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Comparison of lens oxidative damage induced by vitrectomy and/or hyperoxia in rabbits.

Abstract：

AIM:To compare of lens oxidative damage induced by vitrectomy and/or hyperoxia in rabbit. METHODS:Sixteen New Zealand rabbits (2.4-2.5 kg) were randomly divided into two groups (Group A, n=12; Group B, n=4). In Group A, the right eyes were treated with vitrectomy and systemic hyperoxia (oxygen concentration: 80%-85%, 1 ATA, 4h/d) (Group A-right), and the left eyes were treated with hyperoxia without vitrectomy surgery (Group A-left). Four rabbits in group B (eight eyes) were untreated as the controls. Lens transparency was monitored with a slit lamp and recorded before and after vitrectomy. After hyperoxic treatment for 6mo, the eyeballs were removed and the lens cortices (containing the capsules) and nuclei were separated for further morphological and biochemical evaluation. RESULTS:Six months after treatments, there were no significant morphological changes in the lenses in any experimental group when observed with a slit lamp. However, the levels of water-soluble proteins and ascorbate, and the activities of catalase and Na+-K+-ATPase were significantly reduced, whereas the levels of malondialdehyde and transforming growth factor β2 (TGF-β2) were significantly elevated, in both the cortices and nuclei of eyes treated with vitrectomy and hyperoxia. The increase in protein-glutathione mixed disulfides and the reduction in water-soluble proteins were more obvious in the lens nuclei. The levels of ascorbate in the vitreous fluid were also reduced after vitrectomy, whereas TGF-β2 increased after vitrectomy and hyperoxia. Systemic hyperoxia exposure increased these effects. CONCLUSION:Removal of the intact vitreous gel with vitrectomy and exposing the lens to increased oxygen from the retina induce lens oxidation and aggregation. Thus, an intact vitreous gel structure may protect the lens from oxidative insult and maintain lens transparency.

journal_name

Int J Ophthalmol

journal_title

International journal of ophthalmology

authors

Yan H,Wang D,Ding TB,Zhou HY,Yan WJ,Wang XC

doi

10.18240/ijo.2017.01.02

subject

Has Abstract

pub_date

2017-01-18 00:00:00

pages

6-14

issue

eissn

2222-3959

issn

2227-4898

pii

ijo-10-01-006

journal_volume

pub_type

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Comparison of lens oxidative damage induced by vitrectomy and/or hyperoxia in rabbits.