Abstract：
BACKGROUND:It has been demonstrated that cardiac fibroblasts of the human heart have several myocyte-like features, induced by inflammation. OBJECTIVES:This study analyzed the changes of the expressed currents in the basal condition and in the presence of interleukin-6 in cultured human cardiac fibroblasts. METHODS:Human cardiac fibroblasts were cultured as monolayers from earlier passages (2-4). Whole-cell voltage clamp experiments were performed on single culture human cardiac fibroblasts. RESULTS:The cultured human cardiac fibroblasts had a membrane resistance of Rm of 412±91MΩ, and a resting membrane potential of -68.1±3.2mV. Among different cells, we have been analyzed these at which depolarizing clamp steps induced outward currents that reached peak within approx. 20ms and then slowly decayed. Gd3+ decreased the current amplitudes at depolarizing steps. Superfusion with interleukin-6 caused increasing of the outward membrane currents. The changes in the membrane currents continued up to 6min of interleukin-6 perfusion, by reaching their maximum at 3min and slowly decreasing to the level of control recordings at 6min. In the presence of 8μmol/l Gd3+, interleukin-6 does not modify the membrane currents. CONCLUSION:The involvement of mechano sensitive channels in interleukin-6 induced electrical property of fibroblast was proposed. This report presents one particular model of action of interleukin-6, that can open new insights for a deeper understanding of the relationships between interleukin-6 and different ion channels into the fibroblast.
journal_name
Cytokinejournal_title
Cytokineauthors
Mitrokhin V,Kazanski V,Kalsin V,Mladenov M,Kamkin Adoi
10.1016/j.cyto.2017.06.004subject
Has Abstractpub_date
2017-09-01 00:00:00pages
117-122eissn
1043-4666issn
1096-0023pii
S1043-4666(17)30167-9journal_volume
97pub_type
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