当前位置： SCI文献检索 > International Journal of Ophthalmology期刊下所有文献 > Effect of senescence marker protein 30 on the proliferation and apoptosis of human lens epithelial cells SRA01/04.

Effect of senescence marker protein 30 on the proliferation and apoptosis of human lens epithelial cells SRA01/04.

Abstract：

AIM:To study the effect of senescence marker protein 30 (SMP30) on the proliferation and apoptosis of human lens epithelial cell (HLEC) SRA01/04. METHODS:SMP30 overexpression (OE) and knock down (KD) type cell lines were cultivated by using two groups regucalcin (RGN; SMP30) lentiviral vectors (LV-RGN, LV-RGN-RNAi) and the respective negative control virus infect SRA01/04 cells. Western blot and real-time quantitative polymerase chain reaction (q-PCR) analysis were used to determine RGN overexpression and knock down efficiency. We use cell counting kit-8 (CCK8) assay to measure cell viability and 5-bromodeoxyuridine (BrdU) assay to test cell proliferation. Cell cycle was measured by PI FACS assay and cell apoptosis was tested by Annexin V-APC assay through flow cytometry. We use Western blot to measure the content of caspase-3 in SRA01/04. RESULTS:We used PCR and Western blot techniques to determine the successful transfection of SMP30 OE and KD SRA01/04 cell lines. By CCK8, Brdu and PI FACS cell cycle assay, it was found that the SMP30 OE group promoted cell proliferation (P<0.05) compared with the control group, and the KD group inhibited cell proliferation (P<0.05). The results of Annexin V-APC signal staining detection indicated that compared with respective control group, the cell apoptosis rate was higher in KD group (P<0.05) but lower in OE group (P<0.01). The expression of caspase-3 was down-regulated in OE group through Western blot assay and up-regulated in KD group compared with respective control group. CONCLUSION:Proliferation of SRA01/04 was promoted by SMP30 OE and apoptosis was suppressed. Increasing the expression of SMP30 may protect HLEC SRA01/04 against apoptosis in cataract.

journal_name

Int J Ophthalmol

journal_title

International journal of ophthalmology

authors

Chen X,Li SM,Li YW,Han ZH,Liang H

doi

10.18240/ijo.2018.04.03

subject

Has Abstract

pub_date

2018-04-18 00:00:00

pages

553-558

issue

4

eissn

2222-3959

issn

2227-4898

pii

ijo-11-04-553

journal_volume

11

pub_type

杂志文章

相关文献

International Journal of Ophthalmology文献大全
  • Measurements of anterior segment parameters using three different non-contact optical devices in keratoconus patients.

    abstract：AIM:To evaluate the measurements of anterior segment parameters using three different non-contact optical devices in keratoconus patients. METHODS:A hundred and one eyes of 55 keratoconus patients were enrolled in this study. The mean age was 26.2±8.9 years. The inclusion criteria were keratoconus stage I to III accor...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2013.04.21

    authors： Yazıcı AT,Pekel G,Bozkurt E,Yıldırım Y,Pekel E,Demirok A,Yılmaz OF

    更新日期：2013-08-18 00:00:00

  • Effect of lens constants optimization on the accuracy of intraocular lens power calculation formulas for highly myopic eyes.

    abstract：AIM:To evaluate the effect of different lens constant optimization methods on the accuracy of intraocular lens (IOL) power calculation formulas for highly myopic eyes. METHODS:This study comprised 108 eyes of 94 consecutive patients with axial length (AL) over 26 mm undergoing phacoemulsification and implantation of a...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2019.06.10

    authors： Zhang JQ,Zou XY,Zheng DY,Chen WR,Sun A,Luo LX

    更新日期：2019-06-18 00:00:00

  • Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.

    abstract：AIM:To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS:We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interac...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2013.03.06

    authors： Yan LB,Shi K,Bing ZT,Sun YL,Shen Y

    更新日期：2013-06-18 00:00:00

  • Comparative analysis of the efficacy of astigmatic correction after wavefront-guided and wavefront-optimized LASIK in low and moderate myopic eyes.

    abstract：AIM:To evaluate and compare the efficacy of the astigmatic correction achieved with laser in situ keratomileusis (LASIK) in eyes with myopic astigmatism using wavefront-guided (WFG) and wavefront-optimized (WFO) ablation profiles. METHODS:Prospective study included 221 eyes undergoing LASIK: 99 and 122 eyes with low a...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2017.02.17

    authors： Khalifa MA,Alsahn MF,Shaheen MS,Pinero DP

    更新日期：2017-02-18 00:00:00

  • Comparative study of visual acuity and aberrations after intralase femtosecond LASIK: small corneal flap versus big corneal flap.

    abstract：AIM:To study the effect of different flap sizes on visual acuity, refractive outcomes, and aberrations after femtosecond laser for laser in situ keratomileusis (LASIK). METHODS:In each of the forty patients enrolled, 1 eye was randomly assigned to receive treatment with a 8.1mm diameter corneal flap, defined as the sm...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2013.05.16

    authors： Zhang YL,Liu L,Cui CX,Hu M,Li ZN,Cao LJ,Jing XH,Mu GY

    更新日期：2013-10-18 00:00:00

  • Identification of the preferentially targeted proteins by carbamylation during whole lens incubation by using radio-labelled potassium cyanate and mass spectrometry.

    abstract：AIM:To attempt to identify the primary targets of carbamylation in bovine lenses incubated under physiological condition. METHODS:Fresh intact bovine lenses were incubated with [(14)C]-labelled potassium cyanate for seven days. The water-soluble proteins (WSP) of both cortex and nucleus lens were isolated by size-excl...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2010.02.03

    authors： Yan H,Zhang J,Harding JJ

    更新日期：2010-01-01 00:00:00

  • Optic disc and peripapillary changes by optic coherence tomography in high myopia.

    abstract：:Myopia, a worldwide condition, is a multifactorial disease resulting in many ocular complications. Early onset of myopia has a great tendency to develop high myopia and pathological myopia later in life. The pathophysiology and progression of myopia is still unclear. Owing to its involving in visual function, optic di...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章,评审

    doi：10.18240/ijo.2018.05.25

    authors： Pan T,Su Y,Yuan ST,Lu HC,Hu ZZ,Liu QH

    更新日期：2018-05-18 00:00:00

  • 5-year follow-up of combined non-topography guided photorefractive keratectomy and corneal collagen cross linking for keratoconus.

    abstract：AIM:To evaluate the visual outcomes of simultaneous non-topography guided photorefractive keratectomy (PRK) and corneal collagen cross-linking (CXL) in eyes with keratoconus 5y after the procedure. METHODS:Prospective, interventional, non-randomized, and non-controlled case series design was used. Sixty eyes of 30 pat...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2018.01.09

    authors： Al-Amri AM

    更新日期：2018-01-18 00:00:00

  • Modified ulcer debridement in the treatment of the superficial fungal infection of the cornea.

    abstract：AIM:To evaluate the efficacy of modified corneal ulcer debridement in superficial fungal keratitis unresponsive to medications. METHODS:A total of 209 patients (209 eyes) with fungal keratitis, involving no more than 50% of the stromal depth and not responding to antifungal agents for 2wk, were recruited in this retro...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2018.02.07

    authors： Wang JY,Wang DQ,Qi XL,Cheng J,Xie LX

    更新日期：2018-02-18 00:00:00

  • Surgical treatment and visual outcomes of cataract with persistent hyperplastic primary vitreous.

    abstract：AIM:To evaluate the surgical treatment and visual outcomes of eyes with cataract and persistent hyperplastic primary vitreous (PHPV). METHODS:This retrospective study included patients with cataract and PHPV treated with various strategies. Anterior PHPV was treated using phacoemulsification with underwater electric c...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2017.03.11

    authors： Li L,Fan DB,Zhao YT,Li Y,Cai FF,Zheng GY

    更新日期：2017-03-18 00:00:00

  • Correlation between optical coherence tomography, multifocal electroretinogram findings and visual acuity in diabetic macular edema.

    abstract：AIM:To analyze the correlation between macular morphology and function in eyes with diabetic macular edema (DME). METHODS:Fifty-five eyes with different visual acuity (VA) of 32 patients who suffered from DME were analyzed using multifocal electroretinography (mfERG) and optical coherence tomography (OCT). The paramet...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2020.10.13

    authors： Xia HH,Chen JL,Chen HY,Lin HJ

    更新日期：2020-10-18 00:00:00

  • Changed trends of major causes of visual impairment in Sichuan, China from 1987 to 2006.

    abstract：AIM:To study the trends of major causes of visual impairment (VI) in adults in Sichuan, China and evaluate the effect of aging on the trends. METHODS:We used data from the National Sample Survey on Disabilities (NSSD) in Sichuan province conducted in 1987 and 2006. The age-adjusted prevalence of major causes of VI and...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2014.01.26

    authors： Chen H,Fan YC,He QH,Wu XY,Wei M,Eichner JE,Farris BK,Hildebrand PL,Lei CT,Wu SH,Yang JY

    更新日期：2014-02-18 00:00:00

  • Corneal topographic changes and surgically induced astigmatism following combined phacoemulsification and 25-gauge vitrectomy.

    abstract：AIM:To evaluate corneal topographic changes and surgically induced astigmatism (SIA) after combined phacoemulsification and 25-gauge transconjunctival sutureless vitrectomy (25-G TSV). METHODS:A retrospective study on 96 eyes of 87 patients who underwent combined phacoemulsification and 25-G TSV. The different topogra...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2017.01.12

    authors： Sayed KM,Farouk MM,Katome T,Nagasawa T,Naito T,Mitamura Y

    更新日期：2017-01-18 00:00:00

  • Comparison of posterior capsule opacification at 360-degree square edge hydrophilic and sharp edge hydrophobic acrylic intraocular lens in diabetic patients.

    abstract：AIM:To compare posterior capsule opacification (PCO) degree and visual functions after phacoemulsification in eyes implanted with 360-degree square edge hydrophilic acrylic intraocular lens (IOL) (570C C-flex, Rayner) and sharp edge hydrophobic acrylic IOL (Sensar AR40e, AMO) in diabetic patients. METHODS:Sixty diabet...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2015.04.15

    authors： Bai L,Zhang J,Chen L,Ma T,Liang HC

    更新日期：2015-08-18 00:00:00

  • Comparison of clinical outcomes of deep anterior lamellar keratoplasty and excimer laser assisted anterior lamellar keratoplasty in keratoconus.

    abstract：AIM:To compare and evaluate the clinical outcomes of deep anterior lamellar keratoplasty (DALK) and excimer laser assisted anterior lamellar keratoplasty (ELLK) in eyes with keratoconus. METHODS:In this study, 57 eyes of 56 patients operated between 2013 to 2017 were included. Thirty-one eyes underwent big-bubble DALK...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2019.04.09

    authors： Ceylanoglu KS,Aydin B,Ozmen MC

    更新日期：2019-04-18 00:00:00

  • Retraction Notice.

    abstract：:[This retracts the article DOI: 10.3980/j.issn.2222-3959.2015.01.27.]. ...

    journal_title：International journal of ophthalmology

    pub_type： 撤回出版物

    doi：

    authors：

    更新日期：2017-07-18 00:00:00

  • Posterior vitreous detachment rate following intravitreal dexamethasone injection.

    abstract：AIM:To determine whether intravitreal dexamethasone (DEX) implant induces posterior vitreous detachment or not. METHODS:We retrospectively reviewed 810 eyes of 405 patients who underwent intravitreal DEX implantation due to macular edema caused by diabetic and retinal venous occlusion in our clinic. The eyes having no...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2019.08.10

    authors： Alpay A

    更新日期：2019-08-18 00:00:00

  • Comparison of one and three initial monthly intravitreal ranibizumab injection in patients with macular edema secondary to branch retinal vein occlusion.

    abstract：AIM:To compare three initial monthly intravitreal ranibizumab (IVR) injections followed by pro re nata (PRN) dosing with one initial monthly IVR injections followed by PRN dosing for macular edema (ME) secondary to branch retinal vein occlusion (BRVO). METHODS:Forty-two eyes of 42 patients who had IVR injections for B...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2018.09.17

    authors： Bayat AH,Çakır A,Özturan ŞG,Bölükbaşı S,Erden B,Elçioğlu MN

    更新日期：2018-09-18 00:00:00

  • TNF related apoptosis-inducing ligand and its receptors in ocular tumors.

    abstract：:Most of the ocular tumors have poor prognosis, and they remain a difficult problem in the area of ophthalmology. With the rapid development of molecular biology and immunologic techniques and the deep research on ocular tumor related genes, it becomes possible to diagnose and treat malignant tumors from the molecular ...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2011.05.18

    authors： Ning Q,Hou L,Meng M,Pan BR,Zhao XH

    更新日期：2011-01-01 00:00:00

  • Combine intravitreal bevacizumab with Nd: YAG laser hyaloidotomy for valsalva pre-macular haemorrhage and observe the internal limiting membrane changes: a spectralis study.

    abstract：:Valsalva retinopathy was described as a particular form of retinopathy, pre-retinal and subinternal limiting membrane haemorrhages in nature that rarely may break through and become subhyloid or intravitreal, secondary to a sudden increase in intrathoracic pressure. We reported a new way that Nd:YAG laser for ILM hyal...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2013.02.25

    authors： Hua R,Liu LM,Hu YD,Zhou Y,Chen L

    更新日期：2013-04-18 00:00:00

  • The thickness and volume of the choroid, outer retinal layers and retinal pigment epithelium layer changes in patients with diabetic retinopathy.

    abstract：AIM:To evaluate the thickness and volume changes of the choroidal, outer retinal layers (ORL) and retinal pigment epithelium (RPE) in patients with diabetic retinopathy (DR) using optical coherence tomography (OCT) and correlate them with visual acuity. METHODS:We carried out a retrospective observational case series....

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2018.12.14

    authors： Wang XN,Li ST,Li W,Hua YJ,Wu Q

    更新日期：2018-12-18 00:00:00

  • Comparison of the impact of nesofilcon A hydrogel contact lens on the ocular surface and the comfort of presbyopic and non-presbyopic wearers.

    abstract：AIM:To assess and compare the impact of a daily disposable contact lens (CL) with high water content on the ocular surface and comfort of the presbyopic and non-presbyopic population after one day of use. METHODS:Totally 20 presbyopes and 30 non-presbyopes non-contact wearers were fitted with nesofilcon A CLs. CL thic...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2019.04.19

    authors： Lorente-Velázquez A,García-Montero M,Gómez-Sanz FJ,Rico Del Viejo L,Hernández-Verdejo JL,Madrid-Costa D

    更新日期：2019-04-18 00:00:00

  • Role of IgG4 serology in identifying common orbital lymphoproliferative disorders.

    abstract：AIM:To explore the role of IgG4 serology in identifying common orbital lymphoproliferative disorders. METHODS:Eighty-one patients with orbital lymphoproliferative diseases were treated in the Department of Ocular Oncology, Beijing Tongren Hospital, Capital Medical University between September 2010 and December 2012. S...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.18240/ijo.2016.02.18

    authors： Li J,Ma JM,Ge X

    更新日期：2016-02-18 00:00:00

  • Expression of dectin-1 during fungus infection in human corneal epithelial cells.

    abstract：AIM:To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus. METHODS:A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real-time qPCR and immuno...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2014.01.06

    authors： Li C,Zhao GQ,Che CY,Li N,Lin J,Xu Q,Wang Q,Liu Y,Qiu S

    更新日期：2014-02-18 00:00:00

  • Comparison of intravitreal bevacizumab with intravitreal triamcinolone acetonide for treatment of cystoid macular edema secondary to retinal vein occlusion: a Meta-analysis.

    abstract：AIM:To compare the effects of intravitreal injection of bevacizumab (IVB) with intravitreal triamcinolone acetonide (IVTA) on the treatment of cystoid macular edema (CME) secondary to retinal vein occlusion (RVO). METHODS:A literature search was conducted using PubMed, the Cochrane Central Register of Controlled Trial...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2015.06.29

    authors： Sun Y,Qu Y

    更新日期：2015-12-18 00:00:00

  • Evaluation of choroidal thickness using spectral-domain optical coherence tomography in patients with severe obstructive sleep apnea syndrome: a comparative study.

    abstract：AIM:To assess choroidal thickness in patients with severe obstructive sleep apnea syndrome (OSAS) and compare them with healthy controls, using spectral domain optical coherence tomography (OCT). METHODS:In this observational, cross-sectional study, choroidal thicknesses of 23 newly severe OSAS patients and 23 body ma...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2014.06.22

    authors： Karalezli A,Eroglu FC,Kivanc T,Dogan R

    更新日期：2014-12-18 00:00:00

  • Aerocyst urethral catheter insertion compared to expansion sponges application in external dacryocystorhinostomy.

    abstract：AIM:To evaluate the clinical effect and complications of two different filling materials (aerocyst urethral catheter and expansion sponges) applying in external dacryocystorhinostomy (EXT-DCR) and compare their advantages and disadvantages. METHODS:A retrospective study was made in the period from April, 1 2000 to Apr...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2011.05.08

    authors： Shao Y,Yu Y,Pei CG,Zhou Q,Dong WJ,Qu Y,Yang L,Gao GP

    更新日期：2011-01-01 00:00:00

  • Aberration changes of the corneal anterior surface following discontinued use of rigid gas permeable contact lenses.

    abstract：AIM:To record aberrations with a corneal topographic device on the anterior surface of the cornea at different time-points prior to wearing and following discontinued use of rigid gas permeable (RGP) contact lenses. The effect of wearing RGP on the anterior surface of the cornea was discussed to provide guidance for cl...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2013.02.14

    authors： Yu Q,Wu JX,Zhang HN,Ye S,Dong SQ,Zhang CH

    更新日期：2013-04-18 00:00:00

  • Immediate intraocular pressure rise after intravitreal injection of ranibizumab and two doses of triamcinolone acetonide.

    abstract：AIM:To evaluate prospectively immediate intraocular pressure (IOP) changes after the intravitreal injection of ranibizumab, 2 and 4mg triamcinolone acetonide. METHODS:Patients who underwent intravitreal injection of 0.1mL (4mg) triamcinolone acetonide (TA, Group T4), 0.05mL (2mg) TA (Group T2) and 0.05mL (0.5mg) ranib...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2011.04.16

    authors： Arikan G,Osman Saatci A,Hakan Oner F

    更新日期：2011-01-01 00:00:00

  • Effect of Bak Foong Pills on the expression of β-amyloid in rat retina with optic nerve transection.

    abstract：AIM:To investigate the effect of Bak Foong Pills (BFP) on the expression of β-amyloid (Aβ) in rats retina with optic nerve transection, and its roles and possible mechanisms in protecting optic nerve damage. METHODS:Seventy-two healthy, Sprague-Dawley, adult rats were randomly assigned to three groups: negative contro...

    journal_title：International journal of ophthalmology

    pub_type： 杂志文章

    doi：10.3980/j.issn.2222-3959.2011.01.13

    authors： Zhao TT,Li YQ,Tang LS,Li YH,Fan F,Guo XJ

    更新日期：2011-01-01 00:00:00