Abstract:
:Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lázaro-Díez M,Chapartegui-González I,Redondo-Salvo S,Leigh C,Merino D,Segundo DS,Fernández A,Navas J,Icardo JM,Acosta F,Ocampo-Sosa A,Martínez-Martínez L,Ramos-Vivas Jdoi
10.1038/s41598-017-04870-8subject
Has Abstractpub_date
2017-07-04 00:00:00pages
4571issue
1issn
2045-2322pii
10.1038/s41598-017-04870-8journal_volume
7pub_type
杂志文章abstract::In this study, we measured the human epidermal growth factor receptor 2 (HER2) copy number in both tissue and plasma samples of gastric cancer patients by using droplet digital polymerase chain reaction (ddPCR) method. Eighty gastric cancer patients were enrolled and both formalin-fixed and paraffin-embedded tissue an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60897-4
更新日期:2020-03-06 00:00:00
abstract::In recent years, there has been an impressively fast technological progress in the development of highly efficient lead halide perovskite solar cells. However, the stability of perovskite films and respective solar cells is still an open point of concern and calls for advanced characterization methods. In this work, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35973
更新日期:2016-10-27 00:00:00
abstract::Gadoxetic acid (Gd-EOB-DTPA) is a paramagnetic MRI contrast agent with raising popularity and has been used for evaluation of imaging-based liver function in recent years. In order to verify whether liver function as determined by real-time breath analysis using the intravenous administration of 13C-methacetin can be ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27401-5
更新日期:2018-06-13 00:00:00
abstract::Microphysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion. Here, we report an approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-syste...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22749-0
更新日期:2018-03-14 00:00:00
abstract::Several epidemiological studies have found a positive association between diabetic retinopathy (DR) and subclinical hypothyroidism (SCH), but the findings are varied or even contradictory. In the work, we performed a meta-analysis to ascertain the relationship between DR and SCH. We searched relevant studies on the re...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/srep12212
更新日期:2015-07-20 00:00:00
abstract::The histological spectrum of nonalcoholic fatty liver diseases (NAFLD) ranges from hepatic steatosis to steatohepatitis and fibrosis. Berberine (BBR) is known for its therapeutic effect on obesity, hyperglycaemia and dyslipidaemia; however, its effect on NAFLD has yet to be thoroughly explored. Db/db mice and methioni...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20848
更新日期:2016-02-09 00:00:00
abstract::Conifer wood is an exceptionally stiff and strong material when its cellulose microfibrils are well aligned. However, it is not well understood how the polymer components cellulose, hemicelluloses and lignin co-operate to resist tensile stress in wood. From X-ray scattering, neutron scattering and spectroscopic data, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79676-2
更新日期:2021-01-11 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is a progressive form of non-alcoholic fatty liver disease (NAFLD) that may lead to liver cirrhosis or hepatocellular carcinoma. Here, we examined the diagnostic utility of tri-antennary tri-sialylated mono-fucosylated glycan of alpha-1 antitrypsin (AAT-A3F), a non-invasive glycobi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56947-1
更新日期:2020-01-15 00:00:00
abstract::Strenuous exercise increases circulating cell free DNA (cfDNA) and stimulates blood phagocytes to generate reactive oxygen species (ROS) which may induce DNA strand breaks. We tested whether: (A) elevated cfDNA in response to three repeated bouts of exhaustive exercise has decreased integrity; (B) each bout of exercis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52409-w
更新日期:2019-11-04 00:00:00
abstract::Vitiligo is an acquired depigmentation disorder largely caused by defective melanocyte- or autoimmunity-induced melanocyte destruction. The aryl hydrocarbon receptor (AHR) is essential for melanocyte homeostasis and immune process, and abnormal AHR was observed in vitiligo. We previously identified the T allele of AHR...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13542
更新日期:2015-09-15 00:00:00
abstract::Cardiac tissue engineering using biomaterials with or without combination of stem cell therapy offers a new option for repairing infarcted heart. However, the bioactivity of biomaterials remains to be optimized because currently available biomaterials do not mimic the biochemical components as well as the structural p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39988
更新日期:2017-01-04 00:00:00
abstract::Pulse generation often requires a stabilized cavity and its corresponding mode structure for initial phase-locking. Contrastingly, modeless cavity-free random lasers provide new possibilities for high quantum efficiency lasing that could potentially be widely tunable spectrally and temporally. Pulse generation in rand...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18526
更新日期:2015-12-21 00:00:00
abstract::Understanding interactions between cell-penetrating peptides and biomembrane under tension can help improve drug delivery and elucidate mechanisms underlying fundamental cellular events. As far as the effect of membrane tension on translocation, it is generally thought that tension should disorder the membrane structu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12808
更新日期:2015-08-03 00:00:00
abstract::Inflammatory and cardiovascular biomarkers have been associated with obesity, but little is known about how they change upon dietary intervention and concomitant weight loss. Further, protein biomarkers might be useful for predicting weight loss in overweight and obese individuals. We performed secondary analyses in t...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-64636-7
更新日期:2020-05-13 00:00:00
abstract::This study aimed to determine the role of magnetic resonance imaging (MRI) in diagnosing and describing the characteristics of fistula-in-ano, and the agreement between MRI and operative findings. We conducted a retrospective study in 367 patients with fistula-in-ano who were diagnosed and had an operation at the Univ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54441-2
更新日期:2019-11-29 00:00:00
abstract::We recently showed that spinal cord injury (SCI) leads to a decrease in mRNA editing of serotonin receptor 2C (5-HT2CR) contributing to post-SCI spasticity. Here we study post-SCI mRNA editing and global gene expression using massively parallel sequencing. Evidence is presented that the decrease in 5-HT2CR editing is ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12615
更新日期:2015-07-30 00:00:00
abstract::The role of the different lymphocyte populations in liver microenvironment of chronic hepatitis C (CHC) patients is still matter of debate. Since Th17 and Treg have opposite functions, their balance could affect disease progression. The aim was to explore liver microenvironment and its peripheral blood counterpart in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13777-3
更新日期:2017-10-16 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-59303-w
更新日期:2020-02-06 00:00:00
abstract::Fat deposition of beef cattle varies between breeds. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant difference in fat deposition to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05702-5
更新日期:2017-07-13 00:00:00
abstract::Delivering gene constructs into the dorsal root ganglia (DRG) is a powerful but challenging therapeutic strategy for sensory disorders affecting the DRG and their peripheral processes. The current delivery methods of direct intra-DRG injection and intrathecal injection have several disadvantages, including potential i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35612
更新日期:2016-10-17 00:00:00
abstract::Controlling light and heat via metamaterials has presented interesting technological applications using transformation optics (TO) and transformation thermodynamics (TT). However, such devices are commonly mono-physics and mono-purpose, because the used metamaterial is designed to deal with one type of physical mechan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29866-w
更新日期:2018-07-30 00:00:00
abstract::To achieve a better understanding of the "vinegar syndrome" phenomenon, which has caused serious damage to triacetyl cellulose-based motion picture films, the white powder obtained from damaged film surfaces was analysed in this study. The powder was found to be soluble in acetone, diethyl ether, dimethylformamide, an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80498-5
更新日期:2021-01-15 00:00:00
abstract::Every year, billions of wild diurnal songbirds migrate at night. To do so, they shift their daily rhythm from diurnality to nocturnality. In captivity this is observed as a gradual transition of daytime activity developing into nocturnal activity, but how wild birds prepare their daily rhythms for migration remains la...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34207
更新日期:2016-09-26 00:00:00
abstract::The volcanic eruption of Santorini in the Bronze Age left detectable debris across the Mediterranean, serving as an anchor in time for the region, synchronizing chronologies of different sites. However, dating the eruption has been elusive for decades, as radiocarbon indicates a date about a century earlier than archa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79024-4
更新日期:2021-01-12 00:00:00
abstract::The effect of substitution of Nb by Mo in Nb40Ti30Ni30 was investigated with respect to microstructural features and hydrogen dissolution, diffusion and permeation. As-cast Nb40-xMoxTi30Ni30 (x = 0, 5, 10) alloys consist of primary bcc-Nb phase and binary eutectic (bcc-Nb + B2-TiNi). The substitution of Nb by Mo reduc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00335-0
更新日期:2017-03-16 00:00:00
abstract::This paper portrays the dynamics of pine wilt disease. The specific formula for reproduction number is accomplished. Global behavior is completely demonstrated on the basis of the basic reproduction number [Formula: see text]. The disease-free equilibrium is globally asymptotically stable for [Formula: see text] and i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60088-1
更新日期:2020-02-26 00:00:00
abstract::Moonlighting proteins, including metabolic enzymes acting as transcription factors (TF), are present in a variety of organisms but have not been described in higher fungi so far. In a previous genome-wide analysis of the TF repertoire of the plant-symbiotic fungus Tuber melanosporum, we identified various enzymes, inc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25165
更新日期:2016-04-28 00:00:00
abstract::A culture system that can recapitulate myelination in vitro will not only help us better understand the mechanism of myelination and demyelination, but also find out possible therapeutic interventions for treating demyelinating diseases. Here, we introduce a simple and reproducible myelination culture system using mou...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15122
更新日期:2015-10-12 00:00:00
abstract::It is challenging to create artificial catalysts that approach enzymes with regard to catalytic efficiency and selectivity. The enantioselective catalysis ranks the privileged characteristic of enzymatic transformations. Here, we report two pyridine-linked bis(β-cyclodextrin) (bisCD) copper(II) complexes that enantios...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22080
更新日期:2016-02-26 00:00:00
abstract::Pyrazinamide (PZA) is an important first-line drug in all existing and new tuberculosis (TB) treatment regimens. PZA-resistance in M. tuberculosis is increasing, especially among M/XDR cases. Noted issues with PZA Drug Susceptibility Testing (DST) have driven the search for alternative tests. This study provides a com...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03452-y
更新日期:2017-06-19 00:00:00