Suppressive effect of microRNA-143 in retinoblastoma.

Abstract:

AIM:To investigate microRNA-143 expression and effect on suppression of retinoblastoma (RB) cells. METHODS:The expression of microRNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1. The microRNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods. RESULTS:The microRNA-143 expression was significantly suppressed in RB cell lines. Overexpression of microRNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the microRNA-143 mimics transfected group than that in the negative control and the microRNA-143 inhibitor groups. CONCLUSION:MicroRNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.

journal_name

Int J Ophthalmol

authors

Wang LL,Hu HF,Feng YQ

doi

10.18240/ijo.2016.11.08

subject

Has Abstract

pub_date

2016-11-18 00:00:00

pages

1584-1590

issue

11

eissn

2222-3959

issn

2227-4898

pii

ijo-09-11-1584

journal_volume

9

pub_type

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