Abstract：
:The suprachiasmatic nucleus houses the central circadian clock and is characterized by the timely regulated expression of clock genes. However, neurons of the cerebellar cortex also contain a circadian oscillator with circadian expression of clock genes being controlled by the suprachiasmatic nucleus. It has been suggested that the cerebellar circadian oscillator is involved in food anticipation, but direct molecular evidence of the role of the circadian oscillator of the cerebellar cortex is currently unavailable. To investigate the hypothesis that the circadian oscillator of the cerebellum is involved in circadian physiology and food anticipation, we therefore by use of Cre-LoxP technology generated a conditional knockout mouse with the core clock gene Arntl deleted specifically in granule cells of the cerebellum, since expression of clock genes in the cerebellar cortex is mainly located in this cell type. We here report that deletion of Arntl heavily influences the molecular clock of the cerebellar cortex with significantly altered and arrhythmic expression of other central clock and clock-controlled genes. On the other hand, daily expression of clock genes in the suprachiasmatic nucleus was unaffected. Telemetric registrations in different light regimes did not detect significant differences in circadian rhythms of running activity and body temperature between Arntl conditional knockout mice and controls. Furthermore, food anticipatory behavior did not differ between genotypes. These data suggest that Arntl is an essential part of the cerebellar oscillator; however, the oscillator of the granular layer of the cerebellar cortex does not control traditional circadian parameters or food anticipation.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Bering T,Carstensen MB,Rath MFdoi
10.1111/jnc.14128subject
Has Abstractpub_date
2017-09-01 00:00:00pages
841-856issue
6eissn
0022-3042issn
1471-4159journal_volume
142pub_type
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1046/j.1471-4159.1995.64052332.x
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2018-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1995.65062716.x
更新日期：1995-12-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1046/j.1471-4159.2002.00802.x
更新日期：2002-05-01 00:00:00
