System-wide organization of actin cytoskeleton determines organelle transport in hypocotyl plant cells.

abstract：:A highly specific radioimmunoassay for one of the putative adrenomedullary [Met]enkephalin precursors, BAM-12P (Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu-OH), has been developed. The BAM-12P antibodies recognize the COOH-terminal fragment of the peptide from Arg7 to Glu12 and do not crossreact with [Met5]- or [L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baird A,Ling N,Böhlen P,Benoit R,Klepper R,Guillemin R

更新日期：1982-03-01 00:00:00

abstract：:One of the advantages of the production of monoclonal antibodies by tissue culture methods is that they can be internally labeled by using appropriate radioactive amino acid in the culture fluid. Thus, radioactive immunological probes of high specific activity can be prepared. Here we report applications of these inte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuello AC,Priestley JV,Milstein C

更新日期：1982-01-01 00:00:00

abstract：:Whether the mate sampling and choice performed by females in nature influences offspring performance is a controversial issue in theory and an open empirical question. Pronghorn (Antilocapra americana) females engage in an obvious and energetically expensive mate sampling process to identify vigorous males. Although i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Byers JA,Waits L

更新日期：2006-10-31 00:00:00

abstract：:Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar KP,Akoulitchev S,Reinberg D

更新日期：1998-08-18 00:00:00

abstract：:A mutation partially suppressing the UV sensitivity caused by recF143 in a uvrA6 background was located at codon 37 of recA where GTG (valine) became ATG (methionine). This mutation, originally named srf-803, was renamed recA803. Little if any suppression of the recF143 defect in UV induction of a lexA regulon promote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Madiraju MV,Templin A,Clark AJ

更新日期：1988-09-01 00:00:00

abstract：:A single mutation altering the transmembrane domain of the receptor-like p185 protein encoded by the rat neu gene converts the normal neu gene into a potent oncogene. The biochemical consequences of this mutation were studied by examining phosphorylation of the normal and transforming p185 molecules in membrane prepar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bargmann CI,Weinberg RA

更新日期：1988-08-01 00:00:00

abstract：:Niemann-Pick C1 (NPC1) and NPC2 proteins are indispensable for the export of LDL-derived cholesterol from late endosomes. Mutations in these proteins result in Niemann-Pick type C disease, a lysosomal storage disease. Despite recent reports of the NPC1 structure depicting its overall architecture, the function of its ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Lu F,Trinh MN,Schmiege P,Seemann J,Wang J,Blobel G

更新日期：2017-08-22 00:00:00

abstract：:To understand the evolution of a Theory of Mind, we need to understand the selective factors that might have jumpstarted its initial evolution. We argue that a subconscious, reflexive appreciation of others' intentions, emotions, and perspectives is at the roots of even the most complex forms of Theory of Mind and tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seyfarth RM,Cheney DL

更新日期：2013-06-18 00:00:00

abstract：:A study of the near-infrared absorption spectra of three oxygen compounds of membrane-bound cytochrome oxidase (ferrocytochrome c:oxygen oxidoreductase; EC 1.9.3.1) shows that the formation of compound A (oxycytochrome oxidase) causes no significant infrared absorbance changes at -103 degrees. At -64 degrees, the form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chance B,Leigh JS Jr

更新日期：1977-11-01 00:00:00

abstract：:Novel antibiotics are urgently needed to combat multidrug-resistant pathogens. Venoms represent previously untapped sources of novel drugs. Here we repurposed mastoparan-L, the toxic active principle derived from the venom of the wasp Vespula lewisii, into synthetic antimicrobials. We engineered within its N terminus ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silva ON,Torres MDT,Cao J,Alves ESF,Rodrigues LV,Resende JM,Lião LM,Porto WF,Fensterseifer ICM,Lu TK,Franco OL,de la Fuente-Nunez C

更新日期：2020-10-27 00:00:00

abstract：:The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is a classical nonhomologous end-joining (cNHEJ) factor. Loss of DNA-PKcs diminished mature B cell class switch recombination (CSR) to other isotypes, but not IgG1. Here, we show that expression of the kinase-dead DNA-PKcs (DNA-PKcsKD/KD ) severely compr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crowe JL,Shao Z,Wang XS,Wei PC,Jiang W,Lee BJ,Estes VM,Alt FW,Zha S

更新日期：2018-08-21 00:00:00

abstract：:The fluorescence of the supposedly buried tryptophan in ribonuclease T1 has been found to be collisionally quenched by acrylamide with a rate constant of 3 X 10(8) M--1 sec--1. Only a slight decrease in the quenching rate is observed upon a 5-fold increase in the viscosity of the solution. For this to be the case, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eftink MR,Ghiron CA

更新日期：1975-09-01 00:00:00

abstract：:Endocytic protein trafficking is directed by sorting signals on cargo molecules that are recognized by cytosolic adaptor proteins. However, the steps necessary to segregate the variety of cargoes during endocytosis remain poorly defined. Using Caenorhabditis elegans, we demonstrate that multiple plasma membrane endocy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayers JR,Wang L,Pramanik J,Johnson A,Sarkeshik A,Wang Y,Saengsawang W,Yates JR 3rd,Audhya A

更新日期：2013-07-16 00:00:00

abstract：:ADP activates a family of cell surface receptors that modulate signaling pathways in a broad range of cells. ADP receptor antagonists are widely used to treat cardiovascular disease states. These studies identify a critical role for the stable reactive oxygen species hydrogen peroxide (H2O2) in mediating cellular resp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalwa H,Sartoretto JL,Martinelli R,Romero N,Steinhorn BS,Tao M,Ozaki CK,Carman CV,Michel T

更新日期：2014-03-04 00:00:00

abstract：:Proteins have evolved to be foldable, and yet determinants of foldability may be inapparent once the native state is reached. Insight has emerged from studies of diseases of protein misfolding, exemplified by monogenic diabetes mellitus due to mutations in proinsulin leading to endoplasmic reticulum stress and β-cell ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rege NK,Liu M,Yang Y,Dhayalan B,Wickramasinghe NP,Chen YS,Rahimi L,Guo H,Haataja L,Sun J,Ismail-Beigi F,Phillips NB,Arvan P,Weiss MA

更新日期：2020-11-24 00:00:00

abstract：:In view of the extensive literature on phytochrome mutants in the Ler accession of Arabidopsis, we sought to secure a phytochrome-null line in the same genetic background for comparative studies. Here we report the isolation and phenotypic characterization of phyABCDE quintuple and phyABDE quadruple mutants in the Ler...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu W,Franklin KA,Sharrock RA,Jones MA,Harmer SL,Lagarias JC

更新日期：2013-01-22 00:00:00

abstract：:Biological soil crusts (biocrusts)—communities of mosses, lichens, cyanobacteria, and heterotrophs living at the soil surface—are fundamental components of drylands worldwide, and destruction of biocrusts dramatically alters biogeochemical processes, hydrology, surface energy balance, and vegetation cover. Although th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrenberg S,Reed SC,Belnap J

更新日期：2015-09-29 00:00:00

abstract：:The microbiota is now recognized as a key influence on the host immune response in the central nervous system (CNS). As such, there has been some progress toward therapies that modulate the microbiota with the aim of limiting immune-mediated demyelination, as occurs in multiple sclerosis. However, remyelination-the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMurran CE,Guzman de la Fuente A,Penalva R,Ben Menachem-Zidon O,Dombrowski Y,Falconer J,Gonzalez GA,Zhao C,Krause FN,Young AMH,Griffin JL,Jones CA,Hollins C,Heimesaat MM,Fitzgerald DC,Franklin RJM

更新日期：2019-12-10 00:00:00

abstract：:Bacillus anthracis, the etiologic agent of anthrax, avoids immune surveillance and commandeers host macrophages as a vehicle for lymphatic spreading. Here, we show that B. anthracis edema toxin (ET), via its adenylyl cyclase activity, dramatically increases the motility of infected macrophages and the expression of va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim C,Wilcox-Adelman S,Sano Y,Tang WJ,Collier RJ,Park JM

更新日期：2008-04-22 00:00:00

abstract：:Proceedings of the National Academy of Sciences Colloquium on the roles of homologous recombination in DNA replication are summarized. Current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting DNA replication ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuzminov A

更新日期：2001-07-17 00:00:00

abstract：:Progressive myoclonus epilepsy of Univerricht-Lundborg type is a clinically defined entity among the progressive myoclonus epilepsies. It is an autosomal recessive disorder. The underlying biochemical defect is unknown. We used linkage analysis to localize the gene in 12 families with the aid of polymorphic DNA marker...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehesjoki AE,Koskiniemi M,Sistonen P,Miao J,Hästbacka J,Norio R,de la Chapelle A

更新日期：1991-05-01 00:00:00

abstract：:The configurations of the hydrocarbon chains in micelles are severely constrained by the space-filling requirements of the chain segments, by the continuity of the chains, and by the micellar geometry. A statistical theory that takes full account of these constraints is developed by using a lattice model. The chain en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dill KA,Flory PJ

更新日期：1981-02-01 00:00:00

abstract：:Bone morphogenetic protein 4 (BMP-4) induces ventral mesoderm but represses dorsal mesoderm formation in Xenopus embryos. We show that BMP-4 inhibits two signaling pathways regulating dorsal mesoderm formation, the induction of dorsal mesoderm (Spemann organizer) and the dorsalization of ventral mesoderm. Ectopic expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Re'em-Kalma Y,Lamb T,Frank D

更新日期：1995-12-19 00:00:00

abstract：:microRNA-146a (miR-146a) has been previously implicated as an essential molecular brake, preventing immune overreaction and malignant transformation by attenuating NF-κB signaling, putatively via repression of the Traf6 and Irak1 genes. The exact contribution of miR-146a-mediated silencing of these genes to the contro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Magilnick N,Reyes EY,Wang WL,Vonderfecht SL,Gohda J,Inoue JI,Boldin MP

更新日期：2017-08-22 00:00:00

abstract：:We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 g mL(-1). We observe that the intrins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grover WH,Bryan AK,Diez-Silva M,Suresh S,Higgins JM,Manalis SR

更新日期：2011-07-05 00:00:00

abstract：:Gel-forming mucins, the primary macromolecular components of airway mucus, facilitate airway clearance by mucociliary transport. In cystic fibrosis (CF) altered mucus properties impair mucociliary transport. Airways primarily secrete two closely related gel-forming mucins, MUC5B and MUC5AC. However, their morphologic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ostedgaard LS,Moninger TO,McMenimen JD,Sawin NM,Parker CP,Thornell IM,Powers LS,Gansemer ND,Bouzek DC,Cook DP,Meyerholz DK,Abou Alaiwa MH,Stoltz DA,Welsh MJ

更新日期：2017-06-27 00:00:00

abstract：:Chloroplasts have evolved from a cyanobacterial endosymbiont and been retained for more than 1 billion years by coordinated chloroplast division in multiplying eukaryotic cells. Chloroplast division is performed by ring structures at the division site, encompassing both the inside and the outside of the two envelopes....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyagishima SY,Kuwayama H,Urushihara H,Nakanishi H

更新日期：2008-09-30 00:00:00

abstract：:The Paramyxoviridae family of enveloped viruses enters cells through the concerted action of two viral glycoproteins. The receptor-binding protein, hemagglutinin-neuraminidase (HN), H, or G, binds its cellular receptor and activates the fusion protein, F, which, through an extensive refolding event, brings viral and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bose S,Zokarkar A,Welch BD,Leser GP,Jardetzky TS,Lamb RA

更新日期：2012-09-25 00:00:00

abstract：:The complexity and specificity of metazoan transcription are determined by combinatorial control of the composition and activity of regulatory complexes. To investigate the basis of this specificity, we focused on the glucocorticoid receptor (GR), a single regulatory factor that integrates multiple signals to give ris...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen SH,Masuno K,Cooper SB,Yamamoto KR

更新日期：2013-01-29 00:00:00

abstract：:Breast cancer comprises a heterogeneous set of diseases distinguishable from one another by pathologic presentation and molecular signatures. However, each breast cancer subtype is also heterogeneous. Some of the heterogeneity may be attributable to genetic instability, but recent data emphasize that developmental pla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mizuno H,Spike BT,Wahl GM,Levine AJ

更新日期：2010-12-28 00:00:00