Abstract:
AIM:To investigate the cross-talk between oxidative stress and the epidermal growth factor receptor (EGFR)/AKT signaling pathway in retinal pigment epithelial (RPE) cells. METHODS:Human RPE cell lines (ARPE-19 cell) were treated with different doses of epidermal growth factor (EGF) and hydrogen peroxide (H2O2). Cell viability was determined by a methyl thiazolyl tetrazolium assay. Cell proliferation was examined by a bromodeoxyuridine (BrdU) incorporation assay. EGFR/AKT signaling was detected by Western blot. EGFR localization was also detected by immunofluorescence. In addition, EGFR/AKT signaling was intervened upon by EGFR inhibitor (erlotinib), PI3K inhibitor (A66) and AKT inhibitor (MK-2206), respectively. H2O2-induced oxidative stress was blocked by antioxidant N-acetylcysteine (NAC). RESULTS:EGF treatment increased ARPE-19 cell viability and proliferation through inducing phosphorylation of EGFR and AKT. H2O2 inhibited ARPE-19 cell viability and proliferation and also suppressed EGF-stimulated increase of RPE cell viability and proliferation by affecting the EGFR/AKT signaling pathway. EGFR inhibitor erlotinib blocked EGF-induced phosphorylation of EGFR and AKT, while A66 and MK-2206 only blocked EGF-induced phosphorylation of AKT. EGF-induced phosphorylation and endocytosis of EGFR were also affected by H2O2 treatment. In addition, antioxidant NAC attenuated H2O2-induced inhibition of ARPE-19 cell viability through alleviating reduction of EGFR, and phosphorylated and total AKT proteins. CONCLUSION:Oxidative stress affects RPE cell viability and proliferation through interfering with the EGFR/AKT signaling pathway. The EGFR/AKT signaling pathway may be an important target in oxidative stress-induced RPE cell dysfunction.
journal_name
Int J Ophthalmoljournal_title
International journal of ophthalmologyauthors
Chen XD,Su MY,Chen TT,Hong HY,Han AD,Li WSdoi
10.18240/ijo.2017.04.02subject
Has Abstractpub_date
2017-04-18 00:00:00pages
507-514issue
4eissn
2222-3959issn
2227-4898pii
ijo-10-04-507journal_volume
10pub_type
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.09.02
更新日期:2020-09-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2017.05.23
更新日期:2017-05-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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更新日期:2014-08-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2012.05.09
更新日期:2012-01-01 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2016.02.18
更新日期:2016-02-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章,评审
doi:10.18240/ijo.2019.12.17
更新日期:2019-12-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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更新日期:2019-03-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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更新日期:2016-04-18 00:00:00
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pub_type: 杂志文章
doi:10.18240/ijo.2021.01.04
更新日期:2021-01-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.09.17
更新日期:2018-09-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2016.11.18
更新日期:2016-11-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2019.07.19
更新日期:2019-07-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2015.02.01
更新日期:2015-04-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.06.03
更新日期:2018-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2015.03.34
更新日期:2015-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2017.06.11
更新日期:2017-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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abstract::[This retracts the article DOI: 10.3980/j.issn.2222-3959.2015.01.27.]. ...
journal_title:International journal of ophthalmology
pub_type: 撤回出版物
doi:
更新日期:2017-07-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.04.23
更新日期:2018-04-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.03.06
更新日期:2018-03-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2013.03.06
更新日期:2013-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2017.01.12
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2014.05.05
更新日期:2014-10-18 00:00:00
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journal_title:International journal of ophthalmology
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doi:10.18240/ijo.2020.01.18
更新日期:2020-01-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2019.12.08
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.11.20
更新日期:2018-11-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.02.07
更新日期:2018-02-18 00:00:00
abstract:AIM:To observe and characterize imaging features of macular and optic disc areas in less than 60-year-old patients with early primary open angle glaucoma (POAG) by optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA), and to evaluate the diagnostic value of OCT and OCTA. METHODS:Total...
journal_title:International journal of ophthalmology
pub_type: 杂志文章
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更新日期:2020-12-18 00:00:00