Expression of dectin-1 during fungus infection in human corneal epithelial cells.

Abstract:

AIM:To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus. METHODS:A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus (AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0, 4, 8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately. RESULTS:Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h, and dectin-1 protein expression increased after stimulation at 24h. CONCLUSION:Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro.

journal_name

Int J Ophthalmol

authors

Li C,Zhao GQ,Che CY,Li N,Lin J,Xu Q,Wang Q,Liu Y,Qiu S

doi

10.3980/j.issn.2222-3959.2014.01.06

subject

Has Abstract

pub_date

2014-02-18 00:00:00

pages

34-7

issue

1

eissn

2222-3959

issn

2227-4898

pii

ijo-07-01-034

journal_volume

7

pub_type

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