A simple radioenzymatic method to measure picogram amounts of DOPA in brain and biological fluids.

abstract：:Mechanical strain in neural tissues can lead to the up-regulation and release of multiple cytokines including interleukin 6 (IL-6). In the retina, the mechanosensitive release of ATP can autostimulate P2X7 receptors on both retinal ganglion cell neurons and optic nerve head astrocytes. Here, we asked whether the purin...

journal_title：Journal of neurochemistry

authors： Lu W,Albalawi F,Beckel JM,Lim JC,Laties AM,Mitchell CH

更新日期：2017-05-01 00:00:00

abstract：:Tau is a microtubule-associated protein whose transcript undergoes complex regulated splicing in the mammalian nervous system. Exon 6 of the gene is an alternatively spliced cassette whose expression pattern and splicing regulation had not been previously analyzed in the human. The expression profile of exon 6 is comp...

journal_title：Journal of neurochemistry

authors： Wei ML,Andreadis A

更新日期：1998-04-01 00:00:00

abstract：:Prostaglandin E2 (PGE2 ) plays crucial roles in managing microglial activation through the prostanoid EP2 receptor, a PGE2 receptor subtype. In this study, we report that PGE2 enhances interferon-γ (IFN-γ)-induced nitric oxide production in microglia. IFN-γ increased the release of nitrite, a metabolite of nitric oxid...

journal_title：Journal of neurochemistry

authors： Nagano T,Nishiyama R,Sanada A,Mutaguchi Y,Ioku A,Umeki H,Kishimoto S,Yamanaka D,Kimura SH,Takemura M

更新日期：2017-02-01 00:00:00

abstract：:EAAC1/EAAT3 is a transporter of glutamate (Glu) present at the post-synaptic neuronal element, in opposition to the two other main transporters, GLAST/EAAT1 and GLT1/EAAT2, expressed at the excitatory amino acid (EAA) synapse by surrounding astrocytes. Although, in the adult, EAAC1/EAAT3 exhibits a rather low expressi...

journal_title：Journal of neurochemistry

authors： Nieoullon A,Canolle B,Masmejean F,Guillet B,Pisano P,Lortet S

更新日期：2006-08-01 00:00:00

abstract：:Previous studies point to quaternary assembly of dopamine transporters (DATs) in oligomers. However, it is not clear whether the protomers function independently in the oligomer. Is each protomer an entirely separate unit that takes up dopamine and is inhibited by drugs known to block DAT function? In this work, human...

journal_title：Journal of neurochemistry

authors： Zhen J,Antonio T,Cheng SY,Ali S,Jones KT,Reith ME

更新日期：2015-04-01 00:00:00

abstract：:Cyclooxygenases (COX)-1 and -2 are key enzymes required for the conversion of arachidonic acid to eicosanoids, potent mediators of inflammation. In patients with multiple sclerosis, COX-2 derived prostaglandins (PGs) are elevated in the CSF and COX-2 is up-regulated in demyelinating plaques. However, it is not known w...

journal_title：Journal of neurochemistry

authors： Palumbo S,Toscano CD,Parente L,Weigert R,Bosetti F

更新日期：2012-05-01 00:00:00

abstract：:The proteasome is a multiprotein complex that is involved in the intracellular protein degradation in eukaryotes. Here, we show that human malignant glioma cells are susceptible to apoptotic cell death induced by the proteasome inhibitors, MG132 and lactacystin. The execution of the apoptotic death program involves th...

journal_title：Journal of neurochemistry

authors： Wagenknecht B,Hermisson M,Groscurth P,Liston P,Krammer PH,Weller M

更新日期：2000-12-01 00:00:00

abstract：:Ca2+ uptake into the endoplasmic reticulum (ER) is mediated by Ca2+ ATPase isoforms, which are all selectively inhibited by nanomolar concentrations of thapsigargin. Using ATP/Mg2+-dependent 45Ca2+ transport in rat brain microsomes, tissue sections, and permeabilized cells, as well as Ca2+ imaging in living cells we d...

journal_title：Journal of neurochemistry

authors： Watson WD,Facchina SL,Grimaldi M,Verma A

更新日期：2003-10-01 00:00:00

abstract：:beta-Arrestin 1-GFP or beta-arrestin 2-GFP were coexpressed transiently with G protein-coupled receptor kinase 2 within cells stably expressing the orexin-1, apelin or melanin-concentrating hormone (MCH), receptors. In response to agonist ligands both the orexin-1 and apelin receptors were able to rapidly translocate ...

journal_title：Journal of neurochemistry

authors： Evans NA,Groarke DA,Warrack J,Greenwood CJ,Dodgson K,Milligan G,Wilson S

更新日期：2001-04-01 00:00:00

abstract：:Stressful treatments and immune challenges have been shown previously to elevate brain concentrations of tryptophan. The role of the autonomic nervous system in this neurochemical change was investigated using pharmacological treatments that inhibit autonomic effects. Pretreatment with the ganglionic blocker chlorison...

journal_title：Journal of neurochemistry

authors： Dunn AJ,Welch J

更新日期：1991-11-01 00:00:00

abstract：:The aim of this study was to investigate the effect of long-term treatment with interferon (IFN)-alpha on the noradrenaline transporter of bovine adrenal medullary cells. Treatment of cultured adrenal medullary cells with IFN-alpha caused a decrease in uptake of [3H]noradrenaline by the cells in time (4-48 h)- and con...

journal_title：Journal of neurochemistry

authors： Toyohira Y,Yanagihara N,Minami K,Ueno S,Uezono Y,Tachikawa E,Kondo Y,Kashimoto T,Izumi F

更新日期：1998-04-01 00:00:00

abstract：:We investigated [(18)F]fluoroethyl-harmol ([(18)F]FEH) as a reversible and selective ligand for positron emission tomography (PET) studies of monoamine oxidase A (MAO-A). Binding of [(18)F]FEH in rat brain cryostat sections indicated high affinity (KD = 3 nM), and density (Bmax; 600 pmol/g). The plasma free fraction w...

journal_title：Journal of neurochemistry

authors： Maschauer S,Haller A,Riss PJ,Kuwert T,Prante O,Cumming P

更新日期：2015-12-01 00:00:00

abstract：:Tumor necrosis factor-alpha is a pluripotent cytokine that is reportedly mitogenic to astrocytes. We examined expression of the astrocyte intermediate filament component glial fibrillary acidic protein in astrocyte cultures and the U373 glioblastoma cell line after treatment with tumor necrosis factor-alpha. Treatment...

journal_title：Journal of neurochemistry

authors： Murphy GM Jr,Lee YL,Jia XC,Yu AC,Majewska A,Song Y,Schmidt K,Eng LF

更新日期：1995-12-01 00:00:00

abstract：:Within the cell membrane there exist various microdomains (lipid rafts) in which specific lipids and proteins are assembled and these microdomains are recovered in the detergent-resistant low-density membrane fraction (DRM). Septin is a novel GTP-binding, cytoskeletal protein having various isoforms that assemble into...

journal_title：Journal of neurochemistry

authors： Maimaitiyiming M,Kumanogoh H,Nakamura S,Nagata K,Suzaki T,Maekawa S

更新日期：2008-08-01 00:00:00

abstract：:Lactoferrin (Lf) is an iron-binding protein involved in host defense against infection and severe inflammation, which accumulates in the brain during neurodegenerative disorders. Prior to determining Lf function in pathological brain tissues, we investigated its transport through the blood-brain barrier (BBB) in infla...

journal_title：Journal of neurochemistry

authors： Fillebeen C,Dehouck B,Benaïssa M,Dhennin-Duthille I,Cecchelli R,Pierce A

更新日期：1999-12-01 00:00:00

abstract：:We describe here two types of apoptotic cell death observed in the rat CNS-derived neuroblastoma B50 and B104 cells. One type was induced by dibutyryl cyclic AMP (DBcAMP) after differentiation, and the other was induced by treatment of proliferating cells with cycloheximide. When B50 and B104 cells were treated with 1...

journal_title：Journal of neurochemistry

authors： Honma N,Uchida A,Hirose H,Srsen V,Kishimoto T,Hisanaga S

更新日期：1996-11-01 00:00:00

abstract：:The time course of Ca2+-dependent [3H]acetylcholine [( 3H]ACh) release and inactivation of 45Ca2+ entry were examined in rat brain synaptosomes depolarized by 45 mM [K+]0. Under conditions where the intrasynaptosomal stores of releasable [3H]ACh were neither exhausted nor replenished in the course of stimulation, the ...

journal_title：Journal of neurochemistry

authors： Suszkiw JB,O'Leary ME

更新日期：1983-09-01 00:00:00

abstract：:Several mechanisms are thought to be involved in the progressive decline in neurons of the substantia nigra pars compacta (SNpc) that leads to Parkinson's disease (PD). Neurotoxin 6-hydroxydopamine (6-OHDA), which induces parkinsonian symptoms in experimental animals, is thought to be formed endogenously in patients w...

journal_title：Journal of neurochemistry

authors： Barkats M,Millecamps S,Bilang-Bleuel A,Mallet J

更新日期：2002-07-01 00:00:00

abstract：:The effectiveness of intranasal drug administration to stimulate central neuronal systems is well known from drug addiction and has also been considered as an alternative pharmacokinetic approach to treat brain disorders such as Parkinson's disease. In the present study, the possible neurochemical effects of intranasa...

journal_title：Journal of neurochemistry

authors： De Souza Silva MA,Mattern C,Häcker R,Nogueira PJ,Huston JP,Schwarting RK

更新日期：1997-01-01 00:00:00

abstract：:Tissue plasminogen activator (tPA) is necessary for hippocampal long-term potentiation. Synaptically released zinc also contributes to long-term potentiation, especially in the hippocampal CA3 region. Using cortical cultures, we examined whether zinc increased the concentration and/or activity of tPA. Two hours after ...

journal_title：Journal of neurochemistry

authors： Hwang IY,Sun ES,An JH,Im H,Lee SH,Lee JY,Han PL,Koh JY,Kim YH

更新日期：2011-09-01 00:00:00

abstract：:5-Hydroxytryptamine (5-HT) receptors contain seven putative transmembrane domains and couple via different guanine nucleotide binding proteins to specific effector enzymes. Studies with other receptors identify the second and third intracellular loops or the C-terminus of the receptor as important for selective effect...

journal_title：Journal of neurochemistry

authors： Oksenberg D,Havlik S,Peroutka SJ,Ashkenazi A

更新日期：1995-04-01 00:00:00

abstract：:Excess copper exposure is thought to be linked to the development of Alzheimer's disease (AD) neuropathology. However, the mechanism by which copper affects the CNS remains unclear. To investigate the effect of chronic copper exposure on both beta-amyloid and tau pathologies, we treated young triple transgenic (3xTg-A...

journal_title：Journal of neurochemistry

authors： Kitazawa M,Cheng D,Laferla FM

更新日期：2009-03-01 00:00:00

abstract：:The normal developmental profile of preprotachykinin (PPT) gene expression was determined in rat striatum from embryonic day 20 to adulthood (greater than 45 days of age). At embryonic day 20, the amount of striatal PPT mRNA was approximately 8% of adult levels, and this specific mRNA approached adult levels by postna...

journal_title：Journal of neurochemistry

authors： Haverstick DM,Jeziorski M,Bannon MJ

更新日期：1990-09-01 00:00:00

abstract：:Transcriptional responses to growth factor and G protein-coupled receptors were compared in PC12 cells using retroviral luciferase reporters. In cells stably expressing alpha(1A)-adrenergic receptors, norepinephrine activated all five reporters [AP1 (activator protein-1), SRE (serum response element), CRE (cyclic AMP ...

journal_title：Journal of neurochemistry

authors： Minneman KP,Lee D,Zhong H,Berts A,Abbott KL,Murphy TJ

更新日期：2000-06-01 00:00:00

abstract：:Cultures of bovine adrenomedullary chromaffin cells accumulated 1-[methyl-3H]methyl-4-phenylpyridinium ([3H]MPP+) in a time- and concentration-dependent manner with an apparent Km of 0.7 microM and a Vmax of 3 pmol/min/10(6) cells. The uptake was sodium dependent and sensitive to inhibitors of the cell-surface catecho...

journal_title：Journal of neurochemistry

authors： Reinhard JF Jr,Diliberto EJ Jr,Daniels AJ

更新日期：1989-04-01 00:00:00

abstract：:Microglial activation by blood-borne factors following blood-brain barrier damage may play a significant role in subsequent neuropathogenesis of several neurodegenerative diseases. Exposure of primary cultured rat brain microglia to pure, fatty acid- and lipid-deficient rat serum albumin or fraction V, (fatty acid and...

journal_title：Journal of neurochemistry

authors： Hooper C,Pinteaux-Jones F,Fry VA,Sevastou IG,Baker D,Heales SJ,Pocock JM

更新日期：2009-05-01 00:00:00

abstract：:The activity-dependent fusion, retrieval and recycling of synaptic vesicles is essential for the maintenance of neurotransmission. Until relatively recently it was believed that most mutations in genes that were essential for this process would be incompatible with life, because of this fundamental role. However, an e...

journal_title：Journal of neurochemistry

authors： Bonnycastle K,Davenport EC,Cousin MA

更新日期：2020-05-07 00:00:00

abstract：:Previously used methods of comparing amperometric spike characteristics from two separate groups of cells have entailed pooling all the values for a spike characteristic from each group of cells and then statistically comparing the two samples. Although this approach has indicated that there are significant difference...

journal_title：Journal of neurochemistry

authors： Colliver TL,Hess EJ,Pothos EN,Sulzer D,Ewing AG

更新日期：2000-03-01 00:00:00

abstract：:The regulation of glial and neuronal Na+-dependent glutamate/aspartate transporters is of interest because abnormal glutamate transport may be responsible for certain neurological diseases. Because expression of the Wnt-1 protooncogene results in induction of the glial-type glutamate transporter GLAST in PC12 neuron-l...

journal_title：Journal of neurochemistry

authors： Palos TP,Zheng S,Howard BD

更新日期：1999-09-01 00:00:00

abstract：:The Na(+)-glutamate cotransporters are believed to countertransport OH- and K+. Previous evidence that the velocity of glutamate uptake can exceed the acid extrusion capacity of astrocytes raised the question of whether intracellular pH can become rate limiting for glutamate uptake. Cytoplasmic buffering capacity and ...

journal_title：Journal of neurochemistry

authors： Judd MG,Nagaraja TN,Brookes N

更新日期：1996-01-01 00:00:00