Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling.

Abstract:

:Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Kelly-Goss MR,Ning B,Bruce AC,Tavakol DN,Yi D,Hu S,Yates PA,Peirce SM

doi

10.1038/s41598-017-08982-z

subject

Has Abstract

pub_date

2017-08-22 00:00:00

pages

9049

issue

1

issn

2045-2322

pii

10.1038/s41598-017-08982-z

journal_volume

7

pub_type

杂志文章
  • Sub-lattice of Jahn-Teller centers in hexaferrite crystal.

    abstract::A novel type of sub-lattice of the Jahn-Teller (JT) centers was arranged in Ti-doped barium hexaferrite BaFe12O19. In the un-doped crystal all iron ions, sitting in five different crystallographic positions, are Fe3+ in the high-spin configuration (S = 5/2) and have a non-degenerate ground state. We show that the elec...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63915-7

    authors: Gudkov VV,Sarychev MN,Zherlitsyn S,Zhevstovskikh IV,Averkiev NS,Vinnik DA,Gudkova SA,Niewa R,Dressel M,Alyabyeva LN,Gorshunov BP,Bersuker IB

    更新日期:2020-04-27 00:00:00

  • Programmable hydraulic resistor for microfluidic chips using electrogate arrays.

    abstract::Flow rates play an important role in microfluidic devices because they affect the transport of chemicals and determine where and when (bio)chemical reactions occur in these devices. Flow rates can conveniently be determined using external peripherals in active microfluidics. However, setting specific flow rates in pas...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-53885-w

    authors: Salva ML,Temiz Y,Rocca M,Arango YC,Niemeyer CM,Delamarche E

    更新日期:2019-11-21 00:00:00

  • Combination of transcriptomic and metabolomic analyses reveals a JAZ repressor in the jasmonate signaling pathway of Salvia miltiorrhiza.

    abstract::Jasmonates (JAs) are plant-specific key signaling molecules that respond to various stimuli and are involved in the synthesis of secondary metabolites. However, little is known about the JA signal pathway, especially in economically significant medicinal plants. To determine the functions of novel genes that participa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14048

    authors: Ge Q,Zhang Y,Hua WP,Wu YC,Jin XX,Song SH,Wang ZZ

    更新日期:2015-09-21 00:00:00

  • Formulating formation mechanism of natural gas hydrates.

    abstract::A large amount of energy, perhaps twice the total amount of all other hydrocarbon reserves combined, is trapped within gas hydrate deposits. Despite emerging as a potential energy source for the world over the next several hundred years and one of the key factors in causing future climate change, gas hydrate is poorly...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06717-8

    authors: Palodkar AV,Jana AK

    更新日期:2017-07-25 00:00:00

  • Tuning bandgap and surface wettability of NiFe2O4 driven by phase transition.

    abstract::Stress variation induced bandgap tuning and surface wettability switching of spinel nickel ferrite (NiFe2O4, NFO) films were demonstrated and directly driven by phase transition via a post-annealing process. Firstly, the as-deposited NFO films showed hydrophilic surface with water contact angle (CA) value of 80 ± 1°. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-19319-9

    authors: Tong SK,Chi PW,Kung SH,Wei DH

    更新日期:2018-01-22 00:00:00

  • Multiplexed detection of RNA using MERFISH and branched DNA amplification.

    abstract::Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relatively modest brightness of signals from single RNA molecules can become ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43943-8

    authors: Xia C,Babcock HP,Moffitt JR,Zhuang X

    更新日期:2019-05-22 00:00:00

  • Fabrication of thickness controllable free-standing sandwich-structured hybrid carbon film for high-rate and high-power supercapacitor.

    abstract::Hybrid carbon films composed of graphene film and porous carbon film may give full play to the advantages of both carbon materials, and have great potential for application in energy storage and conversion devices. Unfortunately, there are very few reports on fabrication of hybrid carbon films. Here we demonstrate a s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07050

    authors: Wei H,Wei S,Tian W,Zhu D,Liu Y,Yuan L,Li X

    更新日期:2014-11-14 00:00:00

  • Publisher Correction: Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-24031-9

    authors: Sameni S,Malacrida L,Tan Z,Digman MA

    更新日期:2018-04-11 00:00:00

  • Thermal perturbation of NMR properties in small polar and non-polar molecules.

    abstract::Water is an important constituent in an abundant number of chemical systems; however, its presence complicates the analysis of in situ 1H MAS NMR investigations due to water's ease of solidification and vaporization, the large changes in mobility, affinity for hydrogen bonding interactions, etc., that are reflected by...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63174-6

    authors: Jaegers NR,Wang Y,Hu JZ

    更新日期:2020-04-08 00:00:00

  • A prospective, randomized, double-blind, placebo-controlled trial of acute postoperative pain treatment using opioid analgesics with intravenous ibuprofen after radical cervical cancer surgery.

    abstract::This study assessed the efficacy and tolerability of intravenous ibuprofen in the improvement of post-operative pain control and the reduction of opioid usage. Patients were randomly divided into placebo, ibuprofen 400 mg and ibuprofen 800 mg groups. All patients received patient-controlled intravenous morphine analge...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验

    doi:10.1038/s41598-018-28428-4

    authors: Liu X,Wang X,Zhao W,Wei L,Zhang P,Han F

    更新日期:2018-07-05 00:00:00

  • Intravoxel incoherent motion diffusion-weighted imaging to differentiate hepatocellular carcinoma from intrahepatic cholangiocarcinoma.

    abstract::The present study aimed to explore the value of intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) in differentiating hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma (ICC). This study included 65 patients with malignant hepatic nodules (55 with HCC, 10 with ICC), and 17 control pati...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-64804-9

    authors: Peng J,Zheng J,Yang C,Wang R,Zhou Y,Tao YY,Gong XQ,Wang WC,Zhang XM,Yang L

    更新日期:2020-05-07 00:00:00

  • NMR analysis reveals significant differences in the plasma metabolic profiles of Niemann Pick C1 patients, heterozygous carriers, and healthy controls.

    abstract::Niemann-Pick type C1 (NPC1) disease is a rare autosomal recessive, neurodegenerative lysosomal storage disorder, which presents with a range of clinical phenotypes and hence diagnosis remains a challenge. In view of these difficulties, the search for a novel, NPC1-specific biomarker (or set of biomarkers) is a topic o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06264-2

    authors: Probert F,Ruiz-Rodado V,Vruchte DT,Nicoli ER,Claridge TDW,Wassif CA,Farhat N,Porter FD,Platt FM,Grootveld M

    更新日期:2017-07-24 00:00:00

  • Three-dimensional ghost imaging lidar via sparsity constraint.

    abstract::Three-dimensional (3D) remote imaging attracts increasing attentions in capturing a target's characteristics. Although great progress for 3D remote imaging has been made with methods such as scanning imaging lidar and pulsed floodlight-illumination imaging lidar, either the detection range or application mode are limi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26133

    authors: Gong W,Zhao C,Yu H,Chen M,Xu W,Han S

    更新日期:2016-05-17 00:00:00

  • Activated Monocytes Enhance Platelet-Driven Contraction of Blood Clots via Tissue Factor Expression.

    abstract::Platelet-driven reduction in blood clot volume (clot contraction or retraction) has been implicated to play a role in hemostasis and thrombosis. Although these processes are often linked with inflammation, the role of inflammatory cells in contraction of blood clots and thrombi has not been investigated. The aim of th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05601-9

    authors: Peshkova AD,Le Minh G,Tutwiler V,Andrianova IA,Weisel JW,Litvinov RI

    更新日期:2017-07-11 00:00:00

  • Helicobacter pylori chemoreceptor TlpC mediates chemotaxis to lactate.

    abstract::It is recently appreciated that many bacterial chemoreceptors have ligand-binding domains (LBD) of the dCACHE family, a structure with two PAS-like subdomains, one membrane-proximal and the other membrane-distal. Previous studies had implicated only the membrane-distal subdomain in ligand recognition. Here, we report ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14372-2

    authors: Machuca MA,Johnson KS,Liu YC,Steer DL,Ottemann KM,Roujeinikova A

    更新日期:2017-10-26 00:00:00

  • Cytokines regulate complement receptor immunoglobulin expression and phagocytosis of Candida albicans in human macrophages: A control point in anti-microbial immunity.

    abstract::Complement Receptor Immunoglobulin (CRIg), selectively expressed by macrophages, plays an important role in innate immunity by promoting phagocytosis of bacteria. Thus modulation of CRIg on macrophages by cytokines can be an important mechanism by which cytokines regulate anti-microbial immunity. The effects of the cy...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-04325-0

    authors: Munawara U,Small AG,Quach A,Gorgani NN,Abbott CA,Ferrante A

    更新日期:2017-06-22 00:00:00

  • Increased H-Bond Stability Relates to Altered ε-Cleavage Efficiency and Aβ Levels in the I45T Familial Alzheimer's Disease Mutant of APP.

    abstract::Cleavage of the amyloid precursor protein's (APP) transmembrane domain (TMD) by γ-secretase is a crucial step in the aetiology of Alzheimer's Disease (AD). Mutations in the APP TMD alter cleavage and lead to familial forms of AD (FAD). The majority of FAD mutations shift the preference of initial cleavage from ε49 to ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41766-1

    authors: Götz A,Högel P,Silber M,Chaitoglou I,Luy B,Muhle-Goll C,Scharnagl C,Langosch D

    更新日期:2019-03-29 00:00:00

  • Freestanding photocatalytic materials based on 3D graphene and polyporphyrins.

    abstract::A new concept in the formulation of hybrid nanostructured materials combining high quality graphene 3D supported by Nickel foam and polyporphyrins for visible light photocatalytic application is here reported. Our innovative approach involves the development of a freestanding device able to: i) offer a high surface ar...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23345-y

    authors: Ussia M,Bruno E,Spina E,Vitalini D,Pellegrino G,Ruffino F,Privitera V,Carroccio SC

    更新日期:2018-03-22 00:00:00

  • Aggregation affects optical properties and photothermal heating of gold nanospheres.

    abstract::Laser heating of gold nanospheres (GNS) is increasingly prevalent in biomedical applications due to tunable optical properties that determine heating efficiency. Although many geometric parameters (i.e. size, morphology) can affect optical properties of individual GNS and their heating, no specific studies of how GNS ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79393-w

    authors: Wang Y,Gao Z,Han Z,Liu Y,Yang H,Akkin T,Hogan CJ,Bischof JC

    更新日期:2021-01-13 00:00:00

  • Highly variable COI haplotype diversity between three species of invasive pest fruit fly reflects remarkably incongruent demographic histories.

    abstract::Distance decay principles predict that species with larger geographic ranges would have greater intraspecific genetic diversity than more restricted species. However, invasive pest species may not follow this prediction, with confounding implications for tracking phenomena including original ranges, invasion pathways ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63973-x

    authors: Doorenweerd C,San Jose M,Barr N,Leblanc L,Rubinoff D

    更新日期:2020-04-23 00:00:00

  • Improving orthotopic mouse models of patient-derived breast cancer brain metastases by a modified intracarotid injection method.

    abstract::Breast cancer brain metastasis (BCBM) remains a major clinical problem. Approximately 10-16% of patients with breast cancer develop brain metastases (BCBM). However, no systemic therapy has gained regulatory approval for the specific treatment of BCBM and this remains an area of persistent, unmet medical need. Rapid, ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36874-3

    authors: Liu Z,Wang Y,Kabraji S,Xie S,Pan P,Liu Z,Ni J,Zhao JJ

    更新日期:2019-01-24 00:00:00

  • Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo.

    abstract::Tail-anchored (TA) proteins are post-translationally inserted into membranes. The TRC40 pathway targets TA proteins to the endoplasmic reticulum via a receptor comprised of WRB and CAML. TRC40 pathway clients have been identified using in vitro assays, however, the relevance of the TRC40 pathway in vivo remains unknow...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39464

    authors: Rivera-Monroy J,Musiol L,Unthan-Fechner K,Farkas Á,Clancy A,Coy-Vergara J,Weill U,Gockel S,Lin SY,Corey DP,Kohl T,Ströbel P,Schuldiner M,Schwappach B,Vilardi F

    更新日期:2016-12-21 00:00:00

  • 2H-NbS2 film as a novel counter electrode for meso-structured perovskite solar cells.

    abstract::We report the use of 2H-NbS2 film as a novel counter electrode in perovskite solar cells fabricated with a cold isostatic pressing method. The 2H-NbS2 film, which was prepared through an exfoliation method followed by restacking from LixNbS2 powder, shows high electrical conductivity of 8.7 × 103 S cm-1 and work funct...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-25449-x

    authors: Shao F,Tian Z,Qin P,Bu K,Zhao W,Xu L,Wang D,Huang F

    更新日期:2018-05-04 00:00:00

  • Self-biased 215 MHz magnetoelectric NEMS resonator for ultra-sensitive DC magnetic field detection.

    abstract::High sensitivity magnetoelectric sensors with their electromechanical resonance frequencies < 200 kHz have been recently demonstrated using magnetostrictive/piezoelectric magnetoelectric heterostructures. In this work, we demonstrate a novel magnetoelectric nano-electromechanical systems (NEMS) resonator with an elect...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01985

    authors: Nan T,Hui Y,Rinaldi M,Sun NX

    更新日期:2013-01-01 00:00:00

  • Stable isotopes of carbon and nitrogen help to predict the belowground communities at a regional scale.

    abstract::At the regional scale, although environmental factors are known to shape the distributions of belowground communities in terrestrial ecosystems, these environmental factors account for relatively low percentages of the variation in belowground communities. More of this variation might be explained by considering ecosy...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07517-w

    authors: Wang B,Wu Y,Chen D

    更新日期:2017-08-04 00:00:00

  • Origin of the improved mobility and photo-bias stability in a double-channel metal oxide transistor.

    abstract::This study examined the performance and photo-bias stability of double-channel ZnSnO/InZnO (ZTO/IZO) thin-film transistors. The field-effect mobility (μFE) and photo-bias stability of the double-channel device were improved by increasing the thickness of the front IZO film (t(int)) compared to the single-ZTO-channel d...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03765

    authors: Jung HY,Kang Y,Hwang AY,Lee CK,Han S,Kim DH,Bae JU,Shin WS,Jeong JK

    更新日期:2014-01-20 00:00:00

  • A novel approach for discovering stochastic models behind data applied to El Niño-Southern Oscillation.

    abstract::Stochastic differential equations (SDEs) are ubiquitous across disciplines, and uncovering SDEs driving observed time series data is a key scientific challenge. Most previous work on this topic has relied on restrictive assumptions, undermining the generality of these approaches. We present a novel technique to uncove...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81162-2

    authors: Olson R,An SI,Kim SK,Fan Y

    更新日期:2021-01-29 00:00:00

  • A direct comparison of interphase FISH versus low-coverage single cell sequencing to detect aneuploidy reveals respective strengths and weaknesses.

    abstract::Aneuploidy has been reported to occur at remarkably high levels in normal somatic tissues using Fluorescence In Situ Hybridization (FISH). Recently, these reports were contradicted by single-cell low-coverage whole genome sequencing (scL-WGS) analyses, which showed aneuploidy frequencies at least an order of magnitude...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46606-w

    authors: Andriani GA,Maggi E,Piqué D,Zimmerman SE,Lee M,Quispe-Tintaya W,Maslov A,Campisi J,Vijg J,Mar JC,Montagna C

    更新日期:2019-07-19 00:00:00

  • Rhythmic synchronization and hybrid collective states of globally coupled oscillators.

    abstract::Macroscopic rhythms are often signatures of healthy functioning in living organisms, but they are still poorly understood on their microscopic bases. Globally interacting oscillators with heterogeneous couplings are here considered. Thorough theoretical and numerical analyses indicate the presence of multiple phase tr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31278-9

    authors: Qiu T,Bonamassa I,Boccaletti S,Liu Z,Guan S

    更新日期:2018-08-28 00:00:00

  • Detection and segmentation of cell nuclei in virtual microscopy images: a minimum-model approach.

    abstract::Automated image analysis of cells and tissues has been an active research field in medical informatics for decades but has recently attracted increased attention due to developments in computer and microscopy hardware and the awareness that scientific and diagnostic pathology require novel approaches to perform object...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00503

    authors: Wienert S,Heim D,Saeger K,Stenzinger A,Beil M,Hufnagl P,Dietel M,Denkert C,Klauschen F

    更新日期:2012-01-01 00:00:00