Abstract:
AIM:To investigate the effects of the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 on retinal neovascularization (RNV) in the oxygen-induced retinopathy (OIR) mouse model and human umbilical vein endothelial cells (HUVECs). METHODS:C57BL/6J mice were randomly divided into normoxia-control, OIR-control and LY294002 treatment groups. LY294002 or phosphate-buffered solution was intraperitoneally injected daily into mouse pups from P6 to P9 in LY294002 treatment group or OIR-control group. Morphological and pathological changes in RNV, as well as expression levels of PI3K, serine-threonine kinase (AKT) and vascular endothelial growth factor (VEGF) were observed. HUVECs treating with LY294002 were exposed to hypoxia; the expression of PI3K, AKT and VEGF were examined by Western blot and RT-PCR analyses. RESULTS:Compared with the OIR-control group, LY294002 significantly inhibit RNV. Adenosine diphosphatase (ADPase) staining and hematoxylin and eosin staining indicated that the clock hour scores of neovascularization and the nuclei of pre-retinal neovascular cells in the LY294002 treatment group were clearly less than those in the OIR-control group (1.41±0.52 vs 6.20±1.21; 10.50±1.58 vs 22.25±1.82, both P<0.05). Intravitreal injection of LY294002 (in the LY294002 treatment group) markedly decreased PI3K/AKT-VEGF expression compared with the OIR-control group by immunohistochemistry, Western blotting and RT-PCR (all P<0.05). In HUVECs treated with hypoxia, expression of PI3K, AKT and VEGF were downregulated in the hypoxia-LY294002 group (all P<0.05). CONCLUSION:The PI3K inhibitor LY294002 can inhibit RNV by downregulating PI3K, AKT, and VEGF expression in vivo and in vitro. LY294002 may provide an effective method for preventing retinopathy of prematurity (ROP).
journal_name
Int J Ophthalmoljournal_title
International journal of ophthalmologyauthors
Di Y,Chen XLdoi
10.18240/ijo.2018.08.06subject
Has Abstractpub_date
2018-08-18 00:00:00pages
1284-1289issue
8eissn
2222-3959issn
2227-4898pii
ijo-11-08-1284journal_volume
11pub_type
杂志文章abstract:AIM:To investigate the efficacy and safety of the treatment of endoscopic trans-ethmosphenoid optic canal decompression (ETOCD) with combination of steroid in patients with newly onset indirect traumatic optic neuropathy (ITON) and compare the outcome between immediate ETOCD treatment and ETOCD with preoperative steroi...
journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.01.18
更新日期:2020-01-18 00:00:00
abstract::Phacoemulsification is the main gold standard for cataract operation in the developed world together with foldable intraocular lens (IOL) implantation by injection, allowing for stable wound construction and less postoperative astigmatism. It is a safe procedure with high success rate with the advancement in machines,...
journal_title:International journal of ophthalmology
pub_type: 杂志文章
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2011.04.26
更新日期:2011-01-01 00:00:00
abstract::We report a case of syndromic gingival fibromatosis with notable ocular lesions, bilateral congenital cataracts, esotropia, and high myopia of a 21-year-old male patient from China. The patient was diagnosed with gingival fibromatosis based on his massive gingival overgrowth and histological findings that were consist...
journal_title:International journal of ophthalmology
pub_type: 杂志文章
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更新日期:2014-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2017.09.22
更新日期:2017-09-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2010.02.03
更新日期:2010-01-01 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.04.03
更新日期:2018-04-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.09.07
更新日期:2018-09-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2017.07.10
更新日期:2017-07-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2012.02.07
更新日期:2012-01-01 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2019.01.08
更新日期:2019-01-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2016.05.06
更新日期:2016-05-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2015.02.01
更新日期:2015-04-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.02.14
更新日期:2020-02-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章,评审
doi:10.18240/ijo.2019.10.16
更新日期:2019-10-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.11.15
更新日期:2020-11-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.08.26
更新日期:2018-08-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.08.02
更新日期:2020-08-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.04.10
更新日期:2020-04-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2019.06.10
更新日期:2019-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2015.04.25
更新日期:2015-08-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2020.01.08
更新日期:2020-01-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
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更新日期:2016-03-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2011.03.02
更新日期:2011-01-01 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.07.01
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2013.05.06
更新日期:2013-10-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.18240/ijo.2018.06.03
更新日期:2018-06-18 00:00:00
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journal_title:International journal of ophthalmology
pub_type: 杂志文章
doi:10.3980/j.issn.2222-3959.2015.06.09
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