Abstract：
:The objective of this study was to investigate the molecular mechanisms explaining the multidrug-resistant (MDR) phenotype found in a novel clinical Shewanella sp. strain (Shew256) recovered from a diabetic patient. Whole-genome shotgun sequencing was performed using Illumina MiSeq-I and Nextera XT DNA library. De novo assembly was performed with SPAdes. RAST Server was used to predict the open-reading frames and the predictions were confirmed using BLAST. Further genomic analysis was carried out using average nucleotide identity (ANI), ACT (Artemis), OrthoMCL, ARG-ANNOT, ISfinder, PHAST, tRNAscan-SE, plasmidSPAdes, PlasmidFinder and MAUVE. PCR and plasmid extraction were also performed. Genomic analysis revealed a total of 456 predicted genes unique to Shew256 compared with other Shewanella genomes. Moreover, the presence of different resistance genes, including blaPER-2, was found. A complex class 1 integron containing the ISCR1 gene, disrupted by two putative transposase genes, was identified. Furthermore, other resistance genes, a transposon containing aph(3'), insertion sequences, phages and non-coding RNAs were also found. In conclusion, evidence of acquisition of resistance genes and mobile elements that could explain the MDR phenotype were observed. This Shewanella sp. represents a prime example of how antibiotic resistance determinants can be acquired by uncommon pathogens.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Almuzara M,Montaña S,Lazzaro T,Uong S,Parmeciano Di Noto G,Traglia G,Bakai R,Centrón D,Iriarte A,Quiroga C,Ramirez MSdoi
10.1016/j.jgar.2017.06.005subject
Has Abstractpub_date
2017-12-01 00:00:00pages
81-86eissn
2213-7165issn
2213-7173pii
S2213-7165(17)30117-0journal_volume
11pub_type
杂志文章abstract：OBJECTIVES:Marine bivalves can act as bioindicators of marine environment pollution by multidrug-resistant (MDR) enteric bacteria of medical interest. The aim of this study was to report the draft genome sequence of a plasmid-encoded AmpC (pAmpC) (CMY-2)-carrying Escherichia coli isolate recovered from a marine bivalve...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.06.010
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. The present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important Saudi scorpions (Androcton...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.03.008
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Ralstonia pickettii has been increasingly recognised as an emerging opportunistic pathogen in hospital settings in recent years, especially in patients with prolonged hospital stay. Clinical manifestations associated with R. pickettii infection range from mild infections to severe invasive life-threatening i...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.02.020
更新日期：2020-06-01 00:00:00
abstract：:Acinetobacter calcoaceticus-baumannii complex is a common cause of hospital-acquired infections (HAIs) globally, remarkable for its high rate of antibiotic resistance, including to carbapenems. There are few data on the resistance of A. baumannii in Vietnam, which are essential for developing evidence-based treatment ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2014.05.003
更新日期：2014-12-01 00:00:00
abstract：BACKGROUND AND AIM:The global rise of antimicrobial resistance among bacterial strains is a rapidly growing challenge and is becoming a major public health concern. This study documents the worldwide spread and genotype distribution of human clinical isolates of New Delhi metallo-β-lactamase-producing Klebsiella pneumo...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2020.10.016
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:Escherichia coli and Enterobacter cloacae are two major pathogens causing urinary tract infection (UTI). Here we characterised the genome of an NDM-1-producing E. cloacae strain and an extended-spectrum β-lactamase (ESBL)-producing E. coli strain that were co-isolated from a UTI. METHODS:The genomes of E. c...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.04.007
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr gene in Pasteurella multocida of avian origin from China. METHODS:A total of 113 P. multocida isolates were collected from sick poultries (ducks, chickens and geese) from 2003 to 2016 in Southern Ch...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.09.018
更新日期：2020-12-01 00:00:00
abstract：:This study aimed to evaluate the clinical outcomes of vancomycin-resistant enterococcal bloodstream infections (VRE BSI) caused by Enterococcus gallinarum or Enterococcus casseliflavus. Variables associated with treatment failure were determined and treatment options were compared. This was a national retrospective st...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2015.12.002
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total o...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.09.017
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:The production of β-lactamase enzymes such as AmpC β-lactamases and extended-spectrum β-lactamases (ESBLs) is among the main mechanisms for resistance to expanded-spectrum cephalosporins. The present study was conducted to investigate the prevalence and molecular epidemiology of plasmid-mediated AmpC beta (β)...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.03.011
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:Recently, the virulence factor sasX was described on the mobile genetic element φSPβ prophage in a ST239-SCCmecIII methicillin-resistant Staphylococcus aureus (MRSA) clone. The aim of this study was to identify sesI, an sasX homologue, in an MRSA ST239 strain. METHODS:MRSA strain VB1490 was isolated from a ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.02.006
更新日期：2019-09-01 00:00:00
abstract：:According to the World Health Organization (WHO), as of today, there are 2.165.500 confirmed cases of the novel coronavirus disease (COVID-19) and 145.705 deaths in over 185 countries. Unfortunately, despite the tremendous efforts to develop a vaccine initiated by various leading health institutions all over the world...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.07.005
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:Excessive use of antimicrobial agents for acute upper respiratory infections (URIs) is one of the most common problems in clinical practice. Interventions are required to reduce antimicrobial overuse in these common infections. METHODS:During a 9-month study period (June 2014-February 2015), a quasi-experim...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.08.015
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:Multidrug-resistant bacteria have become a serious threat worldwide. In particular, the coexistence of carbapenemase genes and mcr-1 leaves few available treatment options. Here we report a multidrug-resistant Escherichia coli isolate harbouring both mcr-1 and blaNDM-9 from a patient with a urinary tract inf...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.10.003
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:There are numerous reported cases of extended-spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae in Nigeria. Yet, there are regions in Nigeria where cases of ESBL were under-reported. The review aims to assess the prevalence of ESBL-producing Gram-negative bacteria in Nigeria and the genes involved...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2020.04.010
更新日期：2020-09-01 00:00:00
abstract：:Enterococcus faecium was considered an important nosocomial pathogen that could incur a substantial health burden owing to its high drug resistance and its ability to adapt to the hospital niche. Here we presented the draft genome sequence of multidrug-resistant E. faecium XH877. The draft genome sequence was composed...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.07.004
更新日期：2016-12-01 00:00:00
abstract：:Uropathogenic Escherichia coli (UPEC) is the most frequent cause of urinary tract infection (UTI). Virulence factors (VFs) of UPEC in children are not well known. Circulating antibiotic resistance mechanisms in the community are increasing. In this study, the aetiological agents of UTI and antibiotic resistance mechan...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2014.08.003
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVES:Pseudomonas aeruginosa is the most frequent infectious agent in cystic fibrosis patients. P. aeruginosa resistance to first line antibiotics limits therapeutic options, but the therapeutic potential of older generation antibiotics, such as fosfomycin is under investigation. Fosfomycin does not belong to any ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.02.014
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVES:Delafloxacin is a broad-spectrum anionic fluoroquinolone with activity against Gram-positive and Gram-negative organisms, including methicillin-resistant Staphylococcus aureus. Both oral and intravenous formulations were approved for use in the treatment of acute bacterial skin and skin-structure infections ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.09.027
更新日期：2020-12-01 00:00:00
abstract：:Various molecular methods have been developed to rapidly detect clarithromycin (CLR) resistance in Helicobacter pylori isolates in clinical specimens. All of these assays for detecting CLR resistance in H. pylori are based on detection of mutations in the 23S rRNA gene. In this article, we summarise current knowledge ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2016.01.002
更新日期：2016-03-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to examine the performance of the chromogenic β LACTA™ test for the rapid detection of expanded-spectrum cephalosporin (ESC) non-susceptibility among Enterobacteriaceae in a region endemic for potent β-lactamases. METHODS:The β LACTA™ test was applied prospectively on 235 consecuti...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.05.018
更新日期：2017-09-01 00:00:00
abstract：:Environmental fungicides are used in agriculture to reduce fungal spoilage of crops to a minimum, and the polyene macrolide natamycin is used as a food preservative. The use of natamycin in yoghurt has recently been authorised in the USA and some other countries. However, resistance development is a serious risk assoc...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2017.10.024
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVES:Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities. The aim of this study was to analyse the resistance, virulence, and epidemiological and genetic relationships of S. aureus from bloodstream infections (BSIs) and pneumonia from patients in Souther...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.07.009
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVES:Treatment of leishmaniasis remains a challenge, especially due to the need for multiple painful injections, the toxicity of current drugs against the disease, their lack of efficacy and, lately, drug resistance. The aim of this study was to demonstrate the biological behaviour of 3-nitro-2'-hydroxy-4',6'-dim...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.11.012
更新日期：2018-06-01 00:00:00
abstract：:β-Lactams are a commonly used class of bactericidal antibiotics. The number of β-lactam-resistant pathogens is constantly increasing in hospitals around the world. Interestingly, most of the β-lactam-resistant bacteria carry mobile genetic elements, such as conjugative plasmids, that render the pathogen resistant. The...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2014.02.005
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVES:Rates of nosocomial infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) have increased. A meta-analysis was conducted to explore risk factors for CRKP infection in order to provide a theoretical basis for reducing the CRKP infection rate and actively preventing CRKP infection. METHODS:Onl...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.09.006
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVES:With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.12.015
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVES:Acinetobacter baumannii has emerged as a prevalent multidrug-resistant nosocomial pathogen worldwide. Here we report the draft genome sequence of A. baumannii strain Ab174 isolated from a neonatal patient diagnosed with acute peritonitis. METHODS:The draft genome sequence of A. baumannii Ab174 was determine...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.06.021
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVES:Farm animals have been recognised as important carriers and reservoirs of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The aim of this study was to report the draft genome sequences of two multidrug-resistant (MDR) CTX-M-15-producing E. coli strains (47VL and 13B) isolated from different ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.10.009
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVE:Little is known about the treatment outcomes of HIV-negative drug-resistant tuberculosis (TB) patients in Malaysia. With respect to this issue, this study aimed to determine factors associated with unsuccessful treatment outcomes among drug-resistant TB patients at the Institute of Respiratory Medicine, Kuala...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.05.009
更新日期：2019-12-01 00:00:00
