Abstract:
:Abstract Dorfin is a RING-finger type ubiquitin ligase for mutant superoxide dismutase 1 (SOD1) that enhances its degradation. Mutant SOD1s cause familial amyotrophic lateral sclerosis (FALS) through the gain of unelucidated toxic properties. We previously showed that the accumulation of mutant SOD1 in the mitochondria triggered the release of cytochrome c, followed by the activation of the caspase cascade and induction of neuronal cell death. In the present study, therefore, we investigated whether Dorfin can modulate the level of mutant SOD1 in the mitochondria and subsequent caspase activation. We showed that Dorfin significantly reduced the amount of mutant SOD1 in the mitochondria, the release of cytochrome c and the activation of the following caspase cascade, thereby preventing eventual neuronal cell death in a neuronal cell model of FALS. These results suggest that reducing the accumulation of mutant SOD1 in the mitochondria may be a new therapeutic strategy for mutant SOD1-associated FALS, and that Dorfin may play a significant role in this.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Takeuchi H,Niwa J,Hishikawa N,Ishigaki S,Tanaka F,Doyu M,Sobue Gdoi
10.1046/j.1471-4159.2003.02289.xkeywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
64-72issue
1eissn
0022-3042issn
1471-4159pii
JNC2289journal_volume
89pub_type
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