Abstract：
OBJECTIVES:With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance of the carbapenem inactivation method (CIM) and the Carba NP test in the detection of carbapenemase-producers. METHODS:A panel of 203 Gram-negative bacterial isolates screened for carbapenem resistance were subjected to the CIM and Carba NP test. The results were compared with multiplex PCR targeting various carbapenemase genes. RESULTS:According to multiplex PCR, 92 (45.3%) of 203 isolates were found to harbour one or more carbapenemase genes, with blaNDM and blaKPC being the most commonly encountered. The sensitivity and specificity of the CIM were 95.7% and 95.5% respectively, whilst those of the Carba NP test were 75.0% and 99.1%, respectively. Both methods were found to be rapid and reliable in the detection of carbapenemases and showed a high agreement with multiplex PCR. CONCLUSIONS:As the list of carbapenemase genes continues to expand, the reliability of PCR has become doubtful; hence, the CIM and Carba NP test could offer promising alternatives, with the CIM being of a lower cost and less labour intensive.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Madkour LA,Soliman MS,Hassan DM,Soliman NS,ElMahdy YAdoi
10.1016/j.jgar.2016.12.015subject
Has Abstractpub_date
2017-06-01 00:00:00pages
10-14eissn
2213-7165issn
2213-7173pii
S2213-7165(17)30030-9journal_volume
9pub_type
杂志文章abstract：:Various molecular methods have been developed to rapidly detect clarithromycin (CLR) resistance in Helicobacter pylori isolates in clinical specimens. All of these assays for detecting CLR resistance in H. pylori are based on detection of mutations in the 23S rRNA gene. In this article, we summarise current knowledge ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2016.01.002
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abstract：:The two closely related mycobacteria, Mycobacterium intracellulare and Mycobacterium chimaera, exhibit a more than two-fold difference in their in vitro susceptibility to sulfonamides. Sulfonamides are antibiotics targeting the 6-hydroxymethyl-7,8-dihydropteroate synthase (DHPS) enzyme involved in the folate synthesis...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2013.05.003
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) compared with non-HCWs at Duhok city, Kurdistan Region, northern Iraq. METHODS:A total of 182 HCWs with different occupations and working in different...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.01.010
更新日期：2017-06-01 00:00:00
abstract：:The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sens...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.05.007
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:The aim of study was to evaluate by comparative study the level of selection of antibiotic-resistant Enterobacteriaceae in the digestive microbiota of piglets when using amoxicillin and Algo-Bio®. METHODS:Amoxicillin and Algo-Bio® administration was carried out over a period of 5 days (D0-D4) at a dose of 1...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.01.008
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abstract：:Klebsiella pneumoniae carrying blaCTX-M-15 have been widely disseminated in hospital settings. In this regard, most clinically important strains belong to clonal complex 28 (CC258), which includes sequence type 340 (ST340). In this study, we present the draft genome sequence of a CTX-M-15-producing ST340 K. pneumoniae...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.07.012
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVES:This study investigated trends and factors associated with antimicrobial resistance (AMR) in Acinetobacter spp. in Europe. METHODS:Using data from EARS-Net, population-weighted multilevel logistic regression models with random intercepts for each participating country were performed to assess trends in Acin...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.05.024
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVES:Carbapenem-resistant Acinetobacter baumannii (CRAB) have emerged as a serious threat to public-health worldwide. This study aimed to determine the antimicrobial susceptibility of A. baumannii isolates in Iran and to investigate oxacillinase-encoding determinants and their association with insertion sequence ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.12.011
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abstract：:Acinetobacter baumannii coccobacilli are dangerous to patients in intensive care units because of their multidrug resistance to antibiotics, developed mainly in the past decade. This study aimed to examine whether there is a significant correlation between the number of Pro-Ala repeats in the CAP01997 protein, the Emr...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.09.004
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVES:Staphylococcus cohnii has become increasingly recognized as a potential pathogen of clinically significant nosocomial and farm animal infections. This study was designed to determine the genome of a multidrug-resistant S. cohnii subsp. urealyticus strain SNUDS-2 isolated from a farmed duck in Korea. METHODS...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.05.008
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities. The aim of this study was to analyse the resistance, virulence, and epidemiological and genetic relationships of S. aureus from bloodstream infections (BSIs) and pneumonia from patients in Souther...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.07.009
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.03.002
更新日期：2019-12-01 00:00:00
abstract：OBJECTIVES:Multidrug-resistant (MDR) enterococci are found extensively in food samples. This study characterized the phenotypic virulence factors and the ability of horizontal gene transfer of a streptomycin resistance gene among enterococci isolated from fermented pork. METHODS:Thirty-six MDR enterococci were subject...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.04.016
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:Thousands of cases of multidrug-resistant tuberculosis (TB) have been observed in the Philippines, but studies on the Mycobacterium tuberculosis (MTB) genotypes that underlie the observed drug resistance profiles are lacking. This study aimed to analyse the whole genomes of clinical MTB isolates representing...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.08.009
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVES:Recently, the virulence factor sasX was described on the mobile genetic element φSPβ prophage in a ST239-SCCmecIII methicillin-resistant Staphylococcus aureus (MRSA) clone. The aim of this study was to identify sesI, an sasX homologue, in an MRSA ST239 strain. METHODS:MRSA strain VB1490 was isolated from a ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.02.006
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVES:Excessive use of antimicrobial agents for acute upper respiratory infections (URIs) is one of the most common problems in clinical practice. Interventions are required to reduce antimicrobial overuse in these common infections. METHODS:During a 9-month study period (June 2014-February 2015), a quasi-experim...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.08.015
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVES:Multidrug-resistant (MDR) bacteria are a major public-health concern. Bacteriophage endolysins (lysins) can be used as novel antimicrobial agents against bacterial infections. In this study, a novel endolysin (LysSS) containing a lysozyme-like domain was evaluated for its antibacterial activity against vario...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.01.005
更新日期：2020-09-01 00:00:00
abstract：:Mycobacterium tuberculosis is naturally resistant to clarithromycin (CLR). The genes Rv3197A (whiB7) and Rv1988 (ermMT) have been shown to be involved in the resistant phenotype. In this study, a CLR-susceptible M. tuberculosis clinical strain was identified, designated as DS3214, and the nucleotide sequences and expr...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2015.07.004
更新日期：2015-12-01 00:00:00
abstract：OBJECTIVE:Little is known about the treatment outcomes of HIV-negative drug-resistant tuberculosis (TB) patients in Malaysia. With respect to this issue, this study aimed to determine factors associated with unsuccessful treatment outcomes among drug-resistant TB patients at the Institute of Respiratory Medicine, Kuala...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2019.05.009
更新日期：2019-12-01 00:00:00
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journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.12.007
更新日期：2018-09-01 00:00:00
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journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2020.11.003
更新日期：2020-11-24 00:00:00
abstract：:Acinetobacter calcoaceticus-baumannii complex is a common cause of hospital-acquired infections (HAIs) globally, remarkable for its high rate of antibiotic resistance, including to carbapenems. There are few data on the resistance of A. baumannii in Vietnam, which are essential for developing evidence-based treatment ...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2014.05.003
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVES:Scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. The present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important Saudi scorpions (Androcton...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2017.03.008
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVES:The widespread dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae has become a major issue in veterinary medicine. However, until now, there has been no report of bacteria with such a phenotype in infected snakes. The aim of this study was to report the first draft genome sequence of...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.11.009
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVES:Multidrug-resistant Mycobacterium tuberculosis poses a global threat, particularly in developing countries such as Pakistan. Genome sequencing, comparative genomic analysis and drug resistance gene analysis could be beneficial for understanding and monitoring M. tuberculosis disease severity in Pakistan by e...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2018.04.014
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to validate the use of LML antimicrobial gradient strips for quantitative determination of carbapenem susceptibility in Enterobacteriaceae. METHODS:A total of 95 non-redundant Enterobacteriaceae strains isolated during 2012-2014 were used for this validation study. Initially, LML a...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.10.010
更新日期：2017-03-01 00:00:00
abstract：:Acquisition of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) strains poses a major threat to critically ill patients. The objectives of this study were to describe the epidemiology of CP-Kp isolates as well as the clinical outcome associated with the corresponding infections and to identify risk factors for mo...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2015.01.006
更新日期：2015-06-01 00:00:00
abstract：:The objective of this review was to describe the 'state of the art' of Plasmodium falciparum resistance to the main antimalarial drugs. A brief note on Plasmodium vivax is also included. Resistance of P. falciparum to the various antimalarials has a long history of hits and misses. During the last 60 years, the pace a...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2015.02.002
更新日期：2015-06-01 00:00:00
abstract：:The emergence of drug-resistant tuberculosis (TB) is a major public health threat. However, little is known about the predictors of death in drug-resistant TB in Malaysia. This study aimed to determine the predictors of death in drug-resistant TB patients, including multidrug-resistant TB (MDR-TB), in Kuala Lumpur, Ma...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章
doi：10.1016/j.jgar.2016.04.005
更新日期：2016-09-01 00:00:00
abstract：:Environmental fungicides are used in agriculture to reduce fungal spoilage of crops to a minimum, and the polyene macrolide natamycin is used as a food preservative. The use of natamycin in yoghurt has recently been authorised in the USA and some other countries. However, resistance development is a serious risk assoc...
journal_title：Journal of global antimicrobial resistance
pub_type： 杂志文章,评审
doi：10.1016/j.jgar.2017.10.024
更新日期：2018-06-01 00:00:00
