Abstract：
:Despite the good clinical efficacy of interferon-alpha (IFNα) to treat some types of cancer and viral infections, this biological drug is underused given its severe adverse effects and high dosing parenteral regimens. Aiming to achieve a breakthrough in therapy with IFNα, this work reports for the first time on the design and full characterization of a novel nanomedicine of IFNα-2b-loaded chitosan nanoparticles (IFN-CT NPs) for oral delivery. IFN-CT NPs produced by ionotropic gelation, encapsulating approximately 100% of the drug, showed a size of 36 ± 8 nm, zeta potential of +30 mV (dynamic light scattering), and spherical morphology (transmission electron microscopy). The antiviral activity of IFN-CT NPs in vitro was comparable to that of commercial IFNα. Remarkably, both treatments stimulated the expression of IFN response genes to a similar extent in both noninfected and infected cells with Human Lymphotropic-T Virus type 1. Finally, oral administration of IFN-CT NPs (0.3 MIU) to CF1 mice showed detectable levels of IFNα in plasma after 1 h, whereas no IFNα was detected with a commercial formulation. These results are encouraging and open a new avenue for the administration of this biological drug in a minimally invasive, safer, and more patient-compliant way.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Cánepa C,Imperiale JC,Berini CA,Lewicki M,Sosnik A,Biglione MMdoi
10.1021/acs.biomac.7b00959subject
Has Abstractpub_date
2017-10-09 00:00:00pages
3302-3309issue
10eissn
1525-7797issn
1526-4602journal_volume
18pub_type
杂志文章abstract：:Ephemeral gels, called Enzgels, successively undergo sol-gel and then gel-sol transition under the action of two antagonistic enzymes, transglutaminase and protease. Molecular and macroscopic properties of Enzgels are directly dependent on the enzymatic activities and their ratios. This work studies the characteristic...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700601n
更新日期：2008-01-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701179s
更新日期：2008-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8007988
更新日期：2008-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060927c
更新日期：2007-02-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400680d
更新日期：2013-08-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01099
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0155221
更新日期：2001-10-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201143c
更新日期：2012-01-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1014365
更新日期：2011-04-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00108
更新日期：2018-04-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049295p
更新日期：2005-05-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00306
更新日期：2019-07-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01239
更新日期：2019-01-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0101318
更新日期：2001-01-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm010163v
更新日期：2002-05-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500750v
更新日期：2014-07-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301858a
更新日期：2013-02-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400290u
更新日期：2013-05-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401580k
更新日期：2014-01-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01333
更新日期：2019-01-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01211
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801123b
更新日期：2009-02-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050935d
更新日期：2006-04-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500168s
更新日期：2014-06-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050653d
更新日期：2005-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060671a
更新日期：2007-03-01 00:00:00
abstract：:N-Alkylated chitosan (NACS) may improve the blood clotting efficiency of chitosan (CS). To study its blood coagulation capability, a series of NACSs with various carbon chain lengths and degrees of substitution (DS) of alkyl groups were synthesized and characterized by FTIR, NMR, elemental analysis, and X-ray diffract...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01492
更新日期：2018-03-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00823
更新日期：2015-09-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500754r
更新日期：2014-08-11 00:00:00
abstract：:A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell line...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00776
更新日期：2016-10-10 00:00:00
