Changes of matrix metalloproteinases in the stroma after corneal cross-linking in rabbits.

Abstract:

AIM:To observe changes in the content of matrix metalloproteinases (MMPs) in the corneal stroma after corneal cross-linking (CXL) in rabbits, and further explore the corneal pathophysiological process after CXL. METHODS:Forty-two rabbits (42 eyes) were randomly divided into seven groups. One group served as the control group, while the other six groups were treated with CXL. The concentrations of MMPs in corneal stroma were evaluated through parallel reaction monitoring at baseline and 3, 7, 15, 30, 90, and 180d after treatment. RESULTS:The levels of MMP-2 in the corneal stroma of rabbits were 0.76±0.07, 2.78±1.39, 4.12±0.69, 2.00±0.29, 2.00±0.30, 1.22±0.18, and 1.35±0.18 (10-9 mol/g) at baseline and 3, 7, 15, 30, 90, and 180d after treatment, respectively. The contents of tissue inhibitor of metalloproteinase-1 (TIMP-1) were 1.83±0.26, 7.94±0.58, 6.95±2.64, 3.81±0.48, 3.07±0.92, 1.72±0.19, and 1.69±0.74 (10-9 mol/g), respectively. The ratios of MMP-2/TIMP-1 were 0.42±0.33, 0.36±0.20, 0.62±0.10, 0.54±0.15, 0.68±0.13, 0.71±0.10, and 0.68±0.09, respectively. After CXL, the expression of MMP-2 and TIMP-1 in the rabbit corneal stroma was initially increased and subsequently decreased. The levels of MMP-2 remained higher than those recorded at baseline 180d after treatment, but it was not statistically significant. The levels of TIMP-1 returned to baseline levels at 90d after treatment. The ratio of MMP-2/TIMP-1 started to rise from 7d after CXL. It was significantly higher than that calculated at baseline 30-180d after CXL. The results for MMP-1, -3, -7, -9, -13, and TIMP-2 were negative. CONCLUSION:CXL can lead to changes in the content of MMP-2 and TIMP-1 in the rabbit corneal stroma. The ratio of MMP-2/TIMP-1 remains higher versus baseline, indicating that MMP-2 is involved in the corneal pathophysiological process after CXL.

journal_name

Int J Ophthalmol

authors

Jia HZ,Pang X,Peng XJ

doi

10.18240/ijo.2021.01.04

subject

Has Abstract

pub_date

2021-01-18 00:00:00

pages

26-31

issue

1

eissn

2222-3959

issn

2227-4898

pii

ijo-14-01-026

journal_volume

14

pub_type

杂志文章
  • Clinical and laboratory features of PCR-confirmed periocular tuberculosis in China.

    abstract::Experts lack knowledge of periocular tuberculosis (TB) in China. Nested polymerase chain reaction (PCR) shows advantages in diagnosis of extrapulmonary TB. Our study aims to explore the clinical and laboratory features of PCR-confirmed periocular TB. We retrospectively reviewed medical records of presumptive periocula...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.11.20

    authors: Ma RQ,Gan L,Bi YW,Yuan YF,Ren H,Qian J

    更新日期:2018-11-18 00:00:00

  • Toxocara optic neuropathy: clinical features and ocular findings.

    abstract::We evaluated thirteen eyes of twelve patients diagnosed clinically and serologically with Toxocara optic neuropathy. Eleven patients had unilateral involvement and one patient had bilateral optic neuropathy. Eight patients (66.7%) had a possible infection source to Toxocara. Six patients (50%) had painless acute optic...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.03.26

    authors: Choi KD,Choi JH,Choi SY,Jung JH

    更新日期:2018-03-18 00:00:00

  • Posterior vitreous detachment rate following intravitreal dexamethasone injection.

    abstract:AIM:To determine whether intravitreal dexamethasone (DEX) implant induces posterior vitreous detachment or not. METHODS:We retrospectively reviewed 810 eyes of 405 patients who underwent intravitreal DEX implantation due to macular edema caused by diabetic and retinal venous occlusion in our clinic. The eyes having no...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2019.08.10

    authors: Alpay A

    更新日期:2019-08-18 00:00:00

  • Imaging characteristics and surgical management of orbital neurilemmomas.

    abstract:AIM:To review imaging characteristics and surgical outcomes of orbital neurilemmoma. METHODS:Retrospective review of 21 patients with orbital neurilemmoma managed at the Zhongshan Ophthalmic Center of Sun Yat-sen University from June 2005 to December 2016. All patients underwent surgical excision following preoperativ...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2019.07.09

    authors: Chen MH,Yan JH

    更新日期:2019-07-18 00:00:00

  • Identification of differentially expressed metastatic genes and their signatures to predict the overall survival of uveal melanoma patients by bioinformatics analysis.

    abstract:AIM:To identify metastatic genes and miRNAs and to investigate the metastatic mechanism of uveal melanoma (UVM). METHODS:GSE27831, GSE39717, and GSE73652 gene expression profiles were downloaded from the Gene Expression Omnibus (GEO) database, and the limma R package was used to identify differentially expressed genes...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2020.07.05

    authors: Zhao DD,Zhao X,Li WT

    更新日期:2020-07-18 00:00:00

  • Effect of brain-derived neurotrophic factor on c-jun expression in the rd mouse retina.

    abstract:AIM:To determine the location of c-jun protein, dynamic changes in c-jun mRNA and protein expression, and ultrastructure characteristics in the rd mouse retina, following a single dose of brain-derived neurotrophic factor (BDNF) in a short period of time. METHODS:A single intravitreal injection of BDNF at two dosages ...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2012.03.03

    authors: Chen R,Yin XB,Peng CX,Li GL

    更新日期:2012-01-01 00:00:00

  • Comparison of one and three initial monthly intravitreal ranibizumab injection in patients with macular edema secondary to branch retinal vein occlusion.

    abstract:AIM:To compare three initial monthly intravitreal ranibizumab (IVR) injections followed by pro re nata (PRN) dosing with one initial monthly IVR injections followed by PRN dosing for macular edema (ME) secondary to branch retinal vein occlusion (BRVO). METHODS:Forty-two eyes of 42 patients who had IVR injections for B...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.09.17

    authors: Bayat AH,Çakır A,Özturan ŞG,Bölükbaşı S,Erden B,Elçioğlu MN

    更新日期:2018-09-18 00:00:00

  • Effect of hypoxia on the proliferation of murine cornea limbal epithelial progenitor cells in vitro.

    abstract:AIM:To investigate the effect of hypoxia on the proliferation of mouse corneal epithelial cells in vitro. METHODS:Mouse corneal epithelial cells(MCEs) were cultured in normoxia (210mL/L O(2) and 50mL/L CO(2)) and hypoxia (20mL/L O(2) and 50mL/L CO(2)), respectively. Colony forming efficiency (CFE) and cell proliferati...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2011.02.07

    authors: Ma XL,Liu HQ

    更新日期:2011-01-01 00:00:00

  • Non-buckled vitrectomy for retinal detachment with inferior breaks and proliferative vitreoretinophathy.

    abstract:AIM:To investigate the efficacy of non-buckled vitrectomy with classical endotamponade agents in the treatment of primary retinal detachment (RD) complicated by inferior breaks and proliferative vitreoretinophathy (PVR). METHODS:A retrospective, consecutive and case series study of 40 patients with inferior break RD a...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2012.05.09

    authors: Sheng Y,Sun W,Mo B,Yu YJ,Gu YS,Liu W

    更新日期:2012-01-01 00:00:00

  • The role of intravitreal ranubizumab in the treatment of familial exudative vitreoretinopathy of stage 2 or greater.

    abstract:AIM:To evaluate the role of intravitreal ranubizumab (IVR) in the treatment of familial exudative vitreoretinopathy (FEVR) of stage 2 or greater either as primary or an ajunct to conventional treatments. METHODS:Retrospective, non-controlled clinical study. Thirty patients (37 eyes) diagnosed with FEVR were enrolled. ...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.06.13

    authors: Lu YZ,Deng GD,Liu JH,Yan H

    更新日期:2018-06-18 00:00:00

  • Curative effect assessment of bandage contact lens in neurogenic keratitis.

    abstract:AIM:To observe the curative effect of bandage contact lens in neurogenic keratitis. METHODS:Twenty cases of neurogenic keratitis were studied at the Department of Ophthalmology, the first Affiliated Hospital of China Medical University, between October 2012 and June 2013. These included 13 males and 7 females, aged fr...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2014.06.12

    authors: Sun YZ,Guo L,Zhang FS

    更新日期:2014-12-18 00:00:00

  • Effects of resveratrol on ARPE-19 cell proliferation and migration via regulating the expression of proliferating cell nuclear antigen, P21, P27 and p38MAPK/MMP-9.

    abstract:AIM:To explore whether resveratrol (Res) can inhibit human retinal pigment epithelial cell (ARPE-19 cell) proliferation and migration, and to research the molecular mechanisms. METHODS:ARPE-19 cells were pretreated with various concentrations at 0, 50, 100, 150, 200 and 300 µmol/L of Res, and with 0 µmol/L Res as the ...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2016.12.04

    authors: Hao XN,Wang WJ,Chen J,Zhou Q,Qu YX,Liu XY,Xu W

    更新日期:2016-12-18 00:00:00

  • Risk factors for endophthalmitis after cataract surgery in diabetic patients: a case control study.

    abstract:AIM:To identify risk factors associated with post-cataract surgery endophthalmitis (PCE) in type 2 diabetic patients. METHODS:A hospital-based retrospective case-control study was conducted on 194 type 2 diabetic patients undergoing cataract surgery in Rajavithi Hospital from January 2007 to December 2015. Fifteen pat...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2019.03.11

    authors: Silpa-Archa S,Papirachnart A,Singhanetr P,Preble JM

    更新日期:2019-03-18 00:00:00

  • Risk factors for endophthalmitis following cataract surgery-our experience at a tertiary eye care centre in India.

    abstract:AIM:To determine the risk factors for acute endophthalmitis after cataract extraction in a tertiary care centre in India. METHODS:We performed a nested case control study within a retrospective cohort. The surgical records of all patients with clinically diagnosed endophthalmitis within one month after cataract surger...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2015.06.19

    authors: Khanna RC,Ray VP,Latha M,Cassard SD,Mathai A,Sekhar GC

    更新日期:2015-12-18 00:00:00

  • Association of macular pigment optical density with early stage of non-proliferative diabetic retinopathy in Chinese patients with type 2 diabetes mellitus.

    abstract:AIM:To detect the association between macular pigment optical density (MPOD), which reflects the antioxidant ability of retina, and diabetic retinopathy (DR) and to investigate the correlated factors of MPOD. METHODS:Totally 435 subjects of urban Chinese were recruited to the study and divided into 3 groups: non-diabe...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2016.10.11

    authors: She CY,Gu H,Xu J,Yang XF,Ren XT,Liu NP

    更新日期:2016-10-18 00:00:00

  • Evaluation of VEGF gene polymorphisms and proliferative diabetic retinopathy in Mexican population.

    abstract:AIM:To assess if the included vascular endothelial growth factor (VEGF) polymorphisms rs3025035, rs3025021 and rs2010963 are associated to proliferative retinopathy in a Mexican population with type 2 diabetes mellitus (T2DM). METHODS:A case-control study was conducted in adult individuals with T2DM associated to prol...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2017.01.22

    authors: Gonzalez-Salinas R,Garcia-Gutierrez MC,Garcia-Aguirre G,Morales-Canton V,Velez-Montoya R,Soberon-Ventura VR,Gonzalez V,Lechuga R,Garcia-Solis P,Garcia-Gutierrez DG,Garcia-Solis MV,Saenz de Viteri M,Solis-S JC

    更新日期:2017-01-18 00:00:00

  • Corneal topographic changes and surgically induced astigmatism following combined phacoemulsification and 25-gauge vitrectomy.

    abstract:AIM:To evaluate corneal topographic changes and surgically induced astigmatism (SIA) after combined phacoemulsification and 25-gauge transconjunctival sutureless vitrectomy (25-G TSV). METHODS:A retrospective study on 96 eyes of 87 patients who underwent combined phacoemulsification and 25-G TSV. The different topogra...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2017.01.12

    authors: Sayed KM,Farouk MM,Katome T,Nagasawa T,Naito T,Mitamura Y

    更新日期:2017-01-18 00:00:00

  • Bioinformatics analysis of microarray data to explore the key genes involved in HSF4 mutation-induced cataract.

    abstract:AIM:To reveal the mechanisms of heat-shock transcription factor 4 (HSF4) mutation-induced cataract. METHODS:GSE22362, including 3 HSF4-null lens and 3 wild-type lens, was obtained from Gene Expression Omnibus database. After data preprocessing, the differentially expressed genes (DEGs) were identified using the limma ...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.06.03

    authors: Tian R,Xu Y,Dou WW,Zhang H

    更新日期:2018-06-18 00:00:00

  • Comparison of clinical outcomes after femtosecond laser in situ keratomileusis in eyes with low or high myopia.

    abstract:AIM:To compare the clinical results of femtosecond (FS) laser in situ keratomileusis (LASIK) in high myopic patients and low myopic patients. METHODS:This study included 212 myopic eyes undergoing LASIK using a VisuMax 500kHz FS laser. All treated eyes were assigned to one of two groups according to preoperative manif...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2020.11.15

    authors: Park SH,Che CY,Kim SI,Park CY,Lee JH,Kim YH,Jung JW,Lee JS,Lee JE

    更新日期:2020-11-18 00:00:00

  • Objective evaluation of the changes in the crystalline lens during accommodation in young and presbyopic populations using Pentacam HR system.

    abstract:AIM:To quantify the changes in the lens profile with accommodation in different age groups. METHODS:The Pentacam HR system was used to obtain the images of the anterior eye segment from 23 young and 15 presbyopic emmetropic subjects in unaccommodated (with an accommodation stimulus of 0.0D) and accommodated (with an a...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2011.06.07

    authors: Ni Y,Liu XL,Wu MX,Lin Y,Sun YY,He C,Liu YZ

    更新日期:2011-01-01 00:00:00

  • Systemic changes and adverse effects induced by retinopathy of prematurity screening.

    abstract:AIM:To estimate the potential systemic events during and after retinopathy of prematurity (ROP) screening. METHODS:A prospective and descriptive designed study was conducted to detect the physiologic and pathological changes 24h before, during, and 72h after ROP screening. Control blood pressure (BP), saturation, puls...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2016.08.11

    authors: Jiang JB,Zhang ZW,Zhang JW,Wang YL,Nie C,Luo XQ

    更新日期:2016-08-18 00:00:00

  • Crosslink between lipids and acute uveitis: a lipidomic analysis.

    abstract:AIM:To explore the roles of phospholipids and sphingolipids in the inflammatory process of uveitis. METHODS:Aqueous humor (AH) and the retina were obtained from endotoxin-induced uveitis (EIU) rats during the acute inflammation stage (24h after endotoxin injection). Lipids were extracted using a modified Bligh and Dye...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.05.05

    authors: Wang HY,Wang Y,Zhang Y,Wang J,Xiong SY,Sun Q

    更新日期:2018-05-18 00:00:00

  • Analysis of the relationship between postoperative ophthalmic complications and dialysis time of pre-kidney transplantation.

    abstract:AIM:To determine the influence of the dialysis time before kidney transplantation on postoperative ophthalmic complications. METHODS:One hundred and eighty three patients who were given the follow-up after kidney transplantation were selected, including 124 males and 59 females. The dialysis time before kidney transpl...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2012.03.23

    authors: Wang YL,Qi F,Xie JL,Qi L,Zhou C,Zhu XR,Ding X,Yang B,Jin P

    更新日期:2012-01-01 00:00:00

  • The 100 most influential papers about cataract surgery: a bibliometric analysis.

    abstract:AIM:To identify the 100 most cited papers in cataract surgery, we performed a comprehensive bibliometric analysis basing on the literature search on the Thomson Reuters Web of Knowledge. METHODS:The number of citations, including the total citations, latest 5y citations and average citation number per year (ACY), auth...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2017.10.17

    authors: Lin ZN,Chen J,Zhang Q,Li Q,Cai MY,Yang H,Cui HP

    更新日期:2017-10-18 00:00:00

  • Inferior oblique weakening surgery on ocular torsion in congenital superior oblique palsy.

    abstract:AIM:To investigate changes in fundus excyclotorsion after inferior oblique myectomy or myotomy. METHODS:The records of 21 patients undergoing strabismus surgery by a single surgeon between 2009 and 2012 were examined. Only patients who had undergone an inferior oblique myectomy or myotomy, with or without horizontal r...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.3980/j.issn.2222-3959.2015.03.24

    authors: Lee J,Suh SY,Choung HK,Kim SJ

    更新日期:2015-06-18 00:00:00

  • Critical pathway for primary open angle glaucoma diagnosis.

    abstract:AIM:To develop a critical pathway for primary open angle glaucoma (POAG) diagnosis intended to be efficient, to unify criteria, reduce resource use and minimize costs to the health system. METHODS:We performed a systematic search on PubMed, Cochrane, Embase and ClinicalTrials.org databases and classified the quality o...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2017.06.21

    authors: Allocco AR,Ponce JA,Riera MJ,Magurno MG

    更新日期:2017-06-18 00:00:00

  • Fundus manifestations and HIV viral loads of AIDS patients before and after HAART.

    abstract:AIM:To investigate the fundus manifestations and human immunodeficiency virus (HIV) viral loads of acquired immune deficiency syndrome (AIDS) patients before and after highly active antiretroviral therapy (HAART). METHODS:This retrospective study included 21 AIDS patients (42 eyes) who presented to the Department of O...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2019.09.11

    authors: Di Y,Zhao XY,Ye JJ,Li B,Ma N

    更新日期:2019-09-18 00:00:00

  • A comparable study of clinical and optical outcomes after 1.8, 2.0 mm microcoaxial and 3.0 mm coaxial cataract surgery.

    abstract:AIM:To evaluate the clinical and optical outcomes after clear corneal incision cataract surgery (CICS) with three different incision sizes (1.8, 2.0 and 3.0 mm). METHODS:Eyes of 150 patients with age-related cataract scheduled for coaxial cataract surgery were randomized to three groups: 1.8, 2.0, or 3.0 mm CICS. Intr...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2016.03.13

    authors: Yu YB,Zhu YN,Wang W,Zhang YD,Yu YH,Yao K

    更新日期:2016-03-18 00:00:00

  • Modified ulcer debridement in the treatment of the superficial fungal infection of the cornea.

    abstract:AIM:To evaluate the efficacy of modified corneal ulcer debridement in superficial fungal keratitis unresponsive to medications. METHODS:A total of 209 patients (209 eyes) with fungal keratitis, involving no more than 50% of the stromal depth and not responding to antifungal agents for 2wk, were recruited in this retro...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2018.02.07

    authors: Wang JY,Wang DQ,Qi XL,Cheng J,Xie LX

    更新日期:2018-02-18 00:00:00

  • Clinical results of non-Descemet stripping endothelial keratoplasty.

    abstract:AIM:To investigate the impact of non-Descemet stripping endothelial keratoplasty (non-DSEK) on graft rejection rate, and its overall procedural effectiveness in patients. METHODS:Non-DSEK was performed on 65 eyes of 64 patients, and the procedural outcomes, including rejection episodes, failure and dislocation of the ...

    journal_title:International journal of ophthalmology

    pub_type: 杂志文章

    doi:10.18240/ijo.2017.02.07

    authors: Zhang T,Li SW,Chen TH,He JL,Kang YW,Lyu FQ,Ning JH,Liu C

    更新日期:2017-02-18 00:00:00