Two possibly distinct prostaglandin E1 receptors in N1E-115 clone: one mediating inositol trisphosphate formation, cyclic GMP formation, and intracellular calcium mobilization and the other mediating cyclic AMP formation.


:Prostaglandin E1 (PGE1)-mediated transmembrane signal control systems were investigated in intact murine neuroblastoma cells (clone N1E-115). PGE1 increased intracellular levels of total inositol phosphates (IP), cyclic GMP, cyclic AMP, and calcium ([Ca2+]i). PGE1 transiently increased inositol 1,4,5-trisphosphate formation, peaking at 20 s. There was more than a 10-fold difference between the ED50 for PGE1 at cyclic AMP formation (70 nM) and its ED50 values at IP accumulation (1 microM), cyclic GMP formation (2 microM), and [Ca2+]i increase (5 microM). PGE1-mediated IP accumulation, cyclic GMP formation, and [Ca2+]i increase depended on both the concentration of PGE1 and extracellular calcium ions. PGE1 had more potent intrinsic activity in cyclic AMP formation, IP accumulation, and cyclic GMP formation than did PGE2, PGF2 alpha, or PGD2. A protein kinase C activator, 4 beta-phorbol 12 beta-myristate 13 alpha-acetate, had opposite effects on PGE1-mediated IP release and cyclic GMP formation (inhibitory) and cyclic AMP formation (stimulatory). These data suggest that there may be subtypes of the PGE1 receptor in this clone: a high-affinity receptor mediating cyclic AMP formation, and a low-affinity receptor mediating IP accumulation, cyclic GMP formation, and intracellular calcium mobilization.


J Neurochem


Kanba S,Sasakawa N,Nakaki T,Kanba KS,Yagi G,Kato R,Richelson E





Has Abstract


1991-12-01 00:00:00












  • Tumor necrosis factor receptor-associated factor 6 participates in early brain injury after subarachnoid hemorrhage in rats through inhibiting autophagy and promoting oxidative stress.

    abstract::Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a member of the TRAF family and an important multifunctional intracellular adaptin of the tumor necrosis factor superfamily and toll/IL-1 receptor (TIR) superfamily. TRAF6 has been studied in several central nervous system diseases, including ischemic strok...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Dou Y,Shen H,Feng D,Li H,Tian X,Zhang J,Wang Z,Chen G

    更新日期:2017-08-01 00:00:00

  • Biogenesis of presynaptic terminal proteins.

    abstract::The delivery of proteins to the presynaptic terminals of guinea pig retinal ganglion cells by two of the major components of axonal transport, and the subsequent persistence and turnover of those proteins were examined in this study. Ganglion cell proteins were radiolabeled by intravitreal injection of radiolabeled am...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Garner JA,Mahler HR

    更新日期:1987-09-01 00:00:00

  • Calcium/calmodulin-dependent serine protein kinase (CASK) is a new intracellular modulator of P2X3 receptors.

    abstract::ATP-gated P2X3 receptors of sensory ganglion neurons are important transducers of painful stimuli and are modulated by extracellular algogenic substances, via changes in the receptor phosphorylation state. The present study investigated the role of calcium/calmodulin-dependent serine protein kinase (CASK) in interacti...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gnanasekaran A,Sundukova M,Hullugundi S,Birsa N,Bianchini G,Hsueh YP,Nistri A,Fabbretti E

    更新日期:2013-07-01 00:00:00

  • Impaired proteasome activity and accumulation of ubiquitinated substrates in a hereditary neuropathy model.

    abstract::Accumulation of misfolded proteins and alterations in the ubiquitin-proteasome pathway are associated with various neurodegenerative conditions of the CNS and PNS. Aggregates containing ubiquitin and peripheral myelin protein 22 (PMP22) have been observed in the Trembler J mouse model of Charcot-Marie-Tooth disease ty...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fortun J,Li J,Go J,Fenstermaker A,Fletcher BS,Notterpek L

    更新日期:2005-03-01 00:00:00

  • Exosomes in Parkinson disease.

    abstract::Parkinson disease (PD) is a prevalent neurodegenerative disease, in which the formation of misfolded and aggregated α-synuclein is a key neuropathological hallmark. Recent studies reveal that extracellular vesicles such as exosomes present a potential mechanism for propagation of pathological α-synuclein throughout th...

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    pub_type: 杂志文章,评审


    authors: Pinnell JR,Cui M,Tieu K

    更新日期:2020-12-29 00:00:00

  • Inhibition of glucosylceramide synthase stimulates autophagy flux in neurons.

    abstract::Aggregate-prone mutant proteins, such as α-synuclein and huntingtin, play a prominent role in the pathogenesis of various neurodegenerative disorders; thus, it has been hypothesized that reducing the aggregate-prone proteins may be a beneficial therapeutic strategy for these neurodegenerative disorders. Here, we ident...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shen W,Henry AG,Paumier KL,Li L,Mou K,Dunlop J,Berger Z,Hirst WD

    更新日期:2014-06-01 00:00:00

  • A G-rich element forms a G-quadruplex and regulates BACE1 mRNA alternative splicing.

    abstract::β-Site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) is the transmembrane aspartyl protease that catalyzes the first cleavage step in the proteolysis of the APP to the amyloid β-protein (Aβ), a process involved in the pathogenesis of Alzheimer disease. BACE1 pre-mRNA undergoes complex alternative splicing,...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fisette JF,Montagna DR,Mihailescu MR,Wolfe MS

    更新日期:2012-06-01 00:00:00

  • Characterization of bradykinin-induced phosphoinositide turnover in neurohybrid NCB-20 cells.

    abstract::Phosphoinositide hydrolysis was studied in neurohybrid NCB-20 cells prelabeled with myo-[3H]inositol. Among nearly 20 neurotransmitters and neuromodulators examined, only bradykinin, carbachol, and histamine significantly increased the accumulation of [3H]inositol monophosphate (IP1) in the presence of lithium. The EC...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chuang DM,Dillon-Carter O

    更新日期:1988-08-01 00:00:00

  • Functional mapping of dorsal and median raphe 5-hydroxytryptamine pathways in forebrain of the rat using microdialysis.

    abstract::Recent neurochemical studies of the properties of 5-hydroxytryptamine (5-HT) pathways arising from the dorsal raphe nucleus (DRN) and median raphe nucleus (MRN) have measured extracellular 5-HT in brain regions with reported preferential DRN or MRN 5-HT inputs. Here, we have tested whether electrical stimulation of th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: McQuade R,Sharp T

    更新日期:1997-08-01 00:00:00

  • Differential inactivation of postsynaptic density-associated and soluble Ca2+/calmodulin-dependent protein kinase II by protein phosphatases 1 and 2A.

    abstract::Autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) at Thr286 generates Ca2+-independent activity. As an initial step toward understanding CaMKII inactivation, protein phosphatase classes (PP1, PP2A, PP2B, or PP2C) responsible for dephosphorylation of Thr286 in rat forebrain subcellular fractio...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Strack S,Barban MA,Wadzinski BE,Colbran RJ

    更新日期:1997-05-01 00:00:00

  • Peptidase regulation of gonadotropin-releasing hormone levels during in vitro incubations of the rat hypothalamus.

    abstract::The fate of gonadotropin-releasing hormone (GnRH) was examined by a GnRH radioimmunoassay during in vitro incubations of the rat medial basal hypothalamus (MBH). There was a progressive disappearance of exogenous GnRH during MBH incubations. The GnRH degradation could be explained by the release of peptidases from the...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Powers CA,Johnson DC

    更新日期:1981-02-01 00:00:00

  • Regulation of actin and its mRNA by thyroid hormones in cultures of fetal human brain during second trimester of gestation.

    abstract::The effect of thyroid hormones (THs) on the expression of actin gene during fetal human brain development and the period of sensitivity to the hormones have been investigated. Developmental profile of actin in the cytoskeletal (CSK) and noncytoskeletal (non-CSK) fractions in the fetal cerebra showed a pronounced rise ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pal U,Biswas SC,Sarkar PK

    更新日期:1997-09-01 00:00:00

  • Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors.

    abstract::beta-Arrestin 1-GFP or beta-arrestin 2-GFP were coexpressed transiently with G protein-coupled receptor kinase 2 within cells stably expressing the orexin-1, apelin or melanin-concentrating hormone (MCH), receptors. In response to agonist ligands both the orexin-1 and apelin receptors were able to rapidly translocate ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Evans NA,Groarke DA,Warrack J,Greenwood CJ,Dodgson K,Milligan G,Wilson S

    更新日期:2001-04-01 00:00:00

  • Functional coupling of the delta-, mu-, and kappa-opioid receptors to mitogen-activated protein kinase and arachidonate release in Chinese hamster ovary cells.

    abstract::To examine whether the mitogen-activated protein kinase (MAPK) cascade and phospholipase A2 (PLA2) are involved in the signal transduction mechanism of the opioid receptor, the delta-, mu-, and kappa-opioid receptors were stably expressed from cDNA in Chinese hamster ovary cells. Activation of the delta-, mu-, and kap...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fukuda K,Kato S,Morikawa H,Shoda T,Mori K

    更新日期:1996-09-01 00:00:00

  • Unbiased proteomic screening identifies a novel role for the E3 ubiquitin ligase Nedd4-2 in translational suppression during ER stress.

    abstract::Endoplasmic reticulum (ER) stress occurs when protein folding or maturation is disrupted. A malfunction in the ER stress response can lead to cell death and has been observed in many neurological diseases. However, how the ER stress response is regulated in neuronal cells remains largely unclear. Here, we studied an E...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Eagleman DE,Zhu J,Liu DC,Seimetz J,Kalsotra A,Tsai NP

    更新日期:2020-10-16 00:00:00

  • In vitro release of endogenous dopamine from the striatum of the weaver mutant mouse.

    abstract::The weaver mutant mouse has a genetically determined defect in the nigrostriatal dopaminergic system. The present study was undertaken to test the hypothesis that in the weaver mutant mouse, striatal nerve terminals undergo compensatory changes in response to this deficiency. To test this hypothesis, we studied the ba...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Simon JR,Yu H,Richter JA,Vasko MR,Ghetti B

    更新日期:1991-11-01 00:00:00

  • [3H]Imipramine is accumulated but not released from slices of the rabbit caudate and hypothalamus.

    abstract::Slices of rabbit caudate and hypothalamus take up and accumulate [3H]imipramine. In superfused slices of both structures electrical stimulation or exposure to tyramine failed to release recently taken up [3H]imipramine. Depolarization by exposure to 30--60 mM-potassium caused only a small release of [3H]imipramine tha...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Langer SZ,Galzin AM,Kamal LA

    更新日期:1982-02-01 00:00:00

  • Plasma desmoplakin I biomarker of vascular recurrence after ischemic stroke.

    abstract::Stroke patients have a high risk of vascular recurrence. Biomarkers related to vascular recurrence, however, remain to be identified. The aim of the study was to identify, through proteomic analysis, plasma biomarkers associated with vascular recurrence within one year after the first ischemic stroke. This is a substu...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,多中心研究


    authors: López-Farré AJ,Zamorano-León JJ,Segura A,Mateos-Cáceres PJ,Modrego J,Rodríguez-Sierra P,Calatrava L,Tamargo J,Macaya C

    更新日期:2012-04-01 00:00:00

  • Expression of multiple AQP4 pools in the plasma membrane and their association with the dystrophin complex.

    abstract::Altered aquaporin-4 (AQP4) expression has been reported in brain edema, tumors, muscular dystrophy, and neuromyelitis optica. However, the plasma membrane organization of AQP4 and its interaction with proteins such as the dystrophin-associated protein complex are not well understood. In this study, we used sucrose den...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Nicchia GP,Cogotzi L,Rossi A,Basco D,Brancaccio A,Svelto M,Frigeri A

    更新日期:2008-06-01 00:00:00

  • Pre-synaptic dopamine D(3) receptor mediates cocaine-induced structural plasticity in mesencephalic dopaminergic neurons via ERK and Akt pathways.

    abstract::Exposure to psychostimulants results in neuroadaptive changes of the mesencephalic dopaminergic system including morphological reorganization of dopaminergic neurons. Increased dendrite arborization and soma area were previously observed in primary cultures of mesencephalic dopaminergic neurons after 3-day exposure to...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Collo G,Bono F,Cavalleri L,Plebani L,Merlo Pich E,Millan MJ,Spano PF,Missale C

    更新日期:2012-03-01 00:00:00

  • Diagnostic and therapeutic potential of exosomes in Alzheimer's disease.

    abstract::Exosomes are small extracellular vesicles released by almost all cell types in physiological and pathological conditions. The exosomal potential to unravel disease mechanisms, or to be used as a source of biomarkers, is being explored, in particularly in the field of neurodegenerative diseases. Alzheimer's disease (AD...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Soares Martins T,Trindade D,Vaz M,Campelo I,Almeida M,Trigo G,da Cruz E Silva OAB,Henriques AG

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  • Reduced glycine stimulation of [3H]MK-801 binding in Alzheimer's disease.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Procter AW,Wong EH,Stratmann GC,Lowe SL,Bowen DM

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  • A negative regulatory element in the rat dopamine beta-hydroxylase gene contributes to the cell type specificity of expression.

    abstract::Dopamine beta-hydroxylase (DBH) catalyzes the synthesis of the neurohormone norepinephrine and is expressed only in noradrenergic and adrenergic cells of the nervous and endocrine systems. We have previously described a positive-acting genetic regulatory element of the DBH that contributes to the restricted expression...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shaskus J,Zellmer E,Lewis EJ

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  • Drug testing complementary metal-oxide-semiconductor chip reveals drug modulation of transmitter release for potential therapeutic applications.

    abstract::Neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease, and Huntington's disease, are considered incurable and significantly reduce the quality of life of the patients. A variety of drugs that modulate neurotransmitter levels have been used for the treatment of the neurodegenerative diseases but ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Huang M,Rathore SS,Lindau M

    更新日期:2019-10-01 00:00:00

  • 1-Piperideine as an in vivo precursor of the gamma-aminobutyric acid homologue 5-aminopentanoic acid.

    abstract::Intraperitoneal injection of the cyclic imine 1-piperideine in mice resulted in measurable quantities of 5-aminopentanoic acid in brain. 5-Aminopentanoic acid is a methylene homologue of gamma-aminobutyric acid (GABA) that is a weak GABA agonist. 5-Aminopentanoic acid formed in the periphery was ruled out as the sourc...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Callery PS,Geelhaar LA

    更新日期:1985-09-01 00:00:00

  • Characterization of a cloned locust tyramine receptor cDNA by functional expression in permanently transformed Drosophila S2 cells.

    abstract::The cDNA for Tyr-Loc, a G protein-coupled receptor that clearly shows homology to a number of mammalian and fruit fly receptors for biogenic amines, was cloned from the nervous system of Locusta migratoria. Functional expression of the cloned cDNA was obtained in cultured insect cells, i.e., in Spodoptera SF9 cells us...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Vanden Broeck J,Vulsteke V,Huybrechts R,De Loof A

    更新日期:1995-06-01 00:00:00

  • Further studies on the nature of postsynaptic dopamine uptake and metabolism in rat striatum: sodium dependency and investigation of a possible role for carrier-mediated uptake into serotonin neurons.

    abstract::The nature of postsynaptic sites involved in the uptake and metabolism of striatal 3,4-dihydroxyphenylethylamine (dopamine, DA) was investigated. The accumulation of [3H]DA (10(-7) M) into slices of rat striatum was found to be greatly dependent (greater than 99%) on the presence of sodium ion in the incubation medium...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schoepp DD,Azzaro AJ

    更新日期:1985-06-01 00:00:00

  • Protein kinase inhibitor H-7 differentially affects early and delayed nerve growth factor responses in PC12 cells.

    abstract::The effects of the protein kinase inhibitor H-7 on early and delayed responses to nerve growth factor (NGF) were investigated in PC12 cells. H-7 reduced the NGF-induced expression of c-Fos in a dose-dependent manner without affecting the time course of c-Fos appearance. Conversely, H-7 potentiated delayed NGF effects,...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kahle P,Mangold M,Kuwahara T,Schubenel R,Hertel C

    更新日期:1994-02-01 00:00:00

  • Inactive monomeric acetylcholinesterase in the low-salt-soluble extract of the electric organ from Torpedo marmorata.

    abstract::Proteolytic fragmentation of [3H]diisopropylfluorophosphate-labelled catalytic subunits of different molecular forms of acetylcholinesterase demonstrates that all forms extracted from the electric organ from Torpedo marmorata are true acetylcholinesterases. This is supported by immunochemical results showing that the ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Stieger S,Brodbeck U,Witzemann V

    更新日期:1987-08-01 00:00:00

  • Differential capacities of the RGS1, RGS16 and RGS-GAIP regulators of G protein signaling to enhance alpha2A-adrenoreceptor agonist-stimulated GTPase activity of G(o1)alpha.

    abstract::Recombinant RGS1, RGS16 and RGS-GAIP, but not RGS2, were able to substantially further stimulate the maximal GTPase activity of G(o1)alpha promoted by agonists at the alpha2A-adrenoreceptor in a concentration-dependent manner. Kinetic analysis of the regulation of an alpha2A-adrenoreceptor-G(o1)alpha fusion protein by...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hoffmann M,Ward RJ,Cavalli A,Carr IC,Milligan G

    更新日期:2001-08-01 00:00:00