Evaluation of the in vitro antimicrobial activity of selected Saudi scorpion venoms tested against multidrug-resistant micro-organisms.

Abstract:

OBJECTIVES:Scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. The present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important Saudi scorpions (Androctonus crassicauda, Androctonus bicolor and Leiurus quinquestriatus). METHODS:Antimicrobial assays were performed using a microplate growth inhibition assay against 10 multidrug-resistant (MDR) micro-organisms (4 Gram-negative bacteria, 2 Gram-positive bacteria and 4 fungi and yeasts) at concentrations ranging from 0 to 20mg/mL of each venom. Following qualitative analysis, dose-response assays were performed for bacterial and fungal killing curves using the MTT colorimetric assay. RESULTS:Among the three tested scorpion venoms, only L. quinquestriatus venom showed significant broad-spectrum antimicrobial activity in a dose-dependent manner from 5 to 20mg/mL. Leiurus quinquestriatus venom inhibited the growth and survival of MDR Escherichia coli (55.2%), Acinetobacter baumannii (50.6%), Klebsiella pneumoniae (35.1%), Pseudomonas aeruginosa (31.3%), Staphylococcus aureus (36.4%), Enterococcus faecalis (47.6%), Candida albicans (31.2%) and Candida glabrata (39.0%), whereas no significant activity against Fusarium oxysporum and Aspergillus flavus was observed. In contrast, the venoms of A. crassicauda and A. bicolor did not show noticeable antimicrobial activity against any of the tested organisms. CONCLUSIONS:The findings of the current study demonstrate that L. quinquestriatus venom possesses antimicrobial activity and thus can be used as a template for designing and development of novel antimicrobial drugs.

authors

Al-Asmari AK,Alamri MA,Almasoudi AS,Abbasmanthiri R,Mahfoud M

doi

10.1016/j.jgar.2017.03.008

subject

Has Abstract

pub_date

2017-09-01 00:00:00

pages

14-18

eissn

2213-7165

issn

2213-7173

pii

S2213-7165(17)30073-5

journal_volume

10

pub_type

杂志文章
  • Investigation of pre-clinical pharmacokinetic parameters of atovaquone nanosuspension prepared using a pH-based precipitation method and its pharmacodynamic properties in a novel artemisinin combination.

    abstract:OBJECTIVES:Recently, a growing resistance to antimalarial drugs such as chloroquine, sulfadoxine-pyrimethamine, artemisinin derivatives and mefloquine has been observed. The pharmacokinetic limitation of the current therapy and multi-drug resistance has resulted in an urgent need to study the new antimalarial combinati...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.02.018

    authors: Kathpalia H,Juvekar S,Mohanraj K,Apsingekar M,Shidhaye S

    更新日期:2020-09-01 00:00:00

  • Antimicrobial activity of LysSS, a novel phage endolysin, against Acinetobacter baumannii and Pseudomonas aeruginosa.

    abstract:OBJECTIVES:Multidrug-resistant (MDR) bacteria are a major public-health concern. Bacteriophage endolysins (lysins) can be used as novel antimicrobial agents against bacterial infections. In this study, a novel endolysin (LysSS) containing a lysozyme-like domain was evaluated for its antibacterial activity against vario...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.01.005

    authors: Kim S,Lee DW,Jin JS,Kim J

    更新日期:2020-09-01 00:00:00

  • Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil.

    abstract::The aim of this work was to analyse extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from outpatients with signs of cystitis in Hospital Universitário de Brasília (Brasília, Brazil) during the period July 2013 to April 2014. E. coli isolated from urine culture were identified and their ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.02.003

    authors: Gonçalves LF,de Oliveira Martins-Júnior P,de Melo ABF,da Silva RCRM,de Paulo Martins V,Pitondo-Silva A,de Campos TA

    更新日期:2016-09-01 00:00:00

  • Antimicrobial stewardship in hospitals: Does it work and can we do it?

    abstract::Selection of resistant pathogens by antimicrobial use is probably the most important cause of antimicrobial resistance. Antimicrobial stewardship (AMS) refers to a multifaceted approach to optimise prescribing. The benefits of AMS programmes have been widely demonstrated in terms of reductions in antimicrobial use, mo...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2013.08.001

    authors: Levy Hara G

    更新日期:2014-03-01 00:00:00

  • Rectal screening displays high negative predictive value for bloodstream infection with (ESBL-producing) Gram-negative bacteria in neonates with suspected sepsis in a low-resource setting neonatal care unit.

    abstract:OBJECTIVES:We analysed the concordance of rectal swab isolates and blood culture for Gram-negative bacteria (GNB) isolates in neonates with a suspicion of neonatal sepsis admitted to a neonatal care unit in Haiti. METHODS:We matched pairs of blood and rectal samples taken on the date of suspected sepsis onset in the s...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.08.017

    authors: Lenglet A,Schuurmans J,Ariti C,Borgundvaag E,Charles K,Badjo C,Clezy K,Evens E,Senat-Delva R,Berthet M,Lekkerkerker M,McRae M,Wertheim H,Hopman J

    更新日期:2020-12-01 00:00:00

  • Genetic characterisation of a whiB7 mutant of a Mycobacterium tuberculosis clinical strain.

    abstract::Mycobacterium tuberculosis is naturally resistant to clarithromycin (CLR). The genes Rv3197A (whiB7) and Rv1988 (ermMT) have been shown to be involved in the resistant phenotype. In this study, a CLR-susceptible M. tuberculosis clinical strain was identified, designated as DS3214, and the nucleotide sequences and expr...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2015.07.004

    authors: Warit S,Phunpruch S,Jityam C,Jaitrong S,Billamas P,Chaiprasert A,Palittapongarnpim P,Prammananan T

    更新日期:2015-12-01 00:00:00

  • A new effective antiplasmodial compound: Nanoformulated pyrimethamine.

    abstract:OBJECTIVES:The aim of this study was to evaluate the efficacy of pyrimethamine-loaded poloxamer 407 nanomicelles on Plasmodium berghei strain NICD in vivo. METHODS:Pyrimethamine-loaded nanomicelles were prepared and their zeta potential, particle size and polydispersity index were measured. For antiplasmodial assessme...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.08.002

    authors: Pestehchian N,Vafaei MR,Nematolahy P,Varshosaz J,Yousefi HA,Bide VZ,Kalani H

    更新日期:2020-03-01 00:00:00

  • Infections by OXA-48-like-producing Klebsiella pneumoniae non-co-producing extended-spectrum beta-lactamase: Can they be successfully treated with cephalosporins?

    abstract:BACKGROUND:OXA-48 is an Ambler class D β-lactamase that hydrolyses penicillin and imipenem but has poor hydrolytic activity against cephalosporins. However, very few clinical experiences of treating extended-spectrum β-lactamase (ESBL)-negative OXA-48 producers with cephalosporins have been published. OBJECTIVES:The a...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.02.016

    authors: Escolà-Vergé L,Larrosa N,Los-Arcos I,Viñado B,González-López JJ,Pigrau C,Almirante B,Len O

    更新日期:2019-12-01 00:00:00

  • Genomic analysis of two bacterial strains co-isolated from a urinary tract infection: NDM-1-producing Enterobacter cloacae accompanied by extended-spectrum β-lactamase-producing Escherichia coli.

    abstract:OBJECTIVES:Escherichia coli and Enterobacter cloacae are two major pathogens causing urinary tract infection (UTI). Here we characterised the genome of an NDM-1-producing E. cloacae strain and an extended-spectrum β-lactamase (ESBL)-producing E. coli strain that were co-isolated from a UTI. METHODS:The genomes of E. c...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.04.007

    authors: Xu J,He F

    更新日期:2019-06-01 00:00:00

  • Urinary tract infection in Uruguayan children: Aetiology, antimicrobial resistance and uropathogenic Escherichia coli virulotyping.

    abstract::Uropathogenic Escherichia coli (UPEC) is the most frequent cause of urinary tract infection (UTI). Virulence factors (VFs) of UPEC in children are not well known. Circulating antibiotic resistance mechanisms in the community are increasing. In this study, the aetiological agents of UTI and antibiotic resistance mechan...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2014.08.003

    authors: Robino L,García-Fulgueiras V,Araujo L,Algorta G,Pírez MC,Vignoli R

    更新日期:2014-12-01 00:00:00

  • High rates of CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae in wild boars and Barbary macaques in Algeria.

    abstract:OBJECTIVES:The present study aimed to screen for the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in wild boars and Barbary macaques in Béjaïa and Jijel, Algeria. METHODS:A total of 216 faecal samples collected between September 2014 and August 2015 were cultured on MacConkey agar supp...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.10.005

    authors: Bachiri T,Bakour S,Ladjouzi R,Thongpan L,Rolain JM,Touati A

    更新日期:2017-03-01 00:00:00

  • Epidemiology of multidrug-resistant Gram-negative pathogens isolated from ventilator-associated pneumonia in ICU patients.

    abstract:OBJECTIVES:Antibiotic resistance among Gram-negative pathogens isolated from ventilator-associated pneumonia (VAP) poses a grave threat in intensive care unit (ICU) patients. The aim of this study was to assess the prevalence of pathogens in ICU patients and their drug resistance profile. The prevalence of extended-spe...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.12.016

    authors: Gupta R,Malik A,Rizvi M,Ahmed M,Singh A

    更新日期:2017-06-01 00:00:00

  • High frequency of aac(6')-Ib-cr gene associated with double mutations in gyrA and parC in Escherichia coli isolates from patients with urinary tract infections.

    abstract:OBJECTIVES:The aims of this study were (i) to determine the frequency of plasmid-mediated resistance to fluoroquinolones (FQs) in Escherichia coli isolated from patients with urinary tract infections (UTIs) of nosocomial and community origin and (ii) to determine the relationships between the presence of extended-spect...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.12.013

    authors: Volcão LM,Lacava JP,Gewehr MF,Leal VL,Ramis IB,Ramos DF,Gonçalves CV,Possuelo LG,Minarini LAR,da Silva PEA,von Groll A

    更新日期:2018-06-01 00:00:00

  • Evaluation of vancomycin MIC creep in Staphylococcus aureus.

    abstract:OBJECTIVES:Vancomycin is the primary treatment for methicillin-resistant Staphylococcus aureus (MRSA). However, an increasing proportion of MRSA isolates with high minimum inhibitory concentrations (MICs) within the susceptible range (vancomycin 'MIC creep') is being observed. The aim of this study was to assess the va...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.04.007

    authors: Diaz R,Ramalheira E,Afreixo V,Gago B

    更新日期:2017-09-01 00:00:00

  • Draft genome sequence of a blaCMY-2/IncI1-harbouring Escherichia coli D:ST457 isolated from coastal benthic organisms.

    abstract:OBJECTIVES:Marine bivalves can act as bioindicators of marine environment pollution by multidrug-resistant (MDR) enteric bacteria of medical interest. The aim of this study was to report the draft genome sequence of a plasmid-encoded AmpC (pAmpC) (CMY-2)-carrying Escherichia coli isolate recovered from a marine bivalve...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.06.010

    authors: Sellera FP,Fernandes MR,Moura Q,Lopes RB,Souza TA,Cerdeira L,Lincopan N

    更新日期:2018-09-01 00:00:00

  • Antibiotic use in food animals worldwide, with a focus on Africa: Pluses and minuses.

    abstract::Antibiotics are frequently used in food animal production in developing countries to promote the well-being and growth of animals. This practice provides some economic benefits to producers and consumers at large. Nevertheless, this practice is also associated with a number of concerns. A major concern has been that r...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2019.07.031

    authors: Van TTH,Yidana Z,Smooker PM,Coloe PJ

    更新日期:2020-03-01 00:00:00

  • Draft genome sequences of two fluoroquinolone-resistant CTX-M-15-producing Escherichia coli ST90 (ST23 complex) isolated from a calf and a dairy cow in South America.

    abstract:OBJECTIVES:Farm animals have been recognised as important carriers and reservoirs of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The aim of this study was to report the draft genome sequences of two multidrug-resistant (MDR) CTX-M-15-producing E. coli strains (47VL and 13B) isolated from different ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.10.009

    authors: Sartori L,Fernandes MR,Ienne S,de Souza TA,Gregory L,Cerdeira L,Lincopan N

    更新日期:2017-12-01 00:00:00

  • Complete genome arrangement revealed the emergence of a poultry origin superbug Citrobacter portucalensis strain NR-12.

    abstract:OBJECTIVES:Citrobacter spp. are part of normal human and animal intestinal flora. Citrobacter portucalensis (C. portucalensis) is closely related to Citrobacter freundii, which is an emerging opportunistic nosocomial pathogen. The aim of this study was to retrieve colistin-resistant Citrobacter spp. from poultry in Ban...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.05.031

    authors: Hasan MS,Sultana M,Hossain MA

    更新日期:2019-09-01 00:00:00

  • Plasmid metagenomics reveals multiple antibiotic resistance gene classes among the gut microbiomes of hospitalised patients.

    abstract::Antibiotic resistance genes are rapidly spread between pathogens and the normal flora, with plasmids playing an important role in their circulation. This study aimed to investigate antibiotic resistance plasmids in the gut microbiome of hospitalised patients. Stool samples were collected from seven inpatients at Sirir...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.03.001

    authors: Jitwasinkul T,Suriyaphol P,Tangphatsornruang S,Hansen MA,Hansen LH,Sørensen SJ,Permpikul C,Rongrungruang Y,Tribuddharat C

    更新日期:2016-09-01 00:00:00

  • IncC blaKPC-2-positive plasmid characterised from ST648 Escherichia coli.

    abstract:OBJECTIVES:This study describes the characterisation of type 2 IncC plasmids pC-Ec20-KPC and pC-Ec2-KPC, carrying theblaKPC-2 gene, from two multiresistant Escherichia coli recovered in University Hospital of Larissa (Greece) in 2018. METHODS:E. coli strains Ec-2Lar and Ec-20Lar were recovered from rectal swabs of two...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.05.001

    authors: Papagiannitsis CC,Bitar I,Malli E,Tsilipounidaki K,Hrabak J,Petinaki E

    更新日期:2019-12-01 00:00:00

  • Biofilm formation and transfer of a streptomycin resistance gene in enterococci from fermented pork.

    abstract:OBJECTIVES:Multidrug-resistant (MDR) enterococci are found extensively in food samples. This study characterized the phenotypic virulence factors and the ability of horizontal gene transfer of a streptomycin resistance gene among enterococci isolated from fermented pork. METHODS:Thirty-six MDR enterococci were subject...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.04.016

    authors: Chotinantakul K,Chansiw N,Okada S

    更新日期:2020-09-01 00:00:00

  • Genotypic analysis of uropathogenic Escherichia coli to understand factors that impact the prevalence of β-lactam-resistant urinary tract infections in a community.

    abstract:OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.03.002

    authors: Tarlton NJ,Moritz C,Adams-Sapper S,Riley LW

    更新日期:2019-12-01 00:00:00

  • Genotypic and phylogenetic characterisation of a clinical Ralstonia pickettii strain carrying two novel OXA allelic variants, blaOXA-898 and blaOXA-899, isolated from a bloodstream infection in China.

    abstract:OBJECTIVES:Ralstonia pickettii has been increasingly recognised as an emerging opportunistic pathogen in hospital settings in recent years, especially in patients with prolonged hospital stay. Clinical manifestations associated with R. pickettii infection range from mild infections to severe invasive life-threatening i...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.02.020

    authors: Jiang T,Xu J,He F

    更新日期:2020-06-01 00:00:00

  • Clinical epidemiology of vancomycin-resistant Enterococcus gallinarum and Enterococcus casseliflavus bloodstream infections.

    abstract::This study aimed to evaluate the clinical outcomes of vancomycin-resistant enterococcal bloodstream infections (VRE BSI) caused by Enterococcus gallinarum or Enterococcus casseliflavus. Variables associated with treatment failure were determined and treatment options were compared. This was a national retrospective st...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2015.12.002

    authors: Britt NS,Potter EM

    更新日期:2016-06-01 00:00:00

  • Manganese complex [Mn(CO)3(tpa-κ3N)]Br increases antibiotic sensitivity in multidrug resistant Streptococcus pneumoniae.

    abstract:OBJECTIVES:The emergence of multidrug-resistance (MDR) in Streptococcus pneumoniae clones and non-vaccine serotypes necessitate the development of novel treatment strategies. This work aimed to determine the efficacy of the Mn complex [Mn(CO)3(tpa-κ3N)]Br against clinically important MDR strains of S. pneumoniae. METH...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.04.031

    authors: Liakopoulos A,La Ragione RM,Nagel C,Schatzschneider U,Rozen DE,Betts JW

    更新日期:2020-09-01 00:00:00

  • Pharmacokinetics of meropenem in burn patients with infections caused by Gram-negative bacteria: Are we getting close to the right treatment?

    abstract:OBJECTIVES:Infections caused by multidrug-resistant Gram-negative bacteria are associated with high mortality. A relevant concern is the efficacy of antibiotic therapy in burn patients in whom pathophysiological changes strongly influence pharmacokinetic (PK) parameters. This study aimed to describe the PK parameters o...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.06.011

    authors: Corcione S,D'Avolio A,Loia RC,Pensa A,Segala FV,De Nicolò A,Fatiguso G,Romeo M,Di Perri G,Stella M,De Rosa FG

    更新日期:2020-03-01 00:00:00

  • Aminoquinolines afford resistance to cerebral malaria in susceptible mice.

    abstract:OBJECTIVES:Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. METHODS:In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falci...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.07.027

    authors: Srbljanović J,Bobić B,Štajner T,Uzelac A,Opsenica I,Terzić-Jovanović N,Bauman N,Šolaja BA,Djurković-Djaković O

    更新日期:2020-12-01 00:00:00

  • Comparative study of the impact of the administration of Amoxicillin and Algo-Bio® alternative substance to antibiotics, on the level of selection of resistant Enterobacteriaceae in the digestive flora of piglets.

    abstract:OBJECTIVES:The aim of study was to evaluate by comparative study the level of selection of antibiotic-resistant Enterobacteriaceae in the digestive microbiota of piglets when using amoxicillin and Algo-Bio®. METHODS:Amoxicillin and Algo-Bio® administration was carried out over a period of 5 days (D0-D4) at a dose of 1...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.01.008

    authors: Kouadio IK,Guessennd N,Dadié A,Koffi E,Dosso M

    更新日期:2018-06-01 00:00:00

  • Emergence in Japan of an isolate of Klebsiella pneumoniae co-harbouring blaKPC-2 and rmtB.

    abstract:OBJECTIVES:Here we describe a clinical isolate of Klebsiella pneumoniae ST11 harbouring both blaKPC-2 and rmtB genes in Japan. METHODS:A carbapenem- and aminoglycoside-resistant K. pneumoniae was isolated from an inpatient in Japan. Whole-genome sequencing (WGS) was performed using an Illumina next-generation sequence...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.11.026

    authors: Uchida H,Tada T,Tohya M,Sugahara Y,Kato A,Miyairi I,Kirikae T

    更新日期:2019-06-01 00:00:00

  • Antimicrobial susceptibility and mechanisms of resistance of Greek Clostridium difficile clinical isolates.

    abstract:OBJECTIVES:This study examined the antimicrobial susceptibility and resistance mechanisms of Clostridium difficile recovered in Greek hospitals during 2012-2015. METHODS:C. difficile isolates (n=88) were collected from clinically-confirmed C. difficile infection from symptomatic patients in 10 Greek hospitals. Minimum...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.09.009

    authors: Chatedaki C,Voulgaridi I,Kachrimanidou M,Hrabak J,Papagiannitsis CC,Petinaki E

    更新日期:2019-03-01 00:00:00