Abstract：
AIM:To investigate the cytotoxic effect on human corneal epithelial cells (HCECs) and the ability to faciliate corneal epithelial wound healing of carboxymethylcellulose (CMC) and hyaluronic acid (HA). METHODS:HCECs were exposed to 0.5% CMC (Refresh plus(®), Allergan, Irvine, California, USA) and 0.1% and 0.3% HA (Kynex(®), Alcon, Seoul, Korea, and Hyalein mini(®), Santen, Osaka, Japan) for the period of 30min, and 4, 12, and 24h. Methyl thiazolyl tetrazolium (MTT)-based calorimetric assay was performed to assess the metabolic activity of cellular proliferation and lactate dehydrogenase (LDH) leakage assay to assess the cytotoxicity. Apoptotic response was evaluated with flow cytometric analysis and fluorescence staining with Annexin V and propiodium iodide. Cellular morphology was evaluated by inverted phase-contrast light microscopy and electron microscopy. The wound widths were measured 24h after confluent HCECs were scratch wounded. RESULTS:The inhibitory effect of human corneal epithelial proliferation and cytotoxicity showed the time-dependent response but no significant effect. Apoptosis developed in flow cytometry and apoptotic cells were demonstrated in fluorescent micrograph. The damaged HCECs were detached from the bottom of the dish and showed the well-developed vacuole formations. Both CMC and HA stimulated reepithehlialization of HCECs scratched, which were more observed in CMC. CONCLUSION:CMC and HA, used in artificial tear formulation, could be utilized without any significant toxic effect on HCECs. Both significantly stimulated HCEC reepithelialization of corneal wounds.
journal_name
Int J Ophthalmoljournal_title
International journal of ophthalmologyauthors
Lee JS,Lee SU,Che CY,Lee JEdoi
10.3980/j.issn.2222-3959.2015.02.01subject
Has Abstractpub_date
2015-04-18 00:00:00pages
215-21issue
2eissn
2222-3959issn
2227-4898pii
ijo-08-02-215journal_volume
8pub_type
杂志文章abstract：:A large subset of corneal pathologies involves the formation of new blood and lymph vessels (neovascularization), leading to compromised visual acuity. This article aims to review the clinical causes and presentations of corneal neovascularization (CNV) by examining the mechanisms behind common CNV-related corneal pat...
journal_title：International journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.3980/j.issn.2222-3959.2015.01.32
更新日期：2015-02-18 00:00:00
abstract：AIM:To investigate the relationship between C-reactive protein (CRP) and diabetic retinopathy (DR) in a cohort of Chinese patients with type 2 diabetes mellitus (T2DM). METHODS:Community-based observational cohort study. There were 1131 participants recruited from November 2009 to September 2011 in Desheng community i...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2012.02.07
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abstract：AIM:To investigate the role of heparanase-1 in laser-induced choroidal neovascularization (CNV). METHODS:Experimental CNV was induced by krypton laser photocoagulation in 15 male Brown Norway rats. Fundus fluorescein angiography and histopathological examination were performed in observing the CNV development. The exp...
journal_title：International journal of ophthalmology
pub_type： 杂志文章
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更新日期：2013-04-18 00:00:00
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pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2012.01.06
更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-07-18 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2019.05.16
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2018.08.06
更新日期：2018-08-18 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2016.04.05
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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journal_title：International journal of ophthalmology
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2016-06-18 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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更新日期：2015-06-18 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2012.06.16
更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
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更新日期：2016-02-18 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.18240/ijo.2021.01.14
更新日期：2021-01-18 00:00:00
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journal_title：International journal of ophthalmology
pub_type： 杂志文章
doi：10.3980/j.issn.2222-3959.2015.04.06
更新日期：2015-08-18 00:00:00
