the determination of N-linked glycosylation sites in highly glycosylated proteins is described. Carcinoembryonic antigen (CEA) and a nonspecific crossreacting antigen (NCA) were chemically deglycosylated, and peptide maps were prepared by reverse-phase HPLC. The peptides were sequenced on a gas-pha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paxton RJ,Mooser G,Pande H,Lee TD,Shively JE

更新日期：1987-02-01 00:00:00

abstract：:The midcycle luteinizing hormone (LH) surge triggers several tightly linked ovarian processes, including steroidogenesis, oocyte maturation, and ovulation. We designed studies to determine whether epidermal growth factor receptor (EGFR)-mediated signaling might serve as a common regulator of these activities. Our resu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamnongjit M,Gill A,Hammes SR

更新日期：2005-11-08 00:00:00

abstract：:Global warming is expected to lead to a large increase in atmospheric water vapor content and to changes in the hydrological cycle, which include an intensification of precipitation extremes. The intensity of precipitation extremes is widely held to increase proportionately to the increase in atmospheric water vapor c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Gorman PA,Schneider T

更新日期：2009-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences. In contrast, small interfering RNAs (siRNAs), which are derived by processing of long double-stranded RNAs and are o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng Y,Yi R,Cullen BR

更新日期：2003-08-19 00:00:00

abstract：:The decrease with age of the adrenal-secreted dehydroepiandrosterone sulfate (DHEAS) in serum has suggested that it may be causally related to longevity. For the PAQUID [People (Personnes) Aged (Agées) About What (Quid, in Latin)] cohort of elderly subjects, we have previously reported higher DHEAS in men than in wome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazat L,Lafont S,Berr C,Debuire B,Tessier JF,Dartigues JF,Baulieu EE

更新日期：2001-07-03 00:00:00

abstract：:Our group recently demonstrated that autoimmune T cells directed against central nervous system-associated myelin antigens protect neurons from secondary degeneration. We further showed that the synthetic peptide copolymer 1 (Cop-1), known to suppress experimental autoimmune encephalomyelitis, can be safely substitute...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schori H,Kipnis J,Yoles E,WoldeMussie E,Ruiz G,Wheeler LA,Schwartz M

更新日期：2001-03-13 00:00:00

abstract：:A stable complex between pentaammineruthenium(III) and histidine-33 in horse heart ferricytochrome c is formed in the reaction between aquopentaammineruthenium(II) and the protein at pH 7. HPLC of the tryptic hydrolysate of the modified protein was employed to identify the pentaammineruthenium binding site. Spectrosco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yocom KM,Shelton JB,Shelton JR,Schroeder WA,Worosila G,Isied SS,Bordignon E,Gray HB

更新日期：1982-11-01 00:00:00

abstract：:Maintaining active growth and effective immune responses is often costly for a living organism to survive. Fine-tuning the shared cross-regulators is crucial for metazoans and plants to make a trade-off between growth and immunity. The Arabidopsis regulatory receptor-like kinase BAK1 complexes with the receptor kinase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin W,Lu D,Gao X,Jiang S,Ma X,Wang Z,Mengiste T,He P,Shan L

更新日期：2013-07-16 00:00:00

abstract：:Activation of the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway is a frequent occurrence in human cancers and a major promoter of chemotherapeutic resistance. Inhibition of one downstream target in this pathway, mTORC1, has shown potential to improve chemosensitivity. However, the mechanisms and genetic m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mills JR,Hippo Y,Robert F,Chen SM,Malina A,Lin CJ,Trojahn U,Wendel HG,Charest A,Bronson RT,Kogan SC,Nadon R,Housman DE,Lowe SW,Pelletier J

更新日期：2008-08-05 00:00:00

abstract：:Chloroplast DNA (cpDNA) is under great photooxidative stress, yet its evolution is very conservative compared with nuclear or mitochondrial genomes. It can be expected that DNA repair mechanisms play important roles in cpDNA survival and evolution, but they are poorly understood. To gain insight into how the most seve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwon T,Huq E,Herrin DL

更新日期：2010-08-03 00:00:00

abstract：:Sodium/hydrogen exchangers (NHEs) are ubiquitous ion transporters that serve multiple cell functions. We have studied two mammalian isoforms, NHE1 (ubiquitous) and NHE3 (epithelial-specific), by measuring extracellular proton (H+) gradients during whole-cell patch clamp with perfusion of the cell interior. Maximal Na(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuster D,Moe OW,Hilgemann DW

更新日期：2004-07-13 00:00:00

abstract：:In t(15;17) acute promyelocytic leukemia, all-trans retinoic acid (RA) induces leukemic cell maturation in vitro and remission in acute promyelocytic leukemia patients, but in vivo treatments invariably lead to relapse with resistance to RA. NB4, a maturation-inducible cell line, and NB4-RAr sublines (R1 and R2) displ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruchaud S,Duprez E,Gendron MC,Houge G,Genieser HG,Jastorff B,Doskeland SO,Lanotte M

更新日期：1994-08-30 00:00:00

abstract：:This paper describes experiments in which cytosolic Ca2+ concentrations ([Ca2+]i) and catecholamine release were measured in two populations of chromaffin cells stimulated with a solution enriched in K+ (100 mM). Once depolarized, external Ca2+ or Ba2+ ions were offered to cells either as a single 2.5 mM step or as a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Michelena P,García-Pérez LE,Artalejo AR,García AG

更新日期：1993-04-15 00:00:00

abstract：:Sympathetic ganglia and some peripheral tissues of adult guinea pig and cat were analyzed by the indirect immunofluorescence technique with antisera to catecholamine-synthesizing enzymes and some peptides. In the guinea pig, noradrenergic neurons could be subdivided into three populations containing respectively (i) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lundberg JM,Hökfelt T,Anggård A,Terenius L,Elde R,Markey K,Goldstein M,Kimmel J

更新日期：1982-02-01 00:00:00

abstract：:Integration of viral DNA into the host nuclear genome, although not unusual in bacterial and animal systems, has surprisingly not been reported for plants. We have discovered geminvirus-related DNA (GRD) sequences, in the form of distinct sets of multiple direct repeats comprising three related repeat classes, situate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bejarano ER,Khashoggi A,Witty M,Lichtenstein C

更新日期：1996-01-23 00:00:00

abstract：:cDNA fragments of the homologues of the Drosophila head homeotic genes labial (lab), proboscipedia (pb), and Deformed (Dfd) have been isolated from the crustacean Porcellio scaber. Because the accumulation domains of the head homeotic complex (Hox) genes had not been previously reported for crustaceans, we studied the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abzhanov A,Kaufman TC

更新日期：1999-08-31 00:00:00

abstract：:Among the multiple steps constituting the kinesin mechanochemical cycle, one of the most interesting events is observed when kinesins move an 8-nm step from one microtubule (MT)-binding site to another. The stepping motion that occurs within a relatively short time scale ( approximately 100 mus) is, however, beyond th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyeon C,Onuchic JN

更新日期：2007-10-30 00:00:00

abstract：:Neurotrophins are essential for neuronal differentiation, but the onset and the intensity of neurotrophin signaling within the neuronal microenvironment are poorly understood. We tested the hypothesis that extracellular nucleotides and their cognate receptors regulate neurotrophin-mediated differentiation. We found th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arthur DB,Akassoglou K,Insel PA

更新日期：2005-12-27 00:00:00